6results about How to "No hemolytic" patented technology

The present application relates to the technical field of microorganism, and especially relates to Enterococcus hirae C01, probiotic agent and application thereof.The present application provides the Enterococcus hirae C01, and the preservation number is CGMCC No.33994.The strain has broad-spectrum bacteriostatic activity, can inhibit Salmonella enteritidis, Salmonella pullorum and Salmonella typhimurium, has no hemolytic activity, has good stress resistance, can survive in gastrointestinal environment, and has high biological safety, and can be used for preventing and controlling mixed Salmonella infection in clinic.

This invention discloses a strain of *Lactobacillus johnsonii* DA0207 and its applications, belonging to the field of microbial technology. The *Lactobacillus johnsonii* DA0207 provided by this invention was isolated from the enteric coating of *Deinagkistrodon acutus*, and can grow well on MRS agar medium. It is non-hemolytic, sensitive to a variety of common antibiotics, and has good safety. This strain has good acid and bile salt tolerance, can adapt to the intestinal environment of humans and animals, and has growth inhibitory effects on a variety of common pathogenic bacteria. Fermentation of *Periplaneta americana* powder with this strain significantly increased the content of various amino acids and peptide active compounds in the fermentation supernatant. The supernatant showed growth inhibition and apoptosis-promoting effects on colon cancer cells. The supernatant also showed significant anti-inflammatory effects on DSS-induced colitis in mice, significantly reducing the levels of inflammatory factors. This provides a new avenue for the further development and utilization of functional foods, biological agents, and *Periplaneta americana*.

The invention provides an antibacterial peptide mutant and application thereof. The amino acid sequence of the antibacterial peptide mutant is as shown in SEQ ID NO: 1. The nucleotide sequence codes the antibacterial peptide mutant, and the nucleotide sequence is as shown in SEQ ID NO: 2. The antibacterial peptide mutant provided by the invention can resist heat up to 120 DEG C, and can resist extreme strong acid and strong alkali environments with pH of 1-13 at normal temperature. The protein has stable physiological and biochemical properties, can tolerate hydrolysis of pepsin and trypsin with certain concentration, and has no hemolytic activity. The mutant of the Mccj25 antibacterial peptide provided by the embodiment of the invention has good bactericidal activity, can reduce the use cost of the antibacterial peptide to a certain extent, reduces the addition amount of the antibacterial peptide, and has a good application prospect.

This invention relates to the field of food antibacterial and preservative technology, specifically to a plant lactobacillus 9, a compound preservative containing plant lactobacillus 9, and their applications. The plant lactobacillus 9 is produced from *Lactobacillus plantarum* with the preservation number CCTCC NO: M 2026808. Lactobacillus plantarum The amino acid sequence of this plant lactobacillus 9 is N-Thr-Asp-Gly-Ala-Ala-Phe-OH. This plant lactobacillus 9 exhibits strong antibacterial activity against Listeria monocytogenes, Bacillus subtilis, Micrococcus luteus, methicillin-resistant Staphylococcus aureus, Escherichia coli, Pseudomonas fluorescens, Salmonella typhimurium, and Pseudomonas aeruginosa, and demonstrates outstanding stability. It is a safe and reliable biological preservative. Its effectiveness is significantly enhanced when combined with chitosan, making it a potential alternative to chemical preservatives for controlling foodborne pathogens in the agricultural and food industries and extending the shelf life of meat and meat products.

The invention discloses a complex microbial inoculant as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The complex microbial inoculant disclosed by the invention is prepared by co-fermentation of lactobacillus plantarum CGMCC (China General Microbiological Culture Collection Center) NO.12849 and enterococcus faecium CGMCC The complex microbial inoculant has the characteristics of high antibacterial activity, safety, no toxicity, high stability and the like, and can be used for killing clinically common gram-negative pathogenic bacteria. By utilizing the high-density fermentation culture medium, the fermentation process and the preparation process provided by the invention, the complex microbial inoculant can be produced on a large scale and is low in cost, and the complex microbial inoculant is expected to replace antibiotics to become a safe, green and efficient ideal antibacterial agent and has a very wide application prospect.

The present application relates to a strain of Bacteroides fragilis and its application, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 14, 2026, and the preservation number is: CGMCC No. 38257.The strain can regulate STING-dependent training immunity, effectively resist Salmonella typhimurium and H9N2 avian influenza virus infection, maintain the integrity of the intestinal barrier of the host, and reduce inflammatory damage.The Bacteroides fragilis of the present application has good biological safety, and the strain can be used as a training immunity inducer, and the strain is expected to be a candidate probiotic or vaccine adjuvant for preventing / treating bacterial and viral infections, suitable for human health and poultry farming, and provides a new solution to resist antibiotic resistance.