Streptococcus equi subsp. Zooepidemicus subspecies SXY36 and application in fermentation production of hyaluronic acid

A technology of Streptococcus equi and hyaluronic acid, which is applied in the direction of fermentation, microbial-based methods, bacteria, etc., can solve the problems of limited application, fermentation cost and HA production, and achieve high fermentation yield and genetic stability

Active Publication Date: 2017-11-10
株洲市中建新材料有限公司
View PDF4 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows creation of synthetic polysaccharides called hyaluronan (HA) on an industrial scale through microbial processes such as bacteria or yeast cells grown inside stainless steel tanks at specific temperatures. These techniques help increase production efficiency while maintaining consistently good quality products like those made from traditional methods.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the quality and quantity of naturally occurring α -hydroxy acids such as glucose monomers and their derivatives like lactic acid, polyasparagine, collagen, gelatin, alginate, and others. These natural substances have specific structures and sizes depending upon various needs and uses. By combining these components together they may provide improved performance characteristics compared to existing products containing only either single component compounds alone. Additionally, producing smaller particles of crystalline hydrocanaphosphorus sulfone diester hexacyanohepatotoxin type II A hemihydrosulfite adduct ion complex, known as liver sinceroid protein-2 dimmers, could potentially improve absorption rates when administered intravenously without causing harmful side effects.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Streptococcus equi subsp. Zooepidemicus subspecies SXY36 and application in fermentation production of hyaluronic acid
  • Streptococcus equi subsp. Zooepidemicus subspecies SXY36 and application in fermentation production of hyaluronic acid
  • Streptococcus equi subsp. Zooepidemicus subspecies SXY36 and application in fermentation production of hyaluronic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: mutagenesis of Streptococcus equi subspecies zooepidemicus SXY36 strain

[0026] The HA fermentation yield of Streptococcus equi subsp. It carries out continuous mutagenesis, and selects high-yielding strains from the mutants.

[0027] (1) UV mutagenesis

[0028] Prepare the bacterial suspension of the starting strain GIM1.437, after mutagenesis by ultraviolet radiation, the bacterial liquid is diluted and spread on a plate medium for culture, pick a single colony and transfer it to the slant medium to obtain the strain, and then insert the bacteria of each strain Shake flask culture medium was cultivated at 37°C and 200r / min for 24 hours, and the HA content was detected. The strains with HA production rate increased by more than 10% were screened out initially, and then subcultured and re-screened to obtain mutant strains with significantly increased HA fermentation production rate.

[0029] The ultraviolet mutagenesis method is as follows: turn on the ...

Embodiment 2

[0062] Embodiment 2: the method for producing HA by SXY36 strain shake flask fermentation under optimal conditions

[0063] Using the SXY36 strain as the strain, after optimizing the composition of the fermentation medium and the fermentation conditions, the yield of HA produced by shake flask fermentation was significantly improved. The specific steps are as follows:

[0064] (1) The SXY36 strain stored in frozen glycerol tubes was inoculated on the slant medium, and cultured at 37° C. for 24 hours to obtain the activated slant of the SXY36 strain. The final concentration of the slant medium consists of: glucose 2g / L, beef extract 5g / L, peptone 5g / L, yeast extract powder 2g / L, K 2 HPO 4 2.5g / L, MgSO 4 1.5g / L, agar 20g / L, solvent is distilled water, pH is 7.0.

[0065] (2) Inoculation loop picking step (1) After activation culture, 2 loops of SXY36 bacteria were inoculated into 100mL seed medium, cultured at 37°C and 200r / min shaking for 24 hours, and the concentration of d...

Embodiment 3

[0067] Embodiment 3: the method that SXY36 strain produces hyaluronic acid in fermentor

[0068] Using the SXY36 strain as the strain, use a 10L mechanically stirred fermenter to ferment and produce hyaluronic acid. The specific steps are as follows:

[0069] (1) SXY36 cells stored in frozen glycerol tubes were inoculated on the slant medium, and cultured at 37° C. for 24 hours to obtain the activated slant of the SXY36 strain. Described slant culture medium composition and preparation method are with embodiment 2;

[0070] (2) Inoculation loop picking step (1) After activation culture, 2 loops of SXY36 cells were inoculated into 3 bottles of 100mL seed medium, cultured at 37°C and 200r / min shaking for 24h, and the concentration of dry cells was 1.37 g / L seed liquid; described seed medium composition and preparation method are with embodiment 2;

[0071] (3) Transplant the seed solution of step (2) into 6L fermentation medium with an inoculum volume concentration of 2%, that...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a Streptococcus equi subsp. Zooepidemicus subspecies SXY36 and an application in the fermentation production of hyaluronic acid. The SXY36 strains of Streptococcus equi subsp. Zooepidemicus subspecies and a method for controlling HA molecular weight using the fermentation process are provided. The method has the advantages that SXY36 is subjected to irradiation mutation breeding by ultraviolet rays, microwave and gamma rays, no hemolysis exists, the HA fermentation yield is high, and the heredity is stable, in the optimal fermentation condition, the fermentation yield of shaking flask is up to 0.731 g/L, and the yield of fermentation by a fermentation cylinder can reach 4.78 g/L; a two-stage variable temperature fermentation and stable pH method is adopted, high molecular-weight HA can be prepared by fermentation, and the molecular weight can reach 1.89 MDa, which is two times the molecular weight by a conventional fermentation method; HA with the molecular weight in the range of 0.035-1.89 MDa can be produced by using a fermentation broth self-enzymatic degradation method to prepare different molecular-weight HA and by controlling the time of enzymatic hydrolysis.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner 株洲市中建新材料有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap