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Method for preparing primary dental pulp stem cells and method for constructing dental pulp stem cell bank

A technique for culturing dental pulp stem cells and stem cells, which is applied in the field of constructing dental pulp stem cell banks and the preparation of primary dental pulp stem cells, can solve problems such as incomplete digestion, excessive digestion, and low cell volume, and save time, cost, and shorten the time the effect of time

Inactive Publication Date: 2017-10-20
浙江优牙生物科技有限公司 +2
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  • Claims
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AI Technical Summary

Problems solved by technology

At present, for the extraction and culture of dental pulp tissue, the traditional methods are direct culture of tissue blocks and collagenase digestion and culture. Excessive and other shortcomings

Method used

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  • Method for preparing primary dental pulp stem cells and method for constructing dental pulp stem cell bank
  • Method for preparing primary dental pulp stem cells and method for constructing dental pulp stem cell bank
  • Method for preparing primary dental pulp stem cells and method for constructing dental pulp stem cell bank

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Embodiment Construction

[0030] The present invention will be described in detail below in conjunction with examples, which are only preferred implementations of the present invention, and are not limitations of the present invention.

[0031] dental collection

[0032] After the patients made an appointment and registered in advance, the extracted caries-free healthy wisdom teeth (age ≤ 30 years old) were collected in the outpatient room of the designated stomatological hospital. First, rinse the isolated tooth twice with sterile PBS buffer, 1 min each time; secondly, use sterile ophthalmic curved forceps to carefully clean the residual soft tissue on the surface of the isolated tooth, and rinse the cleaned isolated tooth again with sterile PBS buffer. The tooth was wiped twice, each time for 1 min; finally, the isolated tooth was wiped twice with 75% alcohol cotton ball, each time for 1 min, and then placed in a sterile tooth collection tube containing a 4°C tooth storage solution, and stored in an ...

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Abstract

A method for preparing primary cell dental pulp stem construction and method of dental pulp stem cell library, the use of thermostatic shaker at specific temperature and speed conditions, can be in a few minutes after digestion, to extract enough has good biological activity of dental pulp stem cells, which can shorten the primary dental pulp stem cells generation of extraction time, save time and cost, large scale production can be obtained at the same time, plenty of pulp stem cells, can meet the requirements of dental pulp stem cells in stem cell library and later stem cells for clinical treatment and regenerative medicine research needs, and the pulp tissue under conventional semi digested 30min or full digestion 1H control group compared to the amount of DPSCs had no significant difference, but the cell proliferation activity was significantly higher than the control group. Therefore, this method can be carried out quickly in batches in the construction of DPSCs library, which shortens the time of DPSCs primary extraction, saves time and cost, and is suitable for large-scale production.

Description

technical field [0001] The invention specifically relates to the technical field of stem cell culture, in particular to a method for preparing primary dental pulp stem cells and a method for constructing a dental pulp stem cell bank. Background technique [0002] Stem cells are a type of cells with self-renewal, high proliferation and multi-directional differentiation capabilities, which can be induced to differentiate into mature human tissues and organs under specific conditions, and have been widely used in various aspects of regenerative medicine research. The source of stem cells is mainly embryonic stem cells and mesenchymal stem cells, among which embryonic stem cells are limited due to the ethical issues involved in their clinical translation research; the main source of mesenchymal stem cells is bone marrow, that is, bone marrow mesenchymal stem cells, but in the process of clinical translation research There are disadvantages such as invasive damage to the donor. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C40B50/06
CPCC12N5/0668C12N2509/00C40B50/06
Inventor 叶青松罗丽花郑丽娜王晓燕金玲
Owner 浙江优牙生物科技有限公司
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