The invention discloses a Chinese herbal medicine for killing insects in aquatic culture. The Chinese herbal medicine is prepared from the following medicinal materials according to a proportional ratio by mass: 10 to 15 parts of Chinaberry barks and 3 to 5 parts of Chinaberry fruits, and is prepared by the following steps of putting the Chinaberry barks and the Chinaberry fruits into a medicine boiler according to the proportional ratio, adding 500 to 800 parts of drinking water according to a mass ratio, heating, boiling for 30 to 45h, filtering, removing medicine residues, and collecting amedicine liquid; adding 200 to 300 parts of drinking water into the filter residues, heating, boiling for 20 to 30min, filtering, removing filtering residues, and mixing the obtained medicine liquid and the medicine liquid after primary boiling. A use method of the Chinese herbal medicine is characterized in that 12L of medicine liquid is sprayed in every 627m<3> of area, and the medicine liquid is sprayed once each day, and is continuously used for 2 to 3 days. The Chinese herbal medicine preparation has the advantages that the Chinese herbal medicine preparation is used for preventing and controlling the insects in aquatic culture, such as fish parasites, and the parasite bodies can be paralysed by the Chinese berry base and tannin, be strongly and lastingly shrunk, and die; the nerves of the parasites can be poisoned by the Chinaberry barks and fruits; the functions of inhibiting, repelling and killing the insects are realized; the effect is quickly released, and is quickly taken, so as to quickly solve the problem of infection of fish diseases.