The invention provides a mussel mucoprotein gel for wound repair, and a preparation method and an application thereof. The mussel mucoprotein gel for wound repair comprises the following raw materials in percentage by weight: 0.15-0.2% of mussel mucoprotein, 0.1-5% of protein protector, 0.1-5% of tackifier, 0.1-5% of stabilizer, 0.1-5% of preservative, 1-10% of wetting agent, 0.1-5% of pH (potential of hydrogen), and 64.8-98.35% of medical dissolving water. With Carbomer as a substrate, the mussel mucoprotein gel has good extending property, is easy to coat and adhere on a skin, has no irritation to skin and mucosa and can absorb tissue penetrating fluid, so secretions can be exhausted conveniently; the gel is not greasy, the medicines are released fast, and the coupling effect with skin tissues is excellent.