100 results about "Γ ray irradiation" patented technology

The invention discloses a method for amplifying NK cells of human beings under the condition of in vitro culture, which is characterized in that a peripheral blood mononuclear cell (PBMC) is taken asthe original culturing material, and the PBMC is cultured together with an engineering cell which is built by adopting the genetic engineering method and is used for stimulating the growth of the NK cell. The built engineering cell which is used for stimulating the growth of the NK cell expresses several cytokines (IL-2, IL-12, IL-15, IL-18, 4-1BB) which can promote the growth of the NK cell on the surface of the K562 cell; after irradiation and inactivation with gamma-rays, the engineering cell is cultured with the PBMC in vitro, as a result, the amplification effect of the stimulation methodin the invention is hundreds of times stronger than that of the currently universal method in which soluble cytokines are added purely to the culture solution; and after cultured for 3 weeks, the non-NK cells in the PBMC are mainly dead and disappeared, the NK cells are proliferated in great quantity, the purity of the NK cells reaches over 96% and the total number of the NK cells is amplified byover 1500 times.

A process for preparing the dried active amnion includes such steps as choosing health placenta tissue, sterilizing, separating amnion, removing sponge layer, spreading it on nitro-cellular paper, shearing by needed sizes, incubating in trypsase solution and/or EDTA solution, removing epithelial cells, flushing by physiologic saline, vacuum freeze drying, vacuum packing and gamma-ray radiating.

A method is described for producing a radionuclide product B. A target is provided which includes an amount of a nuclide A. A gamma (γ) beam from Compton back-scattering of laser light from an electron beam irradiates the target and thereby transmutes at least a portion of the amount of the nuclide A into the product B. Providing the target includes selecting a nuclide A which is transmutable into product B by a gamma (γ) induced nuclear reaction.

The present invention relates to a method of preventing or treating in a subject a disorder characterized in a reduced GABA receptor function by administering to the subject a therapeutically effective amount of a pharmacologically active blood serum product obtainable by a method comprising electrostimulation of a non-human animal, withdrawal of blood from said animal isolation of serum from said blood, and gamma irradiation of said serum.

A novel method of preparation of easily stripped off temporary wound dressing material is disclosed. In this process, the-N-isopropyl acrylamide (NIPAAm) monomer is successfully grafted on the non-woven cloths by copolymerization. It is initiated by gamma -ray irradiation to activate the surface of the non-woven cloth. NIPAAm is then grafted onto the surface of the non-woven cloth. The free radical or peroxide is produced by Co-60 gamma -ray, then grafted on the non-woven cloths. The lower critical solution temperature (LCST) in thermoresponsive poly-N-isopropylacrylamide (NIPAAm) is still retained after the grafting. This will make the dressing cloth stripped off easily and without hurting the tissue. The material process is very simple and has medically applicable value.

A sea chest cover for providing access to the sea chest of a ship. The sea chest cover includes both a cover element and a liner element received within the interior of the sea chest and is fabricated essentially entirely of one or more viscoelastic materials, preferably either polyethylene or polyurethane. Materials can be also made of copolymers, which may be laminated, and compression molded or portions may be blow molded. Fabrication of the sea chest cover from a viscoelastic material reduces or eliminates corrosion and erosion problems. It also minimizes marine organism and ice build-up. The result is a reduction in the expense of associated with maintaining such covers. The sea chest cover can also include an additive to reduce bacteria and algae growth and be irradiated with gamma rays for increased strength.

The invention discloses a Streptococcus equi subsp. Zooepidemicus subspecies SXY36 and an application in the fermentation production of hyaluronic acid. The SXY36 strains of Streptococcus equi subsp. Zooepidemicus subspecies and a method for controlling HA molecular weight using the fermentation process are provided. The method has the advantages that SXY36 is subjected to irradiation mutation breeding by ultraviolet rays, microwave and gamma rays, no hemolysis exists, the HA fermentation yield is high, and the heredity is stable, in the optimal fermentation condition, the fermentation yield of shaking flask is up to 0.731 g/L, and the yield of fermentation by a fermentation cylinder can reach 4.78 g/L; a two-stage variable temperature fermentation and stable pH method is adopted, high molecular-weight HA can be prepared by fermentation, and the molecular weight can reach 1.89 MDa, which is two times the molecular weight by a conventional fermentation method; HA with the molecular weight in the range of 0.035-1.89 MDa can be produced by using a fermentation broth self-enzymatic degradation method to prepare different molecular-weight HA and by controlling the time of enzymatic hydrolysis.

The invention relates to a sterilization treatment method for a biological coating medical device, wherein a biological activity protective coating is coated outside a biological coating on the surface of the medical device, is subjected to freeze-drying treatment and is sterilized by the radiation of Co60-gamma rays. The germicidal treatment method provided by the invention adopts the biologicalactivity protective coating, and can avoid the radiation of Co60-gamma rays from damaging biological activity compositions while ensuring the sterilization effect; moreover, the method also has the advantages of simple process, safe use and high industrial practicability.

