211results about How to "Increase proliferative activity" patented technology

The invention relates to a preparation method and application of an autologous CAR (chimeric antigen receptor)-T cell. An established CD28-CD137-CD19-CD3 full-length gene is guided into a T-cell of a patient by a CRISPR / Cas9 technology to prepare the CAR-T cell, and the CAR-T cell is subjected to expansion in vitro and then returns in the body of the patient to perform anti-tumor treatment. Compared with the traditional tumor treatment method, the method has the advantages that the method is cell targeted therapy and small in side effect; the gene modified T cell can stably express an antigen binding domain on the surface and identify a target antigen, and does not have MHC limit; and the tumor treatment effect is improved.

The invention relates to the field of cell culture, in particular to cell cryopreservation liquid, application thereof and a cryopreservation method of megakaryocyte progenitor cells. The cell cryopreservation liquid comprises DMSO, fetal calf serum, dextran, trehalose and albumin. By means of the cell cryopreservation liquid, the cytoactive of the megakaryocyte progenitor cells can be well maintained during the cryopreservation period, and damage to cells in the cryopreservation and resuscitation processes is lowered. According to the cell cryopreservation liquid, application thereof and the cryopreservation method of the megakaryocyte progenitor cells, the cryopreservation and temperature reduction processes are softer, the cells are stored in liquid nitrogen after the temperature reduction process is conducted, the influence on the cytoactive is small, and damage to the cells in the cryopreservation process is lowered. It is indicated by experiments that the vigour of the resuscitated cells can reach 94% one month after the megakaryocyte progenitor cells are cryopreservated in the cryopreservation liquid, and multiplication activity is good, and the cell cryopreservation liquid is significantly superior to the prior art.

The invention relates to a dietary fiber food with various functional actions. The compound dietary fiber food is formed by preparing and processing the adopted main materials comprising 2% to 10% of oligopolymerization xylose, 15% to 50% of poly-glucose, 3.3% to 10% of rivier giantarum refined powder, 10% to 50% of soybean dietary fiber and 10% to 50% of microcrystalline cellulose, and auxiliary materials comprising 0.5% to 2% of citric acid, 0.2% to 0.6% of aspartame and 0.5% to 1.8% of powder essence. Various types of plant fiber give full play to synergistic actions and advantage complementation; by using the compound cellulose, the system improves the function and the metabolism of large intestines, promotes the creepage and the water absorption expansion of intestinal tracts, quickens defecation, maintains the probiotic environment of the intestinal tracts, comprehensively regulates the cholesterol level, prohibits the absorption of a human body to cholesterol, bile acid, heavy metal and edible pigment and reinforces the immunity of the human body.

The present disclosure provides methods for genetically modifying lymphocytes and methods for performing adoptive cellular therapy that include transducing T cells and / or NK cells without prior ex vivo stimulation. The methods typically include engineered signaling polypeptides that can include a lymphoproliferative element, and / or a chimeric antigen receptor (CAR), for example a microenvironment restricted CAR. Additional elements of such engineered signaling polypeptides are provided herein, as well as vectors, such as retroviral vectors, packaging cell lines and methods of making the same. Furthermore, recombinant retroviruses and methods of making the same are provided. Numerous controls are provided, including riboswitches that are controlled, for example in vivo, by nucleoside analogues.

The invention provides a preparation method of universal heterologous CAR-T cells and an application. Constructed CD28-CD137-CD19-CD3 full-length gene is introduced into allogeneic T cells of healthy persons with CRISPR / Cas9 technology, in order to produce CAR-T cells with targeting cytotoxicity; after amplification in vitro, the cells are fed back to patients for carrying out antineoplastic treatment, and in the treatment process, a PNAs method is used for removing lymphocyte in order to avoid antigraft reaction of hosts. The chimeric antigen receptors can be prepared in large scale with T lymphocyte of healthy human, and antineoplastic treatment of heterologous cancer patients.

