175 results about "Aquaporin" patented technology

Disclosed are novel water membranes comprising lipid bilayers incorporating functional aqua-porins. The lipid bilayers are arranged in sandwich structures including hydrophilic or hydro-phobic support materials. Also disclosed are water purification devices / systems, including reverse osmosis filtering devices that include membranes having functional aquaporins. Methods of water purification and methods of preparing the membranes are also disclosed. Further, the invention provides for a new type of perforated, hydrophobic polymer film and to membranes containing lipid bilayers having other transmembrane proteins than aquaporins introduced.

The present invention provides methods for producing salinity power using pressure retarded osmosis and a biometric membrane (e.g., a liquid bilayer membrane or a lipid membrane containing multiple bilayers of fused deposited lipid vesicles) containing aquaporin water channels. The invention also provides power plants for producing salinity energy using pressure retarded osmosis and a biometric water membrane containing functional aquaporin channels.

A liquid membrane matrix is disclosed in the form of an aquaporin containing bulk liquid membrane matrix (BLM), wherein said liquid membrane matrix is formed from a solution of an amphiphilic copolymer detergent wherein transmembrane proteins have been functionally incorporated and wherein said matrix further contains a stabilising oil phase. The uses of the membrane matrix include water extraction from liquid aqueous media by forward osmosis, e.g. for desalination of salt water.

The present invention discloses a water membrane comprising a lipid bilayer supported on a single side thereof on a water permeable dense support layer, this lipid bilayer being composed of one or more lipids and aquaporin proteins are embedded therein, further wherein the water permeable dense support layer is impermeable to the lipids and to the aquaporin proteins. Also are provided a method for the preparation of these membranes and uses thereof in water filtration applications.

The present invention relates to the use of aquaporin containing membranes in the production of salinity power. The membranes are preferably lipid bilayers incorporating reconstituted aquaporin channels, e.g. entrapped between porous support materials, or alternatively enclosing a highly hydrophobic porous support material. Also disclosed is a salinity power plant.

A portable Static Electro Magnetic Field (SEMF) generating device and method for the therapeutic treatment of inflammatory and painful disorders of the human limbs including fingers, hands, wrists, forearms, elbows, toes, feet and ankles preferably includes a wearable, portable battery powered solenoid or coil. The coil is configured to focus a substantially time invariant magnetic flux field onto a selected part of the body. The SEMF generating device's coil, when energized, works by slightly changing the charges associated with voltage-dependant ion channels in the cell membranes of the human body, thereby stabilizing abnormal fluid transport into the cells through aquaporin channels and the sodium ions relating to them, treating inflammation and altering the flow of calcium, chloride and potassium ions through their respective neuronal ion channels and treating pain.

The invention particularly discloses a composition for promoting opening of skin aquaporin, which is mainly proportionally prepared from deionized water, glycerol, carbomer, xanthan gum, lycine, trehalose, inositol, taurine, lauryl mammary gland sodium lactate, monomethyl-monosilane triol, oxyproline, aspartic acid, yeast amino acid, polyglutamic acid, sodium hyaluronate, dipotassium glycyrrhetate, purslane extracting solution, licorice extract, ceramide, phytosphingosine, cholesterol, oligopeptide-1, polyquaternary amine salt-14 and the like. The composition has the function of promoting opening of skin aquaporin, has better water replenishing and locking effect, and effectively prevents and resists the phenomena of dry skin mucosa and skin aging.

The present invention relates to a water extraction system comprising a flow cell comprising a membrane; said membrane comprising an active layer comprising immobilized aquaporin water channels and a support layer, and said membrane having a feed side and a non-feed side; and an aqueous source solution in fluid communication with the feed side of the membrane.

The invention discloses a reverse osmosis membrane containing aquaporins and a preparation method of the reverse osmosis membrane. According to the preparation method of the reverse osmosis membrane, modification is carried out on the surface of a polysulfone support membrane, an amino functional group is grafted, amino participates into interfacial polymerization reaction of polyamide, and connection between a polysulfone support layer and a polyamide active layer is acting force of a chemical bond, so that the binding force between the polysulfone support layer and the polyamide active layer is strengthened to effectively prolong the service life of the composite reverse osmosis membrane.

