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209 results about "Drop method" patented technology

Method for freezing and reviving umbilical cord tissues and for separating and increasing stem cells

The invention relates to a method for freezing and reviving umbilical cord tissues and for separating and increasing stem cells after reviving the umbilical cord, and the step comprises the following steps of preparing umbilical cord tissue freezing liquid; sterilizing and washing the umbilical cord tissues; cutting the tissues into blocks; placing the tissue blocks and the freezing liquid into afreezing tube, cold storing the tissue blocks for 0.5 hour under the temperature condition of 4 DEG C and then storing the tissue blocks for one day under the temperature condition of minus 80 DEG C,and then freezing the tissue blocks in liquefied nitrogen; and taking the umbilical cord tissues out of the liquefied nitrogen to use, thawing the umbilical cord tissues in constant-temperature waterbath, utilizing mesenchymal stem cell culture base to washing the umbilical cord tissues through a drop method, and separating and increasing the mesenchymal stem cells of the revived umbilical cord tissues through a tissue attachment method. The method can effectively protect the frozen umbilical cord tissue, so that the umbilical cord tissues can be revived to use, and the method is particularly suitable for separating and increasing the mesenchymal stem cells after the umbilical cord tissue is revived.
Owner:BOYALIFE

Method for freezing and thawing placental whole cells and separating and expanding stem cells

The invention relates to a method for freezing and thawing placental whole cells and separating and expanding stem cells. The method comprises the steps as follows: disinfecting and washing a placenta tissue; cutting off placental lobules from the tissue, and carrying out digestive treatment for 20 min; preparing a placenta tissue freezing solution for standby application; adding whole cells obtained by digestive treatment and the freezing solution into a freezing tube, refrigerating for 0.5 h at the temperature of 4 DEG C, freezing for 1 day at the temperature of subzero 80 DEG C, and freezing in liquid nitrogen for standby application; taking out the placental whole cells from the liquid nitrogen when needed, thawing in a thermostatic water bath, carrying out drop-method washing by a culture medium for mesenchymal stem cells, removing red cells by a red cell lysis solution, and expanding the mesenchymal stem cells by the thawed placental whole cells through cell culture and cell passage. According to the method, the frozen placenta tissue can be effectively protected and is convenient to thaw and use; and the method is in particular suitable for separating and expanding the mesenchymal stem cells after thawing the frozen placental tissue.
Owner:BOYALIFE

System and method for calculating feasible operation range of BHKW under condition of ordering power by heat

The invention discloses a system for calculating a feasible operation range of a BHKW under the condition of ordering power by heat. The system comprises a data collecting and analyzing module, a working condition changing calculating module, a working condition graph module and a peak adjusting load reading module which are sequentially connected, wherein the data collecting and analyzing module reads operation data from a power plant DCS and obtains a representation actual operation benchmark working condition point through the clustering methodology method, and the working condition changing calculating module carries out working condition changing calculation based on the equivalent enthalpy drop method, extracts a working condition changing characteristic according to the optimum square approach method of a continuous function and obtains an actual operating working condition curve of the BHKW. The working condition graph module is obtained according to a working condition changing characteristic curve. The peak adjusting load reading module intelligently calculates the feasible operation range of a peak adjusting load from a working condition graph based on a database. The invention further provides a method for calculating the feasible operation range through the system. The system and method can be used for carrying out operation calculation of a large combined heat and power generation single drawing unit and a large combined heat and power generation double-drawing unit, and a heat supply unit is arranged to adjust the peak, so that thermal power plant and power grid dispatching is greatly facilitated.
Owner:ELECTRIC POWER RES INST OF GUANGDONG POWER GRID

Low-altitude positioning load air-drop method based on multi-rotor unmanned aerial vehicle

The invention discloses a low-altitude positioning load air-drop method based on a multi-rotor unmanned aerial vehicle. The multi-rotor unmanned aerial vehicle comprises an aerial flight platform, onboard task equipment and a ground remote control device. The onboard task equipment is located below the aerial flight platform. The onboard task equipment comprises an air-drop engine, a positioning camera and a directional voice broadcast device. The method solves the problem that air-drop of badly-needed emergency supplies is difficult in emergency rescue. The multi-rotor unmanned aerial vehicle has the advantages of being low in cost, easy and convenient to operate and easy to maintain and facilitating transport; with the low-altitude hovering function of the multi-rotor unmanned aerial vehicle and the assistance of the onboard assistant task equipment such as the voice broadcast device and the positioning camera, material air-drop accuracy and utilization efficiency are improved. The method has outstanding application value for a small number of loads which is light and is badly needed by trapped persons, and has great popularization potential in the field of air-drop of emergency rescue supplies.
Owner:HEFEI INST FOR PUBLIC SAFETY RES TSINGHUA UNIV

