175 results about "Dental pulp stem cells" patented technology

The present invention provides a culture of isolated adult human dental pulp stem cells that can differentiate into dentin / pulp tissue that can be used to produce a tooth in a human being. The present invention further provides a method of regenerating human dentin / pulp tissue.

The object is to provide a novel use application of a dental pulp stem cell collected from a deciduous tooth or a permanent tooth. Disclosed is a composition for autotransplantation or allotransplantation, which is characterized by comprising a dental pulp stem cell collected from a deciduous tooth or a permanent tooth.

Provided is a novel and creative dental tissue regeneration method for regenerating dental tissue after pulpectomy or the enlargement and cleaning of an infected root canal. After pulpectomy or the enlargement and cleaning of an infected root canal, a root canal filler (200) having an extracellular matrix (210) containing the cells (220) enriched for dental pulp stem cells, is inserted into the apical side of the root canal of a target tooth (100). The cells including dental pulp stem cells include at least one of the following: dental pulp SP cells, CD31-negative and CD146-negative cells, CD24-positive cells, CD105-positive cells, and CD150-positive cells. For instance, dental pulp SP cells are CD31− and CD146− negative. Even if pulpitis due to deep caries occurs, appropriate dental pulp regeneration and recovery of dental pulp function are possible.

A method for constructing banks of human dental pulp stem cells comprises the steps of acquiring basic information, obtaining dental pulp stem cells, cryopreserving and putting dental pulp stem cells into cell banks and recording and checking information. When the banks of dental pulp stem cells are used, recovery and multiplication culture are carried out in accordance with the actual demands. In the method, the dental pulp stem cells are obtained from dental pulp of waste healthy human teeth and a reserve bank for effective resources is established by using system engineering. A great quantity of dental pulp stem cells with functional activities can be obtained through short-term culture of the human dental pulp stem cells stored in the banks and can be stored for long term without losing the activities. Compared with the prior art, the invention is characterized in that the construction operation specification is simple, easy to grasp, safe and feasible; the bank construction cost is low and the standardization degree is high; and the cells from different age groups adopt different cryopreservation solution so that the activities of the recovered cells are enhanced, thus expanding the sources of the cells and enhancing the activities of the cells. The teeth used in the method are those which are extracted for various reasons and are waste, thus greatly saving the medical resources; therefore, the construction has wide application prospect.

The present invention is directed to therapeutic multifunctional immature dental pulp stem cells (IDPSCs), and IDPSCs multi-lineage compositions. The invention is further directed to the use of IDPSCs and compositions to reduce the risk of and / or treat degenerative diseases or for other medicinal and aesthetic purposes.

Disclosed is a therapeutic material for cerebral infarction, which can recover a vascular disorder in an area affected by cerebral infarction to improve the brain function. The therapeutic material for cerebral infarction includes a dental pulp stem cell comprising at least one member selected from a CD105-positive cell, an SP cell, a CD24-positive cell, a CD133-positive cell, a CD271-positive cell and a CD150-positive cell. The therapeutic material may additionally include a protein secreted from a dental pulp cell. A transplanted dental pulp stem cell cannot be differentiated directly into a neural progenitor cell or a neurocyte, but is involved indirectly in the promotion of differentiation to eliminate an area affected by cerebral infarction, thereby recovering the affected area into a normal area.

The invention provides a large scale production technology for a dental pulp stem cell. The large scale production technology comprises following steps of (1) separating and extracting the dental pulp stem cell; (2) amplifying the dental pulp stem cell through two dimension cultivation; (3) detecting the dental pulp stem cell; (4) cultivating the dental pulp stem cell on a large scale; (5) freeze preserving the dental pulp stem cell; and (6) freeze preserving the dental pulp stem cell for a long term. The invention provides an in vitro large-scale cultivating and amplifying method for a dental pulp stem cell, establishes a long-term, standard and systematized freeze preserving system for a high quality high activity dental pulp stem cell, so that the method can provide a high enough quality stem cell resource for clinic and scientific researches, and has low cost and a wide application prospect.