Disclosed are methods of treating subjects having disorders or conditions characterized by an unwanted immune response including administering an effective amount of an early activation molecule agonist, antagonist or depletor, to the subject. Human monoclonal antibodies specific to the early activation molecules, and methods of use, are also disclosed.

The invention discloses a tissue repair material which comprises an SIS (small intestinal submucosa) and an NGF (nerve growth factor). When the NGF is individually used or simply mixed with the SIS in use, the NGF is rapidly inactivated and is unfavorable for wound repair. However, after the composite material is prepared with the NGF and the SIS according to the specific method, the action time of the NGF can be effectively prolonged, the cell proliferation activity of the NGF-SIS composite material is remarkably enhanced as compared with that of the individually used SIS, and the NGF-SIS composite material plays a role in synergy.

A tissue-engineered artificial skin able to regulate the pigment secretion is configured through externally culturing the ceratin cells, fibroblasts and melanocyte of skin, proportionally mixing by tissue engineering method, and forming artificial skin.

The invention discloses a human umbilical cord mesenchymal stem cell serum-free culture medium and a preparation method thereof, and an acquisition method of a human umbilical cord mesenchymal stem cell serum-free culture solution. The culture medium comprises a basic culture medium and a serum-free culture supernatant concentrated solution, and also comprises the following components and concentrations: 5-100 ng / ml of a vitamin composition, 50-200 [mu]g / ml of grape polyphenol, 1-50 ng / ml of a recombinant human epidermal growth factor, 1-50 ng / ml of a recombinant human basic fibroblast growthfactor, 1-10 ng / ml of a recombinant platelet-derived growth factor-C, 1-10 ng / ml of a recombinant human insulin-like growth factor-1, 50-200 [mu]M of beta-mercaptoethanol, 1-100 [mu]g / ml of human serum albumin, 1-100 [mu]g / ml of glutathione, and 1.2-3.7 g / L of sodium bicarbonate. The human umbilical cord mesenchymal stem cell serum-free culture medium is used for replacing a traditional culture medium containing animal serum to culture human umbilical cord mesenchymal stem cells, and unsafe factors such as human immune response caused by stem cell pollution by pathogens or animal-derived components and heterologous protein are avoided.

The invention discloses a total nutrient meal suitable for being eaten by patients suffering from tumor, which comprises the following components by weight percent: 10-20% of puffing powders, 10-20% of bean flour, 15-25% of maltodextrin, 3-6% of lactalbumin, 3-6% of hydrolyzed lactalbumin, 8-15% of whole milk powders, 3-5% of a medium-chain fatty acid, 3-6% of black sesame oil, 0.8-1.3% of deep sea fish oil, 0.5-1% of reishi shell-broken spore powder, 0.2-0.8% of longan polysaccharides, 0.05-0.1% of arginine, 5% of inulin and 3-6% of powdered sugar. The nutrient meal disclosed by the invention contains the longan polysaccharides, the ganoderma lucidum spores powder, the arginine and the like, so that the nutrient meal has a certain effect of inhibiting the proliferation of tumor cells and enhancing the body immunity function; the lactalbumin, the hydrolyzed lactalbumin and milk powder provide high-quality proteins, and EPA and DHA contained in the deep sea fish oil also have an effect of inhibiting the proliferation of tumor cells, therefore, the nutrient meal disclosed by the invention is a total nutrient meal which is comprehensive and balanced in nutrition, and has a certain effect of enhancing the body immunity function of patients suffering from tumor and inhibiting the proliferation of tumor cells.

The invention discloses an N-alkyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4 alpha-methanonaphthalene-7-amine compound as well as a synthetic method and an application thereof. Longifolene serving as a heavy oil main component is relatively rich terpenoid in numerous natural extracts. The synthetic method comprises the following steps: obtaining isolongifolene through longifolene isomerization, obtaining isolongifolenone through allylic oxidation of the isolongifolene, carrying out dehydration condensation on the isolongifolenone and primary amine so as to obtain imine and obtaining the chiral N-alkyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4 alpha-methanonaphthalene-7-amine compound after reduction. The compound has vey good inhibition activity on candida albicans, aspergillus niger, candida tropicalis, escherichia coli, staphylococcus aureus, pseudomonas fluorescens, bacillus subtilis and the like, a part of compound has good cancer cell proliferation inhibition activity on human breast cancer cells MCF-7, human lung adenocarcinoma cells A549, hepatoma cells HepG2 and SMMC-7721, and the N-alkyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4 alpha-methanonaphthalene-7-amine compound is a potential antibacterial and bactericidal and anti-tumor compound.

