The present invention relates to the field of
plant tissue culture technology, and in particular to a sterile
cyperus chinensis
seedling and a cultivation method thereof. The method of the present invention selects
cyperus chinensis
bud points as explants. Compared with the whole tuber, the volume is smaller, the
pollution source is reduced, and there is no need to additionally disinfect and culture the tuber. Only the stripped
bud points need to be disinfected for 0.5 to 5.0 minutes. Specifically, a
low mass concentration of 2.5%-7.5%
sodium hypochlorite solution can be used for disinfection, which reduces the concentration and disinfection time of the
disinfectant and reduces the damage of the disinfection process to the
bud points. The method is simple to operate, time-saving and efficient, and effectively solves the problems of high
contamination rate and low
germination rate in the preparation process of
cyperus chinensis sterile seedlings, thereby greatly improving the efficiency of cyperus chinensis sterile
seedling preparation. Furthermore, the method of the present invention optimizes and selects culture media of different cultivation processes, has a short bud
induction time, a high proliferation coefficient, and robust growth, and does not require the addition of any
growth regulator during root induction, resulting in low cost.