A method for reconstructing a functional tooth by using a carrier loaded with a plurality of mesenchymal stem cells including at least one of a stem cell derived from Apical Papilla (SCAP), Periodontal Ligament Stem Cells (PDLSC), and Dental Pulp Stem Cells (DPSC). The mesenchymal stem cell loaded carrier is planted into a site within a subject's oral cavity to form a bio-root upon which a crown may be affixed, The mesenchymal stem cells will develop new periodontal tissues to stabilize the bio-root. Methods disclosed herein are particularly beneficial for subjects lacking good bone structures for conventional crown treatments.

The present invention provides a method and compositions for tooth regeneration. Both implants of adipose-derived stem cell and dental pulp stem cell are able to grow self-assembled new teeth in extraction sockets when adding BMP2. The regenerated tooth is not only structurally similar to a normal tooth, but also well-developed in vascular and nervous systems with functions of growth, communication, and sensation. They are natural living teeth derived from this invented implantation method without any engineering procedure. It is ready for clinical testing and may be applied to future dental clinics.

The invention discloses stem cells for specifically expressing a programmed death receptor 1 (PD-1), and particularly relates to the stem cells from an organic cavity. The stem cells comprise but notlimited to dental pulp stem cells, gingival mesenchymal steam cells (GMSCs), periodontal ligament stem cells (PDLSCs), stem cells of apical papilla (SCAPs) and dental follicle stem cells (DFSCs) or any combination thereof, and preferably comprise stem cells from human exfoliated deciduous teeth (SHED) and / or dental pulp mesenchymal stem cells for permanent teeth (DPSC); the stem cells also includemesenchymal stem cells from other tissues, which do not express the PD-1 at first and express the PD-1 after being modified by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), suchas PD-1 plus bone mesenchymal stem cells (BMMSC) modified by the CRISPR. The invention further discloses an identifying and separating method for the stem cells for specifically expressing the PD-1,a method for preparing the PD-1 plus mesenchymal stem cells modified by the CRISPR as well as application of the mesenchymal stem cells for expressing the PD-1, disclosed by the invention, in tissue regeneration, pain relief and treatment of chronic pain and a series of diseases.

The present invention relates to a composition for the differentiation of dental pulp stem cells into odontoblasts and an IgG or IgM type monoclonal antibody that specifically binds to the surface of odontoblasts differentiated from the stem cells. According to the present invention, BMP2 and BMP4 are optimally combined to significantly increase the differentiation efficiency of dental pulp stem cells into odontoblasts, to induce the mineralization of the matrix, and to improve the differentiation ability of odontoblasts into dentin. Further, the IgG or IgM monoclonal antibody that specifically binds to the surface of odontoblasts of the present invention is used to effectively isolate and purify odontoblasts, which can be useful for tissue regeneration and differentiation.

The invention relates to the field of biotechnology and discloses a stem cell exosome preparation, and a preparation method and application thereof. The stem cell exosome preparation disclosed by the invention comprises dental pulp stem cell exosomes, dental pulp stem cells, basic fibroblast growth factors and a solvent, wherein the dental pulp stem cell exosomes are exosomes extracted from the dental pulp stem cells; 200 microliters of the preparation contain the exosomes extracted from 1*10<6> to 1*10<7> dental pulp stem cells and 1*10<6> to 3*10<6> dental pulp stem cells; the content of the basic fibroblast growth factors is 5 to 15 ng / mL; and the solvent is normal saline. The invention also discloses the exosome preparation or application of the exosome preparation prepared by the preparation method to preparation of a biological preparation for treating gastritis. The stem cell exosome preparation disclosed by the invention has a good curative effect on gastritis.

The invention relates to the technical field of adipose-derived stem cell isolated culture, and discloses a dental pulp stem cell isolated culture method. The dental pulp stem cell isolated culture method comprises the following steps: washing adipose tissues, digesting the adipose tissues by using collagenase Type I, separating adipose stromal vascular fractions, then carrying out scavenging-precipitation, planting and culturing obtained primary cells to obtain the primary cells in the end, and carrying out primary cell passage. The dental pulp stem cell isolated culture method has the beneficial effects that by standardizing the dental pulp stem cell isolated culture method and various parameters in concrete operations, the adipose-derived stem cell isolated culture efficiency is improved, the output rate is increased, and the production cost is reduced.

The invention features methods and compositions for promoting the growth and differentiation of dental pulp stem cells and the use of the differentiated cells for the treatment of orthopedic conditions.

