Alginate/nano-clay composite microcarrier and preparing method and device

A technology of nano-clay and alginate, which is applied in prosthetics, medical science, etc., can solve the problems of incapable of endowing microcarriers with slow-release drug mechanical properties and high sterilization requirements, and achieve strong mechanical properties, improved mechanical properties, and better The effect of applying the foreground

Active Publication Date: 2019-04-19
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is simple and easy to implement, but it fails to overcome the shortcomings that alginate materials cannot release growth factors, drugs or other biologically active ingredients for a long time, and the sterilization requirements of biological materials in the existing reaction system are relatively high
[0008] To sum up, the existing patented technology methods at home and abroad are to use various cell scaffold materials combined with cells to make microcarriers, but cannot endow microcarriers with better drug sustained release ability and better mechanical properties.

Method used

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  • Alginate/nano-clay composite microcarrier and preparing method and device
  • Alginate/nano-clay composite microcarrier and preparing method and device
  • Alginate/nano-clay composite microcarrier and preparing method and device

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Effect test

Embodiment 1

[0054] Alginate / nano-clay composite microcarriers include alginate solution and nano-clay sol, the volume ratio of alginate solution and nano-clay sol is 1:1, alginate mainly exists in the form of calcium alginate, in which Ca 2+ The mass fraction is 10%. The carrier is spherical, with a particle size of 100 μm to 600 μm. The carrier is formed by flowing in a high-voltage electrostatic field of 5kV to 20kV.

[0055] The mass percentage concentration of sodium alginate solution is 1%; the mass percentage concentration of nanoclay sol is 0.1%; Ca 2+ The solution is CaCl 2 solution, the molar concentration of the solution is 0.05mol / L.

[0056] Alginate is sodium alginate or potassium alginate, soluble in water at 1% mass fraction at 25°C, its viscosity range is 4-12cP, and its purity should be higher than 99%; nanoclay material is a natural One or several mixtures of various layered silicate materials extracted or artificially synthesized, the purity of each component should...

Embodiment 2

[0065] Alginate / nano-clay composite microcarriers include alginate solution and nano-clay sol, the volume ratio of alginate solution and nano-clay sol is 1:1, alginate mainly exists in the form of calcium alginate, in which Ca 2+ The mass fraction is 13%. The carrier is spherical, with a particle size of 200 μm to 400 μm. The carrier is formed by flowing in a high-voltage electrostatic field of 20kV.

[0066] Carriers also include cells, which are adherent cells.

[0067] The mass percentage concentration of sodium alginate solution is 3%; the mass percentage concentration of nanoclay sol is 3%; Ca 2+ The solution is CaCl 2 solution, the molar concentration of the solution is 0.3mol / L.

[0068] Alginate is sodium alginate or potassium alginate, soluble in water at 1% mass fraction at 25°C, its viscosity range is 4-12cP, and the purity is higher than 99%; the nano-clay material is nano-montmorillonite (MMT), There are three kinds of nano-saponite or nano-lithium magnesium ...

Embodiment 3

[0077] Alginate / nano-clay composite microcarriers include alginate solution and nano-clay sol, the volume ratio of alginate solution and nano-clay sol is 1:1, alginate mainly exists in the form of calcium alginate, in which Ca 2+ The mass fraction is 12%. The carrier is spherical, with a particle size of 200 μm to 400 μm. The carrier is formed by flowing in a high-voltage electrostatic field of 12kV.

[0078] Carriers also include drugs and cells to be sustained release; drugs to be sustained release are biologically active factors or drug molecules, including growth factors such as various polypeptides and proteins, or small molecule drugs or combinations thereof, preferably factors with a molecular weight of less than 80kD to achieve better Sustained release effect; the cells are stem cells, such as bone marrow mesenchymal stem cells or dental pulp stem cells, through the method of transplanting stem cells to achieve the purpose of tissue regeneration.

[0079] The mass pe...

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Abstract

The invention belongs to the technical field of tissue engineering biological scaffold materials and manufacturing thereof, and discloses an alginate/nano-clay composite microcarrier and a preparing method and device. The method comprises the steps of firstly, preparing a sodium alginate solution and nano-clay sol separately, and obtaining sodium alginate/nano-clay nano-composite hydrogel after the sodium alginate solution and the nano-clay sol are uniformly mixed. Furthermore, a stem cell suspension is mixed in the hydrogel, and besides, bioactive factors or drug ingredients can also be added. Then, the electrostatic drop method microcarrier preparing device which is mainly composed of a high-voltage power supply and a micro-injection pump is adopted, an open glass container containing acalcium chloride solution is used as a receiving device of the microcarrier, and proper injection speed and voltage are set. Compared with a pure alginate microcarrier, the prepared alginate/nano-claycomposite microcarrier has better mechanical performance, has the sustained drug release capability with which the alginate microcarrier is not endowed, and has an excellent application prospect in the field of stem cell treatment and tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering bio-scaffold material and its manufacture, and relates to an alginate / nano-clay composite microcarrier, a preparation method and a device. Background technique [0002] In the 1980s, the concept of "tissue engineering" emerged, which is a revolutionary treatment for tissue and organ damage. In the field of "tissue engineering", one of the most concerned issues is how to obtain a sufficient number of seed cells of the original phenotype, and how to prepare a biological scaffold material with excellent performance, so that the scaffold material can be loaded with biologically active components such as various types of cells Factors, and make it slow release for a long time, to give full play to its effectiveness. Cells, scaffold materials and biologically active factors can be regarded as seeds, soil and fertilizer respectively, and the synergistic effect of the three can achieve the opt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/02A61L27/20A61L27/38A61L27/54
CPCA61L27/025A61L27/20A61L27/38A61L27/3834A61L27/54A61L2300/602C08L5/04
Inventor 田卫东谢利张锐涛
Owner SICHUAN UNIV
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