33 results about "Propiolactone" patented technology

The invention provides bovine ephemeral fever virus positive serum as well as a preparation method and application thereof. The preparation method comprises the following steps: culturing bovine ephemeral fever virus by using MDBK suspension cells, inactivating by using beta-propiolactone, concentrating by using a 500kd hollow fiber column to obtain an antigen solution, and emulsifying the antigen solution and 61VG adjuvant according to a mass ratio of 1: 1.5 to prepare the vaccine; carrying out primary immunization and secondary immunization on the receptor, collecting blood of which the serum neutralizing antibody titer is greater than 1: 4096 14-21 days after the secondary immunization, and separating to obtain positive serum. The positive serum can be used for specific test, identification test and the like of the bovine ephemeral fever virus, can also be used for preparing related reagents, kits and standard substances, and provides efficient and normative technical support for prevention and control of bovine ephemeral fever.

The invention belongs to the technical field of rabies vaccines, and particularly relates to a purification method of a rabies virus inactivated vaccine, which comprises the following steps: step 1, carrying out passage by using a Walvax-2 cell strain through a cell factory method, and carrying out multiplication culture on the passage production cells in a microcarrier bioreactor; 2, cleaning the cells with a treating fluid, inoculating the cells with a rabies virus fixed strain, and preparing and culturing a rabies virus solution; step 3, carrying out ultrafiltration concentration on the rabies virus stock solution; step 4, purifying the rabies virus concentrated solution; step 5, inactivating the rabies virus; and 6, hydrolyzing to obtain the rabies vaccine stock solution. By adopting the composite Capto Core 700 column chromatography, impure proteins can be effectively removed, the recovery rate is high, the quality of the rabies vaccine obtained by adopting a mode of firstly purifying and then adopting beta-propiolactone inactivation is excellent, and the effect of the finally obtained human diploid cell rabies vaccine stock solution is good.

The method includes contacting one or more beta-propiolactone monomers with one or more phosphorus compounds in a solution under conditions such that a polypropiolactone polymer is formed. The solution includes one or more polar protic solvents and one or more phosphorus compounds. The phosphorus compounds contain phosphorus in ionic form, either covalently bound to another compound to form a zwitterion or ionically bound to another compound to form a salt.

The present invention discloses a canine coronavirus attenuated strain CCoVAS22 and its application, and relates to the field of biomedicine technology. The canine coronavirus attenuated strain CCoVAS22 was deposited in the China Center for Type Culture Collection on July 12, 2024, and the deposit address is Wuhan University, Wuhan, China, and the deposit number is CCTCC NO: V202413. The present invention utilizes the technical means of cross-passaging in MDCK and CRFK cell lines to obtain an independently isolated CCoVAS22 strain, which has the advantages of high toxicity and stable passage on cells. After inactivation by β-propiolactone, it has the characteristics of high safety and good immunogenicity. It can be used as a candidate strain for CCoV inactivated vaccine, which can alleviate the problem of the small number of canine coronavirus isolates in China to a certain extent, and provide technical support for the prevention of diseases caused by canine coronavirus.

The application provides a production device and process for synthesizing propiolactone based on homogeneous and heterogeneous composite catalysis, and relates to the technical field of propiolactone preparation. The propiolactone production device comprises a dehydration polycondensation reaction tank, a peristaltic pump, a pyrolysis reaction tank, a condensation tank, a finished product tank and a negative pressure conveying pump connected in sequence, wherein a homogeneous catalyst adding pipe is arranged on the dehydration polycondensation reaction tank; the pyrolysis reaction tank comprises a tank body, two layers of mesh plates are arranged in the tank body, and the two layers of mesh plates divide the tank body into an upper cavity, a middle cavity and a lower cavity; the upper cavity is connected with the condensation tank, a distributor is arranged in the upper cavity, and a liquid inlet end of the distributor is connected with a liquid outlet end of the dehydration polycondensation reaction tank; a heater and a heterogeneous catalyst are arranged in the middle cavity. The heterogeneous catalyst of the application is helpful for rapid vaporization of volatile components, and has the double advantages of homogeneous catalysts and heterogeneous catalysts, so that the overall reaction efficiency of lactic acid oligomers is effectively improved.