A method for preparing primary cell dental pulp stem construction and method of dental pulp stem cell library, the use of thermostatic shaker at specific temperature and speed conditions, can be in a few minutes after digestion, to extract enough has good biological activity of dental pulp stem cells, which can shorten the primary dental pulp stem cells generation of extraction time, save time and cost, large scale production can be obtained at the same time, plenty of pulp stem cells, can meet the requirements of dental pulp stem cells in stem cell library and later stem cells for clinical treatment and regenerative medicine research needs, and the pulp tissue under conventional semi digested 30min or full digestion 1H control group compared to the amount of DPSCs had no significant difference, but the cell proliferation activity was significantly higher than the control group. Therefore, this method can be carried out quickly in batches in the construction of DPSCs library, which shortens the time of DPSCs primary extraction, saves time and cost, and is suitable for large-scale production.

The invention relates to the field of bio-technology, and discloses a stem cell exosome preparation as well as a preparation method and an application thereof. The stem cell exosome preparation provided by the invention consists of a dental pulp stem cell exosome, dental pulp stem cells, human albumin and a solvent, wherein the dental pulp stem cell exosome is extracted from the dental pulp stem cells; every 5ml of the preparation contains 1*10<6>-1*10<7> pieces of the exosome extracted from the dental pulp stem cells; the content of the dental pulp stem cells is 5*10<6> cells / 5mL; the mass concentration of the human albumin is 2-8%; and the solvent is normal saline. The invention also discloses the preparation method of the exosome preparation and a preparation method of the dental pulp stem cell exosome. The invention also discloses an application of the exosome preparation or the exosome preparation prepared by the preparation method in preparing biological preparations for treating nephritis. The stem cell exosome preparation provided by the invention has a good curative effect on the nephritis.

The invention discloses a dopamine-hyaluronic acid coating material as well as a preparation method and application thereof in promoting specific adhesion of dental pulp stem cell to dentin, and belongs to the technical field of dental pulp regeneration. The preparation method comprises the following steps: sequentially adding raw materials including hyaluronic acid, 1-(3-dimethylamino propyl)-3-ethyl carbodiimide, N-hydroxyl succinimide and dopamine hydrochloride into deionized water according to a molar ratio of (1 to 10) to 2 to 2 to 1, and mixing uniformly; stirring at room temperature under nitrogen protection; adjusting the pH to 4-5 with hydrochloric acid or sodium hydroxide; reacting for 2-5 hours while keeping the pH value unchanged; reacting overnight at room temperature; finally, performing dialysis and purification on the obtained reaction solution for 3-5 days in an acid deionized water environment; collecting the product; performing vacuum freeze drying for 48-96h, wherein the obtained white flocculent grafted product is a dopamine-hyaluronic acid composite material. The coating thickness is controlled by changing the molar ratio of the raw materials as well as the product concentration, and the coating material can effectively promote specific adhesion of dental pulp stem cell to the root canal wall.

The present invention belongs to the field of organ regenerating technology, and is especially tooth regenerating technology with keratin stem cell and dental pulp stem cell. The technological scheme of the present invention includes: taking out dental pulp tissue form cheek tooth, digesting with digestive enzyme and transferring to culture liquid; culturing for 24 hr, thrice washing with PBS to eliminate un-adherent cell and transferring to culture liquid; further subsequent culturing the adherent cell for 14-16 days; inducing dental pulp stem cell with growth factor MBP4 and FGF10 for 24 hr, recombining in vitro cultured keratin stem cell and dental pulp stem cell to form recombinant ball; culturing the recombinant ball in culture box at 37 deg.c and with CO2 in 5 %, transplanting into oral cavity and culturing for 10-12 weeks to form tooth. The present invention has expanded material source.

The invention provides a dental pulp stem cell collecting kit. The dental pulp stem cell collecting kit comprises a box body and a box cover connected with the box body via hinge joint; the box body is composed of a first box body and a second box body; the first box body is divided into a plurality of reagent bottle grooves by separating plates; reagent bottles are placed in the reagent bottle grooves; the second box body is divided into a plurality of storage tanks; and a teeth fixing device, a nerve broach, a scalpel, and a cell filter screen are stored in the storage tanks respectively. The dental pulp stem cell collecting kit can be used for processing of detached teeth conveniently; a culture liquid provided by the dental pulp stem cell collecting kit is capable of increasing amplification times of dental pulp stem cells obviously; a provided stem cell preservation liquid is capable of realizing long term preservation of dental pulp stem cells; and after recovery, the preserved stem cells are still high in activity.

