The invention provides a flame-retardant single-component moisture-curable silicone rubber and a preparation method and application thereof and belongs to the field of organic silicon rubber materials. The preparation method of the flame-retardant single-component moisture-curable silicone rubber is characterized in that alpha,omega-hydroxyl-terminated polydimethylsiloxane serves as a basic resin, alkoxy silane serves as a cross-linking agent, organic tin serves as a catalyst, meanwhile reinforcing filler, increment filler, a fire retardant, hydroxyl scavenging agent and other components are adopted for matching, and the silicone rubber is obtained through vacuum kneading and mixing, extrusion and lamination and vacuum package. The silicone rubber is a single-component band-shaped or flaky material, is stable in appearance, has a certain initial strength and flexibility, and has good insulativity, weather resistance, heat resistance, acid-base resistance and flame retardant properties after moisture curing. The silicone rubber has good appearance and plasticity, can be attached in a planar mode, wound and bound, or be plugged, blocked and filled in joints and has the wide application prospect in the fields of cable damage repairing, insulated protection of exposed electrical connecting points and the like. The flame-retardant single-component moisture-curable silicone rubber is simple in preparation process and is especially suitable for industrial production, and the raw materials are wide in source.