44 results about "Dental pulp stem cells" patented technology

The utility model relates to the technical field of dental pulp stem cell kits, and discloses a novel dental pulp stem cell kit convenient to transport, which comprises a placing box, one side of the placing box is in threaded connection with an adaptive pressing component, the surface of the placing box is fixedly connected with an elastic sheet, two sides of the placing box are provided with fitting binding components, and the elastic sheet is fixedly connected with the elastic sheet. The adaptive abutting assembly comprises a threaded abutting column, a bearing, an abutting piece and a rubber attaching piece. The attaching binding assembly comprises a fixing sleeve, a torque spring, a rotating column and a binding attaching piece. According to the novel dental pulp stem cell kit convenient to transport, when a cell collection bottle is placed in a placement box, four binding attachment pieces are driven to be automatically attached to the surface of the cell collection bottle under the action of a torque spring, so that the device can adapt to cell collection bottles in different shapes for fixation, and the operation is convenient. Therefore, the stability of the dental pulp stem cell kit is improved, and the dental pulp stem cell kit is convenient to transport.

To provide safe and high-performance stem cells appropriate for clinical application, and a method for producing the stem cells.SOLUTION: Provided are a stem cell population derived from human deciduous tooth dental pulp, wherein 90% or more of the stem cell population is characterized by being CD117-negative, CD73-positive, CD90-positive, and CD105-positive, and a method for producing a stem cell population derived from human deciduous tooth dental pulp, the method comprising a step of culturing cells isolated from human deciduous tooth dental pulp in a medium that does not contain FBS (fetal bovine serum) in the presence of human platelet lysate (hPL).SELECTED DRAWING: None

The invention relates to the technical field of biological medicine, and provides application of a dental pulp stem cell exosome in preparation of a medicine for treating and preventing osteoarthritis, a preparation method of the dental pulp stem cell exosome comprises the following steps: S1, obtaining dental pulp tissue from a dental pulp cavity of an in-vitro tooth; s2, pretreating dental pulp tissues, adding digestive juice, digesting, centrifuging, discarding supernatant, adding a complete culture medium containing antibiotics into precipitates, mixing, filtering and centrifuging to obtain dental pulp stem cells; and S3, culturing the dental pulp stem cells by using a complete culture medium, when the confluence degree of the dental pulp stem cells reaches 60-80%, replacing the serum-free culture medium for continuous culture, collecting the dental pulp stem cell conditioned culture medium, and performing differential centrifugation to obtain the dental pulp stem cell exosome. According to the technical scheme, the regulating effect of the dental pulp stem cell exosome on endoplasmic reticulum stress and the mechanism of the dental pulp stem cell exosome for improving cartilage cell degeneration are explored.

The invention relates to the technical field of biology, in particular to an SERS probe for Mfn2 detection, a preparation method of the SERS probe, an SERS sensor and application of the SERS sensor. Based on a surface enhanced Raman spectroscopy (SERS) technology, a gold nanoparticle SERS probe coated with an antibody 1 and a silver nanoparticle SERS probe coated with an antibody 2 are constructed, a sandwich structure is formed through combination of the antibody 1, an antigen and the antibody 2, the structure effectively improves SERS hot spot distribution and Mfn2 detection sensitivity, and traditional measurement steps are simplified. The constructed biologically coded SERS probe can be used for measuring Mfn2 with different concentrations. Based on the prepared SERS sensor, the dynamic measurement of the expression of the single living cell Mfn2 in the process of electrical stimulation of dental pulp stem cell odontogenic differentiation is realized, and the SERS sensor has wide clinical application prospect and transformation value in the aspects of measurement of protein molecules in a single cell organelle and diagnosis of Mfn2 related diseases.

