53 results about "Dichlorophenoxyacetic acid" patented technology

The invention relates to the field of cosmetics, in particular to a nicotiana benthamiana callus extract, a preparation method thereof and cosmetics. The method comprises the following steps: inoculating an induction medium with young leaves of nicotiana benthamiana as explants, and inducing the explants to form calluses; the induction culture medium is prepared from 1.0 to 3.0 mg / L of 6-benayl aminopurine; 0.2 to 1.0 mg / L of naphthylacetic acid; inoculating the callus on a subculture medium for multiplication culture; the subculture medium is prepared from the following components: 0.5 to 2.0 mg / L of 6-benayl aminopurine, 0.0 to 1.0 mg / L of naphthylacetic acid and 0.0 to 2.0 mg / L of 2, 4-dichlorphenoxyacetic acid; and extracting the calluses of the nicotiana benthamiana after multiplication culture by using water as a solvent according to a material-liquid ratio of 1: (5-20). According to the method, the Bensi tobacco callus with high dedifferentiation degree, good loosening degree and high growth speed is obtained, and the callus extract with the DPPH removal rate reaching 85% or above is obtained through extraction.

The invention belongs to the technical field of pesticides, and discloses a heterocarboxylic acid dimer as well as a preparation method and application thereof. Herbicides such as 2, 4-dichlorphenoxyacetic acid, 2-methyl-4-chlorophenoxyacetic acid and fluroxypyr acting on a TIR1 target point and herbicides such as dicamba and halauralid acting on an AFB5 target point are connected through a connecting arm containing groups such as O, S and N, so that the heterologous carboxylic acid dimer is constructed. The herbicidal activity and crop safety determination results show that compared with the single use or mixed use of the parent compound, the synthesized heterocarboxylic acid dimer has higher herbicidal activity and better crop safety. In addition, the preparation method of the heterologous carboxylic acid dimer is safe, simple and feasible.

The application provides a genetic transformation method of sugarcane variety Liucheng 1541 and belongs to the technical field of plant genetic engineering. The genetic transformation method is that leaf tissues of the sugarcane variety Liucheng 1541 are induced and cultured on a callus induction culture medium containing 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine; the obtained embryogenic callus is infected by agrobacterium; after co-cultivation and recovery culture, the obtained recovered callus is transferred to a screening culture medium containing glufosinate ammonium for screening culture; then the screened resistant embryogenic callus is inoculated on a differentiation culture medium containing glufosinate ammonium for differentiation culture; and after rooting culture, the transgenic plant of Liucheng 1541 is obtained. The method improves the embryogenic callus induction rate, solves the problem of callus browning in the tissue culture process of the variety Liucheng 1541, determines the glufosinate ammonium screening concentration of the embryogenic callus, and establishes the genetic transformation system of Liucheng 1541.

The invention relates to a preparation method of a fluorescence sensor. The invention relates to a preparation method of a portable pesticide detection fluorescence sensor of silver nanocluster (AgNCs) at zeolite imidazolate skeleton (ZIF) at polydopamine (PDA) and application of the portable pesticide detection fluorescence sensor in on-site rapid detection of 2, 4-dichlorophenoxyacetic acid (2, 4-D), and belongs to the technical field of fluorescence sensors. The method comprises the following steps: wrapping AgNCs with ZIF, and modifying the surface of the AgNCs with dopamine to form an AgNCs-ZIF-PDA composite material with high stability, high fluorescence enhancement, high enzyme responsiveness and high controllability; then, the material is integrated into a nano clay-acrylamide hydrogel matrix with high fitness, and a sensor with an adjustable shape is constructed. The sensor can be adaptively attached to the surface of a plant and is used for long-term in-situ monitoring of 2, 4-D pesticide. And in combination with an image processing algorithm, the system realizes accurate tracking of the tomato plant surface 2, 4-D degradation process, and the detection limit reaches 23.3 ng / mL. According to the invention, non-intrusive and low-cost technical support is provided for crop health management and sustainable precision agriculture.

The application discloses a stenotrophomonas maltophilia capable of degrading 2,4-dichlorophenoxyacetic acid, and a stenotrophomonas maltophilia strain GDUTXIONG3 is screened, wherein the strain GDUTXIONG3 is preserved in the China Center for Type Culture Collection on October 26, 2022, and the preservation number is CCTCC NO: M 20221673. The application discloses, for the first time, the degradation effect of the stenotrophomonas maltophilia on 2,4-dichlorophenoxyacetic acid, and the degradation rate of the strain on 50 mg / L of 2,4-dichlorophenoxyacetic acid can reach 82.9% or more within 96 hours. The GDUTXIONG3 strain is pollution-free and harmless in use, and can be used for repairing and treating an environment polluted by 2,4-dichlorophenoxyacetic acid.