As the percentage of senior citizens increases at the present time, the purpose of the present invention is to provide a cosmetic which can maintain the skin in a healthy state by preventing damage to the skin that accompanies aging, more specifically, discoloration and wrinkling, is very safe, does not pose ethical problems, and can be supplied in a sufficient amount. The present invention provides a cosmetic that comprises as the main component a powder of the supernatant produced by culturing the bone marrow or dental pulp stem cells of a nonhuman mammal. Moreover, provided is a method for ion introduction for protein by which the cosmetic is introduced by ion introduction.

The invention discloses a dental pulp stem cell culture medium. IMDM is taken as a basal culture medium, SITE100* and ascorbic acid 200-400 mM are included in 1 L of culture medium, the SITE serves as an indispensable component for cell growth, the ascorbic acid and fibronectin are helpful to a dental pulp stem cell to form cell epimatrix, and are favorable for the growth of the dental pulp stem cell. The dental pulp stem cell culture medium comprises 1 to 10 ug of PDGF, 1 to 10 ug of hydrocortisone, 1 to 5 ng of EGF, 1 to 5 ng of b-FGF1, 50 to 200 ng of PTH, and 1 to 20 mM of dexamethasone, the combination of all growth factors can effectively enhance the multiplication capacity of the dental pulp stem cell, and can keep the undifferentiated state of the cell. The culture medium provided by the invention can quickly expand and increase the dental pulp stem cell and keep the multi-directional differentiation capability of the dental pulp stem cell, reaches an expanding and increasing speed which is increased by 2 to 3 times by compared with that of a conventional culture medium, and has very high scientific research and clinical application values.

The invention relates to the technical field of stem cell culture and in particular relates to a culture medium and application thereof as well as a method for culturing dental pulp stem cells. According to the culture medium, a serum replacement, an EGF (Epidermal Growth Factor), an FGF (Fibroblast Growth Factor), a PDGF (Platelet Derived Growth Factor), a VEGF (Vascular Endothelial Growth Factor), L-glutamine, albumin, insulin and soybean isoflavone are added into a serum-free culture medium; each factor and the soybean isoflavone have a good promotion effect on growth of the dental pulp stem cells. An experiment shows that a cell period can be shortened when the culture medium is used for culturing dental pulp mesenchymal stem cells, the cell proliferation ability is improved, and the purity and dryness of the stem cells are improved; the culture medium does not contain animal derived ingredients including fetal bovine serum and the like, and the stem cells obtained by the culture medium is suitable for clinical application.

The invention relates to a culture medium for human dental pulp stem cells and a preparation method for human dental pulp stem cells and belongs to the technical field of stem cell preparation. The culture medium for human dental pulp stem cells, provided by the invention, is based on an alpha-MEM culture medium and contains 5%-10% of human AB serum by volume percent, 100mu M ascorbic acid, 2mM L-glutamine, 100U / ml penicillin sodium and 100mg / ml streptomycin. The dental pulp stem cells prepared by the culture medium provided by the invention are high in yield, are free from human immunological rejection and can supply reliable seed cells for repairing and regeneration of clinical teeth.

Disclosed is a root canal filler for non-extracted tooth which causes no internal resorption or external resorption in a tooth with complete root formation, shows no odontoclast, and contributes to the regeneration of a dental tissue in which odontoblasts are smoothly aligned on the dentin wall. After pulpectomy or enlargement / cleaning of an infected root canal, a root canal filler for non-extracted tooth, which comprises tooth pulp stem cells and an extracellular matrix, is inserted into the apical side of the root canal of the non-extracted tooth. The tooth pulp stem cells may be, for example, dental pulp CXCR4-positive cells. It is preferred to attach, to the crown side of the root canal, migration factor(s) including at least one factor selected from among a cell migration factor, a cell proliferation factor, a neurotrophic factor and an angiogenic factor.

