131 results about "Hair follicle stem" patented technology

This invention relates to a method and composition for enhancing epidermal and/or hair follicle stem cell production, cell renewal, and/or growth of the skin, hair and nails, by topical administration of a therapeutic dosage of cyanobacteria and green algae and/or both simultaneous enteral and topical administration of a therapeutic dosage of cyanobacteria and green algae.

The present invention is directed to methods for readily propagating somatic hair follicle stem cells or melanocyte stem cells. The methods comprise enhancing guanine nucleotide (GNP) biosynthesis, thereby expanding guanine nucleotide pools. This in turn conditionally suppresses asymmetric cell kinetics in the explanted cells. The methods of the invention include pharmacological methods and genetic methods. For example, the resulting cultured somatic hair follicle stem cells can be used for a variety of applications including cell replacement therapies such as hair transplants, gene therapies, and tissue engineering applications, such as the generation of artificial skin and skin regeneration strategies including skin grafts.

The invention provides a hair follicle reconstruction method based on hair follicle stem cells and fibroblast. The method provided by the invention comprises the steps of: (1) culturing and amplifying the hair follicle stem cells; (2) culturing and amplifying the fibroblast; and (3) mixing the amplified hair follicle stem cells and the amplified fibroblast, and inoculating a mixture to a position to be subjected to hair follicle reconstruction. Because the hair follicle stem cells can be passed massively in a long term and the dermis fibroblast is convenient to obtain, the method provided by the invention has very strong actual application prospect and scientific and research potential, is an important method for reconstructing skin and hair follicles in treating large-area skin injury (such burns, scalds and the like), and is also a first choice to testify the hair follicle stem cells in scientific researches.

The present invention relates to mutant hair follicle stem cells possessing an inactive Bmpr1a receptor, wherein such mutant hair follicle stem cells proliferate and undergo self-renewal. Additionally, the present invention relates to compositions and methods for induction of hair follicle stem cell proliferation in vivo and in vitro. The present invention also relates to mutant Bmpr1a organisms and tumor cells.

The invention relates to a skin renewal method. According to the skin renewal method, hair follicle stem cells are used as seed cells and silica gel dressing is used as a new cell transplantation carrier. The skin renewal method comprises the following steps: 1) cultivating and amplifying hair follicle stem cells; 2) cultivating dermal cells; 3) mixing the hair follicle stem cells cultivated and amplified in the step 1) and the dermal cells cultivated in the step 2); 4) dropwise adding mixed cells obtained in the step 3) into silica gel dressing for incubation; and 5) transplanting the cells and the silica gel dressing used in the step 4) to a part required to be subjected to skin renewal. The method provided by the invention not only can renew functional skin in the treatment of large-area skin injuries, but also can provide a simple validation method for the multipotency test on hair follicle stem cells in scientific research activities.

The invention discloses a hair-growing and/or white-hair-to-black-hair essence liquid containing various peptides and plant extracts. The hair-growing and/or white-hair-to-black-hair essence liquid isprepared from tripeptide-1, copper tripeptide-1, acetyl tetrapeptide-3, a cacumen platycladi extract, a sargassum pallidum extract and bisabolol. According to the hair-growing and/or white-hair-to-black-hair essence liquid, by compounding plant traditional Chinese medicine components and small-molecular peptide components, scalp blood circulation is improved, oxidation resistance and free radicalresistance are achieved, hair follicle tissues are repaired, keratin genes are activated, hair growth is promoted, and a scalp nutrient and healthy environment is maintained. The effects of activating blood, resisting inflammation, inhibiting bacteria, inhibiting sebaceous gland excessive secretion and promoting hair follicle stem cell proliferation and differentiation are effectively achieved, the effects of relieving itching, controlling oil, strengthening hair and preventing alopecia are achieved, white hair becomes black from roots, white hair can be rapidly turned into black hair, and moreover, symptoms of alopecia and dry and withered hair can be effectively eliminated. The components are natural, safe, mild, free of irritation, capable of taking effect rapidly and free of any potential hazard.

The present invention relates to a method of proliferating follicular stem cells in high yield, and more particularly, to a method of proliferating follicular stem cells in large amounts by culturing the cells using a specific medium containing a specific concentration of a Rho-associated kinase (ROCK) inhibitor and to a medium which is used in the method.

Relating to the fields of biotechnologies and cosmetic science, the invention provides a technology for using the umbilical cords and placentas of volunteers to prepare a skin care product for skin rejuvenation. Specifically, the umbilical cords and placentas of term delivery volunteers are separated, blood vessels and envelopes therein are removed, a tissue homogenizing machine is employed to make the obtained tissue into homogenate, and then medical grade glycerin, preservative and the like are added to prepare the skin care product. Being rich in trophic factors, the skin care product has the efficacy of promoting the proliferation of epidermal stem cells and hair follicle stem cells, plays an important role in maintaining skin elasticity and health, and has the advantages of no toxic, side effect or adverse reactions, etc. The umbilical placental skin care product provides a good method for delaying aging and restoring skin elasticity.