The invention discloses a preparation method of a surface coating of intravascular stents or cardiac valves with excellent biocompatibility, which includes the steps: A. the preparation of a coating solvent: heparin is dissolved in deionized water, the pH value thereof is adjusted to 7 to 8 and then an endothelial cell growth factor is added to form a heparin / growth factor solution; collagens are dissolved in acetum to form a collagen solution; B. dip-coating: the intravascular stents or the cardiac valves are immersed in the heparin / growth factor solution and a layer of heparin / growth factor is dipped and coated on the surface of the intravascular stents or the cardiac valves, and then the intravascular stents or the cardiac valves are taken out for being cleaned by the deionized water and being dried by nitrogen gas; and the intravascular stents or the cardiac valves are immersed in the collagen solution, coated with a layer of collagen, and taken out for being cleaned by the deionized water and being dried by nitrogen gas; the steps of dip-coating by the use of the heparin / growth factor and the collagen are repeated in sequence for 1 time to 60 times; finally the step of dip-coating by the use of the heparin / growth factor is carried out again to obtain the surface coating with excellent biocompatibility of the intravascular stents or the cardiac valves. The intravascular stents or the cardiac valves prepared have excellent biocompatibility and low preparation cost.

The invention discloses an immune cell cryopreservation liquid, and a preparation method, a cryopreservation method and an application thereof; the immune cell cryopreservation liquid includes the components by weight: 5%-15% of DMSO, 1%-5% of propylene glycol, 2%-8% of trehalose, 3%-8% of methyl cellulose, 0.3%-1.2% of glutathione, 0.06%-0.14% of sodium pyruvate, and 60%-80% of human donor plasma; the cryopreservation effect is quite good, the immune cells can be effectively protected from freezing injury, physiological functions and biological characteristics of the immune cells after resuscitation are maintained, cell biological characteristics are not changed, and the immune cell cryopreservation liquid can be used in clinical treatment; the immune cell cryopreservation liquid is suitable for long-term frozen preservation of human mononuclear cells and other kinds of immune cells.

A method for preparing primary cell dental pulp stem construction and method of dental pulp stem cell library, the use of thermostatic shaker at specific temperature and speed conditions, can be in a few minutes after digestion, to extract enough has good biological activity of dental pulp stem cells, which can shorten the primary dental pulp stem cells generation of extraction time, save time and cost, large scale production can be obtained at the same time, plenty of pulp stem cells, can meet the requirements of dental pulp stem cells in stem cell library and later stem cells for clinical treatment and regenerative medicine research needs, and the pulp tissue under conventional semi digested 30min or full digestion 1H control group compared to the amount of DPSCs had no significant difference, but the cell proliferation activity was significantly higher than the control group. Therefore, this method can be carried out quickly in batches in the construction of DPSCs library, which shortens the time of DPSCs primary extraction, saves time and cost, and is suitable for large-scale production.

The invention relates to a celery fermented solution, a celery fermented beverage and preparation methods of products and belongs to the technical field of foods and biology. The celery fermented solution is obtained by carrying out enzymolysis on celery and carrying out combined fermentation by adopting various beneficial bacteria including lactobacillus acidophilus, lactobacillus plantarum, bacillus subtilis and the like. The celery fermented solution can be used as a raw material to prepare an enzyme beverage, such as the celery fermented beverage. The celery enzymatic hydrolysatefermented solution can be prepared by a method comprising the following steps: mixing 7 to 8 parts of the celery and 2 to 3 parts of water in parts by mass; crushing a mixture until the average grain diameter is 1mm to 3mm to obtain celery pulp; adding 0.05 percent to 0.2 percent of 10000U / g cellulase and 0.05 percent to 0.2 percent of 20000U / g to 50000U / g pectinase according to the mass of the celery pulp; carrying out the enzymolysis for 1h to 5h under the condition that the temperature is 40 DEG C to 50 DEG C, so as to obtain the celery enzymatic hydrolysatefermented solution. The product of the invention contains a large quantity of water-soluble dietary fiber components and functional oligosaccharide and has a bidirectional regulation effect of improving constipation and reducing diarrhea.