The invention provides a preparation method of a compound reverse-osmosis membrane. The method includes the steps of sequentially coating a supporting layer of a substrate with a polyamine water solution, a vesica solution containing aquaporins, and a polyacyl chloride organic solution, and conducting post-treatment after interfacial polymerization reaction to obtain the compound reverse-osmosis membrane. The polymer porous supporting layer where polyamine is adsorbed is coated with the vesica solution containing aquaporins, and interfacial polymerization is conducted on the supporting layer and the polyacyl chloride organic solution to obtain the compound reverse-osmosis membrane. It is shown through experiment results that the prepared compound reverse-osmosis membrane has a desalinization ratio of 99.3% or above at the temperature of 25 DEG C, the pressure of 5 bar and the water flux of 15 LMH / bar(45GFD) or above under the testing condition of a water solution with 500 ppm of sodium chloride and pH of 6.5.

The invention discloses a detection method of an aquaporin-4 autoantibody, a fusion expression virus vector and application thereof. The virus vector is pCDH-MCS-AQP4-EF1-copGFP, the nucleotide sequence of which is shown as a sequence table SEQIDNO.2. The invention further discloses application of the fusion expression virus vector in improving the clinical diagnosis and treatment level of ophthalmoneuromyelitis and multiple sclerosis. By adopting immunofluorescent cytochemical staining with higher sensitivity and specificity combined with a flow cytometric detection technology, the AQP-4 antibody is quantitively and qualitatively detection clinically, so that the detection result is more stable, and the labor and the time cost are extremely saved. The project is established to extremely improve the diagnosis and treatment level of ophthalmoneuromyelitis and multiple sclerosis, so that the labor capacity and the living quality of the patient are improved, and huge social and economic benefits are generated.

The invention relates to a preparation method of a phospholipid biomimic membrane containing aquaporins. A composite membrane the surface of which is electronegative is prepared by layer-by-layer assembly, and a phospholipid vesicle solution with electropositivity, which is wrapped with aquaporins, spreads on a composite membrane by an electrostatic bonding effect so as to form a phospholipid dual-layer membrane. According to the invention, the phospholipid biomimic membrane containing aquaporins is prepared by layer-by-layer assembly, after aquaporins is added, the flux of the membrane is improved by 4-5 times compared with the original membrane, and is about 16-28L / m2h (the water flux of the original membrane is 3-5L / m2h); the reject rate of monovalence Na<+1> is improved by more than 40%; the reject rate of bivalence Mg<+2> is improved by more than 65%; and the preparation method of the phospholipid biomimic membrane containing aquaporins can be applied to different fields of water softening, wastewater treatment and the like, and has a wide application prospect.

Provided herein are methods for identifying and characterizing agents that alter the volume of a cell. Methods are provided for rapid screening and identification of an agent that alters the capability of a small, neutrally charged solute transporter to transport the solute across a cell membrane. The methods described herein may be used to identify and characterize inhibitors of urea transporters, to identify and characterize inhibitors of aquaporins, and to identify and characterize inhibitors of other small, neutrally charged solutes such as glucose.

Compounds, including 3-carboxy aryl sulfonamide compounds, which agonize or antagonize aquaporin channels and methods of using them to treat disorders or diseases mediated by aquaporins.

Present invention relates to a thin film composite membrane wherein a thin selective layer, having incorporated amphiphilic vesicles, is supported by a microporous substrate. A process of preparing the thin film composite membrane and its use are also disclosed.

The invention relates to research field of plant stress resistance gene. The invention provides a peanut aquaporin gene AhAQ1 gene order and a coded protein order thereof. The invention also providesa method for cloning the aquaporin gene AhAQ1 in a peanut. The expression analysis of the aquaporin gene AhAQ1 shows that the expression of the gene is obviously increased in different tissues of thepeanut under the condition of salt stress and also shows that the gene is related to the plant salt resistance. The transgene test analysis also shows that the overexpression of the gene can enhance the salt resistance of the peanut to a certain extent.