The invention discloses a ferrocenyl chiral diphosphine ligand and a preparation method of an intermediate of the ferrocenyl chiral diphosphine ligand, and belongs to organic synthesis. The method comprises the following steps: dissolving acetylferrocene and a chiral catalyst in an organic solvent, dropwise adding dimethylamine, after the reaction is finished, purifying to obtain a crude product of N, N-dimethyl-1-ferrocenylethylamine, and splitting and dissociating to obtain (S)-(-)-N, N-dimethyl-1-ferrocenylethylamine. The preparation method comprises the following steps: dissolving 2, 4-dichlorophenoxyacetic acid into an organic solvent, dropwise adding sec-butyllithium under a low-temperature condition, continuing to react, dropwise adding an organic solvent solution of iodine under a low-temperature condition, and continuing to react to obtain an iodinated product; then carrying out Ullmann reaction on the iodinated substance under the catalysis of nickel to obtain a coupling product; and finally, dissolving the coupling intermediate and diphenylphosphine in acetic acid, and continuously reacting to obtain a target product. The preparation method provided by the invention has the advantages of cheap and easily available raw materials, less chiral catalyst dosage, less preparation steps, high product yield and good product quality.

The invention relates to the technical field of plant tissue culture, in particular to a medium-grain coffee embryonic callus induction method which comprises the following steps: selecting medium-grain coffee leaves as explants for surface sterilization; sequentially carrying out three culture stages, namely a dedifferentiation stage, an embryogenic induction stage and an embryogenic maintenance stage, on the sterilized explant; in the dedifferentiation stage, a first culture medium added with 2, 4-dichlorphenoxyacetic acid is adopted, and culture is performed under a first culture condition; in the embryogenic induction period, a second culture medium added with 2, 4-dichlorphenoxyacetic acid and 6-benzyladenine is adopted, and culture is performed under a second culture condition; in the embryogenic maintenance period, a third culture medium added with 1-naphthylacetic acid and kinetin is adopted, and culture is performed under a third culture condition; according to the method disclosed by the invention, the compact embryonic callus with low waterlogging degree is obtained, and the induction rate of the embryonic callus reaches 85-95% through three-stage progressive hormone regulation and control, which is obviously higher than that of the prior art by 30-50%.

The present invention discloses a molecular imprinting dual-wavelength portable photoelectrochemical sensor and its preparation method and application, belonging to the field of sensor technology. The present invention adopts gold nanoparticles (Au NPs) to modify porous silicon and target analyte (2,4-dichlorophenoxyacetic acid (2,4-D)) as photoelectrochemical active substances, and generates non-interfering photocurrent signals at two different excitation wavelengths (560 and 280nm), and uses the falling and rising photocurrent signals generated at different excitation wavelengths under different analyte concentrations as ratio analysis signals, and the differential response between the imprinted polymer and the non-imprinted polymer as the analysis signal, thereby realizing high sensitivity and high selectivity detection of 2,4-D. The ratio differential molecular imprinting photoelectrochemical technology can also be used as an innovative and universal method for detecting other MIPs targets with similar properties.

According to the missible oil preparation containing the fluroxypyr-mepthyl and the 2, 4-dichlorphenoxyacetic acid and the preparation method of the missible oil preparation, the components and the proportion of an emulsifier are optimized, the screened composite emulsifier is good in emulsification effect, the particle size of the emulsion is smaller than 1 micrometer after the emulsion is diluted in water, the emulsion is more evenly distributed on crops compared with a traditional emulsion with the particle size being 3-5 micrometers, and the emulsion is more stable in effect. Compared with the prior art, the weeding composition has the advantages that the pesticide damage risk of 2, 4-dichlorphenoxyacetic acid and fluroxypyr-mepthyl to crops is reduced, the safety of the crops is improved, and meanwhile, the pesticide effect on broadleaf weeds such as scandent hop and field bindweed is also obviously improved. A specific cosolvent is selected, and the prepared missible oil preparation is stable in performance and not prone to layering or crystallization.

