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A mutanase and its use
ActiveCN119709680BLarge industrial production application potentialCatalytic conditions are mildEnzyme GeneLeuconostoc pseudomesenteroides
The present application relates to the field of enzyme engineering, and in particular to a mutanase and application thereof. At present, mutan is mostly prepared by bacterial extraction, and there are problems such as low product yield and many impurities. The present application provides a mutanase, a MutI gene fragment of the mutanase is obtained by cloning a genome of Leuconostoc pseudomesenteroides G496, and a genetically engineered bacterium is constructed. The mutanase provided by the present application is subjected to enzymatic property analysis, and it is found that the mutanase has good stability under acidic conditions, and the optimal induction expression temperature and reaction temperature are close to room temperature, and the production energy consumption is low and the raw material conversion rate is high. The catalytic product of the mutant enzyme is linear mutan with a high polymerization degree, the molecular weight of the linear mutan is as high as 650 kDa, the linear mutan contains 90% of alpha-1,3 glycosidic bonds, and the structure is relatively dense, and the linear mutan has a good application prospect in the field of biological base materials.
Owner:NANJING TECH UNIV
Method for continuously extracting and separating naringin and pectin in shaddock peel
PendingCN121851081Areduce pollutionComply with green chemistry principlesSugar derivativesSugar derivatives preparationNaringinAlcohol ethyl
The invention discloses a method for continuously extracting and separating naringin and pectin in shaddock peel. The method comprises the following steps: mixing shaddock peel powder with an alcohol amine-alcohol eutectic solvent, ultrasonically extracting naringin, and centrifugally separating to obtain a naringin extracting solution; deionized water is added into the extracting solution, CO gas is introduced, double aqueous phases are formed, and naringin is enriched in the lower phase; collecting a lower-phase solution to obtain a crude extract, heating and dissolving with ethanol, filtering, crystallizing and drying to obtain naringin; the method comprises the following steps: washing and drying residues after naringin extraction, extracting pectin by using a citric acid solution, filtering, precipitating with ethanol, centrifuging, purifying and drying to obtain the pectin. According to the method, the alcohol amine-alcohol eutectic solvent is applied to green extraction of the naringin for the first time, CO is utilized to trigger two aqueous phases to achieve efficient separation and purification of the naringin, meanwhile, multi-component high-value co-production of the naringin and pectin is achieved, the method has the advantages of being green, efficient, simple and convenient and the like, and a new way is provided for high-value utilization of shaddock peel waste.
Owner:JISHOU UNIVERSITY
Connecting assembly
ActiveCN224085502Ufacilitated releaseSimplified separation stepsHeart valvesGuide wiresMechanical engineering
The utility model discloses a connecting assembly which comprises a first connecting piece, a second connecting piece and a third connecting piece. The end of the first connecting piece is provided with a first locking part and a first positioning groove. The end part of the second connecting piece is provided with a second locking part and a second positioning groove, the first locking part is clamped and matched with the second positioning groove, and the second locking part is clamped and matched with the first positioning groove; the sleeve is used for radially restraining the first locking part and the second locking part; the first connecting piece provides a first moving space, and the second connecting piece provides a second moving space; and when the sleeve retracts to be separated from the connecting area of the first connecting piece and the second connecting piece and the first connecting piece moves relative to the second connecting piece, the first locking part moves into the second moving space, and the second locking part moves into the first moving space, so that the first locking part is separated from the second locking part. According to the utility model, the first moving space and the second moving space are provided, so that the first locking part and the second locking part can move relative to each other after the sleeve is withdrawn, and then the connection is quickly separated.
Owner:SUZHOU JIECHENG MEDICAL INC
Method for preparing two-dimensional carbon film by gallium-based alloy room temperature phase transition reducing carbon oxide
The application discloses a method for preparing a two-dimensional carbon film by reducing carbon oxides through phase transition of a gallium-based alloy at room temperature, and comprises the following steps: step S1, adding mixed metal particles containing metallic gallium into a sealed container and sealing the container; step S2, removing air in the container and replacing it with high-purity CO or CO2 gas; step S3, continuously stirring or oscillating; step S4, converting the mixed metal particles containing metallic gallium into a liquid phase at room temperature; during the liquid phase conversion of the metal particles, the CO or CO2 gas is reduced to carbon material and covers the surface of the liquid metal; and step S5, peeling off the two-dimensional carbon film, thereby obtaining the two-dimensional carbon film. The method can directly convert CO or CO2 gas into a two-dimensional carbon film with excellent performance at room temperature, can solve the environmental problem, and can realize simple preparation of the two-dimensional carbon film, so the method has a wide application prospect.
Owner:YUNNAN NORMAL UNIV
