The invention provides a non-treatment-purpose
confocal detection method for chemical
efficacy based on a zebra fish model and an application of the non-treatment-purpose
confocal detection method. According to the application, the KRT4 transgenic red fluorescent line
zebrafish is utilized, and UVA damage and
image acquisition are carried out through a
confocal microscope. The
sunscreen effect is detected, the bottom of a culture dish is coated with a tested chemical to form a film, and the
sunscreen effect is quantitatively evaluated by comparing the
skin fluorescence intensity weakening degree of a sample group and a model group after UVA
irradiation; and detecting the repair effect, incubating the damaged
zebrafish by using a tested
chemical solution, and quantitatively evaluating the repair effect by comparing the epidermis
fluorescence intensity
recovery degrees of the incubated sample group and the model group. According to the method, the
living body biological model is combined with the confocal
microscope UVA damage and
imaging technology, direct, quantitative, visual and high-
throughput evaluation on the
efficacy of the chemicals is achieved, and the defects that a traditional in-vitro method is not close to living bodies, and
human body tests are high in cost and long in period are overcome.