The present invention relates to the technical field of biologics, and in particular to a method for preparing a Streptococcus pneumoniae capsular polysaccharide. The present invention provides a method for preparing a Streptococcus pneumoniae capsular polysaccharide. The method comprises: using β-propiolactone to treat a Streptococcus pneumoniae fermentation culture, separating and collecting a supernatant, purifying the supernatant, and collecting a capsular polysaccharide. In the present invention, β-propiolactone is used to treat the Streptococcus pneumoniae fermentation culture, thereby reducing residual impurities such as proteins, and achieving relatively high polysaccharide yield; in addition, the β-propiolactone is susceptible to hydrolysis, and the hydrolysate is non-toxic and harmless and has higher safety. The Streptococcus pneumoniae capsular polysaccharide prepared by the method of the present invention have quality control indexes meeting requirements, and can be used for preparing a Streptococcus pneumoniae capsular polysaccharide vaccine and a polysaccharide conjugate vaccine.

This invention discloses a method for preparing heteroatom porous beta molecular sieves, comprising the following steps: (1) mixing deionized water, tetrahydrofuran, sodium oxide, sodium aluminate, and zwitterionic polymer PAAn- b - PILm is mixed and stirred evenly, and tetraethyl orthosilicate is added dropwise. The mixture is stirred at 35-45℃ for 8-16 hours; (2) it is transferred to a reaction vessel lined with polytetrafluoroethylene, crystallized at 75-85℃ for 36-60 hours, and then crystallized at 130-150℃ for 8-16 hours; then dried and calcined. At the same time, the present invention also discloses the Sn-beta molecular sieve catalyst for preparing lactide using the beta molecular sieve and its preparation method and its application in the preparation of lactide. The reaction conditions of the present invention are mild and suitable for high concentrations of lactic acid raw materials, which reduces the production cost and produces lactide with high purity, thus solving the problems existing in the two-step preparation of lactide.

A method includes contacting one or more beta-propiolactone monomers and one or more phosphorous compound in a solution under conditions such that a polypropiolactone polymer is formed. The solution comprises one or more polar protic solvents and the one or more phosphorous compounds. The phosphorous compound comprises phosphorous in an ionic form that is either covalently bound to another compound to form a zwitterion or ionically bound to another compound to form a salt.

This invention provides a method for refining lactide, comprising: condensing and crushing crude lactide into solid lactide powder; using a mixed solvent of water and low-carbon alcohol as a washing solvent; mixing the solid lactide powder with the powder at a temperature not exceeding 20°C; separating the powder; washing the filter cake; and drying to obtain refined lactide. This invention creatively breaks through the inherent thinking of existing technologies by mixing lactide in solid form rather than in molten form with the washing solvent. This slows down the hydrolysis of m-lactide in existing methods and avoids the temperature rise caused by the molten state and the uneven particle size distribution and easy agglomeration of the molten material after cooling in the washing solvent. The resulting lactide has high purity, high yield, and uniform particle size distribution.

The application belongs to the technical field of nano zeolite, and particularly relates to a kind of nanometer spherical multi-level hole H-Beta zeolite and its preparation method and application.Nanometer spherical multi-level hole H-Beta zeolite of the application uses silicon source, aluminum source and microporous template agent as raw material, is obtained by controlling raw material ratio and preparation process, has micropore and double intercrystalline mesopore structure, has large external specific surface area (431~670m 2 / g) and mesopore volume (0.25~0.57cm 3 / g);The double intercrystalline mesopore structure of H-Beta zeolite of the application improves the mass transfer limitation caused by traditional zeolite only having micropore, and avoids weakening of the limitation caused by forming macropore;In the reaction of catalyzing lactic acid to synthesize propiolactone, excellent catalytic activity and stability are presented, and the generation of byproduct can be reduced, and the yield of product is improved.

The invention discloses a thiourea acylthiourea metal complex catalyst, a preparation method thereof and application of the thiourea acylthiourea metal complex catalyst in lactide ring-opening polymerization. The preparation method comprises the following steps: (1) condensing 3, 5-bis (trifluoromethyl) phenyl isothiocyanate and para aminobenzoic acid in dichloromethane to obtain a carboxyl-containing product A; (2) performing acylating chlorination on the product A to obtain an intermediate B; (3) reacting the intermediate B with ammonium thiocyanate to generate an isothiocyanate intermediate C; (4) reacting the intermediate C with chiral primary amine to obtain a ligand intermediate D with a chiral center; and (5) coordinating the ligand intermediate D with a zinc compound or a titanium compound in an organic solvent to obtain the target catalyst. Molecules of the catalyst contain a chiral thiourea unit and an acylthiourea metal chelating unit at the same time, so that a monomer and an initiator can be activated synergistically. When being used for ring opening polymerization of lactide, the polylactic acid with high stereoregularity and narrow molecular weight distribution can be efficiently prepared at room temperature; the carbon nanotubes are added into a reaction system, so that the polylactic acid composite material with conductivity can be obtained.