A pulp extraction device for obtaining clean dental pulp stem cells and a control method thereof. The present invention belongs to the technical field of stem cell microtissue extraction, and specifically relates to a pulp extraction device for obtaining clean dental pulp stem cells and a control method thereof. The present invention aims to automatically and accurately cut and extract tooth roots without human and other factors under the premise of ensuring cleanliness. It comprises a pulp extraction tool, a dental pulp stem cell extractor, a dental pulp delivery window, a dental pulp stem cell extraction tube, a dental pulp stem cell extraction tube carrier, a tooth body carrier, a motor, a high-efficiency filter, a tooth body clamp screw, a tooth body universal control assembly, a tooth body clamp, a tooth body clamp, a forward X-ray machine, a lateral X-ray machine, an extraction device main body, a compressor cooling fan and a compressor. Method: the pulp extraction tool is matched with the dental pulp stem cell extractor to quickly extract dental pulp stem cells, and a biological medical standard system is realized. The present invention is used for extracting dental pulp stem cells.

This invention provides a treatment system for promoting dental pulp repair using oral stem cells, belonging to the field of oral treatment technology. The system utilizes a dynamic gene regulation module, an adaptive intelligent scaffold, and a bio-digital fusion interface in synergy. Through the dynamic gene regulation module and the adaptive intelligent scaffold, the efficiency and therapeutic effect of dental pulp regeneration are significantly improved. The dynamic gene regulation module employs a light-controlled CRISPR system to perform real-time gene editing on dental pulp stem cells, dynamically adjusting according to the needs of the regeneration stage. The adaptive intelligent scaffold, based on 3D printing technology and manufactured using a digital model of the patient's teeth, can dynamically release growth factors and optimize the microenvironment. This combination of personalized design and dynamic regulation allows for adaptive adjustments to meet the specific needs of the patient, not only improving the repair efficiency of dental pulp tissue but also enhancing the success rate of functional reconstruction. This provides patients with dental pulp injury with a more efficient and precise treatment option, promoting the personalized development of oral regenerative medicine.

Provided is a novel cancer microenvironment improving agent capable of improving a cancer microenvironment by microparticles derived from dental pulp-derived stem cells.SOLUTION: It is preferable that the agent is an agent for improving a cancer microenvironment containing microparticles derived from dental pulp-derived stem cells, an agent for improving an antitumor immune response, it is more preferable that the agent improves the cancer microenvironment in a direction in which proliferation of cancer cells can be suppressed by inhibiting the activity of CAF, and it is particularly preferable that the agent improves the cancer microenvironment in a direction in which proliferation of cancer cells can be suppressed by inducing M2-type macrophages into M1-type macrophages, and it is further preferable that the agent is a prophylactic or therapeutic agent for an antitumor immune response, or a method for improving a cancer microenvironment.SELECTED DRAWING: Figure 6

This invention proposes a method for preparing dental pulp stem cell exosomes and their applications, belonging to the field of exosome technology. Dental pulp stem cells from exfoliated deciduous teeth are seeded into serum-free α-MEM medium containing microparticles, insulin-like growth factor, transforming growth factor-β1, penicillin, and streptomycin for culture. Exosomes are isolated, dispersed in PBS solution, and incubated with microparticles, propylene glycol, and rhodioloside to obtain highly active dental pulp stem cell exosomes. The surface of these exosomes is then modified with shikonin and interferon to obtain dental pulp stem cell exosomes. The dental pulp stem cell exosomes prepared by this invention exhibit good affinity for titanium implant-mediated osteointegration repair materials, demonstrating good anti-inflammatory, immunomodulatory, osteogenic, and damage-reducing effects. Furthermore, the preparation method is simple, yields high output, and operates under mild conditions, showing broad application prospects.

The invention provides a dental pulp stem cell exosome as well as a preparation method and application thereof, and belongs to the technical field of exosomes. The method comprises the following steps: inoculating exfoliated deciduous tooth pulp stem cells into a serum-free alpha-MEM culture medium containing microparticles, insulin-like growth factors, transforming growth factor-beta1, penicillin and streptomycin for culturing, separating exosomes, dispersing the exosomes into a PBS solution, adding microparticles, propylene glycol and salidroside for incubation, and separating to obtain the high-activity pulp stem cell exosomes. And performing surface modification on alkannic acid and interferon to prepare the dental pulp stem cell exosome. The dental pulp stem cell exosome prepared by the invention has relatively good affinity to a titanium implant material mediated osseointegration repair material, has relatively good advantages of resisting inflammation, regulating immunity, promoting osteogenesis, reducing injury and the like, is simple in preparation method, relatively high in yield and mild in condition, and has a wide application prospect.

