50 results about "Polyhydroxyalkanoates" patented technology

A transformed microorganism having an ability to produce a polyhydroxyalkanoate copolymer containing 3-hydroxyalkanoate monomer units having 8 or more carbon atoms includes: an exogenous gene encoding a polyhydroxyalkanoate synthase having an amino acid sequence of any one of SEQ ID NOS: 1 to 4; or an exogenous gene encoding a protein that has an amino acid sequence having a sequence identity of at least 90% with the amino acid sequence of any one of SEQ ID NOS: 1 to 4 and that has polyhydroxyalkanoate synthase activity.

The present invention relates to a fiber made from a mixture comprising an aliphatic polyester, an aliphatic-aromatic polyester and a polyhydroxyalkanoate, and to a method for producing such a fiber. The invention also relates to a filament suitable for three-dimensional printing, said filament being made from a mixture comprising an aliphatic polyester, an aliphatic-aromatic polyester and a polyhydroxyalkanoate, and to a method for producing such a filament.

The invention relates to a material, suitable for additive manufacturing and in particular for fused deposition modeling, wherein said composite material is obtained by fusion of at least two components: - at least one polymer matrix chosen among polyhydroxyalkanoates (PHA), and - at least one auxiliary filler chosen among biobased fillers consisting of biobased particles comprising lignin, cellulose and hemicellulose, wherein said biobased particles have a lignin / hemicellulose mass ratio from 1 to 6. The invention also relates to an additive manufacturing process and a product obtained by an additive manufacturing process.

The present invention relates to novel carbodiimides, to a process for their production, and to the use of the novel carbodiimides in ester-based polyols, in polyethylene terephthalate (PET), in polybutylene terephthalate (PBT), in polytrimethylene terephthalate (PTT), in copolyesters, in thermoplastic polyester elastomers (TPE E), in ethylene vinyl acetate (EVA), in polylactic acid (PLA) and / or in PLA derivatives, in polybutylene adipate terephthalate (PBAT), in polybutylene succinate (PBS), in polyhydroxyalkanoates (PHA), in blends, in triglycerides, in thermoplastic polyurethanes, in polyurethane elastomers, in PU adhesives, in PU casting resins, for PU coatings or in PU foams as stabilizers.

The present invention concerns a composition for a horticultural pot comprising a solid residue of digestate from methanization and / or a solid residue from composting and a biodegradable polymer chosen from polyhydroxyalkanoates (PHA), its preparation and its use in the manufacture of such a pot.

The technology described herein is directed to engineered chemoautotrophic bacteria and methods of producing sustainable biomolecules. In several aspects, described herein are engineered bacteria and corresponding methods, compositions, and systems for the production of products such as polyhydroxyalkanoates (PHA), sugar feedstocks, and lipochitooligosaccharide (LCO) fertilizers.

A genetically engineered strain of Pseudomonas is provided, which is capable of metabolizing lactose as a sole carbon source in the production of polyhydroxyalkanoates (PHAs). A method of engineering microorganisms and a method of PHA production are also provided, wherein lactose is used as a sole carbon source.

A biopolymer such as poly(gamma glutamic acid) 100, bacterial cellulose (200) and poly(hydroxyalkanoates) (300) synthesised from a biowaste substrate 102, (204, 304) comprising at least one of: a cell

A process for producing polyhydroxyalkanoates, comprising the steps of: fermenting production medium comprising pongamia oil using bacteria from the Cupriavidus, Burkholderia, or Alcaligenes genera capable of producing polyhydroxyalkanoates; wherein the pongamia oil is extracted from the seeds of Pongamia species and clarified by filtering before the crude, unprocessed pongamia oil is added to the production medium; and extracting the polyhydroxyalkanoates comprising a homopolymer comprising a 3-hydroxybutyrate (3HB) unit amongst other sole PHB polymers from within the bacteria.

The invention provides a method for improving the molecular weight of polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short) synthesized by microorganisms, and relates to a method for improving the molecular weight of the polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short). By knocking out or knocking down endogenous (intracellular) carboxylesterase, lipase, esterase, thioesterase and PHA degrading enzyme genes of bacteria for synthesizing PHA, the molecular weight of various synthesized PHA is obviously improved. The method has universality for improving the molecular weight of various PHAs synthesized by different species of bacteria, the mechanical property of the PHA can be improved by improving the molecular weight of the PHA, and new performance is provided for expanding the application of the PHA.

This invention discloses a biodegradable, highly bioactive magnesium bone particle, its preparation method, and its applications, belonging to the field of biomedical materials technology. Using magnesium bone particles as a matrix, an active composite coating layer comprising biodegradable polymer components and inorganic phase components is formed on its surface to construct biodegradable, highly bioactive magnesium bone particles. The biodegradable polymer components are one or more selected from polycaprolactone, polylactic acid, polylactic-co-glycolic acid copolymer, polyhydroxyalkanoates, chitosan, and their derivatives; the inorganic phase is one or more selected from hydroxyapatite, titanium dioxide, calcium phosphate materials, and combinations thereof. The preparation method includes magnesium bone particle matrix preparation, active composite layer slurry preparation, magnesium bone particle coating, and post-treatment. The prepared biodegradable, highly bioactive magnesium bone particles exhibit good degradation regulation performance and excellent bioactivity, and can be used in bone tissue engineering, bone defect repair, and scaffold filling fields.