The invention provides a high-temperature-resistant zwitterionic polymer tackifier for a composite calcium salt completion fluid as well as a preparation method and application of the high-temperature-resistant zwitterionic polymer tackifier. The preparation method of the polymer tackifier comprises the following steps: adding hydroquinone into an isocyano ethyl methacrylate solution, adding the obtained mixed solution into a dimethylaminoacethydrazide solution, and reacting; centrifuging, washing and drying to obtain an intermediate monomer; adding the intermediate monomer and hydroquinone into tetrahydrofuran, stirring and dissolving, adding beta-propiolactone, and reacting to obtain a zwitterionic monomer A; the preparation method comprises the following steps: adding a temperature-resistant anionic monomer, an acrylamide monomer, a zwitterionic monomer A and a temperature-resistant cationic monomer into deionized water, uniformly stirring, adjusting the pH value of the system to 7-8, introducing nitrogen to remove oxygen, heating to a reaction temperature, adding an initiator, and reacting to obtain the temperature-resistant water-soluble polymer. The tackifier provided by the invention can resist high temperature and composite calcium salt, and can significantly improve the viscosity of the completion fluid under a high temperature condition.

The present application relates to a kind of stable chlorpyrifos technical and the application of stabilizer, the content of chlorpyrifos in the chlorpyrifos technical is 92.0-99.5%, chlorpyrifos technical contains stabilizer, stabilizer is p-hydroxyanisole, beta-propionolactone, or the composition consisting of p-hydroxyanisole and beta-propionolactone, wherein the mass content of stabilizer in chlorpyrifos technical is 0.05 ‰-5.0 ‰.The present application uses p-hydroxyanisole, beta-propionolactone or the composition consisting of p-hydroxyanisole and beta-propionolactone as the stabilizer of chlorpyrifos technical solves the stability problem of chlorpyrifos technical, especially can effectively reduce the decomposition of active ingredient in chlorpyrifos technical.

Disclosed are methods of efficiently making poly(3-hydroxypropionate) and related copolymers from beta propiolactone utilizing carboxylate salts of an onium cation. In another aspect, the present invention provides polymerization systems comprising combinations of initiators and monomers that together enable the efficient production of poly(3-hydroxypropionate) and related copolymers. Disclosed are novel polymer compositions having structures and / or compositional characteristics that differentiate them from previously produced polymers and polymer compositions.

The invention provides a preparation method of a conductive antifouling material and application of the conductive antifouling material in an electrochemical biosensor, and the preparation method comprises the following steps: dissolving 2-thiophenyl chloride in anhydrous acetonitrile, dropwise adding N1, N1-dimethylethane-1, 2-diamine into the solution, separating, drying and purifying to obtain an intermediate S1; in an argon environment, beta-propiolactone is dropwise added into a solution of the intermediate S1 in anhydrous THF, and the zwitter-ion thiophene material is obtained after extraction, drying and purification; the preparation method comprises the following steps: mixing a zwitterionic thiophene material with graphene oxide, and performing ultrasonic treatment and stirring treatment to prepare a GO / Th-Zw mixed solution; and carrying out one-step electrochemical deposition on the GO / Th-Zw mixed solution to prepare the conductive antifouling material. The GO / Th-Zw composite material prepared by the invention can enhance the conductivity of the surface of an electrode, can effectively prevent non-specific adsorption of impure proteins, and ensures the accuracy of detecting a target object in a complex environment by electrochemical biosensing.

The application discloses a kind of zwitterionic compounds and its preparation method and the preparation method of the contact lens with antifouling function. First, diisocyanate compound is reacted with hydroxyl-containing acrylate compound to obtain urethane-containing acrylate isocyanate half-enclosure, then it is reacted with active hydrogen-containing tertiary amine to obtain urea group, urethane group-containing acrylate monomer, and then zwitterionic is realized by β-propiolactone ring opening. The application also combines the obtained zwitterionic compound monomer to the contact lens by free radical initiation polymerization, improves the hydrophilicity of the lens, and obtains the contact lens with excellent antifouling function. The structure of this hydrogen bond, zwitterionic crosslinking network avoids the vicious development of contact lens biofouling adhesion, and is a promising strategy to solve the problem of contact lens biofouling adhesion.

