Chiral compound separation method based on nano gold modified by aptamer

Abstract

The invention provides a chiral compound separation method based on nano gold modified by aptamer. The chiral compound separation method includes the following steps of firstly, modifying aptamer modified by mercapto onto the surfaces of nano-gold particles; secondly, adding chiral compounds to be separated, and centrifuging after incubating at room temperature to obtain supernatant which is a chiral compound of one configuration; and thirdly, dispersing the nano gold at the bottom of a centrifuge tube again, and denaturating and centrifuging the aptamer at 95 DEG C to obtain supernatant which is a chiral compound of another configuration. The nano gold modified by the aptamer serves as chiral selective reagent, the highly-selective and high-affinity characteristics are utilized, denaturation is performed at high temperature, the nano gold restores to the room temperature slowly and restores to the tertiary structure, and accordingly chiral separation of the chiral compounds is performed. A simple centrifuge is only needed for separation. In addition, targets of the aptamer are wide, so that the chiral compound separation method based on the nano gold modified by the aptamer is highly universal, operational and highly practical.

Description

technical field [0001] The invention belongs to the field of medicinal chemistry, and more specifically relates to a chiral compound resolution method based on nucleic acid aptamer-modified nano gold. Background technique [0002] The existence of chiral enantiomers is a common phenomenon in nature. Biomacromolecules, such as proteins, polysaccharides, nucleic acids, and enzymes, are almost all chiral, and these small molecules often have important functions in the body. Physiological function. During the process of absorption, distribution, metabolism and excretion, chiral enantiomers produce different pharmacological effects and reactions through different three-dimensional combinations with macromolecules in the body (J. Ruta, C. Ravelet, I. Baussanne, J. L. Décout and E .Peyrin, Analytical Chemistry , 2007, 79, 4716-4719; C. Han and H. Li, small , 2008, 4, 1344-1350.). Therefore, the separation and analysis of chiral enantiomers is of great significance to the study...

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Problems solved by technology

Aptamers have the characteristics of a wide range of targets, high affinity, high specificity in binding to targets, and high stability. They have become a new star in the field of chiral separation recently. Separation of arginine (Ruta ,J., et al. Anal. Chem.., 2006, 78, 3032), and separation of histidine as a chiral stationary phase in HPLC (Ruta ,J., et al . Journal of Chromatography B, 2007, 845, 186), etc., but the above methods generally require complex separation instruments and cumbersome and complicated column preparation techniques, and cannot achieve a large amount of separation

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