63 results about "Cathelicidin antimicrobial peptide" patented technology

Antimicrobial peptides and methods for their use are provided. The peptides are optimized, truncated versions of chicken cathelicidins (“fowlicidins”).

The invention relates to antimicrobial cathelicidin polypeptides related to a 38 amino acid peptide having SEQ ID NO:4. The invention provides for polypeptides having broad spectrum antimicrobial activity, nucleic acids and expression vectors encoding such polypeptides, as well as host cells and methods of reducing survival of a microbe. In addition, the invention also provides compositions, as well as articles of manufacture, that comprise a broad spectrum antimicrobial polypeptide.

The invention relates to the field of biomedicine and in particular relates to an antibacterial polypeptide. Part of active amino acids of the antibacterial polypeptide of Cathelicidin family is mutated based on the antibacterial polypeptide of Cathelicidin family, and then the antibacterial polypeptide with high inhibitory activity to penicilin-resistant bacterium is obtained by solid phase chemical synthesis. The result of pharmacodynamic tests shows that the antibacterial polypeptide provided by the invention has good in vitro antibacterial activity and is applicable to treating diseases about drug-resistant bacterial infection.

The invention relates to defensin cathelicidin-PP of polypedates puerensis as well as a gene and application thereof, and belongs to the field of biomedicine. The defensin cathelicidin-PP is a cyclic polypeptide encoded by a gene of defensin of Chinese amphibian polypedates puerensis, and has a molecular weight of 3,366.08 daltons and an isoelectric point of 10.05; the amino acid sequence of the defensin cathelicidin-PP is as shown by SEQ ID NO: 1. A gene GenBank Accession KY610282 for encoding a precursor of the defensin cathelicidin-PP of the polypedates puerensis consists of 615 nucleotide sequences; the nucleotide sequence of the gene GenBank Accession KY610282 is as shown by SEQ ID NO: 2, wherein nucleotides at No. 346 to No. 441 sites are encoding genes of defensin cathelicidin-PP of mature polypedates puerensis. The invention relates to the application of the defensin cathelicidin-PP of the polypedates puerensis to the preparation of a therapeutic medicine for infectious diseases caused by escherichia coli, salmonella paratyphi A, pseudomonas aeruginosa and candida glabrata. The defensin cathelicidin-PP has the obvious effects of inhibiting the growth of a bacterium and a fungus for the escherichia coli, the salmonella paratyphi A, the pseudomonas aeruginosa and the candida glabrata.

The invention discloses children antimicrobial peptides cathelicidins, a preparation method and the applications thereof. The nucleotide sequence and the amino acid sequence of children antimicrobial peptides Cathelicidin1, Cathelicidin2 and Cathelicidin3 are shown as a sequence table. The preparation method of the antimicrobial peptides adopts a solid-phase chemical synthesis method or a geneticengineering method. The genetic engineering method comprises the following steps of: respectively cloning the encoding genes of mature children antimicrobial peptides Cathelicidin1-3 to expression vectors; and then, guiding into host cells to express. By applying the genetic engineering technology in the invention, the children antimicrobial peptides Cathelicidins are expressed in the prokaryotichost cells with high efficiency. Proved by experiments, the antimicrobial peptides have obvious inhibition on various gram-positive bacteria and negative bacteria.

Agents and natural and synthetic formulations and extracts are useful in control of pathogen infection and infestation in humans, animals and plants, as well as pathogen contamination in environmental locales. A method inhibits growth or infection or infestation of a pathogen, including contacting the pathogen with an effective amount of a combination of a plant defensin or a functional natural or synthetic derivative or variant thereof and a non-defensin peptide, such as a cathelicidin peptide, a hairpin peptide, a hairpin peptide from which disulfide bridges have been removed, indolicidin, synthetic peptide CP-29, or a peptide derived from a cystatin. The combination of the defensin and peptide are synergistic compared to the use of each alone at the same individual dose as used in the combination.

