51 results about "Aquaporin" patented technology

The invention relates to an aquaporin bionic nanofiltration membrane and a preparation method and application thereof.The aquaporin bionic nanofiltration membrane is composed of a cell sheet rich in aquaporin and a porous base membrane, and the cell sheet rich in aquaporin serves as a functional unit; through a vacuum filtration method, layer-by-layer assembly and cross-linking curing, the bonding firmness in the active layer is improved. The preparation method disclosed by the invention is simple in operation process, the embedding amount of the aquaporin in the nanofiltration membrane is increased, and the biological activity of the aquaporin is ensured. The aquaporin bionic nanofiltration membrane disclosed by the invention is high in stability and excellent in permeation selectivity, and has a wide application prospect in the field of membrane separation.

The present invention relates to the use of selective aquaporin inhibitors, e.g., of aquaporin-4 or aquaporin-2, e.g., certain phenylbenzamide compounds, for the prophylaxis, treatment and control of aquaporin-mediated conditions, e.g., diseases of water imbalance, for example edema (particularly edema of the brain and spinal cord, e.g., following trauma or ischemic stroke, as well as the edema associated with glioma, meningitis, acute mountain sickness, epileptic seizures, infections, metabolic disorders, hypoxia, water intoxication, hepatic failure, hepatic encephalopathy, diabetic ketoacidosis, abscess, eclampsia, Creutzfeldt-Jakob disease, and lupus cerebritis, as well as edema consequent to microgravity and / or radiation exposure, as well as edema consequent to invasive central nervous system procedures, e.g., neurosurgery, endovascular clot removal, spinal tap, aneurysm repair, or deep brain stimulation, as well as retinal edema), as well as hyponatremia and excess fluid retention, and diseases such as epilepsy, retinal ischemia and other diseases of the eye associated with abnormalities in intraocular pressure and / or tissue hydration, myocardial ischemia, myocardial ischemia / reperfusion injury, myocardial infarction, myocardial hypoxia, congestive heart failure, sepsis, and neuromyelitis optica, as well as migraines, as well as to novel assays for identifying aquaporin inhibitors.

The invention discloses bifidobacterium longum subsp. Infantis for regulating intestinal motility and aquaporin and application of the bifidobacterium longum subsp. Infantis, and belongs to the technical field of microorganisms. According to the invention, the bifidobacterium longum subsp. Infantis CCFM1269 is screened, and the bifidobacterium longum subsp. Infantis CCFM1269 has the effects of intestinal motility and aquaporin, specifically, the defecation frequency is reduced, and the water content of excrement and the small intestine propulsion rate are reduced. The intestinal motility abnormality is relieved by reducing the levels of neurotransmitters 5-HT, SP and VIP, and the intestinal moisture absorption and secretion are regulated by improving the mRNA expression of aquaporin AQP4 and AQP8. Meanwhile, the intestinal flora is adjusted by improving the diversity of the intestinal flora, and the content of propionic acid and butyric acid in excrement is increased.

This application relates to a pharmaceutical composition of an aquaporin inhibitor and a method for producing the same. The pharmaceutical composition comprises 2-((3,5-bis(trifluoromethyl)phenyl)carbamoyl)-4-chlorophenyl dihydrogen phosphate or a pharmaceutically acceptable salt thereof, or a pharmaceutically acceptable solvate thereof, and meglumine. The pharmaceutical composition of an aquaporin inhibitor and a method for producing the same have the following advantages: the process is simple, easy to operate, conducive to industrial production, good product stability, and a significantly reduced content of degradable impurities, which ensures pharmaceutical efficacy.

The present invention discloses the application of a small molecule aquaporin inhibitor in resistance to silkworm pebiosis. The present invention adopts molecular docking technology to screen a small molecule database and performs activity detection on the obtained small molecule inhibitors, thereby obtaining a small molecule aquaporin inhibitor with resistance to silkworm pebiosis. The present invention uses the screened small molecule aquaporin inhibitor as a drug for preventing and treating silkworm pebiosis, thus developing a new application of the inhibitor. When the final concentration of the aquaporin inhibitor is 0.5 μM or more, the germination of silkworm microsporidia in vitro can be inhibited; when the final concentration is 1 μM or more, the proliferation of silkworm microsporidia in silkworm cells can be reduced; and when the final concentration is 100 μM or more when added to feed, the incidence of silkworm pebiosis can be reduced.

