56results about How to "Reduce exposure time" patented technology

The present application provides a kind of puncture needle positioning guide device, including four translation modules;Under the action of the former stage translation module, the latter stage translation module can move along corresponding direction;First support rod assembly is assembled on the third translation module, and the first support rod assembly includes first support rod and the first connecting assembly that is detachably connected with the first support rod, when being in the connection state, the first connecting assembly can rotate relative to the first support rod;Second support rod assembly is assembled on the fourth translation module, and the second support rod assembly includes second support rod and the second connecting assembly that is detachably connected with the second support rod, when being in the connection state, the second connecting assembly can rotate relative to the second support rod;Positioning guide assembly is detachably assembled at the first and second connecting assemblies, for positioning and guiding puncture needle, and the positioning guide assembly includes steel ball used in cooperation with navigation system.The above-mentioned device can be used in cooperation with navigation system to realize accurate positioning and guiding of puncture needle.

The utility model relates to the technical field of oil well liquid drainage, in particular to a negative pressure liquid drainage device. Comprising an upper connector, a sealing conical surface is formed in the upper connector, the lower end of the upper connector is fixedly and hermetically connected with an outer cylinder, the lower end of the outer cylinder is fixedly and hermetically connected with a lower connector, a piston blind plate is arranged in the lower connector, connected with the lower connector through a shear pin and in sealed contact with the upper connector, and a screen pipe is fixedly and hermetically connected in the lower end of the piston blind plate. The lower end of the screen pipe extends to the position below the lower connector and is blocked. The screen pipe and the piston blind plate are automatically released through fracture of the shear pin, external power or manual intervention is not needed, instant communication of a shaft and an oil pipe column is achieved, and the liquid drainage starting time is remarkably shortened.

The invention discloses a one-time laying system for a wave glider and a collaborative operation method of the one-time laying system, and belongs to the technical field of ocean engineering. The system comprises a wave glider and a transferring and laying device. The moving, transporting and laying device is a detachable separation support, and an upper laying support and a lower moving support are connected through a positioning pin. The laying support is provided with a bearing face matched with the ship body, four hanging rings, a supporting rod for supporting the traction machine and a floating body arranged on the middle rear portion. The method comprises the steps that the device is moved to a deck to be locked; the positioning pins are taken down, and the whole support and the glider are vertically hoisted and laid through the four slings; when the glider is immersed in water, the bracket inclines forward by using the floating body, and the glider is automatically separated; and the mother ship leaves and recycles the laying bracket. Rapid, stable and safe one-time laying of the wave glider under the complex sea condition is achieved, the risk of graded laying and umbilical cable winding are avoided, and the operation efficiency and safety are remarkably improved.

The invention discloses a safe laser mosquito killing method adopting multi-echo laser recognition, which comprises the following steps that: a laser radar performs two-dimensional rapid scanning on a specified plane area, and meanwhile, a receiving module receives laser return light reflected back from an object in real time; laser return light is obtained and analyzed in real time, and real-time data are obtained; if a new target is found, the laser radar scans firstly and then scans again, and after it is confirmed that the target is a mosquito, mosquito attack is started; meanwhile, the laser radar continuously emits detection light spots covering a high-power laser light spot area, real-time data are continuously obtained, and if the identification monitoring module finds abnormity, the high-power laser is stopped immediately. According to the application, comprehensive analysis and pre-judgment can be carried out on a complex background environment, the working state is reasonably adjusted to avoid potential safety hazards under the complex background condition, meanwhile, the irradiation time of the high-power laser is accurately controlled, the irradiation time can be greatly shortened when danger occurs, the response time is smaller than or equal to 0.03 millisecond, and the application range is wide. The safety of human eyes can be fully ensured; and compared with a conventional identification mode, the reliability is higher, the response speed is higher, and the safety performance is higher.

The substrate cleaning device and the overturning device thereof comprises a groove, a lifting mechanism and an overturning mechanism. The lifting mechanism is arranged on one side of the groove and is configured to receive the substrate and drive the substrate to lift. The overturning mechanism is arranged on the groove and is configured to overturn the substrate and move between a receiving position and a taking position relative to the lifting mechanism. The receiving position is the position where the overturning mechanism receives the substrate from the lifting mechanism. The taking position is the position where the overturning mechanism takes out the substrate. In the present application, the multiple substrates which are cleaned in the groove can be transferred to the lifting mechanism at one time and then are quickly transferred to the process robot by the overturning mechanism for multiple times. This not only reduces the residence time of the substrates which are cleaned in the groove, but also improves the efficiency of the substrate cleaning device and the transmission speed of the substrate overturning device.