The application discloses a traditional Chinese medicine combined medicament for treating 2,4-dichlorophenoxyacetic acid-induced liver injury of livestock and poultry, which is prepared by compounding procyanidin B3, cryptotanshinone and resveratrol, and the three components have synergies and can effectively improve the 2,4-dichlorophenoxyacetic acid-induced liver injury problem of livestock and poultry. The application has simple formula and simple preparation process, can be developed into green and safe liver-protecting veterinary drugs and functional feed, and has good application and popularization value in the field of livestock and poultry breeding.

This invention belongs to the technical field of crystalline materials, and discloses a praseodymium-based complex crystalline material, its preparation method, and its fluorescence recognition application, with the chemical formula {[Pr(L 1.5 The compound is 2,2'-(2,3,5,6-tetramethyl-1,4-phenylene)bis(methylene)bis(sulfonyl)diethyl)benzoic acid. The preparation method involves dissolving Pr(NO3)3·6H2O, H2L, and the auxiliary uncoordinated ligand 1,4-bis(imidazol-1-yl)benzene in a DMF / ethanol / water mixed solvent, reacting at 90°C for 3 days, and then cooling to obtain plate-like crystals. This material is effective against Fe... 3+ It exhibits highly selective and sensitive fluorescence quenching response with a quenching rate of 99.8%; it can specifically recognize ethoxyquinoline (fluorescence enhancement) and 2,4-dichlorophenoxyacetic acid (fluorescence quenching); it can specifically recognize the antibiotic oxytetracycline with a fluorescence quenching rate exceeding 99%.

The invention relates to herbicidal mixtures and compositions comprising isoxaben, dithiopyr, 2,4-Dichlorophenoxyacetic acid (2,4-D), 3,6-dichloro-2-methoxybenzoic acid (dicamba) and methylchlorophenoxypropionic acid (Mecoprop or MCPP), and methods of using the same.

The invention provides a novel dichlorphenoxyacetic acid production process and a device thereof, and relates to the technical field of dichlorphenoxyacetic acid production devices. A neutralizing part is arranged in the fixing part; a middle leakage part is arranged in the fixed part; a dehydration part is arranged in the fixing part; the 2.4-dichlorophenoxyacetic acid sheet solid finished product is obtained by standing for layering, layering the lower layer of 2.4-dichlorophenoxyacetic acid into an inner dewatering cylinder, performing vacuum dewatering, conveying the 2.4-dichlorophenoxyacetic acid to a solid slicing machine through a pipeline, and performing cooling and slicing, so that washing process water is not generated in the production process, drying is not needed, and the production cost is reduced. The invention reduces the production energy consumption of 2, 4-dichlorphenoxyacetic acid, solves the problem of environmental pollution caused by dust generated in the drying process, and solves the problems that a large amount of process water is needed in the production and washing process of 2, 4-dichlorphenoxyacetic acid and steam needs to be consumed in the drying process, so that a large amount of dust is generated in the drying and packaging process, and the production cost of 2, 4-dichlorphenoxyacetic acid is reduced. And environmental pollution is caused.

The invention provides a synergistic weeding composition containing picloram. The synergistic weeding composition comprises an effective component and an auxiliary agent, the effective components comprise 2, 4-dichlorphenoxyacetic acid (2, 4-D) and picloram, and the weight ratio of the 2, 4-dichlorphenoxyacetic acid (2, 4-D) to the picloram is (1: 15)-(18: 1); the auxiliary agent comprises a basic auxiliary agent and a functional auxiliary agent, and the functional auxiliary agent is a combination of a gramineous weed synergist and an anti-drifting agent; the weight percentage of the effective components in the weeding composition is 5%-90%, and the weight percentage of the auxiliary agent in the weeding composition is 10%-95%. According to the scheme, through collaborative screening of the solvent and the auxiliaries, the heat storage (54 + / -2 DEG C, 14 days) layering rate of the soluble concentrate is smaller than or equal to 1%, the suspension rate is larger than or equal to 98%, the stability is remarkably superior to that of an existing compound preparation, and the soluble concentrate is suitable for the uncultivated land complex storage and use environment.

The invention relates to the technical field of plant cultivation, in particular to a top grafting and seed changing method and application thereof. The invention provides a top grafting seed changing method which comprises the following steps: S1, cutting a rootstock to form a grafted point, treating a bud ear with a grafting germination liquid, preserving moisture, and cutting; s2, inserting the cut bud ear in the step S1 into a grafted point of the stock, performing bud ear treatment, and sealing the head of the stock to realize grafting; the grafting germination liquid is prepared from the following components: 6-benzylamino adenine, 2, 4-dichlorphenoxyacetic acid and 1-naphthylacetic acid. According to the top grafting seed changing method, the grafting survival rate and the interface healing speed can be effectively increased, and rapid growth of buds and spikes is promoted; wherein the grafting germination liquid composed of 6-benzylamino adenine, 2, 4-dichlorphenoxyacetic acid and 1-naphthylacetic acid is used for treating and subsequently moisturizing the bud ears, so that the bud ears can be prevented from being dehydrated and dried up and losing the healing meristematic ability, in addition, the joint healing time is also remarkably shortened, and the grafting survival rate is improved.