The invention discloses an anti-aging cosmetic composition. The composition comprises the following components in parts by weight: 1-15 parts of plant extract, 2.55-7 parts of emulsifier, 4-26 parts of emollient, 2.5-20 parts of humectant, 0.1-0.3 part of dipotassium glycyrrhizinate, 0.1-2 parts of silica, 0.03-0.07 part of potassium hydroxide, 0.1-0.2 part of thickening agent, 0.05-2 parts of antioxidant, 0.201-1.01 parts of preservative and 40-75 parts of water, wherein the plant extract is mixed liquid of a liquorice extract and an American ginseng extract. According to the anti-aging cosmetic composition, the mixed liquid of liquorice extract and American ginseng extract is applied to the anti-aging cosmetic, the proliferation activity of skin cells can be effectively improved, and the symptoms of skin aging can be reduced.

The present invention relates to follicular stem cell originated from human hair and its amplifying preparation process, and belongs to the field of medical preparation and human cell culture technology. The present invention adopts improved two-step protease digesting method to amplify follicular stem cell originated from human hair and obtains follicular stem cell with active expression of beta-integrin and keratin 19 in an optimized follicular stem cell preparing environment. The optimized micro environment endows follicular stem cell with fast amplification, antisenile characteristic, the epidermic cell-like, fibroblst-like and hair bulb tissue-like structure or functional expression. The present invention makes it possible to provide sufficient seed cell sources for the fast repair and functional reconstruction of skin defect.

The invention discloses a cosmetic containing an earthworm active protein. The cosmetic containing the earthworm active protein comprises main materials and auxiliary materials. The main materials are composed of glycerinum, hyaluronic acid, aloe vera gel, trehalose, water soluble vitamin E, germall plus liquid, the earthworm active protein, a ginseng extracting solution and purified water. The auxiliary materials are one or more of essences, emulsifiers, water soluble jojoba oil, grapes seed oil, carbomer and triethanolamine allantoin. The cosmetic containing the earthworm active protein can be made into toning lotions, wrinkle removing essences, emulsions, face cream and masks according to difference of the selected auxiliary materials. The cosmetic containing the earthworm active protein has the functions of softening and smoothening the skin, moisturizing the skin and delaying senility, and can be used as a skin care product to be directly applied to the skin.

The invention provides a fermentation product compounded plant extract anti-aging repair composition, application thereof and an essence emulsion containing the composition. The anti-aging repair composition comprises a fermentation product extract and a plant extract, wherein the fermentation product extract comprises 20-120 parts of a yeast / grape fermentation product extract, 45-180 parts of a split yeast fermentation product filtrate / lysate / extract and 30-160 parts of an alteromonas fermentation product extract; the plant extract comprises 2-20 parts of a perilla leaf extract, 3-16 parts ofa lantana camara leaf extract, 2-14 parts of bidens pilosa extract, 5-40 parts of an aesculus hippocastanum extract, 5-20 parts of a thyme extract, 3-36 parts of an aloe vera extract, 3-34 parts of acentella asiatica extract and 2-12 parts of a silybum marianum extract. The yeast extract composition disclosed by the invention is compounded with the plant extract composition with high oxidation resistance, so that rich active nutrition is provided for the skin, the injury is repaired, the premature senility is twisted, and the composition has an extremely high wound healing effect.