The invention provides a wafer loading, overturning and soaking mechanism, which comprises a base provided with a box body mounting frame and a module supporting structure, and the module supporting structure is provided with a guide rail; the soaking box body is arranged on the box body mounting frame, and the soaking box body is used for containing soaking liquid; and the overturning module is arranged on the module supporting structure, the overturning module comprises an overturning device and a driving assembly, the overturning device is provided with a wafer clamping part, the wafer clamping part is used for bearing a wafer, and the driving assembly is in driving connection with the overturning device and is used for driving the overturning device to overturn and move up and down along the guide rail. According to the invention, the processing precision and the processing efficiency of the wafer can be obviously improved.

The application provides a MEMS microphone, comprising a substrate, a MEMS chip, an ASIC chip and a transfer island arranged on the substrate; the MEMS chip and the ASIC chip are connected through the transfer island; the transfer island is used for forwarding a pressure signal output by the MEMS chip to the ASIC chip; and the ASIC chip is used for converting the pressure signal output by the MEMS chip into a voltage signal. Compared with the prior art, the MEMS microphone of the application connects the MEMS chip and the ASIC chip through the transfer island, thereby avoiding the connection of gold wires, reducing the exposure time of the MEMS chip in a high-risk environment, and reducing the influence of environmental foreign matters on the MEMS microphone.

The invention discloses a preparation method and application of a prostate cancer tissue single-cell suspension. The preparation method of the prostate cancer tissue single-cell suspension comprises the following steps: S1, tissue pretreatment: putting a prostate cancer tissue sample into a low-temperature pretreatment buffer solution, and cutting the prostate cancer tissue sample into tissue fragments; s2, circular enzymolysis: mixing the tissue fragments with a composite enzymatic hydrolysate, and performing digestion for multiple rounds under an oscillation condition; after each round of digestion, collecting supernate into a stop solution containing serum, repeatedly digesting the remaining tissues for multiple times, and combining the supernate to obtain a cell crude suspension; wherein the composite enzymatic hydrolysate contains collagenase, protease, nuclease, an androgen receptor stimulant and / or a ROCK inhibitor; and S3, sequentially carrying out separation and purification on the cell crude suspension through filtration and density gradient centrifugation to obtain the prostate cancer tissue single-cell suspension.

This invention provides an aqueous dewaxing reagent comprising the following components by weight percentage: 0.3-6% surfactant and 0.5-3% composite penetration enhancer, with the balance being water. The composite penetration enhancer is composed of limonene and terpineol, wherein the mass of terpineol is not less than 10% of the total mass of the composite penetration enhancer. The preparation method involves mixing the surfactant, composite penetration enhancer, and water. This invention utilizes a composite penetration enhancer composed of limonene and terpineol, which significantly improves dewaxing speed, reduces temperature dependence, widens the operating temperature window, reduces tissue damage, provides better protection against antigens, and is beneficial for IHC staining.

The invention discloses a preparation method of an Al-RE intermediate alloy based on an alloy opposite doping method, which comprises the following steps: firstly, industrial pure aluminum Al and mother alloy Al-90RE are taken as raw materials, the raw materials are dried according to a mass ratio meeting the requirement of Al-(10-50) RE, then, the raw materials are melted by adopting a two-stage variable-temperature melting method to obtain an Al-RE melt, then, the Al-(10-50) RE melt is refined, and meanwhile, the Al-RE intermediate alloy is prepared by adopting an alloy opposite doping method. The steel ingot mold is preheated, and finally, the refined Al-RE melt is poured into the preheated steel ingot mold to be cooled, so that an Al-RE intermediate alloy ingot based on the alloy opposite doping method is obtained; rE is one of La, Ce or La + Ce alloy. The obtained Al-RE intermediate alloy is compact in metallographic structure and less in inclusion; in addition, the yield is greater than 90.0%.