The invention relates to the technical field of pesticides, and particularly discloses a compound herbicide composition based on 2, 4-D glufosinate-ammonium and a preparation method of the compound herbicide composition. The composition is prepared from raw materials in percentage by mass as follows: 2%-5% of 2, 4-D, 15%-25% of glufosinate-ammonium, 4%-8% of an emulsifier, 0.01%-1% of a defoaming agent, 1%-5% of an anti-freezing agent, 2%-10% of a wetting dispersant, 0.1%-1% of an anti-drifting agent, 1.5%-2.5% of a pH regulator and the balance of water. The invention also provides a preparation method of the composition. Compared with the prior art, the anti-drifting agent is added into the herbicide composition, so that the effects of improving the pesticide effect and reducing the phytotoxicity are achieved.

The application provides a kind of F-free one-dose low IGZO damage metal etching solution and preparation method thereof, and relates to the technical field of semiconductor display panel manufacturing.The F-free one-dose low IGZO damage metal etching solution is composed of the following raw materials: hydrogen peroxide, complexing agent, 2,4-dichlorophenoxyacetic acid, ethylenediaminetetraacetic acid, corrosion inhibitor compound, and ultra-pure water is added to make up the volume;the corrosion inhibitor compound is obtained by mixing 2-mercapto-5-methylbenzimidazole and aminotrimethyl phosphonic acid in a mass ratio of 1:2-3, and the ratio of the addition amount of ethylenediaminetetraacetic acid to the corrosion inhibitor compound is ≤1.2.The application uses a complexing agent-corrosion inhibitor compound synergistic system, the IGZO layer damage rate is <5%, which significantly improves the device yield, and the Cu etching rate is 500-800 Å / min, the MoTi etching rate is 300-500 Å / min, and the rate matching is good.

The present application discloses a kind of herbicidal composition containing 5-phenyl-4,5-dihydroisoxazole-3-carboxylic acid ethyl ester and component B, the component B is selected from one or several of the following: nicosulfuron, sulfometuron, foramsulfuron, fenoxaprop-p-ethyl, benzobicylon, isoxaflutole, dicamba, carfentrazone-ethyl, 2,4-dichlorophenoxyacetic acid and its salt, 2,4-d and its salt, 2,4-d isooctyl and mesosulfuron etc..The composition of the present application has good synergistic effect, is safe to crops, and has broad application prospect.

The invention discloses an organochlorine pesticide visual detection method based on a metal organic framework immobilized enzyme composite material, and belongs to the technical field of biosensors. The method is characterized in that a metal organic framework (MOFs) with a three-dimensional structure is used for encapsulating alkaline phosphatase (ALP) in natural enzyme, and the problems that in a traditional enzyme immobilization technology, bio-enzyme is prone to falling off, and the activity of immobilized enzyme is reduced or lost due to the fact that a solid-phase carrier is broken or dissolved are solved. The MOFs loading capacity is high, so that the target pesticide can effectively inhibit the activity of ALP, the ALP is influenced to hydrolyze p-nitrophenyl phosphonate to generate yellow p-nitrophenol, and an obvious colorimetric signal is generated. According to the present invention, based on the principle, the rapid detection of 2, 4-dichlorophenoxyacetic acid can be achieved, the operation is simple, the cost is low, the sensitivity is high, and the new approach is provided for the rapid detection of 2, 4-dichlorophenoxyacetic acid.

The present application discloses a kind of herbicidal composition containing 5-phenyl-4,5-dihydroisoxazole-3-carboxylic acid ethyl ester and component B, the component B is selected from one or several of the following: nicosulfuron, sulfometuron, foramsulfuron, fenoxaprop-p-ethyl, benzobicylon, isoxaflutole, dicamba, carfentrazone-ethyl, 2,4-dichlorophenoxyacetic acid and its salt, 2,4-d and its salt, 2,4-d isooctyl and mesosulfuron etc..The composition of the present application has good synergistic effect, is safe to crops, and has broad application prospect.