A broad spectrum disinfectant includes a quaternary ammonium halogen, an alkaline agent, a chelating agent, a nonionic surfactant coupler, at least one alkoxylated nonionic surfactant, and water or any aliphatic alcohol. The disinfectant composition is phenol-free, is effective in eradicating microorganisms such as various fungi, and is stable to gamma-irradiation.

The invention discloses composite polysaccharide for resisting radiation. Effective ingredients of the composite polysaccharide comprise lentinan, agaricus blazei polysaccharide and tuckahoe polysaccharide. The composite polysaccharide can obviously improve the immunity of mice, can enhance the white cell number and the bone marrow cell DNA content of the mice after gamma-ray irradiation, has stronger radiation resisting effect, and is particularly suitable for tumor disease radiation therapy and chemotherapy or adjuvant therapy after tumor disease operation.

The preparation process of heterogeneous bone transplanting material includes the following steps: 1. washing animal bone as the material with high pressure pulse distilled water or normal saline at normal pressure and room temperature and in aseptic condition to eliminate bone marrow and blood cell; 2. defatting with 95-100 wt% concentration ether solution and rinsing with aseptic water to eliminate ether; 3. ultralow temperature freezing in liquid nitrogen at normal pressure; and 4. irradiating with gamma ray.

The invention belongs to the technical field of intelligent materials, relating to an electrochromism composite fiber. The composite fiber comprises a hollow carbon nano tube; polydiacetylene is formed in the carbon nano tube, when current is not led into the two ends of the composite fiber, the composite fiber presents the first color; when the current led into the composite fiber exceeds a critical value, the color of the composite fiber changes into the second color; and when the current led into the two ends of the composite fiber is turned off, the color restores into the first color. The preparation method of the composite fiber comprises the following steps: preparing a carbon nano tube erect column by a chemical vapor deposition method, and spinning into the carbon nano tube fiber; and dipping diyne monomer to the carbon nano tube fiber, and irradiating by utilizing ultraviolet rays, X-ray and gamma-ray to polymerize the diyne monomer into the carbon nano tube/polydiacetylene compound fiber. The electrochromic effect of the compound has repeat reversible comprehensive electrochromic performance, and has wide application potency and simple preparation method.

The invention discloses a preparation method of irradiation-resistant glass fiber air filter paper. The air filter paper is prepared from a uniform mixture of glass microfiber cotton, organic fibers and alkaline-free glass fiber short-cut filaments through a wet process. The mass ratio of the glass microfiber cotton, the organic fibers to the alkaline-free glass fiber short-cut filaments is 15-20:1-2:3-6. In the irradiation-resistant glass fiber air filter paper, the contents of K<+>, Na<+> and B<3+> are reduced, so that the irradiation resistance of the glass fiber air filter paper is improved and the glass fiber air filter paper has tolerance against [gamma] ray irradiation being not more than 2.5*10<4> Gy/h in dose rate and being not less than (6-6.5)*10<5> Gy in total dose. By means of addition of a silicon-series or a fluoro-series water repellent, the glass fiber air filter paper is significantly improved in hydrophobicity, irradiation resistance, tensile strength and the like, improves adaptability of nuclear-grade HEPA filter under a moisture environment and has a wider application range.

The invention provides an extraction process of low-molecular-weight fucoidan, and the obtained product is low in molecular weight (400-500Da), is high in purity and has proper good liquidity. The extraction process comprises the following steps: firstly, extracting kelp with water to prepare a leaching solution, carrying out repeated freeze-thawing treatment on the leaching solution, using alpha-amylase to remove starch, carrying out an alcohol precipitation step to obtain polysaccharide powder, crushing the polysaccharide powder into nano powder, then redissolving the nano powder in water, and carrying out coupling degradation by virtue of ferric trichloride and irradiation of gamma rays and electron beams to obtain the low-molecular-weight fucoidan.

The present invention relates to a process for preparing skin removed from a human donor, removal of cellular components and sterilizing the decellularized skin. The process comprises the following steps:

• • (1) decellularizing the skin including soaking the tissue in a detergent and rinsing same with sterile water;
  • (2) sterilizing the skin with electronic beam irradiation, gamma irradiation or ultraviolet light for a time period to achieve a concentration to maximize sterilization; and
  • (3) processing the tissue by cutting the tissue to a designated size.

A method for the production of a cross-linked support matrix that in form of a bed will permit liquid flow velocities above 5 cm/h, preferably above 50 cm/h, to pass through the bed. The method is characterized in that it comprises the steps: (a) providing a starting support matrix that has pendent unsaturated groups, and (b) subjecting said starting support matrix to electron beam or gamma-ray irradiation. The use of a cross-linked support matrix produced by a method comprising the steps of: (a) providing a starting support matrix that has pendent unsaturated groups, and (b) subjecting said starting support matrix to electron beam or gamma-ray irradiation as a support matrix in liquid chromatography, cell culturing, step-wise solid phase synthesis of organic compounds, running catalytic reactions by the use of a solid phase bound catalyst.