The utility model belongs to the field of picking tools, and provides a portable morchella sporocarp picking tool which comprises a picking frame, a synchronous transmission piece, a pushing piece and a clamping hook piece, two sets of sliding grooves are symmetrically formed in the two sides of the inner wall of the picking frame, sliding blocks are connected into the sliding grooves in a sliding mode, a movable plate is connected between the two sets of sliding blocks located on the same side, and the movable plate is connected with the synchronous transmission piece. Cutting blades are arranged on the opposite sides of the two groups of moving plates; the cutting blade is arranged on the moving plate and is controlled to move through the pushing part and the synchronous transmission part, when the cutting blade is not used, the blade is relatively located on the inner side of the picking frame, the exposure time of a blade body is shortened, and the risk of accidentally injuring a picker is greatly reduced; the two groups of moving plates can move synchronously to drive the two groups of cutting blades to approach to complete the shearing action, so that the operation is simple and labor-saving, the complexity and the labor intensity of the operation are reduced, and the operation stroke is shortened.

The present application relates to a kind of microneedle transdermal drug delivery technology, in particular, it relates to a kind of drug-loaded table transdermal nursing system.The drug-loaded table transdermal nursing system includes penetration device, penetration device includes penetration unit, and the penetration unit has substrate and the penetration micro-unit formed on substrate;It further includes delivery device;Delivery device includes delivery unit, and the delivery unit has drug-loading platform, and drug-loading surface of drug-loading platform is provided with drug coating, and drug coating has water-soluble and contains active ingredient;Delivery device can be delivered active ingredient by the reciprocating touch of the skin of specific area multiple times, and when the skin of specific area is touched each time, drug coating partially dissolves, and when the number of touch reaches specific number, drug coating completely dissolves.The nursing system is simple and convenient to operate, can realize the accurate, stable control of drug dosage, can be implemented uniform nursing to specific skin area, and can be suspended or discontinued at any time.

The present invention provides a construction method for a locally deepened open-cut shaft, which relates to the technical field of tunnel construction and includes: first constructing a diaphragm wall, excavating to a first preset elevation to construct the first ring beam, then excavating down to a second preset elevation, and constructing the second ring beam and the first side wall between the first ring beam and the second ring beam; taking the bottom of the second ring beam as the excavation surface, constructing the shaft foundation and the locally deepened foundation, arranging a vertical support member between the top surface of the locally deepened foundation and the bottom surface of the second ring beam, and constructing the remaining side walls and ring beams from bottom to top relying on the vertical support member and the first side wall until the top of the shaft is closed, and finally constructing the second side wall between the top surface of the locally deepened foundation and the bottom surface of the second ring beam. This method improves the closing speed and construction safety of the main structure of the locally deepened open-cut shaft. By constructing the ring beam as a permanent support structure, the demolition process of the temporary waist beam is eliminated, and the problem of asynchronous construction of the left and right side walls in the traditional top-down method is solved.

The present application relates to the technical field of medical devices, in particular to a wound debridement device for blood tumor patients, comprising a handle vertically arranged, an injection part, an adsorption part and a mounting part for clamping medical cotton, the lower end of the handle is provided with a placing cavity, the mounting part is arranged in the placing cavity, the injection part comprises a first cylindrical cavity, a first button, a vertical slide and a first flow channel arranged in the handle, the mounting part comprises a fixing block arranged in the placing cavity and detachably connected with the handle, when the medical cotton is clamped on the handle by the fixing block, part of the medical cotton extends out of the lower end of the handle through the center hole of the fixing block, and the adsorption part comprises a second cylindrical cavity, a second button and an adsorption channel arranged vertically. The present application is simple and fast in operation when debriding the wound of the patient, greatly shortens the debridement time and reduces the labor intensity of medical staff, simultaneously reduces the time of exposing the wound of the patient to the outside, reduces the risk of wound infection and improves the wound healing speed.

The invention discloses a mechanical rapid supporting method for a high-crustal-stress rockburst tunnel, and relates to the technical field of tunnel excavation and supporting. The mechanical rapid supporting method comprises the steps that S1, early-stage monitoring and risk zoning are conducted; s2, a first-level flexible layer is constructed; s3, mounting and adjusting a secondary rigid layer; s4, performing real-time monitoring and dynamic regulation and control; and S5, checking the operation quality and circularly summarizing. According to the invention, based on a micro-seismic monitoring system and a fiber grating sensor, initial surrounding rock micro-seismic signals are collected, a rockburst risk area is identified in real time through full fusion of multi-dimensional data, a supporting mechanical arm is linked through a PLC control center, and dynamic adjustment of supporting is carried out and the supporting speed is increased through a multi-stage system supporting system and intelligent monitoring and feedback control; and the trolley integrates laser radar and background positioning, an operation path can be automatically planned according to a high-risk area defined by micro-seismic monitoring in the construction process, automatic obstacle avoidance is achieved, and therefore the damage rate of rockburst is reduced.

