37 results about "Elastin peptides" patented technology

Relating to the field of biotechnology, disclosed are a recombinant human elastin peptide with anti-aging effects and a use thereof. The recombinant human elastin peptide comprises an amino acid sequence shown in SEQ ID NO:1 or a fragment of the amino acid sequence shown in SEQ ID NO:1, the fragment comprises 30% or more of SEQ ID NO:1, or a mutant of the amino acid sequence shown in SEQ ID NO:1 or the fragment of the amino acid sequence shown in SEQ ID NO:1. A disclosed method for producing elastin peptides by using a yeast expression system enables the targeted synthesis of highly effective protein fragments with 100% homology to human proteins, while achieving low costs and facilitating large-scale production, avoiding animal-derived sources and viral risks. This makes the recombinant human elastin peptide safe for use as an anti-aging and anti-photoaging active ingredient in cosmetics, while also supporting a use thereof in the field of biomedical materials.

The invention discloses a preparation method of elastin peptide, and relates to the technical field of natural elastin extraction.The preparation method comprises the following steps that S1, animal blood vessels are cleaned, subjected to impurity removal, cut into blocks and minced; s2, the diced and minced animal blood vessels are soaked in alkali liquor for degreasing, the animal blood vessels are fished out and cleaned after degreasing, water is added after cleaning, pulping is conducted, slurry is obtained, and the pH of the slurry is adjusted to 7.5-8.5; s3, adding elastinase and trypsin into the pulp, and performing enzymolysis to obtain enzymatic hydrolysate; s4, sequentially performing high-temperature enzyme deactivation, vacuum concentration, purification, refining and spray drying on the enzymatic hydrolysate to obtain powdery elastin peptide. The trypsin and elastinase adopted in the invention belong to basic proteins, can decompose proteins under alkaline conditions, and have the characteristics of high catalysis efficiency, action specificity, activity adjustability, diversity and the like.

The invention discloses a special diet and a preparation process thereof, and the special diet comprises the following raw materials: 500-600 parts of a red date concentrated solution, 350-400 parts of purified water, 40-60 parts of collagen peptide, 4-6 parts of lycium ruthenicum primary pulp, 0.5-1.5 parts of donkey-hide gelatin oligopeptide powder, 0.5-1.5 parts of longan powder, 3-5 parts of gamma-aminobutyric acid essence, 0.5-1.5 parts of elastin peptide, 0.5-1.5 parts of cubilose peptide, and 0.5-1.5 parts of collagen tripeptide. And 0.5 to 1.5 parts of epigallocatechin gallate. According to the special diet disclosed by the invention, the matching of the red date concentrated solution and the lycium ruthenicum primary pulp not only provides abundant natural antioxidant substances, but also enhances the blood replenishing and beautifying functions of the product, and the combination of the collagen peptide, the elastin peptide and the collagen tripeptide provides multi-level nourishing protection and the like for skin and joint health. The whole preparation process is accurate and controllable in technological parameters, high in production efficiency and suitable for large-scale production, and the obtained product has the remarkable characteristics of being fine and smooth in taste, definite in effect and good in stability and can meet the diversified requirements of different people for functional dietary supplements.

The invention relates to the technical field of functional drinks, in particular to a polypeptide synergistically strengthened cubilose collagen composite drink with a breast enlarging function and a preparation method of the polypeptide synergistically strengthened cubilose collagen composite drink. The drink contains fish collagen tripeptide, elastin peptide, cubilose peptide, pueraria mirifica isoflavone, red clover isoflavone and saffron crocus extract. A lipidosome delivery system is adopted to entrap polypeptide to form a nano-scale compound, nano-emulsion entraps phytoestrogen, and berry polyphenol and lipidosome-polypeptide form a stable ternary compound. Through the multi-way synergistic effect that phytoestrogen activates a mammary gland estrogen receptor, polypeptide promotes connective tissue collagen synthesis and cubilose peptide activates a growth factor signal, mammary gland development is remarkably promoted, the breast enlarging effect is achieved, the bioavailability reaches 85% or above, and the retention rate of active ingredients stored at normal temperature for 24 months is larger than 90%.