The invention discloses a self-emulsifying bio-based polyester stabilized multi-class bio-based polymer water-based emulsion as well as a preparation method and application thereof, and belongs to the technical field of bio-based high polymer materials and water-based resin. According to the preparation method disclosed by the invention, a dual-interface synergistic stabilization mechanism is constructed by adopting the self-emulsifying bio-based polyester containing furandicarboxylic acid and the rosin-based component, so that efficient emulsification and interface compatibility of various hydrophobic bio-based film-forming polymers such as polyhydroxyalkanoate and polylactic acid are realized. The emulsion prepared by the invention has excellent storage stability and machining adaptability, maintains extremely high bio-based content and extremely low volatile organic compound residue while remarkably improving the water resistance, oil resistance and gas barrier property of a paper-based coating, and completely meets the safety standard of food contact-grade materials. According to the preparation process, the microstructure of dispersed phase particles is accurately regulated and controlled through phase inversion and high-pressure homogenization, and an ideal green coating solution is provided for high-performance environment-friendly paper-based packaging.

The present invention relates to a method for preparing polyhydroxyalkanoate nanobeads. The present invention optimizes a process in which physical cell disruption is performed in order to directly recover polyhydroxyalkanoates, produced in the cells of polyhydroxyalkanoate-producing bacteria, in the original form thereof, and the recovered polyhydroxyalkanoates are washed. Using the optimized method for preparing polyhydroxyalkanoates according the present invention has the effect that polyhydroxyalkanoates can be prepared with high purity and high yield without an organic solvent, and polyhydroxyalkanoate nanobeads can be directly obtained without a separate forming process.

The purpose of the present invention is to provide a method for producing PHA which enables efficient filtering. The above problem is solved by providing a method for producing PHA, said method comprising a step for using a filtering material having an airflow rate of 0.01-5.0 cc / cm2 / sec to perform dead-end filtration of an aqueous PHA suspension having a pH of 2.5-5.5, wherein the amount of PHA-surface-adhering protein in the aqueous PHA suspension is not more than 2,000 ppm, and the liquid density of the aqueous PHA suspension in the filtration step is 0.50-1.08 g / mL.

The invention provides a light response type curcumin antibacterial preservative film and a preparation method and application thereof, the preservative film is of a double-layer composite structure, an inner layer is a light response antibacterial layer which takes polyethyleneimine modified sodium alginate as a base material, is covalently grafted with curcumin through an amido bond and is uniformly dispersed with graphite-like phase carbon nitride (g-C3N4); and the outer layer is a protective barrier layer which takes polyhydroxyalkanoate as a base material and is loaded with nano SiO2 and VE / hydroxypropyl-beta-cyclodextrin inclusion compound. The preparation of the antibacterial film can solve the problem that curcumin is easy to migrate and lose, the efficient antibacterial effect is realized by utilizing the light synergistic effect of g-C3N4 and curcumin, and meanwhile, the outer layer film effectively prevents the damage of illumination to fruits and vegetables while ensuring the light triggering efficiency of the inner layer by scattering strong light and scavenging free radicals. The film has long-acting antibacterial, antioxidant and physical barrier functions, and is suitable for high-quality preservation of foods such as fruits, vegetables and the like.

The present invention relates to a fiber obtainable by or obtained by a process comprising the steps of: - spinning a melt of an aliphatic polyester, an aliphatic-aromatic polyester and a polyhydroxyalkanoate through a hollow fiber spinning nozzle to obtain a precursor fiber; and - cooling the precursor fiber under a temperature gradient, thereby obtaining a fiber. The present invention also relates to yarns and apparel made from such fibers and to a process for making such fibers.

Disclosed is a single-unit biotechnology method for simultaneously recovering polyhydroxyalkanoates (PHAs) and triacylglycerides (TAGs). The present invention relates to a method for producing biomass in the form of a mixed microbial culture containing PHAs and TAGs. More specifically, the method comprises certain steps that occur in a single discontinuous sequential reactor.

Compostable seaweed-based compositions, and associated systems and methods are disclosed herein. In some embodiments, the seaweed-based composition comprises (i) a phycocolloid including agar, alginate, carrageenan, and / or unprocessed seaweed, (ii) a polymer comprising thermoplastic starch (TPS), polycaprolactone (PCL), polylactic acid (PLA), polyhydroxyalkanoates (PHA), polyesteramide (PEA), polybutylene adipate terephthalate (PBAT), polybutylene succinate (PBS), and / or polyvinyl alcohol (PVOH), and (iii) an additive, wherein the phycocolloid comprises no more than 90 wt % of the seaweed-based composition, the biopolymer comprises no more than 80 wt % of the seaweed-based composition, and the additive comprises no more than 50 wt % of the seaweed-based composition.