The invention relates to the field of cosmetics, in particular to an astragalus membranaceus callus extract, a preparation method thereof and cosmetics. The method for preparing the astragalus membranaceus callus extract comprises the following steps: culturing under a long-day condition to obtain astragalus membranaceus calluses; the method comprises the following steps: extracting astragalus membranaceus calluses by taking propylene glycol as a solvent; wherein the astragalus membranaceus callus is obtained by inoculating the astragalus membranaceus callus to an induction culture medium for induction, and the induction culture medium comprises 6-benayl aminopurine, naphthylacetic acid and 2, 4-dichlorphenoxyacetic acid; based on the total volume of the induction culture medium, the concentration of the 6-benayl aminopurine is 0.5-2.0 mg / L; the concentration of the naphthylacetic acid is 0.5-2.0 mg / L; the concentration of the 2, 4-dichlorphenoxyacetic acid is 0.1 mg / L to 1.0 mg / L. According to the technical scheme, the content and quality of active ingredients are remarkably improved. The obtained extract has relatively strong antioxidant and whitening activity, is free of other organic solvent residues, is clear in color, and has a relatively good prospect as a cosmetic raw material.

The present application discloses a kind of herbicidal composition containing 5-phenyl-4,5-dihydroisoxazole-3-carboxylic acid ethyl ester and component B, the component B is selected from one or several of the following: nicosulfuron, sulfometuron, foramsulfuron, fenoxaprop-p-ethyl, benzobicylon, isoxaflutole, dicamba, carfentrazone-ethyl, 2,4-dichlorophenoxyacetic acid and its salt, 2,4-d and its salt, 2,4-d isooctyl and mesosulfuron etc..The composition of the present application has good synergistic effect, is safe to crops, and has broad application prospect.

The present invention belongs to the field of biotechnology. More specifically, it relates to a Rosa graciliflora callus cell extract, a preparation method thereof, and uses thereof in the preparation of anti-aging topical skin preparations. The preparation of the Rosa graciliflora callus cell extract includes the steps of first treating the Rosa graciliflora callus with an induction medium added with a low concentration of 2,4-dichlorophenoxyacetic acid and then treating it with an induction medium containing rare earth elements. The Rosa graciliflora callus cell extract prepared by the present invention has a high total polyphenol content, can effectively scavenge free radicals, has the effects of promoting the proliferation of HDF cells and promoting the secretion of COL-I, and can be used in the preparation of anti-aging topical skin preparations.

The present application belongs to the technical field of plant cell culture, and relates to a method for inducing callus of potato 'Kexin 13'. The method comprises the following steps: (1) taking potato 'Kexin 13' explants and transferring them to callus induction medium to form callus, wherein the induction medium is based on MS medium and supplemented with 6-benzylaminopurine, kinetin and 2,4-dichlorophenoxyacetic acid, and has a pH of 5.8-6; (2) taking the callus formed in step (1) and transferring it to differentiation medium to form adventitious buds, wherein the differentiation medium is based on MS basic medium and contains 6-benzylaminopurine, kinetin, gibberellin and myo-inositol, and has a pH of 5.8-6. Through induction culture and differentiation culture, the callus of potato 'Kexin 13' has a high induction rate and differentiation rate.

The present application discloses a kind of herbicidal composition containing 5-phenyl-4,5-dihydroisoxazole-3-carboxylic acid ethyl ester and component B, the component B is selected from one or several of the following: nicosulfuron, sulfometuron, foramsulfuron, fenoxaprop-p-ethyl, benzobicylon, isoxaflutole, dicamba, carfentrazone-ethyl, 2,4-dichlorophenoxyacetic acid and its salt, 2,4-d and its salt, 2,4-d isooctyl and mesosulfuron etc..The composition of the present application has good synergistic effect, is safe to crops, and has broad application prospect.

The present application discloses a kind of herbicidal composition containing 5-phenyl-4,5-dihydroisoxazole-3-carboxylic acid ethyl ester and component B, the component B is selected from one or several of the following: nicosulfuron, sulfometuron, foramsulfuron, fenoxaprop-p-ethyl, benzobicylon, isoxaflutole, dicamba, carfentrazone-ethyl, 2,4-dichlorophenoxyacetic acid and its salt, 2,4-d and its salt, 2,4-d isooctyl and mesosulfuron etc..The composition of the present application has good synergistic effect, is safe to crops, and has broad application prospect.