The present invention relates to the therapeutic use of non-classical human major histocompatibility complex (MHC) molecules (also known as MHC class Ib molecules) in combination with a peptide antigen for the treatment of neuromyelitis optica (NMO). More specifically, the present invention relates to recombinant polypeptides comprising a peptide antigen in combination with one or more domains of a non-classical MHC class Ib molecule. The present invention also relates to methods of producing such recombinant polypeptides, pharmaceutical compositions comprising such recombinant polypeptides, and their use in the treatment of neuromyelitis optica (NMO).

To provide a compound which can be used for prevention, treatment and control of aquaporin-mediated conditions such as diseases of water imbalance, for example, cerebral edema, a pharmaceutical composition, and a method for producing the same.SOLUTION: There is provided a hydrate of 2 - {[3, 5-bis (trifluoromethyl) phenyl] carbamoyl} - 4-chlorophenyl dihydrogen phosphate represented by the following structure, which exhibits an XRPD pattern comprising at least five 2 θ (°) values selected from the group consisting of 8.8, 9.5, 11.1, 15.2, 15.5, 16.4, 20.2, 20.6, 23.6, 24.0, 24.9 and 27.2, wherein the XRPD is measured using an incident beam of CuK α radiation of 1.54059 Å.SELECTED DRAWING: None

The invention discloses a dampness-clearing sleep-aiding Tibetan foot bathing tablet and a preparation method thereof, and relates to the technical field of traditional Chinese medicine preparations, the dampness-clearing sleep-aiding Tibetan foot bathing tablet is composed of a monarch drug group, a ministerial drug group, an adjuvant drug group, a conductant drug group and an auxiliary material group, and the preparation method comprises the following steps: step 1, crushing; step 2, weighing; step 3, extracting active ingredients; step 4, drying and powdering; step 5, total mixing; step 6, tabletting; step 7, inner packaging; and step 8, external packaging. Through the compatibility of monarch, minister, assistant and guide, the synergistic effect of dampness clearing and sleep aiding is realized, the monarch drug group and the adjuvant drug group synergistically regulate a dampness clearing pathway (AQP4 aquaporin + TRPV1 ion channel), the minister drug group and the guide drug group synergistically enhance the sleep aiding effect (GABA receptor activation + glutamic acid inhibition), and clinical experiments show that after the traditional Chinese medicine composition is used for 4 weeks, the effective rate of improving the dampness symptom reaches 80%, and the curative effect is good. And the sleep quality improvement rate reaches 82%.

The application belongs to the technical field of microorganisms, and provides application of Propionibacterium freudenreichii subsp. shermanii in preparation of products for improving constipation and depression, wherein the Propionibacterium freudenreichii subsp. shermanii NHNK-616 is preserved in the China Center for Type Culture Collection on July 1, 2024, and the preservation number is CCTCC NO: M 20241429. The inventors find that NHNK-616 has the functions of promoting 5-HT secretion, up-regulating intestinal aquaporin and brain-derived neurotrophic factor genes, metabolically producing gamma-aminobutyric acid, inhibiting growth of Candida albicans, inhibiting hypha formation of Candida albicans, combining with hypha of Candida albicans, and up-regulating expression of intestinal defensin and mucin genes, and therefore can be used for preparing products for improving constipation and depression.