The present invention relates to the field of biotechnology and discloses a recombinant human elastin peptide with anti-aging efficacy and its application in a composition thereof. The recombinant human elastin peptide comprises the elastin peptide amino acid sequence SEQ ID: 1 or a fragment thereof, wherein the fragment comprises 30% of SEQ ID: 1, or a fragment comprises more than 30% of SEQ ID: 1, or a mutant of the above sequence. The recombinant human elastin peptide can be directed to synthesize a protein of a highly effective fragment, with a 100% human homologous sequence, while being low-cost, easy to mass-produce, avoiding animal sources, and avoiding viral risks. This allows the recombinant human elastin peptide to be safely used in cosmetics as an anti-aging and anti-photoaging ingredient, while also providing support for its application in the field of biomedical materials.

The application discloses a functional composition for improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component; the first active component comprises elastin peptide, haematococcus pluvialis and ergothioneine; and the second active component comprises one or more of houttuynia cordata and non-denatured type II collagen. The application realizes the protection effect on joint, cartilage and bone health by scientific matching of the components and synergistically improving the glycosaminoglycan level in chondrocytes and the osteocalcin level in osteoblasts. Meanwhile, the components in the functional composition for improving bone and joint health are natural and have good biocompatibility, and have no obvious side effects, so that the application provides a kind of drug or a substitute for the treatment and prevention of bone diseases.

The invention discloses a multifunctional special dietary food skin beautifying and shaping formula and application, and belongs to the technical field of beauty treatment. The multifunctional special dietary food at least comprises collagen peptide, collagen tripeptide, skipjack elastin peptide, carob bean extract, phosphatidylserine, casein hydrolytic peptide, nicotinamide, rice bran oil powder, haematococcus pluvialis, cranberry powder, oriental cherry flower powder, pig blood peptide powder, red date peptide powder and cattle spleen peptide. The health-care product can comprehensively supplement nutrition recovered after exercise, has the effects of beautifying, shaping and removing wrinkles, can effectively relieve pressure and regulate emotion, and also has the effects of improving female physiological health, strengthening the spleen and stomach and enhancing immunity.

The present invention provides a process for extracting collagen tripeptide (CTP) from collagenase hydrolysate, comprising the following steps: performing two enzymatic hydrolysis on the pretreated collagen raw material, filtering, and adopting specific magnetic microsphere separation and molecular imprinting polymer microsphere medium solid phase extraction purification. The present invention can obtain a high-content (up to 80%) collagen tripeptide composition product (Gly-X-Y, including Gly-Pro-Hyp). The present invention has the advantages of high separation efficiency, low cost, and suitability for industrial-scale production. Studies have shown that the compound use of collagen tripeptide and elastin peptide in a ratio of 16:1 to 24:1 can significantly increase the content of elastin and collagen in the skin, and significantly increase the content of hyaluronic acid and hydroxyproline, while reducing the content of MMP3 and inhibiting skin inflammatory factors.

The invention discloses a functional composition capable of improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component, the first active component is prepared from elastin peptide, haematococcus pluvialis and ergothioneine; the second active component is prepared from one or more of herba houttuyniae and non-denatured type II collagen. Through scientific compatibility of all the components, the levels of glycosaminoglycan in chondrocytes and osteocalcin in osteoblasts are synergistically improved, and the protection effect on joint, cartilage and skeleton health is achieved. Meanwhile, a plurality of components in the functional composition capable of improving the health of the bones and the joints are natural in source, good in biocompatibility and free of obvious side effects, and a medicine or an alternative scheme thereof is provided for treatment and prevention of the bone diseases.

The invention provides gel based on collagen liposome. The gel comprises the following components in parts by mass: 2 parts of shikonin liposome; 2 parts of collagen peptide liposome; 1 part of elastin peptide liposome; and 1 part of carbomer. According to the gel based on the collagen liposome provided by the invention, the alkannin liposome compound gel has an obvious cleaning capability, namely, the alkannin liposome compound gel has a very strong anti-oxidation capability. The alkannin liposome compound gel has an obvious cleaning effect on H2O2 (hydrogen peroxide). The alkannin liposome compound gel has an excellent itching relieving effect. The alkannin liposome compound gel has the effect of resisting eczema. The alkannin liposome compound gel also has an inhibition effect on staphylococcus aureus and escherichia coli. The invention further provides a preparation method of the gel based on the collagen lipidosome and application of the gel based on the collagen lipidosome in the aspect of drugs for treating skin diseases.