The invention discloses a relaxing and sleeping health-care food. The food is prepared by mixing the following components in parts by weight: 12.5-25 parts of gamma-aminobutyric acid serving as a relaxing and sleeping component, 6-10 parts of vitamin C and taurine serving as components for enhancing human immunity in the weight part ratio of 2:1, 20-35 parts of malt extract serving as a componentfor promoting digestion and absorption, 20-30 parts of dietary fiber and xylo-oligosaccharides serving as components for regulating intestinal tracts in the weight part ratio of 3:1, 1-5 parts of nano calcium and 14.5-24 parts of maltodextrin. The food can relieve physical and mental stresses, relax mood, assist in sleeping, enhance sleeping quality, improve depression and enhance memory.

This invention is to provide a means capable of obtaining excellent cell proliferation activity without depending on a thickness of a coating layer in a technique of coating a cell culture substrate (cell culture vessel) using a polymer. Provided is a cell culture substrate comprising a coating layer on at least one surface of a polymer substrate, wherein the coating layer includes a copolymer comprising more than 40% by mole and less than 100% by mole of a structural unit (1) derived from carboxyalkyl (meth)acrylate represented by Formula (1) and more than 0% by mole and less than 60% by mole of a structural unit (2) derived from ethylenically unsaturated monomer having a hydroxyl group (the total of the structural unit (1) and the structural unit (2) is 100% by mole).

The invention relates to a preparation method of FGF1 sericin protein gel with activity for promoting cell proliferation and a product thereof. The preparation method comprises the following steps that sericin protein containing human FGF1 protein is extracted by human FGF1 gene modifying silk, and a sericin protein solution containing human FGF1 protein is obtained; after renaturation, an FGF1 sericin protein aqueous solution is obtained after dialysis with water, and FGF1 sericin protein hydrogel is obtained by centrifugation; and the FGF1 sericin protein hydrogel is placed at 4 DEG C, an FGF1 sericin protein gel material is formed by cross-linking of a chemical cross-linking agent or inducement of an inducer. The prepared gel material has the activity of promoting cell proliferation andhas broad application potential in tissue engineering.

The invention relates to the field of a preparation method and application of a traditional Chinese medicine effective component and in particular relates to a preparation method of rhizoma polygonatipolysaccharide and application of the rhizoma polygonati polysaccharide to the preparation of an immunological enhancement medicine. The rhizoma polygonati polysaccharide is obtained by processing raw rhizoma polygonati, extracting and separating; when an extraction method provided by the invention is adopted, the extraction rate of the rhizoma polygonati polysaccharide is high and the content oftotal polysaccharide is high; the proliferation activity of the rhizoma polygonati polysaccharide on normal mouse spleen cells is improved; cyclophosphamide induced spleen and thymus gland index of mice with low immunity also can be improved; the in-vitro proliferation capability of spleen and thymus gland cells under the stimulation of concanavalin A is enhanced; the phagocytic function of macrophage of the mice with the low immunity and the capability of secreting IL-6 and TNF-alpha are enhanced; the rhizoma polygonati polysaccharide has actual application value in the aspect of treatment of diseases including immunodeficiency diseases, tumors and the like.

The invention discloses a preparation method for autologous-serum antigen-sensitized DC-CIK cells. The preparation method for the autologous-serum antigen-sensitized DC-CIK cells comprises the steps: A) preparation of an autologous-serum antigen; B) preparation of peripheral blood mononuclear cells; C) separation and culture of DC; D) induction amplification of CIK cells; and E) preparation of the DC-CIK cells through co-culture of DC cells and CIK cells. Compared with the DC-CIK cells prepared by employing a routine culture method, the DC-CIK cells cultured by employing the method are obviously increased in CIK proliferation activity and tumoricidal activity; the average proliferation multiple is 200-500 times and is 2-3 times of that of the DC-CIK cells cultured by employing the routine culture method; and the tumoricidal activity is 70-80% when an in-vitro experiment is performed, and is obviously higher than that of the DC-CIK cells cultured by employing the routine culture method. The method is simple in operation, easily controllable in conditions and relatively low in equipment requirements, and the cell proliferation efficiency of the obtained DC-CIK cells is relatively high.