The invention discloses a bone-induced composite silk fibroin stent for MRI sialography and a preparation method thereof. The volume of the composite silk fibroin stent is 1. 5-2. 0 mm<3>, and the composite silk fibroin stent is loaded with 3*10<5> - 7*10<5> dental pulp stem cells; and the composite silk fibroin stent is prepared by taking a dispersing solution as a raw material, wherein the dispersing solution is formed by 2-10% of silk fibroin solution, hydroxyapatite and ultra paramagnetic ferroferric oxide. According to the bone-induced composite silk fibroin stent for MRI sialography, the composite silk fibroin stent has suitable pore size and porosity, adhesion and proliferation of the dental pulp stem cells are facilitated, the problems of an existing stent material are solved, various substances are compounded, the advantage of the original substance is maintained, the deficiency of the single original material performance is overcome, the preparation process is simple, the required raw materials are easy to obtain, the prepared composite stents are expected to be widely used in the biomaterial field; and as the marker of the tissue engineering stent, the ultra paramagnetic ferroferric oxide is expected to be applied to clinical medicine.

The invention discloses a dental pulp stem cell exosome preparation as well as a preparation method and application thereof, and relates to the technical field of biology. Dental pulp stem cells can express neuronal cell surface markers, can be differentiated to nerve cells and promote the proliferation of the nerve cells, so that the dental pulp stem cells can be used for preparing cell preparations for repairing nerve injuries such as traumatic brain injuries (TBI). The dental pulp stem cells are used for replacing traditional embryonic stem cells, neural stem cells or bone marrow stem cellsto be used for treating the nerve injuries, and have the advantages of being rich in source, simple and convenient to obtain, low in immunogenicity and capable of avoiding ethical problems. Dental pulp stem cell exosomes can accurately target recipient cells and are used for preparing nerve repair preparations for the injuries such as the TBI, and then treatment of the nerve injuries such as theTBI by the exosomes is achieved. The dental pulp stem cell exosomes can also be used for preparing neuroinflammation inhibitors.

The present invention relates to a method for differentiation of mesenchymal stem cells or dental pulp stem cells. More specifically, the invention relates to a method for differentiating stem cells to neural cells by applying mesenchymal stem cells or dental pulp stem cells with a low-frequency electromagnetic field. The differentiation method according to the present invention can induce differentiation even with low-cost mediums rather than induced neural differentiation mediums which are expensive due to addition of growth factors, and the neural cells differentiated according to the present invention may be useful for treatment of neurological brain diseases.

A dental pulp stem cell resuscitation fluid and a dental pulp stem cell resuscitation method are disclosed, the new stem cell resuscitation fluid is constructed by addition of a certain amount of basic fibroblast growth factors into a conventional stem cell culture medium, the new stem cell resuscitation fluid is used in resuscitation process of DPSCs (dental pulp stem cells) for rapid recovery of activity of the DPSCs in a short period of time, stemness and multiple differentiation potential of the original stem cells can be maintained, the resuscitation time of the stem cells can be shortened, the clinical use survival rate of the stem cells can be improved, the proliferation rate exceed that of a conventional medium group, meanwhile the cell stemness and multiple differentiation potential can be maintained, seed cells with better performance and status can be effectively provided for clinical stem cell therapy, and a lot of time, manpower and material cost can be saved.

The invention relates to the technical field of tissue engineering, and in particular relates to a composite stent material. A preparation method of the composite stent material comprises the steps that a chitosan solution is mixed with an I-type collagen solution; pH is kept acidic; a chitosan / I-type collagen compound is obtained, mixed with a cross-linking agent, and subjected to freeze drying; a multi-hole stent is obtained, and soaked in a serum-free medium; the serum-free medium is removed; a dental pulp stem cell suspension liquid is added; standing is performed; a complete medium is added for cell culture; and the composite stent material is obtained. The research of composite stent material shows that a dental pulp stem cell has better osteogenic differentiation capacity than a bone marrow stem cell; and the dental pulp stem cell can be tightly combined with the stent material, grows well in the stent material well, and can be applied to treatment on a bone defect better.

The invention provides a long-term in-vitro culture and neural directional differentiation method of deciduous tooth pulp stem cells, which can obtain high-activity primary deciduous tooth pulp stem cells more simply, conveniently and efficiently, and can shorten an induction period and improve the conversion rate in a neural directional differentiation process.