The invention relates to the technical field of compound production, and discloses a preparation method of a dichlorochrysanthemic acid intermediate 3, 3-dimethyl-4, 6, 6, 6-tetrachloro-hexanoic acid methyl (ethyl) ester, which comprises the following steps: S1, uniformly mixing and heating raw materials carbon tetrachloride and 3, 3-dimethyl-4, 6, 6, 6-tetrachloro-hexanoic acid methyl (ethyl) ester in a reactor to obtain a mixed reaction solution; s2, dissolving a catalyst in an ester organic solvent to obtain a catalyst mixed solution; the catalyst is preferably dibenzoyl peroxide; s3, dropwise adding the catalyst mixed solution into the mixed reaction solution for reaction under the protection of nitrogen; s4, when the conversion rate of the 3, 3-dimethyl-4-pentenoic acid methyl (ethyl) ester is larger than or equal to 98.5%, a free radical trapping agent is dropwise added, after stirring reaction, post-treatment is carried out, and the tetrachloro chrysanthemic acid methyl (ethyl) ester is obtained. According to the scheme, the catalyst is dissolved in the solvent and dropwise added, and the free radical trapping agent is added in the later stage, so that the risk of blocking the free radical chain reaction due to unsafe factors such as overheating or non-uniform distribution of peroxide in the reaction is effectively inhibited, the safety and the reliability are improved, and the industrial large-scale production is more facilitated.

The application discloses a preparation method of 2,4-dichlorophenoxyacetic acid synthesized by composite molecular sieve ZSM-5 and a water-reducing process, and relates to the following steps: under alkaline conditions, 2,4-dichlorophenol is reacted with a sodium chloroacetate solution under the catalysis of a composite molecular sieve catalyst to obtain 2,4-dichlorophenoxyacetic acid sodium salt crude product through condensation; and then hydrolysis and hydrochloric acid acidification are carried out to obtain 2,4-dichlorophenoxyacetic acid product. The process can reduce energy consumption, shorten reaction time and reduce production cost in obtaining 2,4-dichlorophenoxyacetic acid, and can improve the conversion rate and yield of the product and the color of the product. When a new batch of 2,4-dichlorophenoxyacetic acid sodium salt crude product is dissolved in the acidification reaction, the washing water of the last batch of 2,4-dichlorophenoxyacetic acid is used as the solvent reaction liquid, and the washing water of the last batch is used for the first water washing of the final product and is used in turn. The water consumption is reduced, the wastewater treatment cost is reduced, the conversion rate and yield of the product are improved, the product has high purity, the process is simple, the conditions are mild, and the raw materials can be recycled.

The present invention relates to the field of plant cultivation technology, and in particular to a Hainan catalpa test tube seedling, a cultivation method thereof, and a method for cultivating Hainan catalpa seedlings to be transplanted. The present invention includes a Hainan catalpa rooting culture medium, which is based on WPM culture medium and further includes the following components at the following concentrations: 0.1-0.5 mg / L indoleacetic acid, 0.1-0.5 mg / L naphthaleneacetic acid, 0.01-0.05 mg / L 2,4-dichlorophenoxyacetic acid, 15-25 g / L sucrose, and 6.0-8.0 g / L agar; the pH of the Hainan catalpa rooting culture medium is 5.7-5.9. Hainan catalpa test tube seedlings with 5-9 roots can be obtained using the rooting culture medium. The test tube seedlings are transplanted into the seedling culture medium of the present invention, and the transplant survival rate is as high as over 90%. This provides a new approach for the cultivation of Hainan catalpa and the selection of superior varieties.

The invention belongs to the technical field of biology, and particularly relates to an induction culture method of a symphytum officinale genetic transformation system, which comprises the following steps: inoculating a symphytum officinale explant into an induction culture medium containing 0.3-0.5 mg / L naphthylacetic acid and 2-3mg / L 6-benayl aminopurine, and culturing to obtain a callus; inoculating the callus to an adventitious bud induction culture medium containing 0.1 mg / L of 2, 4-dichlorphenoxyacetic acid and 1.0 mg / L of 6-benayl aminopurine for culture, and inducing the callus to differentiate adventitious buds; and inoculating the adventitious buds to an adventitious root induction culture medium containing 0.1 mg / L naphthylacetic acid and 0.5 mg / L 6-benayl aminopurine, and culturing to obtain the symphytum officinale regeneration system. According to the regeneration system establishment method, the symphytum officinale explant infected by the agrobacterium rhizogenes K599 with the GUS reporter gene is cultured, and finally a symphytum officinale regeneration plant with the GUS gene is obtained.