To suppress an unpleasant odor when an iron compound and collagen peptide or elastin peptide are formulated in an oral liquid composition.SOLUTION: The oral liquid composition comprises (A) an iron compound, (B) at least one peptide selected from the group consisting of collagen peptide and / or elastin peptide, and (C) at least one plant extract selected from the group consisting of lemon balm extract, Houttuynia cordata extract, artichoke extract and rosemary extract.SELECTED DRAWING: None

The invention discloses a high-content astaxanthin beverage and a preparation method thereof. The product contains 0.6-2% of astaxanthin microcapsule powder or astaxanthin aqueous solution, and also contains fish collagen peptide, skipjack elastin peptide, sodium hyaluronate, nicotinamide, honey, vitamin B6, pectin, lactic acid, citric acid, fermented kiwi fruit juice, fermented pineapple juice, fermented lemon juice and the like. By optimizing the formula and the process, the problems of low astaxanthin content, poor stability and single nutrition function in the existing astaxanthin beverage are successfully solved, and the astaxanthin beverage with higher quality and higher efficiency is provided for consumers.

The invention discloses a composition for repairing pelvic floor muscle and regenerating elastic fiber and a preparation method thereof, and relates to the technical field of pelvic floor muscle repairing, the composition is prepared from the following raw materials by weight: 10-50 parts of collagen peptide, 5-30 parts of elastin peptide, 5-40 parts of metagen, and 20-100 parts of a traditional Chinese medicine extract group, according to the composition for repairing pelvic floor muscles and regenerating elastic fibers and the preparation method of the composition, fish collagen peptide, deep sea gill fish elastin peptide, metabiotics and a traditional Chinese medicine extract group are used as raw materials, so that a composition system with multiple target points and a synergistic effect is constructed; according to the present invention, the pelvic floor muscle structure and the elastic fiber are systematically repaired and regenerated, the problems of urine leakage, pelvic floor muscle relaxation and the like are effectively improved, the lactic acid bacteria powder VGA1 and the casei paracasei are adopted to carry out liquid-state and solid-state combined fermentation, and the cell-penetrating peptide, the targeting peptide and other active substances are effectively enriched, such that the pelvic floor muscle structure and the elastic fiber are effectively repaired and regenerated; therefore, the bioavailability and the targeting effect efficiency of the functional components in the composition are improved.

This invention discloses an oral liquid containing astaxanthin and its preparation method, which is prepared from raw materials comprising the following weight percentages: 0.5%~2% astaxanthin burst beads, 8%~12% collagen peptides, 0.1%~0.3% sodium hyaluronate, 0.01%~2% elastin peptides, 0.01%~0.05% spermidine, 0.005%~0.02% ergothioneine, and 0.001%~0.01% pyrroloquinoline quinone. The astaxanthin burst beads are prepared using astaxanthin oil as the core material and sodium alginate and chitosan as the wall material. This invention, by using astaxanthin burst beads with higher stability and bioavailability in the oral liquid, combined with other ingredients, can improve the user's skin moisture content and elasticity index, reduce wrinkle depth, reduce skin yellowing, and better exert the effects of the oral liquid.

The present invention belongs to the technical field of active substance extraction, and specifically relates to a low-fat, low-cost, high-purity and high-quality elastin peptide and a preparation method thereof. The present invention uses a mixture of alkali solution and sodium carbonate solution, the overall concentration is low, it is more environmentally friendly, and the degreasing effect is better using microwave heating technology. The present invention uses microsphere immobilized protease, and the enzyme preparation can be fully recovered and reused multiple times, which greatly reduces production costs. The elastase hydrolysate is separated, purified and refined using ion exchange resin series chromatography technology to efficiently remove other impurities, and the prepared elastin peptide has a higher purity. The present invention uses high-voltage pulsed electric field technology (PEF) for sterilization, which has a better sterilization effect, avoids the influence of traditional heat sterilization methods on the color and taste of the product, and better maintains the quality of the elastin peptide.