The present application provides a kind of dentin inducer for enhancing histone succinylation and its application, wherein the dentin inducer is dimethyl succinate.The present application finds that Wnt3a protein with clear odontogenic differentiation effect promotes odontogenic differentiation of dental pulp stem cells by increasing intracellular succinic acid content to induce histone succinic acid, and direct addition of succinic acid or succinic acid derivatives such as dimethyl succinate can play a Wnt3a replacement role, enhance stem cell histone succinylation and promote odontogenic differentiation of dental pulp stem cells.Furthermore, compared with Wnt3a protein class inducer, succinic acid or succinic acid derivative has the advantages of stable preparation and clear target, and is more suitable for clinical operation, which has important practical significance for promoting the clinical transformation of dental pulp dentin complex regeneration technology.

The invention discloses a personalized dental pulp separation guide plate based on digital space geometric positioning and a manufacturing method thereof, and relates to the field of digital oral cavities and oral cavity dental pulp stem cells, and the manufacturing method comprises the following steps: performing digital acquisition on intraoral data of a target dentition of a subject to obtain an intraoral three-dimensional scanning digital model; the method comprises the following steps: collecting an oral cavity CBCT image of a subject, and establishing a model of target teeth; a personalized dental pulp separation guide plate and a tooth fixing device are designed by utilizing a spatial geometric positioning principle; the 3D printer is used for printing the personalized dental pulp separation guide plate meeting the design requirement, the personalized dental pulp separation guide plate is fixed to a to-be-treated tooth of a subject through the tooth fixing device, and dental pulp tissue is separated through high-speed dental handpiece equipment. According to the invention, an operator can be guided to cut teeth at a certain distance and at a fixed position, so that the damage to dental pulp tissues is avoided, the accuracy and safety of operation are ensured, and the damage to dental pulp stem cell tissues is avoided.

The invention provides a dental pulp stem cell exosome-loaded acellular tissue engineering nerve conduit as well as a preparation method and application thereof, relates to the technical field of biomedical engineering and nerve regeneration medicine, and aims to solve the problems of poor mechanical property, low bionic degree, high degradation rate, insufficient biological activity and the like of an existing nerve conduit. The technical key point of the invention is as follows: the dental pulp stem cell exosome-loaded acellular tissue engineering nerve conduit is provided, and the nerve conduit is obtained by loading a nanofiber conduit with enhanced function of over-expressing let-7i-5p, the porous nanofiber catheter is a porous catheter which is obtained after a polyvinyl alcohol (PVA)-chitosan (CS)-graphene (G) electrostatic spinning membrane is subjected to pipe reeling and crosslinking, and the nucleotide sequence of let-7i-5p in DPSC-Exo is shown as SEQ NO.1. The invention further discloses a preparation method of the porous nanofiber catheter.

The invention provides a mechanical and biochemical coupling regulation and control method and a stem cell spatial differentiation system, and relates to the technical field of regenerative medicine and tissue repair, the mechanical and biochemical coupling regulation and control method comprises the following steps: S1, applying 1 Hz + / -0.2 Hz and 10% + / -0.5% periodic compression strain, activating YAP / TAZ nuclear transposition through an integrin beta1-FAK pathway, the nuclear localization proportion of YAP / TAZ being greater than 65%; s2, triggering a BMP-2 secretion gradient when the YAP nuclear localization proportion is greater than 70%, and S3, establishing a bidirectional biochemical gradient through a microfluidic system: pouring TGF-beta1 into a core region to be 20ng / mL, pouring dexamethasone into a marginal region to be 50ng / mL, and adding SDF-1alpha into the marginal region to be 50ng / mL. Preferably, the chip of the micro-fluidic gradient module is designed into a tree-shaped fractal channel, and the gradient half-decay distance is 300 microns. Preferably, the inoculation density of the dental pulp stem cells is 5 * 10 cells / cm < 2 >. According to the invention, the YAP / TAZ pathway is regulated and controlled through mechanical-biochemical coupling, the spatial differentiation of stem cells is realized, the resolution ratio reaches 200 microns, the construction period is shortened by 50%, and the transplantation success rate is greater than 90%.

