The invention provides regeneration and repair cold compress gel as well as a use method and application thereof. The regeneration and repair cold compress gel comprises shea butter, lactic acid, jojoba oil, a witch hazel extract, urea, glycerin, panthenol, nicotinamide, tetrapeptide, phenoxyethanol, triethanolamine salicylate, D-camphor, eucalyptol, turpentine, collagen, carbomer, allantoin, sodium hyaluronate, octenidine, calcium gluconate and water. According to the regeneration and repair cold compress gel as well as the use method and application thereof, the regeneration and repair cold compress gel can increase the skin barrier density, promote the repair of cracked skin, promote skin regeneration, keep the skin moist, improve the skin smoothness, improve the skin softness, reduce pathogenic bacteria and virus residues, relieve skin inflammation and eczema symptoms, relieve skin allergy and pruritus, and relieve the pain caused by rheumatism, arthritis and muscle injury.