The invention provides a preparation method and application of a camptothecin-elastin-like peptide conjugate capable of being self-assembled, and belongs to the field of nano-drugs. The camptothecin self-assembled prodrug is synthesized through a drug-connexon conjugate, and the obtained camptothecin self-assembled prodrug can be self-assembled in an aqueous solution through pi-pi interaction among camptothecin molecules to form a one-dimensional nanotube structure. The camptothecin self-assembled prodrug adopts the design concept of self-assembled prodrug, camptothecin is used as a structural unit, the camptothecin self-assembled prodrug with hydrophilic and hydrophobic balance is obtained by simply connecting hydrophilic polypeptides with different sequences, the prodrug has high drug loading capacity and improved solubility, and a clearly defined one-dimensional nano structure can be formed by simply dissolving the prodrug in water. The method has the advantages that the method is simple and easy to operate, the repeatability is excellent, the tumor inhibition effect is obviously superior to that of clinical medicine irritecan in in-vivo anti-tumor treatment, nearly 90% of tumor growth inhibition can be achieved, the biological safety is good, and the method has important significance in nano-medicine production preparation and clinical conversion.

The invention discloses a recombinant elastin capable of improving skin elasticity and a preparation method and application thereof, and relates to the technical field of genetic engineering. According to the recombinant elastin capable of improving skin elasticity, the amino acid sequence of the recombinant elastin is shown as SEQ ID: 1, and the nucleotide sequence for coding the recombinant elastin is shown as SEQ ID: 2. The recombinant elastin has better antioxidant activity than commercial elastin and natural elastin, can be applied to biological skin care, and has an obvious anti-wrinkle effect.

The invention relates to medicinal oral liquid of glycosaminoglycan and a preparation method, and belongs to the technical field of biological medicines.The oral liquid adopts glycosaminoglycan-rich collagen peptide, cod collagen oligopeptide powder, skipjack elastin peptide-Y, fish maw oligopeptide powder, fish collagen tripeptide, apple concentrated juice, white birch juice and the like as effective active ingredients, the apple concentrated juice is obtained by sequentially carrying out step-by-step enzymolysis on green apples through bromelain, ficin and trypsin; the white birch juice is obtained by carrying out anaerobic fermentation on white birch juice through lactobacillus brevis and lactobacillus cellosus. All the components are matched for use, so that the food has very good effects of reducing blood fat, increasing bone mineral density, improving immunity, resisting fatigue and the like, and the action effect is rapid and effective.

The invention belongs to the technical field of candy preparation, and particularly relates to a collagen peptide, donkey-hide gelatin and camellia oil gel candy and a preparation method thereof.The collagen peptide, donkey-hide gelatin and camellia oil gel candy is of a double-material structure comprising stuffing and a candy body, the camellia oil comprises the following components: 46%-50% of camellia oil, 4%-9% of fish collagen peptide powder, 3%-8% of glutathione yeast powder, 3%-8% of skipjack elastin peptide, 3%-8% of mono / diglycerol fatty acid ester, 3%-8% of donkey-hide gelatin powder, 1%-3% of ginseng powder, 2%-7% of pomegranate fruit powder and 2%-7% of blueberry fruit powder. The sugar body comprises the following components in percentage by dry weight: 28%-38% of gelatin, 42%-52% of sorbitol, 14%-19% of purified water, 6%-11% of glycerol and 0.5%-1% of caramel color; the weight ratio of the sugar body to the stuffing is 7: 3, and through the synergistic effect of multiple components, the three core effects of skin care, nutrition tonifying and intestine and stomach conditioning are achieved; meanwhile, through process optimization, the hardness is stable, the taste is elastic, the heavy metal and microbiological indexes meet the safety standard, and the method is suitable for large-scale production and application in the field of healthy food.

The invention discloses compact anti-wrinkle elastin peptide freeze-dried powder and a preparation method thereof, and belongs to the technical field of bioactive peptides. The freeze-dried powder is prepared by taking core tubes and / or throat tubes of bred animals as raw materials through cleaning and impurity removal, crushing and homogenizing, degreasing and impurity removal, composite enzymolysis, enzymolysis inactivation, centrifugal separation, gradient filtration, concentration, sterilization and vacuum freeze drying, and has remarkable elastinase inhibitory activity and tightening and anti-wrinkle effects. The livestock and poultry slaughtering byproducts which are wide in source and low in cost are selected as raw materials, high-value utilization of the livestock and poultry slaughtering byproducts is achieved, the optimized preparation technology solves the problems that in the prior art, enzymolysis efficiency is low, product purity is low, activity is insufficient, and stability is poor, the technology is simple, large-scale production is easy, and the method can be widely applied to the fields of cosmetics, food and health care products.