The invention discloses a moisturizing combination containing cladosiphon novae-caledoniae extract and a preparation method thereof, relates to the technical field of skin care products, and solves atechnical problem of poor moisturizing effects of existing moisturizing products. The moisturizing combination is characterized by comprising an A component and a B component, the A component is prepared from the following ingredients in parts by weight: 2.12 to 13 parts of sodium hyaluronate mixture, 2 to 7 parts of saccharide isomer, 6 to 15 parts of alcohol humectant, 0.01 to 0.2 part of ethylhexyl glycerol, 15 to 25 parts of deionized water; the B component is prepared from the following ingredients in parts by weight: 20 to 30 parts of cladosiphon novae-caledoniae extract and 10 to 20 parts of 1,3-propylene glycol. The aquaporin expression can be promoted by adopting sodium hyaluronate to supplement and store water, and compounding of saccharide isomer and polyquaternium-51, and the cladosiphon novae-caledoniae extract forms a dense invisible protective film, and the moisturizing combination containing the cladosiphon novae-caledoniae extract has the advantages of supplementing water in an outer layer, locking water in an inner layer, moisturizing layer by layer, and moisturizing and lightening skin from the inside to out.

Compounds, including 3-carboxy aryl sulfonamide compounds, which agonize or antagonize aquaporin channels and methods of using them to treat disorders or diseases mediated by aquaporins.

The invention relates to a preparation method for a double-skin forward permeable membrane with aquaporin. The preparation method specifically comprises the following steps of: 1) preparing a base membrane; 2) preparing a layer-by-layer assembly composite membrane; 3) preparing phospholipid vesicle solution; 4) introducing aquaporin; and 5) preparing a biomimic film with aquaporin phospholipid, wherein proteoliposome with aquaporin prepared in the step 4 is spread to the surface of the composite film prepared in the step 2, and then a cross-linking agent is used to carry out cross-linking to complete the preparation. According to the preparation method, the double-skin forward permeable membrane with aquaporin is prepared by layer-by-layer assembly; the double-skin forward permeable membrane can effectively prevent solute from entering the composite membrane, thereby reducing inner concentration polarization effect, and obviously improving effective water flux of the forward permeable membrane; and the the double-skin forward permeable membrane can be applied to different fields such as water treatment, membrane separation and the like, and has a wide application prospect.

The invention belongs to the field of cosmetics, and relates to a moisturizing technology in skin care products, namely a 24-hour moist three-layer moisturizing and repairing system. The skin care product provided by the invention is characterized in that kelp extractive, deep ocean water, pansy extractive and a water catching factor form a three-layer moisturizing and repairing system; the kelp extractive contains a plurality of minerals, saccharides and amino acid and has the moisturizing effect of reinforcing surface water locking of skin; the pansy extractive promotes synthesis of aquaporins in cell membranes, improves water circulation of dermis and promotes water to transfer from dermis to cuticle; silicon and manganese elements in the deep ocean water can reduce transepidermal water loss and are cooperative to achieve the effect of activating water deeply; and the water catching factor ensures that skin has the moisturizing effect of catching water outside. By means of the three-layer moisturizing and repairing system including surface water locking, water activating deeply and water catching outside, the skin care product provided by the invention can bring an intelligent lasting moisturizing technology to skin, so that bright, smooth, transparent and moist skin is brought.

The present invention relates to a hollow fiber (HF) module having fibers modified with a thin film composite (TFC) layer comprising aquaporin water channels.

The invention relates to a dendrobium-extract-containing composition, and the dendrobium-extract-containing composition can improve the problems of skin roughness, dryness and the like caused by skin water shortage, and can effectively resist oxidation and prevent aging. The dendrobium-extract-containing composition comprises the following components: a dendrobium extract, hydrogenated lecithin and plant sterol. The dendrobium extract is prepared by solvent extraction, and a solvent is selected from the group consisting of water, methanol, ethanol, isopropanol, n-butanol, ethylene glycol, 1,2-propylene glycol, 1,3-propylene glycol, 1,3-butanediol, acetone, ethyl acetate, or a combination thereof. The dendrobium-extract-containing composition can well promote expression of keratinocyte AQP3 (3th aquaporin) and enhance the penetration capability of cells, and at the same time has good water locking and moisturizing effects due to the synergistic effect of the various components. The invention also includes a preparation method of the composition, the preparation method has the advantages of simple process and low cost, and a produced product has excellent quality.