This invention relates to the field of cell regulation technology and discloses a multi-parameter feedback-based intelligent regulation method for Chir99021-induced differentiation of dental pulp stem cells. The method includes: acquiring a dataset of the differentiation process of dental pulp stem cells and performing structured analysis to obtain a multi-parameter monitoring dataset; performing multi-dimensional assessment of cell differentiation status to obtain a differentiation status assessment characterization; obtaining drug release regulation parameters based on the differentiation status assessment characterization and historical differentiation trend data; performing multi-dimensional planning on the differentiation status assessment characterization and drug release regulation parameters to obtain a regulation strategy, and encoding and reconstructing the regulation strategy to obtain microenvironment regulation instructions; applying the microenvironment regulation instructions to obtain an optimized culture microenvironment and monitoring cell state information in the optimized culture microenvironment; and iteratively regulating the drug release regulation parameters based on the cell state information to obtain the final regulation instructions. This invention can improve the accuracy of Chir99021-induced differentiation regulation of dental pulp stem cells.

The utility model relates to the technical field of dental pulp stem cell separation, and discloses a dental pulp stem cell separation and extraction device which comprises a device shell, a driving shaft is installed in the device shell, and a stepping motor fixedly connected with the device shell is fixed to the lower end of the driving shaft. A balance mechanism used for balancing the device is arranged on the outer surface of the driving shaft, a fixing mechanism used for fixing materials is arranged at the upper end of the driving shaft, and a protection mechanism used for protection is arranged on one side of the device shell. The balance mechanism comprises an adjusting piece. Through the arrangement of the balance mechanism, the lead screw can be driven to rotate through the adjusting motor arranged in the adjusting piece, the horizontal position of the movable block can be adjusted under the cooperation of the guide rod, then the position of the balancing weight is adjusted, the centrifugal force generated when the driving shaft rotates can be stabilized, and then the shaking situation during centrifugation is reduced; and the stability of the device is improved.

The invention provides a preparation method and application of a dental pulp stem cell preparation based on AI activity prediction, and the method comprises the following steps: collecting historical preparation parameters and corresponding activity indexes of the dental pulp stem cell preparation, constructing a historical database, and carrying out data preprocessing and core parameter screening; according to the method, an activity prediction model comprising a first sub-model and a second sub-model is constructed, bidirectional function prediction is realized through two-stage training, multiple types of activity indexes and comprehensive scores can be predicted based on preparation parameters, and the preparation parameters can be reversely recommended according to activity requirements; and preparing a compound preparation which takes the dental pulp stem cells as a core and PDD-ALN as a synergistic enhancement carrier based on model output. The method can accurately guide the preparation of the dental pulp stem cell preparation, and is especially suitable for the development of periodontitis treatment drugs.

The utility model discloses an efficient dental pulp stem cell extraction device which comprises a box body, a driving assembly is installed on the top of an upper supporting plate, a sliding assembly is installed on the top of a lower supporting plate, a positioning assembly is installed in the sliding assembly, and an extraction assembly is installed in the box body. According to the efficient extraction device for the dental pulp stem cells, a driving motor is started to drive a driving shaft to enable a driving gear to rotate, the driving gear drives a rack to enable a rotating block to rotate outside a guide rail, and the rotating block rotates to enable an internal sliding block to drive a connecting column to slide under limitation of a straight notch of an arc notch; a connecting column drives a positioning block to enable an anti-skid plate to make contact with and fix teeth, a spindle motor is started to drive an inner-tooth saw blade to rotate, then an air cylinder is started to drive a fixing plate to enable the inner-tooth saw blade to cut and collect the teeth, the teeth are conveniently fixed, and it is prevented that the teeth slip to affect follow-up collection.