The present invention belongs to the field of flavored beverages, and specifically relates to a weakly acidic high-content collagen beverage and a preparation method thereof. The weakly acidic collagen beverage is composed of the following raw materials: collagen peptides, collagen tripeptides, elastin peptides, sweeteners, plant powders and solvents. The present invention applies online bottle blowing (small package PET bottle 60-70mL), aseptic cold filling and UHT sterilization technology to collagen beverages, which solves the problem that hot filling or post-high temperature sterilization processes must be adopted in the production process of existing collagen beverages, reduces the duration of collagen beverages in high temperatures, and reduces the protein denaturation of proteins in collagen peptides under high temperature conditions. The present invention expands the pH value of collagen beverages from ≤4.6 to ≤5.6, broadening the scope of use of acid-sensitive materials. The collagen beverage has excellent taste, high collagen content, weakly acidic pH, no bad flavor, stable quality, and no preservatives are added.

The invention discloses a fish peptide compound with anti-oxidation, anti-wrinkle and whitening functions as well as a preparation method and application thereof, belongs to the technical field of functional peptide application, and provides the fish peptide compound with the anti-oxidation, anti-wrinkle and whitening functions. Comprising 40 to 50 parts of fish collagen peptide, 36 to 40 parts of elastin peptide, 18 to 21 parts of collagen tripeptide, 14 to 17 parts of tuna active peptide, 5 to 6 parts of avocado extract, 2 to 4 parts of fish peptide and 2.6 to 3.0 parts of rye seed extract. According to the fish peptide compound with the antioxidant, anti-wrinkle and whitening functions as well as the preparation method and the application, the fish-derived functional peptide and the plant extract are reasonably compounded, all the components play a synergistic role, and the prepared fish peptide compound has the antioxidant, anti-wrinkle and whitening effects at the same time.

The application discloses a functional composition, a conditioner and a use for treating or preventing bone and joint diseases. The composition of the functional composition comprises a first active component and a second active component, wherein the first active component comprises elastin peptide 1-500 mu g / mL, haematococcus pluvialis 40-20 mu g / mL, phyllanthus emblica 8-4 mu g / mL and mussel 1-500 mu g / mL in terms of concentration in the functional composition; and the second active component comprises broccoli water extract and / or boric acid substances. The application realizes the synergistic improvement of glycosaminoglycan and osteocalcin levels by scientific compounding, plays a protective role on joint, cartilage and bone health, and has natural, safe, good biocompatibility and no obvious side effects, and provides a kind of drug or a replacement scheme for bone disease treatment and prevention.

The invention discloses a composition for promoting skin repair, a skin conditioner and cosmetics. The composition for promoting skin repair is prepared from 0.1 mg / mL to 3 mg / mL of elastin peptide, 1 mg / mL to 30 mg / mL of collagen tripeptide and 0.1 mg / mL to 0.5 mg / mL of aronia melanocarpa, wherein the aronia melanocarpa is prepared from an aronia melanocarpa raw material and / or an aronia melanocarpa extract. The elastin peptide, the collagen tripeptide and the aronia melanocarpa are scientifically matched, so that the synergistic functional activity is realized, the cell migration rate is remarkably improved, the gene expression level of inflammatory cytokines is remarkably reduced, wound healing and inflammation relieving are accelerated, and skin repair is realized. Meanwhile, the components in the composition are all natural sources, and the composition has good biocompatibility and safety and is suitable for being added into various skin repairing functional products.