The present invention is directed to an enriched preparation of multipotent mesenchymal cells obtained from human hair follicle stem cells, preferably adult human hair follicle cells. These cells can be differentiated into adipogenic, chondrogenic and osteogenic cells as well as smooth muscle cells. The present invention is also directed to a method of producing a tissue-engineered vascular vessel containing the preparation of isolated smooth muscle cells or progenitors thereof. The resulting tissue-engineered vascular vessel and a method of producing a tissue-engineered vascular vessel for a particular patient are also disclosed.

The invention discloses a basic fibroblast growth factor composition, which comprises the following components by mass percentage: 0.01-0.05% of a basic fibroblast growth factor, 1.2-3% of mannitol, 0.05-0.2% of hyaluronic acid, 0.01-0.2% of tea polyphenol and the balance of first purifying water. The invention also provides a preparation method of the basic fibroblast growth factor composition. The invention also provides a hair regenerator, and two preparation methods of the hair regenerator. The invention also provides an application method of the hair regenerator. The basic fibroblast growth factor in the composition can stimulate and promote the differentiation of hair follicle stem cells, prevents the hair follicle cell atrophia-degeneration, has restoration function of the hair follicle cells with atrophia-degeneration, gradually improves the microenvironment of hair root growth, and promotes the full nutrition supply for the root of hair and normal growth of the hair root.

The invention discloses an inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells, comprising: (1), isolating rat hair follicle stem cell; (2), culturing the rat hair follicle stem cells; (3), purifying the rat hair follicle stem cells; (4), inhibiting the induced differentiation of the rat hair follicle stem cells into vascular endothelial cells. In the inhibition method provided herein, vascular endothelial cell growth factor 165 is used for the first time as an inducing factor so that the rat hair follicle stem cells efficiently and directionally differentiate into vascular endothelial cells; the formation and growth of vascular endothelial cells generated by induced differentiation of the hair follicle stem cells can be adjusted, an effective healing of a wound is promoted; it is also possible to provide seed cell sources for solving the problems in vascularization of tissue engineering skin, cell-transplant therapy of ischemic disease and the like.

The present invention relates to a method for isolating hair follicle stem cells and a composition for inducing hair growth. More specifically, relates to a method for isolating hair follicle stem cells showing a positive immunological response to CD34, by chemically degrading hair follicle-containing scalp tissue and then culturing the degraded tissue in a serum-containing medium and a serum-free medium, as well as a composition for inducing hair growth, which contains, as an active ingredient, CD34-positive hair follicle stem cells isolated by the method. The hair follicle-derived stem cells, which are obtained according to the disclosed method, are classified as autologous adult stem cells, have self-renewal capability, the ability to differentiate into adult hair follicle cells and the ability to induce hair growth, and can be used as a novel cell therapeutic agent against hair loss. In addition, the present invention relates to a method for culturing hair follicle cells, which has high yield compared to that of the prior art, as well as a method for identifying hair follicle stem cells.

The invention discloses a preparation method of hair follicle stem cells. The method comprises the following steps: cutting roots of hair follicles of volunteers, performing cleaning, adding 0.25% Dispase enzyme, and performing digesting at 37 DEG C for 0.5-1.5 hours; after enzyme digestion, and inoculating the obtained material into a hair follicle stem cell culture medium, wherein the culture conditions are that the temperature is 37 DEG C, the CO2 content is 5 percent, and a liquid is changed once every 1 to 2 days; subjecting hair follicle stem cells to amplification: transferring the hairfollicle stem cells obtained in the step S2 into a hair follicle stem cell amplification culture medium for continuous culture, wherein the hair follicle stem cell amplification culture medium comprises the following components: a DMEM basal culture medium, FBS, T-beta, hEGF, VEGF and Rho related kinase; and subjecting the hair follicle stem cells to purification. The hair follicle stem cells prepared by the method disclosed by the invention can be subjected to long-term in-vitro subculture, have very high regeneration and differentiation capacities, lay a foundation for being applied to treatment of skin defects, and have a very good clinical application prospect.

The invention relates to the field of stem cell induction culture, in particular to a culture method for induced differentiation of hair follicle stem cells into vascular endothelial cells. The culture method for induced differentiation of the hair follicle stem cells into the vascular endothelial cells comprises steps as follows: (1) separation of rat hair follicle stem cells; (2) culture of the rat hair follicle stem cells; (3) purification of the rat hair follicle stem cells; (4) culture of in-vitro induced differentiation of the rat hair follicle stem cells into the vascular endothelial cells. The vascular endothelial cells for promoting early vascularization of skin after trauma are obtained with the culture method, so that repair of traumatic skin is promoted; meanwhile, seed cell sources can be provided for solving problems of vascularization of tissue engineering skin, ischemic disease treatment through cell transplantation and the like.