The application discloses a PLGA microsphere-PCL / GelMA composite scaffold based on nerve growth factor (NGF) sustained release and a preparation method and application thereof. The composite scaffold is constructed by embedding the NGF-loaded PLGA microsphere into the PCL framework and the GelMA hydrogel matrix, and a three-phase composite system with mechanical support, sustained release of biological factors and cell adhesion function is constructed. In vitro experiments show that the NGF-loaded composite scaffold significantly promotes the osteogenic differentiation of dental pulp stem cells, and the alkaline phosphatase activity is improved, the formation of mineralized nodules is increased, and the expression of osteogenesis-related genes RUNX2 , ALPL , COL1A1 and BGLAP is up-regulated. When applied to a rat skull critical defect model in vivo, the composite scaffold significantly promotes new bone formation and vascular nerve regeneration. Mechanism research shows that it realizes the regulation of osteogenesis and bone resorption by activating the PI3K / AKT signaling pathway. The composite scaffold is suitable for the repair of complex bone defects in the region rich in nerves and blood vessels, and has good clinical application prospect.

The invention provides a pulp capping material comprising bioactive glass fibers, mesoporous tricalcium silicate, and zirconia dioxide. The initial setting time of this pulp capping material can be reduced to 7 minutes. The material exhibits no significant cytotoxicity and can significantly enhance cell viability. Additionally, it increases the expression of alkaline phosphatase and Dentin Sialophosphoprotein mineralization genes in dental pulp stem cells.

The invention provides an application of a small molecule drug for promoting mitochondrial fusion in preparation of a product for promoting tooth differentiation. The small molecule drug for promoting mitochondrial fusion is a mitochondrial fusion promoter M1. The invention firstly verifies that the mitochondrial fusion promoter M1 is similar to Wnt3a in protein induction effect and can induce dental pulp stem cell odontogenic differentiation, and further defines that the mitochondrial function homeostasis maintained by the mitochondrial fusion promoter M1 is one of the key action mechanism and target spot of odontogenic differentiation. Compared with a Wnt3a protein, the mitochondrial fusion promoter M1 serving as a small molecule drug has the advantages of being stable in preparation and clear in target spot compared with a protein preparation, and is more suitable for clinical operation.

The invention relates to the technical field of biology, in particular to a medicine and application thereof. The drug is an exosome (DPSCs-Exos) derived from dental pulp stem cells (DPSCs-Exos). The DPSCs-Exos is given after acute cerebral apoplexy, so that sensory movement and cognitive function recovery after cerebral ischemia can be promoted. The DPSCs-Exos can improve ischemia for 45 minutes, and brain tissue injury and neurological function injury of animals after reperfusion for 28 days. The DPSCs-Exos promotes the expression increase of protein arginine methyltransferase (PRMT5) in oligodendroglia precursor cells (OPC), inhibits the expression of a differentiation inhibition factor 2 (ID2) through nucleation, promotes the OPCs to proliferate and differentiate into oligodendroglia cells (OL), and promotes the integrity repair of white matter. In addition, DPSCs-Exos can promote proliferation and migration of vascular endothelial cells and promote blood brain barrier repair.

Proposed are a method of preparing new biomaterials for dental pulp regeneration and a composition of new biomaterials prepared accordingly. The composition of new biomaterials prepared according to the method of preparing new biomaterials for dental pulp regeneration by mixing a first precursor containing calcium silicate-based cement (CSC) at an appropriate concentration and a second precursor containing a secretome of dental pulp-derived mesenchymal stem cells in an appropriate ratio and then drying the resulting mixture under appropriate drying conditions is characterized by further improving the osteogenic differentiation ability of dental pulp stem cells compared to existing materials while maintaining the microhardness of the composition to an appropriate standard. In addition, the composition is advantageous in that an aesthetic effect is excellent by not causing tooth discoloration.