This invention discloses a method for extracting polyhydroxyalkanoates (PHAs). The method includes the following steps: S1: adding a chelating agent and lysozyme to a bacterial suspension containing PHA-producing bacteria for reaction; S2: adding a surfactant to the reaction system of step S1 for reaction, and collecting the crude PHA product. This extraction method is carried out at room temperature, with mild reaction conditions, short reaction time, and no difficult-to-treat wastewater is generated after subsequent washing, greatly saving time and subsequent wastewater treatment costs. It is a highly efficient and green extraction process.

The present invention provides a method for producing PHA that offers excellent separation of PHA from bacterial residue (e.g., residual protein). [Solution] A method for producing PHA, comprising: (a) adding an enzyme to a culture medium containing inactivated bacterial cells that contain PHA, thereby enzymatically treating the bacterial cells; and (b) adding an oxidizing agent having a standard oxidation-reduction potential of 2.00V or higher at 25°C under alkaline conditions to the enzymatically treated solution obtained in step (a), and reacting it under conditions of pH 9.0 to 12.0.

A poly(3-hydroxyalkanoate) resin composition for molding contains a poly(3-hydroxyalkanoate) copolymer (A) having a weight-average molecular weight of 10×104 to 100×104 and a poly(3-hydroxybutyrate) resin (C) having a weight-average molecular weight of 16×104 to 50×104. The amount of the resin (C) is from more than 5 to 50 parts by weight per 100 parts by weight of the copolymer (A).

The invention relates to the field of polymer composite materials, and discloses a preparation method of a biodegradable PBS / PLA / PHA composite material. Polybutylene succinate is used as a rigid framework, polylactic acid and medium-and-long-chain polyhydroxyalkanoate are used as flexible phases, and nano-montmorillonite grafted and modified by poly (butylene succinate)-co-citric acid oligomer and core-shell structure modified lignin with polycaprolactone-co-butylene succinate as a shell layer are introduced. A four-order double-screw gradient extrusion process is adopted, material pre-plasticizing, nano particle in-situ stripping, ester exchange reaction compatibilization and vacuum devolatilization are sequentially realized through combination of partitioned temperature control and screws, and the problem of multiphase interface compatibility is effectively solved. The composite material has excellent mechanical properties, high light transmittance and controllable biodegradation rate, the tensile strength of a film product of the composite material can reach 38MPa or above, the elongation at break reaches 500%, the light transmittance is greater than 90%, and the soil degradation rate in 180 days exceeds 96%.

The present invention relates to a method for producing polyhydroxyalkanoate (PHA) and a method for monitoring cell disruption. The present invention provides a method for producing polyhydroxyalkanoate (PHA) that enables the length and sphericity of cells or PHA to be measured by three-dimensional image analysis utilizing the difference in refractive index of the cells or PHA, thereby identifying the degree of autolysis, supply state, or degree of disruption of PHA cells, thereby enabling optimal disruption conditions to be set and enhancing the stability of the impurity removal process, which is a process subsequent to the disruption process.

This invention relates to the field of biomass polymer materials technology, and more particularly to polyhydroxyalkanoate compositions, polyhydroxyalkanoate molded articles, and methods for producing the same. The polyhydroxyalkanoate composition according to the present invention comprises a polyhydroxyalkanoate and at least one selected from lactic acid and lactic acid organic derivatives. By using lactic acid or its derivatives as a nucleating agent, the crystallization rate and crystallinity during the production of polyhydroxyalkanoate molded articles can be significantly improved, and the crystallization temperature of polyhydroxyalkanoates, which have a wide melting temperature range, can be lowered, effectively shortening the solidification time of the molded articles. This provides advantages such as high nucleating agent efficiency, a simpler processing method, and reduced energy consumption.

The present invention is in the field of additive manufacturing. Positive models of one or more teeth and / or gums suitable for manufacturing dental aligners are provided, the models comprising polyhydroxyalkanoates (PHAs). Such models are also provided by sintering or fusing powders comprising polyhydroxyalkanoates (PHAs).

A method for depolymerizing a poly(hydroxyalkanoate) by reaction with a depolymerase enzyme to produce a mixture of poly(hydroxyalkanoate) degradation products. The present disclosure also provides methods for recycling or repurposing the poly(hydroxyalkanoate) to provide a new poly(hydroxyalkanoate) or to produce a biogas feedstock.

This invention discloses an easily recyclable and biodegradable water-based ink binder system, its preparation method, water-based ink, and its applications, belonging to the field of environmentally friendly ink technology. This system utilizes the synergistic effect of polyhydroxyalkanoates and alkali-triggered debonding resin to construct a unique pH-responsive mechanism. Compared with traditional inks, this invention exhibits superior physical properties during printing, and can be rapidly peeled off from paper or plastic surfaces in solid fragments during recycling. This unique "debonding" mode not only significantly improves deinking efficiency and washing cleanliness but also effectively prevents water pollution caused by resin dissolution, solving the pain point of high chemical oxygen demand (COD) load in existing technologies. This solution balances the high adaptability of commercial printing with the environmental friendliness of packaging waste throughout its entire life cycle, achieving a green closed loop from source carbon reduction to end-of-life harmless treatment.