6 results about "Dental pulp stem cells" patented technology

This invention provides a treatment system for promoting dental pulp repair using oral stem cells, belonging to the field of oral treatment technology. The system utilizes a dynamic gene regulation module, an adaptive intelligent scaffold, and a bio-digital fusion interface in synergy. Through the dynamic gene regulation module and the adaptive intelligent scaffold, the efficiency and therapeutic effect of dental pulp regeneration are significantly improved. The dynamic gene regulation module employs a light-controlled CRISPR system to perform real-time gene editing on dental pulp stem cells, dynamically adjusting according to the needs of the regeneration stage. The adaptive intelligent scaffold, based on 3D printing technology and manufactured using a digital model of the patient's teeth, can dynamically release growth factors and optimize the microenvironment. This combination of personalized design and dynamic regulation allows for adaptive adjustments to meet the specific needs of the patient, not only improving the repair efficiency of dental pulp tissue but also enhancing the success rate of functional reconstruction. This provides patients with dental pulp injury with a more efficient and precise treatment option, promoting the personalized development of oral regenerative medicine.

This invention relates to the field of cell regulation technology and discloses a multi-parameter feedback-based intelligent regulation method for Chir99021-induced differentiation of dental pulp stem cells. The method includes: acquiring a dataset of the differentiation process of dental pulp stem cells and performing structured analysis to obtain a multi-parameter monitoring dataset; performing multi-dimensional assessment of cell differentiation status to obtain a differentiation status assessment characterization; obtaining drug release regulation parameters based on the differentiation status assessment characterization and historical differentiation trend data; performing multi-dimensional planning on the differentiation status assessment characterization and drug release regulation parameters to obtain a regulation strategy, and encoding and reconstructing the regulation strategy to obtain microenvironment regulation instructions; applying the microenvironment regulation instructions to obtain an optimized culture microenvironment and monitoring cell state information in the optimized culture microenvironment; and iteratively regulating the drug release regulation parameters based on the cell state information to obtain the final regulation instructions. This invention can improve the accuracy of Chir99021-induced differentiation regulation of dental pulp stem cells.

The application discloses a novel dental nanocomposite material and a preparation method and application thereof, and the nanocomposite material comprises the following preparation raw materials in parts by weight: 15-30 parts of eucommia ulmoides derived antibacterial carbon dots-epigallocatechin gallate, 10-25 parts of iron nitrate, 5-7 parts of boric acid, 8-12 parts of metformin hydrochloride, 4-6.6 parts of zinc chloride, 40-60 parts of hyaluronic acid and 4-6 parts of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid sodium. The application takes eucommia ulmoides derived antibacterial carbon dots-epigallocatechin gallate as a core, and through the o-benzenetriol structure of epigallocatechin gallate, a stable metal-phenol / boric acid ester hybrid network inner shell is cooperatively constructed with iron ions and boric acid, and the effect of synergistically improving anti-inflammatory and long-acting promotion of directional differentiation of dental pulp stem cells is achieved.

This invention relates to the field of stem cell isolation, and discloses a dental pulp stem cell isolation reactor and a method for efficient exosome extraction. The reactor includes a controller, a protective shell and a motor mounted on the inner wall of the controller, a rotating frame fixedly connected to the output end of the motor, a test tube slidably connected to the bottom end of the placement frame, a fixing component on the inner wall of the placement frame, toothed rings fixedly connected to both sides of the placement frame, a movable frame threadedly connected to the outer wall of the threaded rod, a rack fixedly connected to the bottom end of the movable frame, a synchronous belt meshing with the toothed end of a first synchronous wheel, and a second synchronous wheel fixedly connected to one end of the rotating wheel. By placing a test tube containing dental pulp stem cells into the placement frame and fixing it, rotating the rotating wheel causes the placement frame to rotate within the rotating frame via a transmission component to adjust the angle of the test tube. After covering with a movable cap, the controller controls the motor to drive the rotating frame to rotate, thereby changing the centrifugal force on the stem cells.

This invention relates to the fields of biomedicine and tissue engineering, and discloses a DNA hydrogel-dental pulp stem cell complex, its preparation method, and its application in the preparation of drugs for spinal cord injury repair. The DNA hydrogel comprises multiple DNA chains, at least one of which contains multiple Apt19s sequences. These Apt19s sequences specifically bind to the ALPL protein on the surface of dental pulp stem cells. This invention co-incubates the DNA hydrogel with dental pulp stem cells, utilizing the high affinity of aptamers and DNA self-assembly to construct a DNA hydrogel-dental pulp stem cell complex that integrates specific adhesion, anti-inflammatory, and antioxidant effects of dental pulp stem cells, thereby achieving effective repair of spinal cord injuries.

The application relates to the technical field of biological medicine, and discloses a high-activity dental pulp stem cell separation treatment device and an exosome extraction and culture method.The device comprises a storage box, a U-shaped frame, a first hydraulic cylinder, a second hydraulic cylinder, a clamping arm, a top punching assembly, a spraying assembly and a visual module.The second hydraulic cylinder drives the clamping arm to adaptively clamp a tooth sample, the top punching assembly accurately punches and cracks the tooth, the spraying assembly cleans impurities in all directions, the visual module realizes real-time monitoring of operation, and the negative pressure suction tube is used to realize nondestructive acquisition of dental pulp tissue.The exosome extraction and culture method comprises the following steps: four-enzyme synergistic digestion of stem cells, resveratrol combined with tumor necrosis factor alpha stimulation and expansion, multi-stage centrifugation combined with tangential flow filtration purification and the like.The application solves the problems of easy damage and impurity residue of artificial acquisition of dental pulp tissue, improves the activity and proliferation capacity of stem cells, optimizes the yield, purity and biological activity of exosomes, and provides technical support for the biological medicine application of stem cells and exosomes.