Method using 3D printing technology to prepare dropping pills

The invention relates to a method using the 3D printing technology to prepare dropping pills. The method using the 3D printing technology to prepare the dropping pills comprises the steps of (1) using polyethylene glycol and the like as base materials, carrying out heating melting, (2) adding prescription drugs at the specified temperature, carrying out mixing, even stir and freeze drying to obtain solid powder, carrying out sieving on the solid powder, and (3) placing the solid powder into a powder box of a 3D printer, and preparing the dropping pills through electronic drafting, remote data transmission and laser scanning printing. According to the method using the 3D printing technology to prepare the dropping pills, due to the facts that through a series of technologies such as the electronic design technology, the remote data transmission technology, the laser scanning technology and the raw material and auxiliary material melting technology, the specific drug powder materials are melted, and electronic model graphs are changed into the real dropping pills according to designed indications, the advantages of the dropping method of preparing the dropping pills are kept, and the defects that due to the influence of the factors such as physical properties of liquid medicine, droppers and cooling liquid, pill weights are different, the roundness of pills is difficult to control, the percent of pass is low, and specially-shaped dropping pills cannot be easily prepared when the dropping method is used for preparation are overcome.
Owner:魏雪纹

Methods for freezing and thawing whole cell of umbilical cord and separating and augmenting stem cell

The invention relates to methods for separating, freezing and thawing a whole cell of an umbilical cord and separating and augmenting a thawed stem cell. The method for separating and freezing the whole cell of the umbilical cord comprises the following steps of: sterilizing and cleaning an umbilical cord tissue; shearing the tissue into a block shape and carrying out digestion treatment for 1.5 hours; preparing a umbilical cord tissue frozen solution for later use; and adding the whole cell and frozen solution which are obtained by sterilizing treatment into a freezing tube, refrigerating the freezing tube for 0.5 hour at low temperature of 4DEG C, freezing the freezing tube for one day under the temperature condition of -80DEG C and then freezing the freezing tube in liquid nitrogen for later use. The method for thawing the whole cell of the umbilical cord comprises the following steps of: when the whole cell of the umbilical cord is required, extracting the whole cell of the umbilical cord from the liquid nitrogen, thawing the whole cell of the umbilical cord in a constant-temperature water bath; cleaning the whole cell of the umbilical cord by using a mesenchymal stem cell culture medium and a drop method; and augmenting a mesenchymal stem cell by using the thawed whole cell of the umbilical cord through cell culture and cell passage. According to the methods disclosed by the invention, the frozen umbilical cord tissue can be effectively protected and is convenient for thawing; and the method is especially suitable for separating and augmenting the mesenchymal stem cell after the frozen umbilical cord tissue is thawed.
Owner:BOYALIFE

High-temperature and high-pressure interfacial tension measurement device and method

InactiveCN104155217ASolve the need for high pressure resistanceSolve high temperature resistanceSurface tension analysisSapphire windowMeasurement device
The invention provides a high-temperature and high-pressure interfacial tension measurement device and method. The measurement device is provided with a buffer bottle and a gas injection pump between a steel cylinder and a visual kettle; a sample injection pump is connected with a pump flow control box and a pump temperature measurement probe; the gas injection pump is arranged in a cold trap which is connected with a low-temperature thermostatic bath; the gas injection pump is provided with an outer cavity and an inner cavity; a window of the visual kettle is a sapphire window; a temperature measurement probe and a pressure sensor in the kettle are respectively arranged on the two sides of a suspension dropper; a gas-liquid inlet and outlet pipe is vertically opposite to the suspension dropper at the bottom of the visual kettle. After the device and the method are adopted, the problems that pressurization is difficult when a pendant-drop method is used for interfacial tension measurement under the conditions of high temperature and high pressure, liquid drops easily fall off under mixed-phase condition, and the window needs high pressure resistance, high temperature resistance and high light transmittance can be solved; an experimental facility is safe and explosion-proof; the device and the method for interfacial tension measurement within the high experimental temperature range (over 100 DEG C) and under the high pressure (over 10 MPa) can be provided.
Owner:TIANJIN UNIV

Cold-strip steel edge drop method through working roll asymmetric shifting control