The application discloses a novel dental nanocomposite material and a preparation method and application thereof, and the nanocomposite material comprises the following preparation raw materials in parts by weight: 15-30 parts of eucommia ulmoides derived antibacterial carbon dots-epigallocatechin gallate, 10-25 parts of iron nitrate, 5-7 parts of boric acid, 8-12 parts of metformin hydrochloride, 4-6.6 parts of zinc chloride, 40-60 parts of hyaluronic acid and 4-6 parts of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid sodium. The application takes eucommia ulmoides derived antibacterial carbon dots-epigallocatechin gallate as a core, and through the o-benzenetriol structure of epigallocatechin gallate, a stable metal-phenol / boric acid ester hybrid network inner shell is cooperatively constructed with iron ions and boric acid, and the effect of synergistically improving anti-inflammatory and long-acting promotion of directional differentiation of dental pulp stem cells is achieved.

This invention relates to the field of stem cell isolation, and discloses a dental pulp stem cell isolation reactor and a method for efficient exosome extraction. The reactor includes a controller, a protective shell and a motor mounted on the inner wall of the controller, a rotating frame fixedly connected to the output end of the motor, a test tube slidably connected to the bottom end of the placement frame, a fixing component on the inner wall of the placement frame, toothed rings fixedly connected to both sides of the placement frame, a movable frame threadedly connected to the outer wall of the threaded rod, a rack fixedly connected to the bottom end of the movable frame, a synchronous belt meshing with the toothed end of a first synchronous wheel, and a second synchronous wheel fixedly connected to one end of the rotating wheel. By placing a test tube containing dental pulp stem cells into the placement frame and fixing it, rotating the rotating wheel causes the placement frame to rotate within the rotating frame via a transmission component to adjust the angle of the test tube. After covering with a movable cap, the controller controls the motor to drive the rotating frame to rotate, thereby changing the centrifugal force on the stem cells.

The invention provides a separation and preparation method of dental pulp stem cells. The method comprises the following steps: using dental stem cells (DSC) separated from dental pulp tissues of human permanent teeth or deciduous teeth; obtaining a single-cell suspension through a double-enzyme digestion method; washing the single-cell suspension and the phosphoric acid buffer salt solution with the same volume for three times; carrying out adherent culture on the undigested tissues; culturing in an improved MEM culture medium (MEM-alpha) supplemented with fetal calf serum (FBS) with the volume fraction of 20%, penicillin with the volume fraction of 100 g / ml and streptomycin with the volume fraction of 100 g / ml in an incubator at the temperature of 37 DEG C under the condition of 5% CO2; according to the double-enzyme digestion method, the culture medium is replaced every two days; the culture medium is replaced once every 4-5 days by the tissue adherence method; after 90% confluence is achieved, performing trypsin digestion to harvest adherent cells; under the condition, the content of CD117 positive cells exceeds 96.67%. The dental pulp stem cells obtained by the method do not induce premature differentiation of cells, the cell proliferation speed is greatly increased, more sufficient nutrition is provided for the cells, synthesis of protein is promoted, proliferation of the cells is promoted, and differentiation of the cells is inhibited; the separation speed and the separation effect are improved, and the operation difficulty is also reduced. By utilizing the method disclosed by the invention, a rapid and stable separation method and an efficient amplification system can be established, and the method has a very wide clinical application prospect in the fields of life science and medicine. Along with continuous development of research, hDPSCs can play a greater role in the medical field.