This invention provides a method for accelerating the proliferation of human mesenchymal stem cells, which comprises the steps of: transfecting recombinant expression carrier containing EGF to human mesenchymal stem cells, and stimulating with a certain concentration of calcium ions during the transfection process to divide into typical stem cells. Under nude mouse subcutaneous microenvironment induction, the stem cells are further divided into skin tissue stem cells, thus directly taking part in the proliferation of nude mouse hair. This invention can obtain rapidly proliferated human mesenchymal stem cells, thus having a potential application in clinical skin wound treatment.

The invention provides a hair restorer. The hair restorer comprises an adipose tissue-derived stromal cell conditioned medium, a fibroblast exosome, mannitol, trehalose and dextran 40, wherein adiposetissue-derived stromal cells are irradiated according to the UVB irradiation dosage of 20-100 mJ/cm<2>, the irradiated adipose tissue-derived stromal cells secrete various types of bioactive factors,hair papilla can be stimulated, hair follicle stem cells can be activated, and the function of hair restoration from inside to outside is realized. The invention further provides a hair restoration composition with a nutritional agent and the hair restorer. The hair restoration composition has a remarkable treatment effect on androgenic alopecia, alopecia seborrhoeica and alopecia areata and is easy to use and popularize.

The invention discloses culturing, screening and amplifying techniques for hair follicle stem cells for clinic treatment level cell therapy. The bottlenecks are always research hotspots and difficulties in the biological stem cell field; currently, by carrying out separation and purification by virtue of remarkable adhering properties of a basement membrane, the hair follicle stem cells are obtained by virtue of three-dimensional culture and amplification measures. By depending on amplification anchorage-dependent cells, a three-dimensional high-simulation in-vivo extracellular matrix system is provided for screening and separating the levels of target cells. The method is a novel culture technique for culture in vitro of biological cells of stem cells of adults (embryo skin) in a high-simulation in-vivo adhesion environment. Skin seed hair follicle cells of targeted tissue engineering cultured through adhesion screening in the environment of a high-simulation substrate have high abdication capacity and multiplication capacity and can form a differentiated cortex structure in vitro. By screening immunogenic cells, immune escape is safely achieved, the transplant time is prolonged, the clinic application of tissue engineering is increased, the healing of wounds is promoted, and a solution with absolute advantages for the clinic treatment level cell therapy is provided for diseases of skin.

The invention discloses a construction method for a human hair follicle stem cell bank. The method comprises the steps of collecting the hair of a donor, acquiring and separating human hair follicle stem cells, cultivating the human hair follicle stem cells, detecting and cryopreserving the human hair follicle stem cells, constructing the bank and the like. According to the method, the human hair follicle stem cells can be effectively separated, purified and amplified, the current situation that such resources cannot be fully utilized is changed, and the stem cell bank for storing the human hair follicle stem cells is established to provide a large number of stem cell resources for clinical and scientific research application; the stem cell bank is constructed according to the acquired human hair follicle stem cells, and is used for storing hair follicle stem cells of an healthy adult and providing clinically applicable human hair follicle stem cells when the healthy adult requires stem cell treatment when being ill, and the stored human hair follicle stem cells can be used for producing a stem cell preparation with permission.

The invention relates to a composition for promoting hair growth. The composition includes lyophilized platelet-rich plasma, and further includes one or more of autologous serum, immune cells and vascular matrix components, and further includes one or more of a hair growth nutrient solution and normal saline. The composition is a non-surgical safe and effective hair growth composition, and is usedfor long term without obvious side effects, from the perspective of cytology and biotechnology, growth factors, nutritional components, immune cells and the like are provided, multi-angle, multi-level and multi-dimension improvement of scalp microenvironment can be improved, scalp local microcirculation is promoted, hair follicle stem cells and hair matrix cells are activated, the hair abnormal resting period is shortened, the secretion of sebaceous glands is effectively inhibited, the physiological function of hair follicles is improved, and quick-acting, intermediate-acting, and long-actingpromotion of hair continuous regeneration is achieved.

The invention relates to a technical method of efficiently culturing down producing goat hair follicle stem cell in vitro. Firstly, separating down producing goat hair follicle: cutting off back and neck skin of the down producing goat; digesting the back and neck skin of the down producing goat by collagenase IV; separating a hair follicle structure and removing of impurities; secondly, after the hair follicle is washed by culture solution, digesting the hair follicle as single-cells in digestive juice, screening the hair follicle by a mesh with 400 meshes and then transferring the hair follicle in hair follicle stem cell culture solution; regarding the hair follicle stem cell cultured on the day that the hair follicle stem cell transferred into hair follicle stem cell culture solution as zero generation; carrying out cell passage once every 3 to 4 days; thirdly, collecting hair follicle stem cell cloning ball of primary culture culturing 3 to 4 days; blowing and beating the primary cloning ball until to one second or one fourth of the primary cloning ball; moving the cell suspension to new culture solution; according to cell density, carrying out cell passage in ratio of 1 to 2 or 1 to 3. According to the method, a lot of hair follicle stem cells can be obtained from limited material sources, the operation is easy, the repeatability is good, and the cultured hair follicle stem cell can be showed as Beta-intigren, oct-4 and is alkaline phosphatas positive, which shows that the hair follicle stem cell has multilineage differentiation potential.