The invention provides a cold-strip steel edge drop method through working roll asymmetric shifting control, which comprises the steps that the width of entrance strip steel serves as feedforward presetting control; practical convexity degrees of the two sides of an incoming material are measured by an entrance multipoint convexity gauge, and tapered section insertion quantities of tapered working rolls on a driving side and an operation side are calculated by the feedforward control and serve as the feedforward presetting control; an incoming material running deviation measured by the entrance multipoint convexity gauge serves as the feedforward presetting control; practical edge drops of the two sides of finished strip steel are measured by an exit multipoint edge drop gauge, and the tapered section insertion quantities of the tapered working rolls on the driving side and the operation side are calculated by a feedback closed-loop control model; and according to position changes of the single-taper working rolls on the driving side and the operation side, bending compensation control on the working rolls is performed. Through independent shifting of an upper working roll and lower working roll, and independent roll bending of the working rolls on the operation side and the driving side, independent control on the thicknesses of the two side edges of the strip steel is realized, the incoming material with different convexity degrees on the two sides reaches a perfect edge drop effect, and the influence from strip steel off-tracking on the edge drop is compensated.
Owner:ANGANG STEEL CO LTD

Rapid detection kit of nitrite nitrogen in aquiculture water and detection method using same

The invention relates to a rapid detection kit of nitrite nitrogen in aquiculture water. The kit internally comprises one 10 mL stoppered cuvette, one colorimetric card, one suction pipe, two 20 mL dropping bottles, 20 mL of reagent A and 20 mL of reagent B. The detection method using the kit comprises the following specific operation steps of: (1) washing the cuvette 2-3 times with the water to be detected; (2) sampling 5 mL of water sample, adding 5 drops of the reagent A, then adding 5 drops of reagent B and uniformly shaking; and (3) covering the cuvette, lightly shaking, standing for 1-3 minutes, closing the cuvette to the blank of the colorimetric card, and comparing the color of the liquid in the cuvette with the color of the colorimetric card to obtain the content (mg/L) of nitrite nitrogen in the detected water sample. According to the invention, the content of nitrite nitrogen in the water sample to be detected is displayed by a developing agent, thus the rapid detection kit has the characteristics of being accurate, rapid, convenient, inexpensive and the like, does not need for any equipment, and adopts a counting drop method to measure; and is used for field rapid detection of water quality of aquiculture water, domestic sewage, industrial wastewater and the like, the rapid detection kit is very simple in use method.
Owner:TIANJIN SHENGJI GRP CO LTD

Double-fuel machine control system and double-fuel machine control method igniting natural gas due to micro-injection of diesel oil

The invention discloses a double-fuel machine control system and a double-fuel machine control method igniting natural gas due to micro-injection of diesel oil. The double-fuel machine control system comprises a monitoring cabinet, a unit pump control unit, a natural gas control unit and a sensor group, wherein under a pure diesel oil mode and a double-fuel mode, an electronic unit pump controls an injection mass and injection timing of the diesel oil; under the double-fuel mode, the natural gas control unit controls opening and closing of a gas valve in an intake manifold of each cylinder of the engine to control the amount of the natural gas; switchover from the pure diesel oil mode to the double-fuel mode is realized through adopting a stepped oil drop method; switchover from the double-fuel mode to the pure diesel oil mode is realized through adopting a stepped gas drop method; the non-uniformity of the exhaust temperature of each cylinder is controlled through adopting an exhaust temperature correction algorithm; and the ignition timing in each working condition is controlled through adopting an MAP table lookup method. The double-fuel machine control system and the double-fuel machine control method, which are disclosed by the invention, can achieve high combustion efficiency and good emission indexes.
Owner:CHONGQING HONGJIANG MACHINERY

Fully automatic seedling pick-up and dropping device and seedling pick-up and dropping method thereof

The invention discloses a fully automatic seedling pick-up and dropping device and a seedling pick-up and dropping method thereof. The fully automatic seedling pick-up and dropping device comprises a fixed frame, a disk feeding device, a seedling ejecting device, a seedling pick-up device, a bearing device and a control system; the disk feeding device is fixed on the fixed frame and is provided with a seedling disk work position and a seedling disk waiting position relative to the fixed frame; the seedling ejecting device is mounted on the fixed frame and is arranged below a seedling disk, located at the seedling disk work position, of the disk feeding device; the seedling pick-up device is mounted on the fixed frame relative to the seedling ejecting device and is arranged above the seedling disk, located at the seedling disk work position, of the disk feeding device; the bearing device is mounted on the fixed frame relative to the seedling pick-up device and is arranged below the seedling pick-up device; the control system is mounted on the fixed frame, connected with the disk feeding device, the seedling ejecting device, the seedling pick-up device and the bearing device and used for controlling the disk feeding device, the disk ejecting device, the seedling pick-up device and the bearing device to complete seedling pick-up and dropping operations according to the timing sequence required order. The invention also discloses the seedling pick-up and dropping method of the device.
Owner:MODERN AGRI EQUIP
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