The invention discloses a histophilic somniphilus, a hemagglutination antigen, a hemagglutination inhibition antibody detection method, a kit and application of the histophilic somniphilus and the hemagglutination antigen. The method comprises the following steps: screening a histophilic sleep bacteria Yunnan isolate ZY25055 with high hemagglutination activity, culturing, and carrying out beta-propiolactone inactivation to prepare the histophilic sleep bacteria hemagglutination antigen. 1% hydroformylated sheep red blood cells are prepared by collecting sheep blood. Immunizing goat posterior jugular vein blood sampling with beta-propiolactone inactivated histophilic sleep bacteria liquid for three times, and centrifugally separating serum to prepare histophilic sleep bacteria standard positive serum. The method and the kit for detecting the histophilic sleeping bacteria hemagglutination inhibition antibody are established by utilizing the prepared histophilic sleeping bacteria hemagglutination antigen, 1% hydroformylated sheep red blood cells and histophilic sleeping bacteria standard positive serum, the method has no cross reaction with positive serum of common pathogens of cattle and sheep, and the method has the advantages of strong specificity, simplicity and convenience in operation, no need of complex equipment and the like, and can be used for detecting the histophilic sleeping bacteria hemagglutination inhibition antibody. The kit can be used for detecting the histophilic somniphilus antibody in bovine serum, sheep serum and goat serum.

The application discloses a preparation method of a non-metal catalyst for synthesizing propiolactone by a two-step method. The method comprises the following steps: preparing a grafted monomer with a carboxylic acid group by a Friedel-Crafts acylation reaction of an acid anhydride and an aromatic hydrocarbon; preparing a PP matrix by a thermal-initiated grafting reaction of styrene to a PP molecular chain; and connecting the grafted monomer with the carboxylic acid group and the PP matrix by a Friedel-Crafts alkylation reaction by using dimethoxymethane as an alkylation reagent, so as to obtain a PP grafted copolymer catalyst which can be used for synthesizing propiolactone by a two-step method. In the preparation method, no peroxide initiator is used, and the prepared PP grafted copolymer has the advantages of large molecular weight and high carboxyl content, and can be used for catalyzing and synthesizing propiolactone.

The application discloses a rhabdovirus-free Sf9 cell screening method of double pretreated conditioned medium, relates to the technical field of biology, and obtains the rhabdovirus-free Sf9 cell by double pretreatment of the conditioned medium through cross-flow filtration and beta-propiolactone inactivation and hydrolysis, and combination of single cell cloning screening. The method realizes efficient virus interception and high-activity retention of nutritional factors, the single cell cloning formation rate is greater than 40%, and is suitable for virus-free cell bank construction and biological product production.

A method for efficiently making polypropiolactone and related copolymers from betapropiolactone utilizing carboxylate salts of onium cations is disclosed. In another aspect, the present invention provides a polymerization system comprising a combination of initiators and monomers that together enable the efficient production of polypropiolactone and related copolymers. Novel polymer compositions are disclosed that have structural and / or compositional properties that distinguish them from previously produced polymers and polymer compositions.

The invention provides a preparation method and application of a nitrogen-doped carbon-loaded palladium catalyst, specifically, the nitrogen-doped carbon-loaded palladium catalyst is prepared by an impregnation method, and can efficiently catalyze ketene and carbonyl compounds to generate a gas phase [2 + 2] reaction to simply and efficiently prepare a series of beta-propiolactone derivatives. Firstly, the structure and the preparation method of the nitrogen-doped carbon-loaded palladium catalyst are novel, an assistant metal salt, palladium and assistant metal are introduced for concerted catalysis, ketene and carbonyl compound gas phase [2 + 2] reaction can be efficiently catalyzed, and the nitrogen-doped carbon-loaded palladium catalyst has good potential application value; secondly, the gas phase [2 + 2] cycloaddition reaction is adopted, the defects that an existing liquid phase process is large in solvent dosage, high in cost and the like are overcome, the preparation cost of the beta-propiolactone can be reduced, and wide application prospects are achieved.

Methods of efficiently preparing polypropiolactone and related copolymers from beta-propiolactone using carboxylate salts of onium cations are disclosed. In another aspect, the present invention provides a polymerization system comprising a combination of an initiator and a monomer that together are effective to produce polypropiolactone and related copolymers. Disclosed are novel polymer compositions having structural and / or compositional characteristics that make them distinct from previously produced polymers and polymer compositions.