The invention discloses an oral donkey-hide gelatin preparation and a preparation method thereof, and belongs to the technical field of production of traditional Chinese medicines. The oral donkey-hide gelatin preparation comprises ultrafine donkey-hide gelatin powder, rock sugar, soybean oil, tea oil, yellow rice wine, Jerusalem artichoke, fish bone meal, Radix Astragali, wolfberry fruits, chitosan and a slow release assistant. The preparation method comprises the following steps: preparing a dissolved gelatin solution, blending the dissolved gelatin solution, extracting effective traditionalChinese medicinal health components from the Jerusalem artichoke, Radix Astragali and wolfberry fruits, adding the chitosan to ensure the clearness of the preparation, adding the fish bone meal to supplement calcium element, and adding the slow release assistant to improve the absorption rate of nutrients in the oral donkey-hide gelatin preparation by using the drug effect slow release effect ofthe slow release assistant. The oral donkey-hide gelatin preparation prepared in the invention has an excellent drug absorption efficiency.

The invention relates to the technical field of medicine chemistry, in particular to a heteroaromatic ring compound with a thiosemicarbazone structure unit and a preparation method and application ofthe compound. The heteroaromatic ring compound with the thiosemicarbazone structure unit is chelated with metal ions in gastric cancer cells to form a stable complex, and accordingly the proliferationactivity of the gastric cancer cells MGC803 is inhibited. It is proved through a result of an embodiment that compared with a compound 3-AP, the heteroaromatic ring compound with the thiosemicarbazone structure unit has good proliferation inhibition activity on the gastric cancer cells MGC803.

The invention provides a method for induced differentiation of adipose tissue-derived stromal cells into liver-like cells. The method comprises the following steps: washing and cleaning adipose tissue, and adding isovolumetric tissue digestive enzyme for digestion; after completion of digestion, adding a trypsin solution for digestion; after completion of digestion, carrying out centrifuging so asto remove upper-layer grease and a digestive fluid, wherein precipitates are cells of the stromal vascular fraction of the adipose tissue; resuspending the cells of the stromal vascular fraction withphysiological saline, carrying out sieving so as to remove large tissues, carrying out washing, carrying out centrifuging so as to remove a supernatant, and after washing is completed, subjecting stromal vascular cells to inoculated culture so as to obtain the adipose tissue-derived stromal cells; selecting P3-generation adipose tissue-derived stromal cells for digestion so as to obtain a single-cell suspension, and carrying out inoculated culture with a culture medium overnight; removing a serum-free proliferation culture medium, and adding a first induction culture solution for induced culture; and replacing the first induction culture solution with a second induction culture solution for induction so as to obtain differentiated liver-like cells. The method provided by the invention canobtain functional liver cells by induction.

The invention provides gel jelly for improving joint health, and relates to the technical field of food processing. The gel jelly is composed of the following raw materials in parts by weight: 60-80 parts of purified water, 0.1-20 parts of enzymatic bone powder, 1-8 parts of collagen powder, 0.1-6 parts of chitosan oligosaccharide, 0.1-3 parts of gel powder, 2-10 parts of inulin, 0-3 parts of fructo-oligosaccharide, 1-10 parts of xylooligosaccharide and 0-5 parts of fructose. The invention has the beneficial effects that the gel jelly is prepared from the enzymatic bone powder, the collagen powder, the chitosan oligosaccharide and prebiotics as main raw materials, and the prepared gel jelly product is smooth in mouth feel and high in elasticity, has the function of improving joint health,also has the effects of regulating intestines, removing heavy metals and enhancing immunity, is low in calories and easy to eat, and is novel health-care gel jelly.

The invention provides a tripterine derivative as well as a preparation method and application thereof. The tripterine derivative is a compound of formula I as shown in the description, or a medicallyacceptable salt thereof. In-vitro cell level activity evaporation tests show that the compound of formula I, or the medically acceptable salt thereof, has high anti-tumor cell proliferation activity,and has a good inhibition function on tumor stem cells. Therefore, the invention further relates to application of the compound of formula I in preparing medicines for treating and / or preventing cancer.