The utility model relates to a multi-working-condition spraying type liquid cooling device and mobile equipment, the multi-working-condition spraying type liquid cooling device comprises an evaporation section, a condensation section, a mounting bracket and a spraying adjusting mechanism, the evaporation section is provided with an evaporation cavity, a power element is mounted on the bottom wall of the evaporation cavity through the mounting bracket, and the power element and the evaporation cavity are arranged at an interval; the spraying adjusting mechanism comprises a controller, a liquid pump, a connecting pipeline, a power supply, a liquid level sensing element and nozzles, and the liquid pump is arranged in the evaporation cavity and connected with the nozzles distributed around the power element through the corresponding connecting pipeline. And the controller controls the liquid pump to start, and enables the nozzle at the corresponding position to spray the liquid working medium towards the position, exposed out of the liquid level, of the power element. According to the multi-working-condition spraying type liquid cooling device and the mobile equipment provided by the invention, the problem that a power element is easily subjected to dry burning or overheating due to sudden acceleration change generated by the mobile equipment in operation is solved.

The invention relates to an anti-fogging self-cleaning laparoscope which is characterized in that a transparent glass sheet is fixedly arranged at the outer end of an objective lens at the far end of the laparoscope, and a closed carbon dioxide gas groove is formed in a gap between the transparent glass sheet and the objective lens; an air inlet pipeline is fixed along the inner wall of an outer tube of the laparoscope, a corresponding air inlet hole is formed in the near end of the air inlet pipeline, and the far end of the air inlet pipeline penetrates through the objective lens and extends into the carbon dioxide gas groove; a gas outlet pipeline is fixed along the inner wall of the outer tube of the laparoscope, the far end of the gas outlet pipeline extends into the carbon dioxide gas groove, and the near end of the gas outlet pipeline exhausts gas through a gas exhaust hole; and the air inlet is externally connected with a carbon dioxide air source. The laparoscope solves the technical problems that an existing laparoscope is prone to causing fogging of an objective lens and infection of tissue fluid in a minimally invasive abdominal surgery, consequently, the surgical field is blurred, and surgical operation needs to be interrupted due to the fact that a real-time processing function does not exist.

The invention provides an underground heading machine space section supporting material bidirectional carrying robot dog and a carrying method thereof, and relates to the technical field of coal mine underground intelligent transportation. The robot dog comprises a robot dog main body, a bearing tray, a sensor module and a central controller, the robot dog main body is used for driving the leg module to move through the motor under the control of the central controller so as to autonomously complete a cargo carrying task; sensor modules connected with the central controller are arranged at the front end and the rear end of the robot dog main body, so that 360-degree road condition recognition is realized; the object bearing tray is fixed to the back of the robot dog body and used for bearing and carrying boxes for storing goods. The central controller performs automatic positioning, path finding and obstacle avoidance through visual analysis according to data acquired by the sensor module, and controls the robot dog main body to freely walk in a narrow space section of a coal mine; and the motor is controlled at any time according to needs to adjust the state of the leg module, so that the advancing direction of the robot dog main body is changed without turning around.

The invention relates to the technical field of water conservancy and municipal pipeline construction, in particular to a receiving method of a pipe jacking machine head in a tunnel, which comprises the following steps: S1, fixing a receiving tackle at a first receiving end, and enabling the receiving tackle to be mounted corresponding to a first pipe jacking section; s2, the pipe jacking machine head extends out of the first pipe jacking section and is moved to the receiving tackle, and the pipe jacking machine head is fixed to the receiving tackle; s3, a first trailer is connected to one side, facing the second receiving end, of the receiving tackle, and the receiving tackle is unfixed; s4, dragging the receiving tackle towards the second receiving end by using the first trailer, and enabling the receiving tackle to reach the branch hole; s5, a second trailer is connected to the side, facing the first receiving end, of the receiving pulley; and S6, dragging the receiving pulley into the adit by using a second trailer. The herringbone intersection structure is combined with a two-way dragging method, the receiving and moving efficiency of the pipe jacking machine head is improved, a tunnel enters the follow-up secondary lining construction link as soon as possible after the pipe jacking machine receives and moves, and the collapse risk of a tunnel primary support is reduced.

The invention relates to a campus building structure. The campus building structure comprises a building and a first playground, the building comprises an overhead layer and floors, the overhead layer is arranged on the ground, the floors are arranged above the overhead layer, and hollow spaces are formed in the floors, so that the building is arranged to be an enclosed building. The first playground is arranged on the empty space. The campus building structure has the advantages of being high in space utilization rate and good in applicability.