The invention belongs to the technical field of probiotics, and particularly relates to lactobacillus plantarum MB02 with moisturizing and tightening effects and application of the lactobacillus plantarum MB02. According to the invention, a new lactobacillus plantarum MB02 strain is separated from feces of a healthy infant in Guangzhou city, Guangdong province, a zebra fish dehydration model is established through a sodium chloride hypertonic solution, and research finds that the lactobacillus plantarum MB02 strain can realize moisturizing and tightening effects through regulation and control of various genes and material synthesis; comprising the following steps: obviously improving the expression quantity of collagen genes col1a1a and col1a1b, an elastin gene eln1, a hyaluronic acid synthetase gene has3 and an aquaporin gene aqp3; the contents of hyaluronic acid and equol are obviously increased. Therefore, the lactobacillus plantarum MB02 strain has high application value in the field of cosmetics and can be used for developing products for moisturizing, resisting wrinkles and tightening skin.

The invention relates to a bacteriostatic repair medical gel containing glycosylglycerol and a preparation method thereof, and belongs to the technical field of medical dressings. The gel is prepared by taking glycosylglycerol as a main active component and cooperating with chitosan, collagen, a silver ion antibacterial agent and other components through a specific process. The gel has triple synergistic effects: glycosylglycerol enhances the skin barrier function and provides lasting moisture retention by activating aquaporin; chitosan and silver ions provide broad-spectrum antibacterial activity; and collagen and glucan promote tissue repair and cell proliferation. Experiments show that the antibacterial rate of the gel on common pathogenic bacteria such as staphylococcus aureus and escherichia coli exceeds 99.9%, meanwhile, the wound healing speed can be increased by 40% or above, and the gel is good in biocompatibility and free of skin irritation. The gel can be used for repairing and nursing various skin injuries such as burn wounds, chronic ulcers and surgical wounds.

The application belongs to the technical field of microorganisms, and discloses a propionic acid-producing propionic acid bacteria NHNK-621 and application of the propionic acid bacteria in preparation of products for improving constipation and memory, the bacteria strain has been preserved in the China Center for Type Culture Collection on November 25, 2024, and the number is CCTCC NO: M 20242631. The propionic acid bacteria NHNK-621 provided by the application has the functions of promoting 5-HT secretion, up-regulating intestinal aquaporin and brain-derived neurotrophic factor genes, metabolically producing gamma-aminobutyric acid, inhibiting growth of Candida albicans, inhibiting hypha formation of Candida albicans, combining with hypha of Candida albicans, up-regulating intestinal defensin and mucin gene expression, in addition, the bacterial biofilm can increase the colonization ability to intestinal epithelial cells, and can be used for preparing products for improving constipation and memory.

The application discloses a two-compartment modeling and prediction method for sodium kinetics in hemodialysis. The method divides the patient's body fluid into two compartments of extracellular fluid and intracellular fluid, and establishes a coupled differential equation set to describe the sodium transfer kinetics in the dialysis process. The application corrects the physical error of the existing model in the dispersion equilibrium concentration formula, proposes C_eq=[Na]d / σ (σ=0.97 is the Gibbs-Donnan factor) to replace the traditional Donnan formula and the old recommended formula, eliminates the false dispersion gradient under the condition of isonatric dialysis based on the physical fact that electrolytes are only distributed in the plasma water phase, and removes the error solvent drag term by clearly defining the biophysical constraints of the cell membrane aquaporin not transporting sodium. The application adopts a perturbation reference model to replace the traditional Fick diffusion law to describe the cell membrane sodium exchange, and more accurately reflects the intracellular and extracellular sodium kinetics. The model can accurately predict the serum sodium change and sodium removal under different dialysis prescriptions, and can generate a sodium removal contour map for clinical prescription optimization.

The invention discloses application of leuconostoc mesenteroides subsp. Mesenteroides in improvement of constipation and depression, and belongs to the technical field of marine microorganisms. The invention discloses a leuconostoc mesenteroides subsp. Mesenteroides NHNK-613, which is preserved in the China Center for Type Culture Collection on July 1, 2024, and the preservation number of the leuconostoc mesenteroides subsp. Mesenteroides NHNK-613 is CCTCC (China Center for Type Culture Collection) NO: M 20241431. The NHNK-613 has the functions of promoting 5-HT secretion, up-regulating intestinal aquaporin and brain-derived neurotrophic factor genes, metabolizing to generate gamma-aminobutyric acid, inhibiting the growth of candida albicans, inhibiting the formation of candida albicans filaments, combining the candida albicans filaments and up-regulating intestinal defensin and mucoprotein gene expression; the traditional Chinese medicine composition can be used for preparing products for improving constipation and depression.

