The invention relates to an organic fertilizer composition prepared from tenebrio molitor manure. The organic fertilizer composition is prepared from the following raw materials in percentage by mass: 65-75% of tenebrio molitor manure, 8-10% of loess, 8-10% of tobacco stalks, 4-6% of vermiculite, 4-6% of perlite and 1-3% of original liquid of EM (Effective Microorganisms), wherein the sum of the content of the above ingredients is 100%. The invention further relates a preparation method of the organic fertilizer composition. The method comprises the steps of firstly weighing the raw materials, then, crushing and screening the raw materials, then, adding materials in an obtained mixture, then, fermenting the mixture, then, adding materials, and finally, extruding the fermented material by a dry extrusion granulator, thereby obtaining the organic fertilizer composition. The organic fertilizer prepared by the method has the advantages that the fertility can meet the requirements on various plant required elements, the problem of land pollution caused by the use of chemical fertilizers is avoided, the fertilizer effect can be maintained in soil for a long term, the growth of crops is promoted, the waste is sufficiently utilized, the waste is changed into the valuable, and the pollution to the environment is reduced.