Natural honey wound dressing and preparing method and application thereof

A technology of natural honey and wound dressing, applied in the field of biomedicine, can solve the problems of wound infection, high cost of instruments, loss of honey wound dressing to treat wounds, etc., and achieves the effects of avoiding infection, a simple and easy preparation method, and significant economic benefits.

Inactive Publication Date: 2015-12-23
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in order to kill microorganisms in honey, currently, medical-grade honey wound dressings generally require high-dose 60 Sterilization by Co-gamma irradiation, but at high doses 60 Co-γ-ray irradiation sterilization conditions are only available in large professio

Method used

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  • Natural honey wound dressing and preparing method and application thereof
  • Natural honey wound dressing and preparing method and application thereof
  • Natural honey wound dressing and preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0032] Specific steps are as follows:

[0033] Step 1, preparing LB liquid culture medium (containing tryptone 10g / L, yeast extract 5g / L, NaCl10g / L, prepared with water, mixed evenly and then autoclaved to obtain final product);

[0034] Step 2, adding 100 μL of Escherichia coli BL21 (DE3) bacterium liquid into the LB liquid medium prepared in 10 mL step 1 (Escherichia coli BL21 (DE3) bacterium liquid: LB liquid medium=1:100, v / v), Shake the bacteria at 200 rpm at 37°C for 10-12 hours to obtain the E. coli liquid;

[0035] Step 3. Dilute the natural honey (the natural honey produced by Apis mellifera that the beekeeper obtains) with the LB liquid culture medium prepared in step 1 to obtain a honey concentration (volume percentage) of 80%, 70%, 60% %, 50%, 40%, 30%, 20%, 10%, and 5% of a series of honey samples, and another LB liquid medium was taken as a honey sample whose honey concentration (volume percentage) was 0%, that is, the control Group;

[0036] Step 4: Add 20 μL...

experiment example 2

[0065] Use the wound dressings of Example 2 and Comparative Example 1 in Table 1 and the wound dressings of Comparative Example 4 (the size of the gauze is equivalent to the size of the wound) to apply to wounds with moderate to large amounts of exudate (24h wound exudate is less than 5mL) A small amount; 5-10mL is a moderate amount; more than 10mL is a large amount). After 72 hours, the wound exudate was taken by the ten-point method on the wound surface and observed under the oil immersion immersion microscope, and it was found that only the wound exudate of the wound dressing of Example 2 had no fungal growth, and the wound exudate of the wound dressing of Comparative Example 1 and Comparative Example 4 was used Fungi grow and reproduce. It can be seen that the natural honey wound dressing of the present invention can also inhibit the growth of fungi and prevent new infection of the wound without sterilizing by radiation.

[0066] Apply the same method to the wound with a ...

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Abstract

The invention discloses a natural honey wound dressing and a preparing method and application thereof. The natural honey wound dressing is prepared from honey and nystain, and the concentration of the nystain ranges from 10 mg/L to 50 mg/L. The nystain is added into the natural honey wound dressing, when the dressing acts on a wound, the growth of fungi in the honey can be inhibited by the nystain, and thus new infection of the wound is avoided. Meanwhile, the preparing method of the natural honey wound dressing is simple and easy to implement, the 60 Co-gamma-ray irradiation step which is difficult to implement is omitted, the fungus inhibiting effect achieved through 60 Co-gamma-ray irradiation can be achieved, and remarkable economical benefits are achieved.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a natural honey wound dressing and its preparation method and application. Background technique [0002] Honey is a complex and nutrient-rich supersaturated sugar solution with extremely high osmotic pressure and low pH value, containing hydrogen peroxide (H 2 o 2 ) and methylglyoxal (methylglyoxal, MGO) and other bactericidal ingredients have broad-spectrum antibacterial activity, and their antibacterial activity has no risk of drug resistance. [0003] Honey is widely used in wound treatment due to its broad-spectrum antibacterial activity and resistance to drug resistance. Processes such as debridement and tissue growth promote wound healing. Honey was first used in wound treatment in 1988 (EfemSE. Clinical observation on the wound healing properties of honey. BrJSurg, 1988, 75 (7): 679-681.), what was used at that time was unprocessed honey, through the treatment of 59 cases of ...

Claims

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Application Information

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IPC IPC(8): A61L15/40A61L15/28A61L15/44
Inventor 耿爱香张成岗徐向英刘英男李军怀
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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