The invention relates to a hydrazone bond-connected chiral covalent organic framework bonded silica gel stationary phase and an application thereof. The stationary phase is prepared by a method comprising the following steps: uniformly mixing a hydrazide chiral precursor, 1,3,5-benzenetricarboxaldehyde and ammoniated silica gel in an organic solvent in an inert atmosphere, carrying out a reaction under the catalysis of acetic acid, and filtering, washing and drying the obtained reaction product to obtain the chiral covalent organic framework bonded silica stationary phase. The structure of the hydrazide chiral precursor is represented by formula (I) shown in the description, a mass ratio of the ammoniated silica gel to the hydrazide chiral precursor is (2-12):1, and molar ratio of the hydrazide chiral precursor to the 1,3,5-benzenetricarboxaldehyde is 1:(0.5-2). The chiral covalent organic framework-bonded silica gel stationary phase is uniform in particle size; and the stationary phase has the advantages of high column efficiency, moderate column pressure and good separation effect on cis-trans isomers and position isomers when used in high performance liquid chromatography, and also has the advantages of definite structure, simple preparation method and good batch reproducibility.