The invention relates to the technical field of beauty skin care products, in particular to a glutamic acid derivative, a preparation method and application of the glutamic acid derivative in skin care products. The invention provides a compound with a structure as shown in a formula (I), which not only has rhythm protein up-regulation expression and oxidation resistance, but also has a good effect on up-regulation of aquaporin expression, silk protein expression and hypoxia inducible factor expression. The potential application prospects are realized on the effects of moisturizing the skin, repairing the barrier, promoting the ruddy skin and the like.

The invention discloses a germplasm aquaporin OsPIP2 and a preparation method thereof. 1 is applied to regulation and control of rice chalkiness, and belongs to the technical field of biological breeding. According to the invention, a gene OsPIP2 for controlling chalkiness of rice is cloned; 1, the nucleotide sequence of the gene is as shown in SEQ ID NO. 1. According to the invention, a gene editing technology Crispr / cas9 is used for modifying OsPIP2 in wild type ZH11; after the function of the rice chalkiness gene OsPIP2 is destroyed, knockout strains show that the chalkiness rate and the chalkiness degree of rice grains are remarkably improved, which indicates that the gene has the function of regulating and controlling rice chalkiness, and meanwhile, analysis finds that the rice chalkiness gene OsPIP2 is a japonica rice type OsPIP2; the chalkiness of 1Hap3 is obviously lower than that of other haplotypes, which indicates that OsPIP2; the 1Hap3 has the potential of reducing chalkiness of indica rice, gene resources can be provided for high-quality breeding of rice, and reference is provided for functional research of other homologous genes.

The invention belongs to the field of crop genetic breeding, and relates to an application and a method of a sorghum aquaporin PIP1-5 gene in cultivation of a transgenic plant, the aquaporin gene is cloned from a drought-enduring crop sorghum, the aquaporin gene is named as PIP1-5, and transgenic yeast and transgenic arabidopsis experiments prove that the gene plays a role in drought tolerance. In addition, drought stress experiments and physiological index detection prove that the oilseed rape transfected with the gene has good drought tolerance.

This invention belongs to the field of biomedical technology and relates to a composition based on plant essential oils or active ingredients and its application in the preparation of drugs for the prevention and treatment of pulmonary edema. It comprises at least three plant essential oils or active ingredients; said plant essential oils or active ingredients have agonistic activity against aquaporins and agonistic activity against sodium-potassium ATPase. The composition and its special formulation provided by this invention not only have good preventive and therapeutic effects on pulmonary edema, but can also be applied to the prevention and treatment of related lung diseases such as hypersputum secretion, acute lung injury, acute respiratory distress syndrome, pulmonary fibrosis, and COPD.

The invention relates to the technical field of biological medicine, in particular to an anti-tumor nanoparticle and application. The invention provides a brand-new immunogen cell death mode and a strategy for constructing an in-situ whole tumor cell vaccine. The encoding nucleic acid of the aquaporin is supported by using LNP or a polymer carrier, so that the encoding nucleic acid is expressed in tumor cells, osmotic pressure difference is regulated and controlled to enable a large amount of water molecules to enter in a short time, tumor cell membranes are expanded and broken, and immunogenic death is initiated. In the process, tumor cells release immunocompetent substances in situ to induce efficient immune response, and experiments show that the vaccine prepared by the invention inhibits the growth of mouse melanoma and breast cancer.