The invention provides an original ferment straining and repairing mask. The mask comprises the following raw materials: butylene glycol, glycerin, tremella sporophore extract, a yeast fermentation product, centella extract, ginseng extract, large-flowered skullcap root extract, sodium hyaluronate, rose flower extract, betaine, bio-saccharide gum-1, licorice root extract and peony root extract. The original ferment straining and repairing mask supplies facial skin with nutrients, dredges aquaporin and nutrient absorption channels in time and has doubled skin-care effect. The invention also provides a preparation method for the original ferment straining and repairing mask.

The invention discloses a manufacturing method of a composite membrane containing aquaporin. The manufacturing method comprises the steps that dopamine is auto-polymerized on the surface of a base membrane, and the base membrane with polymerized dopamine attached to the surface and amidogen of a phosphatidyl ethanolamine membrane containing aquaporin are made to react, and therefore the composite membrane is manufactured. According to the manufactured composite membrane, covalent bonds exist between a functional layer and the base membrane, composite strength is high, etching resistance and hydrolysis resistance are strong, service life is long, surface hydrophilicity is good water permeability is high, the manufacturing condition is mild, and the composite membrane has wide application prospects in the field of water treatment and membrane separation.

The invention discloses a skin barrier repair bionic composition and a preparation method thereof, the composition comprises the following components by mass: 7.5-10% of a humectant, 7-10% of an emollient, 0.4-0.5% of a thickener, 2.5-4.5% of a liquid crystal bionic emulsifier, 11-18% of a skin barrier repair agent, a proper amount of a pH regulator, and the balance of water, and the sum of the mass percentages of the components is 100%. The skin barrier is comprehensively repaired from five dimensions of supplementing a sebum membrane, repairing a brick wall structure, adjusting aquaporin expression, supplementing natural moisturizing factors, inhibiting inflammation, strengthening an immune system, promoting dermal layer fibroblast proliferation and protecting collagen and a matrix, andmeanwhile, a liquid crystal bionic and bionic phospholipid emulsification process is adopted, so that the skin barrier is repaired. The skin barrier repairing bionic composition provided by the invention has the effects of efficiently repairing a skin barrier for a long time, resisting inflammation, maintaining stability and strengthening skin, and is comfortable and safe.

This invention relates to methods for increasing carbon fixation and / or increasing biomass production in a plant, comprising: introducing into a plant, plant part, and / or plant cell one or more heterologous polynucleotides encoding polypeptides having the enzyme activity of succinyl CoA synthetase, 2-oxoglutarate:ferredoxin oxidoreductase, 2-oxoglutarate carboxylase, oxalosuccinate reductase and isocitrate lyase to produce a stably transformed plant, plant part, and / or plant cell expressing the one or more heterologous polynucleotides. The methods further comprise introducing into a plant, plant part or plant cell heterologous polynucleotides encoding polypeptides having the enzyme activity of glyoxylate carboligase and tartronic semialdehyde reductase, and / or heterologous polynucleotides encoding a superoxide reductase from an archaeon species, an aquaporin and / or an inhibitor of cell wall invertase inhibitor. Additionally, transformed plants, plant parts, and / or plant cells are provided as well as products produced from the transformed plants, plant parts, and / or plant cells.

The present invention relates to a water extraction system comprising a flow cell comprising a membrane; said membrane comprising an active layer comprising immobilized aquaporin water channels and a support layer, and said membrane having a feed side and a non-feed side; and an aqueous source solution in fluid communication with the feed side of the membrane.

The present invention relates to methods and means for ammonia and / or ammonium transport in a variety of organisms, including mammals, yeast and plants. In particular, the present invention is related to the use of isolated polypeptide molecules, which are particular members of the aquaporin superfamily, and isolated nucleic acid molecule that encode such polypeptides in the transport of NH4+ / NH3 across a membrane.