The invention discloses a guided dental pulp stem cell extracellular matrix nerve scaffold and a preparation method thereof, and belongs to the technical field of biological materials. The method comprises the following steps: culturing dental pulp stem cells, stimulating the dental pulp stem cells to secrete an extracellular matrix, and carrying out decellularization treatment to obtain DPSC-dECM; the preparation method comprises the following steps: preparing the DPSC-ECM into a suspension, mixing the suspension with a PLCL solution, spinning by adopting an electrostatic spinning technology to form an oriented DPSC-ECM membrane, rolling to form a catheter inner layer, spinning a PLCL fiber layer on an outer layer, and drying to obtain the double-layer tubular nerve stent. The inner layer of the stent is composed of DPSC-ECM / PLCL composite oriented nanofibers, and can provide active biological signals and oriented physical guidance at the same time, effectively maintain Schwann cell repair phenotypes, significantly enhance cell adhesion, proliferation, oriented migration, autophagy and secretion functions, and promote angiogenesis at the same time, thereby achieving good axon regeneration; the outer layer PLCL fiber provides mechanical support; the problems that a traditional nerve repair material is difficult to maintain the Schwann cell repair function and is insufficient in guidance are solved, and a new strategy is provided for peripheral nerve defect repair.

The invention discloses selenium-containing bioglass, preparation thereof and application of the selenium-containing bioglass in a pulp covering material for repairing dental pulp injury, or application of the selenium-containing bioglass in expression promoters of RUNX transcription factor 2, dentin saliva phosphoprotein and dentin matrix acid phosphoprotein 1. The preparation method comprises the following steps: preparing Se-Ca-Si-P quaternary system sol by adopting a sol-gel method, sealing and aging at room temperature until the sol is gel-shaped, and drying; and grinding the dried product, calcining, carrying out secondary grinding on the calcined powder, and screening to obtain the selenium-containing bioglass. The material not only can rapidly induce bone-like apatite deposition, has excellent antioxidant and antibacterial activity, but also can remarkably promote proliferation and migration of dental pulp stem cells, effectively up-regulate expression of a dental differentiation gene RUNX transcription factor 2, dentin saliva phosphoprotein and dentin matrix acid phosphoprotein 1, and can promote the growth of dental pulp stem cells. The material can be used as a pulp capping material in the aspects of dental pulp injury repair, dentin regeneration and the like.

The invention relates to the technical field of medical reagent preparation, in particular to application of dental pulp stem cell concentrated supernate. Application of dental pulp stem cell concentrated supernatant as preserving fluid in biological field or application of dental pulp stem cell concentrated supernatant as preserving fluid in bone regeneration field or application of dental pulp stem cell concentrated supernatant as preserving fluid and deep hypothermia liquid nitrogen (-196 DEG C) in bone regeneration field or application of dental pulp stem cell concentrated supernatant as preserving fluid and deep hypothermia liquid nitrogen (-196 DEG C) and preserving the skull flap at-196 DEG C by using the liquid as preserving liquid and combining profound hypothermia liquid nitrogen. The dental pulp stem cell concentrated supernatant has good proliferation promoting ability, osteogenesis ability and angiogenesis ability, is safe as a preserving fluid component, has anti-inflammatory activity for adjuvant therapy, has good biocompatibility, and plays the dual roles of osteogenesis and angiogenesis. And inflammation regulation and control of the microenvironment are facilitated, bone regeneration is promoted, and the application value is important.

The present invention is directed to therapeutic multifunctional immature dental pulp stem cells (IDPSCs), and IDPSCs multi-lineage compositions. The invention is further directed to the use of IDPSCs and compositions to reduce the risk of and / or treat degenerative diseases or for other medicinal and aesthetic purposes.

The application discloses application of all-trans retinoic acid in preparation of a drug for hemangiogenic differentiation of dental pulp stem cells, wherein the hemangiogenic differentiation comprises at least one of the following cases: at least one of the following is increased: tubule length, connection point number, blood vessel lumen volume and lumen number; blood vessel extension length is increased or sprouting is increased; blood vessel formation speed is accelerated; blood vessel density is increased; and expression amount of a blood vessel regulation related gene or amount of a related protein is increased. The application finds that a retinoic acid signal channel is a candidate target point for promoting hemangiogenic differentiation of the dental pulp stem cells in a starvation environment and can be applied to dental pulp regeneration treatment, and therefore, the application has a wide application prospect in the field of the dental pulp regeneration treatment.