The present application relates to the field of intelligent water management, and particularly relates to a water abnormal event early warning system based on data collection and analysis, which forms time synchronization time series data through multi-source data collection, calculates dynamic risk scores, identifies abnormal events and outputs event type confidence, generates propagation prediction results in combination with pipe network topology, executes dynamic control measures, and receives feedback to update the state through the alarm closed loop, realizes real-time early warning and accurate disposal of water abnormal events, realizes early identification and accurate attribution of short-time negative pressure backflow and high-toxicity small-volume pollution groups through multi-source time synchronization and dynamic risk assessment, gives the influence range and time window in combination with topological propagation prediction, improves the disposal directionality, and updates the state relying on the alarm-control-feedback closed loop, self-optimizes the threshold and strategy, and improves the emergency efficiency.

The utility model discloses an automatic loading and unloading polyester dyeing integrated device, which relates to the technical field of polyester dyeing and comprises a base, a support plate is fixedly mounted at the top of the base, a transverse connecting block is fixedly mounted at the bottom end of the side wall of the support plate, and a connecting rod is rotatably mounted at the side end of the transverse connecting block. A guide plate is rotatably mounted at the bottom end of the connecting rod, a fixing column is fixedly mounted on the inner wall of the guide plate, a guide rod is rotatably mounted on the outer wall of the fixing column, a vertical connecting block is rotatably mounted at the top of the guide rod, and a cloth suction machine is fixedly mounted at the top of the vertical connecting block and slidably mounted on the inner wall of the supporting plate. According to the utility model, through the synergistic effect of the components such as the cloth suction machine, the guide plate and the inclined filter plate, the automatic feeding, dyeing, discharging and drying processes of polyester materials are realized, an operator is prevented from being in direct contact with a high-temperature dyeing solution, and the exposure time of the operator in a high-temperature environment is shortened, so that the health risk is reduced.

The invention specifically discloses a myofibrillar protein-sunflower seed oil-high M type alginic acid ternary composite probiotic microcapsule and a preparation method thereof, and belongs to the technical field of food. Myofibrillar protein and sodium alginate are used as inner wall materials, gelatin and sunflower seed oil are used as outer wall materials, and lactobacillus acidophilus is embedded in a double-layer mode through electrostatic interaction. The specific preparation method comprises the following steps: mixing a bacterial suspension with the composite wall material, dropwise adding the mixture into pure water, and carrying out ultrasonic curing, dewatering and vacuum freeze drying to obtain the microcapsule. The microcapsule prepared by the invention has a good embedding rate, can play a role in protection and slow release in a simulated digestion process, improves the thermal stability and storage stability of probiotics, and has a wide application prospect.

The invention discloses a shaft combined construction method, and relates to the technical field of shaft construction. Comprising the following steps that firstly, construction preparation is conducted, a raise boring machine is selected, and well mouth positioning and raise boring machine leveling are conducted; step 2, guide hole construction: adopting graded drilling to form a guide hole penetrating through the upper and lower horizontal planes of the vertical shaft; 3, expanding excavation construction is carried out, slope expanding, drilling and blasting operation is carried out, and vertical shaft full-section zoning and layering expanding excavation is carried out from top to bottom with the guide hole as the center; fourthly, primary supporting is carried out, and supporting is synchronously carried out on the expanding excavation face when one layer is expanded and excavated in the zoning and layering expanding excavation process; and 5, secondary supporting is conducted, specifically, after expanding excavation and primary supporting construction are conducted to the bottom of the vertical shaft, secondary supporting is conducted on the wall face of the vertical shaft from bottom to top through the slip form. The mode that the raise boring machine guide hole and manual expanding excavation are combined is adopted, the problems of ventilation, drainage and deslagging existing in the manual expanding excavation process are solved in advance through the guide hole, the technology of secondary lining from bottom to top of the slip form is combined, and the time of the supporting procedure is greatly shortened.