The invention discloses [delta]Tlrgt2 trichoderma engineering bacteria with high yield of peptaibols and a construction method and application thereof. A Tlrgt2 knockout vector is constructed by upstream and downstream homologous arm sequences of a Tlrgt2 gene and a hygromycin resistance gene hph sequence, the [delta]Tlrgt2 trichoderma engineering bacteria is prepared by a gene knockout method, aTlrgt2 gene is knocked out, and the peptaibols production capacity of the bacteria can be effectively improved. The stable passage of the constructed [delta]Tlrgt2 trichoderma engineering bacteria canbe achieved, the yield of the trichoderma peptaibols can be significantly increased, and the production of the peptaibols by the [delta]Tlrgt2 trichoderma engineering bacteria is about 12 times higher than that of an original strain. The application of the trichoderma peptaibols in medicine and agriculture is facilitated.

The invention discloses canine chewing gum with an oral care coating. The canine chewing gum comprises an inner core and the oral care coating for externally covering the surface of the inner core. The inner core is prepared by amylose, an edible binder, grease, insoluble cellulose, soluble dietary cellulose, a humectant, and a flavor agent. In solution of the oral care coating, a mass percentageof an effective component is 0.1-5%, and the rest is water. The effective component is a functional component and an auxiliary component. The functional component is formed by a persimmon extract, a grapefruit seed extract, a honeysuckle extract, a cranberry extract, a brown seaweed extract, a green tea extract, a yucca extract, tricortin, an antibacterial peptide, lysozyme, a negative ion salt vitamin, propolis extracting solution, calcium gluconate, polyphosphate, water-soluble silicon, and tea polyphenol; and the auxiliary component is arbutin. The canine chewing gum is capable of, in allusion to defects of an existing product, covering the surface of the inner core without water absorption or with slow water absorption by the oral care coating, and developing a multi-layer care effect.

The invention discloses a high-moisture facial mask based on cellulose fibers and a preparation method of the high-moisture facial mask. The moisturizing facial mask comprises a modified facial mask base cloth and a facial mask base solution, and the facial mask base solution comprises the following raw materials in parts by weight: 8 to 10 parts of sodium hyaluronate, 8 to 12 parts of carbomer, 6to 8 parts of antibacterial peptide, 3 to 4 parts of N,N-dicyclohexylcarbodiimide, 10 to 15 parts of triethanolamine, 8 to 10 parts of glycerinum, 3 to 5 parts of collagen, 4 to 7 parts of sodium methylparaben and 6 to 8 parts of a traditional Chinese medicine additive, w herein the modified facial mask base cloth is prepared by treating a facial mask base cloth with isopropanol, sodium hydroxide, tetramethylammonium hydroxide, epoxypropane and glucose oxidase. The process design is reasonable, the component ratio is suitable, the prepared moisturizing facial mask has excellent effects of water supplementing, moisturizing and skin moisturizing, further has an excellent antibacterial effect, can inhibit the microbial growth on skin surfaces and avoid acne occurrence, and has better practicality.

The invention relates to a Brachymystax lenok Cathelicidin antimicrobial peptide CATH_BARLE, and a gene, a preparation and an application thereof, and belongs to the technical field of biomedicine. The gene of the CATH_BARLE is composed of 909 nucleotides, and a part coding the mature peptide is nucleotides from the 439th bit to the 597th bit. The mature peptide CATH_BARLE is rich in alkaline amino acids, and has a strong bactericidal effect on common pathogens in aquatic product culture. The CATH_BARLE has a simple structure, does not contain a disulfide bond or an annular structure, and is convenient for chemical synthesis and gene engineering preparation; and the CATH_BARLE also has a good killing effect on many clinical drug-resistant bacteria, especially fungi.

The invention belongs to the technical field of polypeptide drugs in biochemistry, and particularly relates to an application of tylototriton verrucosus skin repair peptide cathelicidin-TV2. The tylototriton verrucosus skin repair peptide cathelicidin-TV2 is obtained according to a conventional chemical synthesis method; the molecular weight of the tylototriton verrucosus skin repair peptide cathelicidin-TV2 is 1631.98Da, the isoelectric point is 11.02, and the primary structure of the complete sequence of the tylototriton verrucosus skin repair peptide cathelicidin-TV2 is RKCVRQNNKRVCK. The tylototriton verrucosus skin repair peptide cathelicidin-TV2 disclosed by the invention has the functions of reducing scar generated by skin trauma and accelerating the treatment or repair of scars, and can be applied to preparation of drugs for treating skin trauma, reducing scars and accelerating scar repair.