The invention belongs to the field of synthesis of biodegradable materials, and particularly relates to a beta molecular sieve catalyst, preparation thereof and application of the beta molecular sieve catalyst in catalyzing lactic acid liquid-phase one-step method to prepare lactide. When the catalyst is used for synthesizing a beta molecular sieve, Lewis acidic metal is introduced by adopting an in-situ method, so that the beta molecular sieve catalyst with the total acid content of 490-550 [mu] mol / g is obtained. According to the present invention, the acidity ratio in the beta molecular sieve is regulated and controlled by using the in-situ metal introduction method according to the acidity range required by the one-step synthesis of lactide from lactic acid, and compared with the post-introduction of the metal component, the high dispersion of the metal in the beta molecular sieve can be achieved, and the anchoring of the metal is achieved through the chemical bond so as to improve the stability of the catalyst; when the catalyst is used for catalyzing lactic acid to synthesize lactide, the lactide with high optical purity can be obtained with high selectivity, the purification difficulty of the lactide is reduced, the liquid-phase one-step method is operated under normal pressure / near normal pressure, the production condition is mild, and the catalyst is easy to separate and recover.

The invention provides polyhydroxyalkanoate and a synthesis method thereof. The polyhydroxyalkanoate comprises a chain segment which is generated by carrying out ring-opening polymerization reaction on a monomer combination, the monomer combination is composed of at least two monomers selected from the group consisting of R type beta-butyrolactone, S type beta-butyrolactone, R type alpha-methyl-beta-propiolactone and S type alpha-methyl-beta-propiolactone; and the monomer combination is not a raceme of R type beta-butyrolactone and S type beta-butyrolactone or a raceme of R type alpha-methyl-beta-propiolactone and S type alpha-methyl-beta-propiolactone. The polyhydroxyalkanoate disclosed by the invention is relatively high in mechanical strength, relatively good in toughness and relatively good in appearance; the synthesis method provided by the invention is relatively low in cost, relatively mild in reaction condition and relatively short in reaction time.

Disclosed are methods of efficiently making poly(3-hydroxypropionate) and related copolymers from beta propiolactone utilizing carboxylate salts of an onium cation. In another aspect, the present invention provides polymerization systems comprising combinations of initiators and monomers that together enable the efficient production of poly(3-hydroxypropionate) and related copolymers. Disclosed are novel polymer compositions having structures and / or compositional characteristics that differentiate them from previously produced polymers and polymer compositions.

Disclosed are methods of efficiently making polypropiolactone and related copolymers from beta propiolactone utilizing carboxylate salts of an onium cation. In another aspect, the present invention provides polymerization systems comprising combinations of initiators and monomers that together enable the efficient production of polypropiolactone and related copolymers. Disclosed are novel polymer compositions having structures and / or compositional characteristics that differentiate them from previously produced polymers and polymer compositions.

The invention discloses a binder, a pole piece, and an electrochemical device and an electronic device comprising the same, and belongs to the technical field of energy storage. The binder comprises a polymer, a conductive agent and a lactone slow-release acidifier, wherein the conductive agent comprises at least one of a carbon material and a metal oxide; the polymer comprises sodium alginate and polyacrylic acid; the lactone slow-release acidifying agent is prepared from at least one of gluconic acid-delta-lactone, beta-propiolactone and epsilon-caprolactone. The application can weaken the sliding of the linear structure of the binder, enhance the conductivity, improve the inhibition of the binder on the expansion of the silicon-based negative electrode, and effectively improve the cycle performance of the electrochemical device.

The present invention discloses a kind of amido urea zwitterion hydrogel with high strength and self-healing and its preparation method, first by (methyl) acrylate isocyanoethyl and dimethylamino hydrazide reaction obtain the vinyl intermediate containing tertiary amino group, further with β-propiolactone or 1,3-propane sultone reaction obtain (methyl) acrylic acid amido urea zwitterion betaine monomer, finally by free radical initiation (light initiation or heat initiation) polymerization obtain amido urea zwitterion hydrogel. The hydrogel can be gelled without crosslinking agent, and has high strength, self-healing and excellent antifouling characteristics. After the gel is prepared into lyophilized powder by extrusion lyophilization, it can be stored for a long time, and after redissolving in deionized water or phosphate buffer solution, it can immediately form a gel with excellent injectability, and it has huge application prospects in the biological fields such as cryopreservation of cells, drug packaging, and postoperative anti-adhesion.

A method is for preparing acrylic acid from β-propiolactone and for using β-propiolactone. The process is based on a specific reactivity of β-propiolactone whereby acrylic acid is formed under operating conditions that are mild, especially in terms of temperature.