The invention discloses bifidobacterium breve for regulating intestinal motility and aquaporin and application of the bifidobacterium breve, and belongs to the technical field of microorganisms. According to the invention, a strain of bifidobacterium breve CCFM1078 is screened out, and the bifidobacterium breve CCFM1078 has the effect of regulating intestinal motility and aquaporin, specifically, the defecation frequency is reduced, and the water content of excrement and the small intestine propulsion rate are reduced. The intestinal motility abnormality is relieved by reducing the levels of neurotransmitters 5-HT, SP and VIP, and the intestinal moisture absorption and secretion are regulated by improving the mRNA expression of aquaporin AQP3, AQP4 and AQP8. Meanwhile, the intestinal flora is adjusted by improving the diversity of the intestinal flora, and the content of propionic acid and butyric acid in excrement is increased.

The invention discloses a lactobacillus rhamnosus metagen with a constipation relieving effect and application of the lactobacillus rhamnosus metagen, and belongs to the field of microorganisms. The lactobacillus rhamnosus CCFM1463 metagen provided by the invention can significantly reduce the inflammation level in colon tissue, improve pathological damage of colon tissue, reduce the expression level of proinflammatory factors, improve the expression level of anti-inflammatory factors, reduce the expression level of aquaporin, and increase the protein content of motilin (MTL), vasoactive intestinal peptide (VIP) and substance P (SP) in serum; the compound has the advantages of enhancing intestinal tract movement, shortening intestinal tract transfer time and regulating intestinal tract steady state, and has a huge application prospect in preparation of medicines for relieving constipation.

The invention provides a collagen peptide-containing sebum membrane repairing composition and a preparation method thereof, and relates to the field of biomedical materials. The sebum film repairing composition is prepared from a recombinant III type collagen peptide, a recombinant I type collagen peptide, a ceramide compound, squalane, a natural moisturizing factor, glycosyl trehalose, phytosterol, glycerol, butanediol, polyglycerol-10 laurate, laurocapram and water. According to the sebum membrane repairing composition, the recombinant III-type collagen peptide and the recombinant I-type collagen peptide have a synergistic effect with the ceramide compound, so that the ceramide recovery rate is greatly improved, and the composition can realize'lipid barrier-aquaporin-tight connection 'three-in-one repairing of a sebum membrane.

The invention discloses a vacuolar aquaporin OsTIP1 and a preparation method of the vacuolar aquaporin OsTIP1. The invention discloses application of 2 in regulation and control of rice quality and belongs to the technical field of biological breeding. According to the invention, a vacuolar aquaporin family gene OsTIP1 for controlling rice quality is cloned; 2, the nucleotide sequence of the gene is as shown in SEQ ID NO. 1. According to the invention, a CRISPR / Cas9 gene editing technology is used for editing the gene OsTIP1 in wild type rice; after OsTIP1 and OsTIP2 are knocked out, the chalky grain rate of transgenic line grains is remarkably increased compared with that of wild type grains, it is indicated that the gene participates in and regulates chalky formation and has the function of regulating the rice quality, and meanwhile it is found through analysis that OsTIP1; the chalkiness of 2Hap4 is obviously lower than that of other haplotypes, and the gene can be used for breeding high-quality varieties of cereal crops such as rice and the like, and also provides reference for cloning and research of homologous genes in other species.

The invention discloses a skin recuperating method for regulating aquaporin based on multi-waveform medium-frequency electric signals, and relates to the technical field of skin physical therapy, which comprises the following steps: (a) symmetrically attaching a pair of annular medical zinc alloy electrodes to a skin treatment area as an anode and a cathode respectively; (b) starting a preset treatment program through a program control module, and outputting intermediate-frequency current with the frequency range of 1kHz-10kHz to the electrode; (c) dynamically switching or superposing the intermediate-frequency current to output at least two waveforms of sine waves, sharp waves and square waves; (d) up-regulating the expression of aquaporin AQP3 in keratinocytes by utilizing the synergistic effect of zinc ions released by the zinc alloy electrode and multi-waveform electrical stimulation; ions continuously released by the zinc electrode permeate into an epidermal substrate layer, metal response element binding factors are activated, and the transcriptional activity of skin moisturizing and barrier repairing genes is enhanced.

