Production technique of andrographolide and neoandrographolide, dehydroanddrographolide, oxyandrographolide

Abstract

The invention discloses a production technique of andrographolide and neoandrographolide, dehydroanddrographolide and oxyandrographolide; the technique comprises the following steps of: firstly preparing stem and leaf extract of andrographis paniculata, removing fat-soluble impurities such as chlorophyll and the like with petroleum ether, and then hot-melting the extract in lower alcohol or aqueous lower alcohol, conducting reflux and decolorization with active carbon, separating out a majority of andrographolide crystals, then removing flavonoid through an alumina column or alkali cleaning, obtaining the andrographolide, cold-melting the andrographolide with trichloromethane or dichloromethane for 2 to 4 times, filtering and obtaining two parts of filtrate and insoluble substances, concentrating the filtrate, then conducting solvent crystallization and recrystallization or column chromatography for separation, and finally, respectively obtaining dehydroanddrographolide and pure product of andrographolide; the insoluble substances go through solvent crystallization and recrystallization or column chromatography for separation to obtain the neoandrographolide and pure product of andrographolide. The technique has simple production equipment, simplified routes and easy operation, can realize industrialized batch production; and the proportion of neoandrographolide, dehydroanddrographolide and oxyandrographolide in the obtained andrographolide is high, while the content of impurities is low. The obtained neoandrographolide, dehydroanddrographolide and oxyandrographolide all have monomer purity of higher than 98 percent, thus being capable of being used as chemical reference substance of the traditional Chinese medicine, or being applied as raw materials of medicine and chemical industry.

Description

technical field [0001] The invention relates to the extraction and separation of Chinese herbal medicines, more precisely to the production technology of neoandrographolide, dehydroandrographolide and deoxyandrographolide. Background technique [0002] Andrographis Paniculata (Burm.f.) Nees is the dry aerial part of Andrographis Paniculata (Burm.f.) Nees. Originally produced in India, it is cultivated in East China, Central South and other regions of my country. Bitter in nature, cold, Guixin, lung, large intestine, bladder channel. It has the effects of clearing heat and detoxification, cooling blood, reducing swelling and relieving pain. Clinically, it is mainly used for symptoms such as cold and fever, sore throat, aphtha of the mouth and tongue, sudden cough and overworked cough, diarrhea and dysentery, astringent pain due to hot stranguria, carbuncle and sore, venomous snake bite and other symptoms. [0003] The main components of Andrographis paniculata contain dite...

AI Technical Summary

Problems solved by technology

In this method, a lot of impurities are still mixed after the impurities are removed by the macroporous adsorption resin, and the silica gel column chromatography is directly used, the amount of solvent used is large, the separation process is complicated, and the toxic reagents chloroform and methanol are used as eluents, which is also unfavorable for operators. labor protection and environmental protection

The shortcomings of the existing production process make it difficult to buy high-purity neoandrographolide, dehydroandrographolide and deoxyandrographolide in the market, which cannot meet the needs of pharmacological activity screening and clinical application

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