The invention relates to a rapid detection kit of nitrite nitrogen in aquiculture water. The kit internally comprises one 10 mL stoppered cuvette, one colorimetric card, one suction pipe, two 20 mL dropping bottles, 20 mL of reagent A and 20 mL of reagent B. The detection method using the kit comprises the following specific operation steps of: (1) washing the cuvette 2-3 times with the water to be detected; (2) sampling 5 mL of water sample, adding 5 drops of the reagent A, then adding 5 drops of reagent B and uniformly shaking; and (3) covering the cuvette, lightly shaking, standing for 1-3 minutes, closing the cuvette to the blank of the colorimetric card, and comparing the color of the liquid in the cuvette with the color of the colorimetric card to obtain the content (mg/L) of nitrite nitrogen in the detected water sample. According to the invention, the content of nitrite nitrogen in the water sample to be detected is displayed by a developing agent, thus the rapid detection kit has the characteristics of being accurate, rapid, convenient, inexpensive and the like, does not need for any equipment, and adopts a counting drop method to measure; and is used for field rapid detection of water quality of aquiculture water, domestic sewage, industrial wastewater and the like, the rapid detection kit is very simple in use method.