The invention discloses a polysaccharide composition and application thereof, and relates to the technical field of polysaccharide preparation, the polysaccharide composition comprises at least one of a first polysaccharide, a second polysaccharide, a third polysaccharide and a fourth polysaccharide, the structural formulas of the first polysaccharide, the second polysaccharide, the third polysaccharide and the fourth polysaccharide are respectively shown as a formula (I), a formula (II), a formula (III) and a formula (IV), and the molecular weights of the polypeptide are respectively 343016.9 Da, 2279.4 Da, 1363.2 Da and 2228.9 Da. In the polysaccharide composition provided by the invention, the four polysaccharides have better antioxidant activity, can better inhibit alpha-glucosidase, alpha-amylase and pancreatic lipase, and can be strongly combined with AQP1 aquaporin, so that the four polysaccharides can be used for preparing antioxidants, hypoglycemic drugs, hypolipidemic drugs and drugs for treating AQP1 mediated diseases.

The present invention relates to delivering benefits related to maintaining a resilient microbiome on skin through a personal care composition, especially through wash off compositions like soap, body wash or shampoo. This is achieved by using starch or its microbial metabolite preferably in such compositions. It works by upregulating expression levels of Aquaporin-3, Claudin 1, Keratin- 4, Filaggrin- 1 or Desmoglein 1 on skin.

The invention relates to a novel application of small extracellular vesicles with high expression of NAMPT in preparation of drugs for relieving or treating traumatic brain injury. The invention finds that the NAMPT-sEV administration in the nose can obviously increase the expression of Sirtuin 1 of an rmTBI patient, so that tau is deacetylated. Besides, the NAMPT-sEV inhibits neuroinflammation, maintains the polarity of aquapoprotein-4, promotes the repair of a lymphatic system and a meningeal lymphatic system, and is beneficial to the removal of ac-tau protein in brain tissues, and the reduction of the ac-tau protein can prevent the degeneration of an initial axon segment and the wrong positioning of the tau protein. In conclusion, the invention provides that NAMPT-sEV reduces neuron loss of rmTBI and improves cognitive function through various mechanisms, and the small extracellular vesicles highly expressing NAMP can be well used for alleviating or treating traumatic brain injury and dementia including Alzheimer's disease.

The invention provides a method for synergistically regulating cotton fiber elongation and verticillium wilt resistance based on an upland cotton GhMP gene and a transgenic cotton plant, and relates to the technical field of genetic engineering. The gene encodes a membrane protein, and overexpression and RNA interference transgenic line experiments prove that the expression level of GhMP is positively correlated with fiber development and disease resistance: the fiber length can be significantly increased and the verticillium wilt resistance can be improved by expression increase, and the fiber shortening and the resistance weakening can be caused by expression reduction. A further research shows that the GhMP protein is positioned on a cell membrane, can interact with GhPIP2.7 and GhTIP1.1 aquaporin, and jointly participates in hydrogen peroxide transmembrane transport to form a molecular compound for regulating and controlling fiber elongation and preventing diseases. The invention provides a key target gene with both quality improvement and disease resistance enhancement, provides a new strategy and gene resources for cotton multi-trait molecular breeding, and has important application value and popularization prospect.

The present disclosure relates to a cosmetic composition containing an extract of Cyperus rotundus extracted using warm microbubbles. The extract inhibits nitric oxide (NO) production and exhibits excellent anti-inflammatory efficacy by inhibiting the expression of interleukin 6 (IL-6), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α), which are inflammatory expression factors. In addition, the extract enhances the expression of aquaporin-3 (AQP-3), hyaluronan synthase 2 (HAS-2), claudin-1 (CLDN-1), cadherin 1 (CDH1), filaggrin (FLG), and involucrin (IVL), which are genes related to the skin barrier and moisturizing. Thus, when using the extract for cosmetic products, excellent effects can be exhibited in skin soothing, moisturizing, enhancement of the skin barrier, relief of irritation such as erythema, relief of pruritus, and the like, so the extract can be usefully applied to multi-care products that improve skin conditions.