The invention discloses a comprehensive model for protecting mice and curing mice macrophage system UC (Ulcerative Colitis) by C-BF (Cathelicidin-BF). C-BF has broad-spectrum antibacterial activity, especially for gram-negative bacteria. IBDS (Inflammatory Bowel Diseases) include UC and CD (Crohn's Disease), and the pathological mechanisms of IBDS are not clear. The comprehensive model provided by the invention combines in-vivo mice experiments and in-vitro cell experiments to evaluate the effects of C-BF on relieving inflammations, reducing the cell apoptosis level and improving the intestinal barrier function.

The present invention provides new derivatives of CATH2 or CMAP27, one of the cathelicidins. These derivatives comprise N-terminally truncated peptides, cyclic peptides, D-amino acid variants of CATH2 and its truncated derivatives, inverso and retro-inverso CATH2 derivatives. These derivatives are useful as anti-infectives, in vaccines, and especially for in ovo applications. Further, for the above derivatives and also for the already known C-terminally truncated derivatives new immunoactivating functions have been described that are particularly advantageous for prophylactic treatments.

A food processing equipment disinfectant consists of the following raw materials by weight: 3-7 parts of fatty alcohol polyoxyethylene ether, 3-7 parts of sodium carbonate, 4-6 parts of citric acid, 1.2 -2.4 parts of Neroli oil, 6-9 parts of Hibiscus mutabilis L., 9-14 parts of japanese stephania root, 1-4 parts of glutaraldehyde, 4-7 parts of iodoanisole, 3-6 parts of zinc acetate, 1.2-2 parts of Tween, 2-4 parts of antimicrobial peptides, 1.5-3 parts of a thickener 2-3 parts of potassium iodide and 13-16 parts of distilled water. The invention has the beneficial effects that the food processing equipment disinfectant has excellent sterilization and disinfection effects, no harm to equipment and operating workers and good durability.

A polytherapy of orally available compounds is disclosed that synergistically modulates and induces the expression of the cathelicidin gene (CAMP), which encodes the host defense peptide LL-37. By providing a number of different CAMP-inducing compounds together at the same time, stronger gene induction is achieved than with just one or two compounds, because the mechanism of induction broadens. Induction also may vary in different parts of the body depending on which compounds are used, and at what levels. We show for the first time that the polytherapy can induce cathelicidin expression in the brain, which may help to treat or prevent Alzheimer's Disease. Systemic cathelicidin gene induction may help treat numerous other conditions including Type 2 Diabetes/Metabolic Syndrome, or chronic bacterial, viral, or fungal infections associated with increased cancer risk or neurodegeneration. By increasing cellular autophagy and macroautophagy and supporting mitochondrial biogenesis and homeostasis, CAMP gene upregulation may reduce the effects of cellular aging and increase longevity.

The invention provides a traditional Chinese medicine, an antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating porcine diarrhea as well as a preparation method and application. The traditional Chinese medicine for preventing and treating porcine diarrhea consists of the following raw materials in parts by weight: 50 to 60 parts of pilose asiabell root,50 to 60 parts of poria cocos, 50 to 60 parts of bran-stir-fried largehead atractylodes rhizome, 50 to 60 parts of rhizoma dioscoreae, 50 to 60 parts of stir-fried white hyacinth bean, 10 to 20 partsof fructus evodiae, 10 to 20 parts of stewed fructus chebulae, 10 to 20 parts of oriental waterplantain rhizome, 10 to 15 parts of halloysitum rubrum, 25 to 35 parts of lotus seed, 25 to 35 parts ofbran-stir-fried semen coicis, 15 to 25 parts of semen euryales, 10 to 20 parts of fructus amomi, 25 to 35 parts of radix platycodi, 25 to 35 parts of fructus schisandrae chinensis, 25 to 35 parts of pericarpium citri reticulatae, 25 to 35 parts of semen myristicae, 25 to 35 parts of parched fructus crataegi, 25 to 35 parts of parched medicated leaven, 25 to 35 parts of parched malt, 10 to 20 partsof endothelium corneum gigeriae galli, 15 to 25 parts of lesser galangal rhizome and 25 to 35 parts of liquorice root. The prevention and treatment effect is good, no toxic or side effects exist, andno medicine residue exists.