The invention provides an enzymolysis method of horse ligament elastin peptide, and relates to the technical field of enzymolysis of protein peptide.The enzymolysis method comprises the steps that the optimum temperature needed by enzymolysis is adjusted; adjusting the pH value of the initial mixed solution to the optimum pH range of the enzyme; optimizing the enzyme-substrate ratio by using the rough set and RBF neural network fusion modeling; adding the selected enzyme to carry out enzymolysis operation; the enzymatic hydrolysate is rapidly heated, the enzymatic hydrolysis reaction is terminated, and a protein peptide mixture is obtained; and separating and purifying the protein peptide mixture to obtain the high-purity elastin peptide. According to the method, the enzyme-substrate ratio is optimized by utilizing rough set and RBF neural network fusion modeling, and the optimal enzyme-substrate ratio is determined in combination with multiple means such as cross validation and gradient descent, so that the enzymolysis process is more scientific and accurate, and the yield of elastin peptide is remarkably increased.

Liquid oral composition as a food, food supplement or dietary food for improving skin elasticity, skin structure and supporting joint function, comprising hydrolyzed collagen peptides obtained from cod (Gadus spp. especially Gadus morhua) and hydrolyzed elastin peptides, characterized in that it is available as a ready-to-drink or instant powder-soluble oral solution and achieves a synergistically enhanced effect compared to conventional collagen-elastin combination preparations due to the combined, complementary amino acid profile of the active ingredients.

The invention relates to the technical field of food processing, and discloses a collagen solid beverage formula and a production technology, and the solid beverage comprises the following components: cod collagen peptide; inulin; blood orange powder; acerola cherry powder; gamma-aminobutyric acid; skipjack elastin peptide; blueberry powder; sodium hyaluronate; the production process comprises the following steps: sieving the raw materials for later use; the preparation method comprises the following steps: firstly, mixing part of cod collagen peptide with total amount of gamma-aminobutyric acid, skipjack elastin peptide, blueberry powder, sodium hyaluronate and food essence to obtain a first-grade mixture, then uniformly mixing the first-grade mixture with the remaining cod collagen peptide, total amount of inulin, blood orange powder and acerola cherry powder, and finally, performing metal detection and nitrogen-filled packaging to obtain a finished product. Through a mixing process, the moisture absorption agglomeration risk of the high-hygroscopicity raw material is reduced, the problem of uneven mixing of trace active ingredients is solved, and the obtained product is quick to dissolve, good in dispersity and uniform in nutritional ingredient.

The application discloses an elastin peptide and a preparation process thereof, and relates to the technical field of deep processing. The elastin peptide and the preparation process thereof are designed in the application. Through specific pretreatment degreasing process and two-stage enzymolysis process, the influence of fat substances in the elastin peptide on the use effect of the elastin peptide is greatly reduced, the biological activity of the elastin peptide is retained, the contents of desmosine and isodesmosine are improved, the elastin peptide is applied to skin anti-wrinkle, the skin elasticity can be obviously enhanced, and good anti-wrinkle effect can be achieved, and the elastin peptide has good application value.

The invention discloses a functional composition for treating or preventing bone and joint diseases, a conditioner and application. The functional composition comprises a first active component and a second active component, and according to the concentration of the functional composition, the first active component comprises 1 [mu] g / mL-500 [mu] g / mL of elastin peptide, 40 ng / mL-20 [mu] g / mL of haematococcus pluvialis, 8 ng / mL-4 [mu] g / mL of emblic leafflower fruit and 1 [mu] g / mL-500 [mu] g / mL of mussel; the second active component comprises a broccoli aqueous extract and / or boric acid substances. Through scientific compatibility, all the components synergistically improve the levels of glycosamine poly and osteocalcin, the health of joints, cartilages and bones is protected, the components are natural in source, safe, good in biocompatibility and free of obvious side effects, and a medicine or an alternative scheme thereof is provided for treatment and prevention of bone diseases.

The invention relates to the field of food, in particular to an elastin peptide-containing composition with anti-wrinkle and firming effects and a preparation method thereof. The composition with the anti-wrinkle and firming effects is prepared from the following components in parts by weight: 15 to 25 parts of elastic-collagen binding peptide powder, 8 to 12 parts of red beet juice powder, 4 to 6 parts of elderberry juice powder, 1 to 4 parts of fish maw oligopeptide powder, 1 to 4 parts of pleurotus citrinopileatus extract and 15 to 20 parts of resistant dextrin. The components in the formula are compounded according to a certain content, a remarkable synergistic effect can be generated, the content of elastin and collagen in skin is effectively increased, the skin elasticity is improved, wrinkles are effectively reduced, and skin aging is delayed.