The application relates to the technical field of bridge construction equipment, in particular to a prefabricated and assembled rhombic hanging basket and a mounting method thereof. The rhombic hanging basket comprises a main truss system, a walking system, a bottom basket platform, a suspension system and a composite anchoring system. The mounting based on the rhombic hanging basket comprises the following steps: prefabricating a foldable rhombic truss; using a hoisting device to pull the truss to unfold and pin shaft locking; installing a walking system and other components, using multiple frequency conversion winches to cooperate with displacement sensors, and using a synchronous deviation control algorithm to hoist the bottom basket platform as a whole. In the hanging basket, the rhombic truss can be folded and unfolded, the convenience of transporting and installing the hanging basket on site and putting into construction is improved, the walking system and the composite anchoring system are matched, the efficiency of moving the hanging basket is improved, the automatic synchronous control technology solves the hoisting control problem of the bottom basket under complex environment, the construction efficiency and safety of the hanging basket as a whole are improved, and the risk of high-altitude operation is reduced.

A coil member according to an embodiment includes a substrate including a first surface and a second surface opposite the first surface; a wiring pattern disposed on the substrate; and a metal coating pattern connected to the wiring pattern, the metal coating pattern being thinner than the wiring pattern.

The application discloses a tunnel section delay blasting excavation method with grading, layering and porosity linkage, which comprises the following steps: according to the rock strength, integrity coefficient and underground water state of surrounding rock, a tunnel face is divided into several surrounding rock grading areas; an excavation section is divided into several excavation layers along the vertical direction, and the control surrounding rock grade corresponding to each excavation layer is determined; the representative porosity of the rock mass in each layer is measured, and the blast hole arrangement parameters and single-hole charge quantity of each layer are determined; according to the control surrounding rock grade and representative porosity of each excavation layer, the delay blasting time sequence of the blast holes in different positions in each layer is determined, and each blast hole is blasted according to the delay blasting time sequence; after blasting and ventilation, concrete is sprayed to close the rock surface, and a steel arch is installed. The application realizes fine design of blasting parameters and differential control of the section delay blasting time sequence, thereby effectively inhibiting the large deformation of soft rock, reducing the collapse risk, and improving the construction safety and efficiency.

This invention discloses a method and apparatus for quick-change of electro-hydraulic tool heads for nuclear facility decommissioning robots, relating to the field of nuclear facility decommissioning technology. The apparatus includes a tool head connection assembly, a quick-change adapter assembly, a rotating assembly, and a robot connection assembly. The tool head connection assembly is used to mount the tool head. The quick-change adapter assembly is detachably connected to the tool head connection assembly and connects the tool head to the nuclear facility decommissioning robot's electrical and hydraulic circuits, and is connected to the robot connection assembly via the rotating assembly. The robot connection assembly is used to mount the nuclear facility decommissioning robot. This invention addresses the radioactive environment of nuclear facility decommissioning by providing a fast, safe, and efficient tool head replacement mechanism, reducing the working time of personnel in radioactive areas, lowering the radiation dose received by personnel, and significantly improving the operational performance and safety of nuclear facility decommissioning robots.

This utility model discloses an antibacterial hose cap, including a main support cap. A sealing sleeve is abutted to the bottom of the main support cap, and an isolation plate is fixedly installed on the inner side of the sealing sleeve. A through groove is formed on the bottom surface of the isolation plate, and a movable top groove is formed on the top of the main support cap. The sealing sleeve is fitted onto the outer side of the hose, and the isolation plate further isolates and seals the outer side of the hose, further enhancing the sealing effect. This allows for the separation of the sealing top block from the hose, making operation simple and convenient. No complicated tools or tedious disassembly steps are required; ordinary users can easily replace the sealing top block, saving time and effort. Pressing the top block and the inner top block automatically resets, and the new sealing top block quickly and tightly engages with the top opening of the hose, achieving rapid sealing and reducing the hose's exposure time during sealing top block replacement, thus reducing the risk of leakage and contamination.

This invention discloses a method for constructing a dual-omics library based on single-cell whole-genome methylation and transcriptomics, belonging to the field of sequencing technology. The method includes: lysing single cells in a sorting tube pre-filled with lysis buffer; applying a nucleophilic coating to the inner wall of the sorting tube to allow cell nuclei to adsorb and obtain a supernatant containing mRNA; reverse transcribing the supernatant to obtain cDNA and constructing a transcriptome library; lysing the cell nucleus to release DNA and converting it to bisulfite; ligating the converted DNA with a molecularly tagged adapter to obtain methylation intermediates; treating with exonucleases; merging intermediates with different molecular tags; purifying with magnetic beads, single-stranding, ligating the other end with a sequencing adapter, and amplifying and purifying to obtain a methylated library. This invention achieves simultaneous separation of RNA and DNA in the same tube for library construction, reducing separation and transfer steps, minimizing RNA degradation and nucleic acid loss, and improving the throughput of methylation library construction and the consistency of dual-omics data.