The invention relates to a neutron detector low current amplification device capable of achieving background compensation. The neutron detector low current amplification device comprises a self-powered neutron detector, an armored signal cable and a shielding cable and further comprises a signal current conversion amplification module used for converting current signals into voltage signals and a compensating current conversion amplification module. The neutron detector low current amplification device further comprises a subtraction processing module used for obtaining two amplitude subtracting signals of two voltage signals and an amplitude adjustment module for adjusting the voltage signal amplitude. The invention further relates to a neutron detector low current amplification method capable of achieving background compensation. The method includes the steps of collecting current signals of an emitter and current signals of a background core wire, converting the current signals into voltage signals, performing amplitude subtracting on the voltage signals, and finally obtaining final voltage signals corresponding to neutron flux. According to the neutron detector low current amplification device and method capable of achieving background compensation, the influence, generated by gamma ray irradiation, on currents is eliminated; accurate wide-range measurement of neutron currents of the self-powered neutron detector can be achieved; in the neutron detector low current amplification device and method capable of achieving background compensation, the accurate measurement range of neutron currents can reach 10-11 A to 10-5 A.

The invention belongs to the technical field of medicine, and relates to application of indolepopionic acid to preparation of drugs for preventing and/or treating tumor radiotherapy side effect. Through research, the fact that indolepopionic acid can obviously improve intestinal injury of mice, caused by gama ray irradiation, is found, the improvement shows that the survival rate of laboratory mice is obviously improved, the weight is risen again, the intestinal inflammation level is obviously reduced, and paradoxical reaction of the laboratory mice does not occur, the fact that indolepopionicacid can be effectively applied to the prevention and/or treatment of tumor radiotherapy side effect is shown, and the indolepopionic acid is low in toxic and side effect, and effective before and after being taken, and has a wide application potential.

To provide a spinal intervertebral disk replacement material for nucleus pulposus which is relatively superior in load resistance that causes smaller damage of the anulus fibrosus by insertion during operation, remains in the anulus fibrosus stably after operation, and also allows prevention of the damage of the vertebral body cartilage endplate and the anulus fibrosus surrounding the prosthetic portion by sliding, and a production method thereof. The present invention relates to a spinal intervertebral disk replacement material for nucleus pulposus, comprising a dry polyvinylalcohol gel having a percentage of saturated water content of 18 to 50 wt % that changes its shape at a shape deformation rate of 150 vol % or more by water absorption. The present invention also relates to a method of producing the spinal intervertebral disk replacement material for nucleus pulposus above, comprising a step of preparing a polyvinylalcohol gel having a percentage of saturated water content of 20 to 53 wt %, a step of converting the polyvinylalcohol gel into a polyvinylalcohol hydrogel in the hydrated state and forming it into a shape having a hollow region, a step of irradiating the polyvinylalcohol hydrogel with γ ray at an intensity of 20 to 100 kGy, and a step of drying the PVA hydrogel in such a manner that the resulting hydrogel is so deformed that the hollow region disappears.

The invention discloses a natural honey wound dressing and a preparing method and application thereof. The natural honey wound dressing is prepared from honey and nystain, and the concentration of the nystain ranges from 10 mg/L to 50 mg/L. The nystain is added into the natural honey wound dressing, when the dressing acts on a wound, the growth of fungi in the honey can be inhibited by the nystain, and thus new infection of the wound is avoided. Meanwhile, the preparing method of the natural honey wound dressing is simple and easy to implement, the 60 Co-gamma-ray irradiation step which is difficult to implement is omitted, the fungus inhibiting effect achieved through 60 Co-gamma-ray irradiation can be achieved, and remarkable economical benefits are achieved.

The invention discloses a separation and preparation method of deer serum. According to the method, the deer whole blood obtained after sterile blood sampling from a cleaned and disinfected to-be-sampled deer is left to stand and subjected to centrifugal separation, fibrous protein is removed, then the whole blood is inactivated, the concentration of IgG and the content of glucose are reduced, obtained serum is irradiated with a certain dosage of gamma-rays, viruses and mycoplasmas are removed, the serum is irradiated with ultraviolet rays for sterilization while being filtered by an asbestos-free Chua's filter disc, deer serum filtrate is obtained, finally, the deer serum filtrate is filtered with a filtering membrane, and a finished product, the deer serum, is obtained. The preparation method is simple, convenient, quick, stable in process and suitable for large-scale production; the obtained deer serum does not contain fibrous protein, the concentration of IgG and the content of glucose are low, pollution of the viruses, germs and mycoplasmas can be completely eliminated, and the prepared deer serum can be used for preparation of a culture medium containing serum cells.