7 results about "Peptide Synthesis technique" patented technology

The application relates to the technical field of peptide synthesis, and discloses a preparation method of a snake venom-like tripeptide, which comprises the following steps: condensing Boc-beta-Ala-OH and H-Pro-OMe, hydrolyzing the methyl ester groups to obtain Boc-beta-Ala-Pro-OH; condensing Boc-Gln-OH and benzylamine, and removing Boc to obtain H-Gln-NHBzl; then condensing Boc-beta-Ala-Pro-OH and H-Gln-NHBzl to prepare a polypeptide; and after the Gln in the polypeptide is subjected to Hofmann rearrangement, the Boc protection group in the polypeptide is removed to obtain the snake venom-like tripeptide. The preparation method adopts liquid-phase synthesis of the snake venom-like tripeptide, the synthesis route is short, the reaction condition is mild, special equipment and reagents are not needed, the operation steps are simplified, the process cost is reduced; the reaction reagents used are cheap and easy-to-obtain industrial reagents, the raw material cost is reduced; and the product purity can reach more than 99%.

The application discloses a semi-chemical synthesis method of an N-terminal domain of matrix metalloproteinase inhibitor-2 (N-TIMP2) and application thereof. The N-TIMP2 is divided into three fragments, wherein a key tyrosine post-translational modification site is located in the fragment 2; the polypeptide resins of the fragment 1 and the fragment 2 are synthesized by using a solid-phase polypeptide synthesis technology, and the fragment 1 and the fragment 2 with a C-terminal hydrazide are obtained after cleavage, deprotection, extraction and purification; the fragment 3 is obtained by combining an E. coli recombinant expression technology and a three-step one-pot selective protection reaction of side chains of mercapto; the fragment 1, 2 connecting product is obtained through a first-step natural chemical connecting reaction; the full-length linear protein is obtained through a second-step natural chemical connecting reaction, desulfurization and deprotection; and finally, the N-TIMP2 protein is obtained through a refolding reaction. The semi-chemically synthesized N-TIMP2 protein has a comparable MMP-14 inhibiting activity to the N-TIMP2 protein obtained by using the E. coli recombinant expression technology.

The invention discloses a solid-phase synthesis method of Mortudotide, belongs to the technical field of polypeptide synthesis, and particularly relates to a method for synthesizing Mortudotide full-protection peptide resin by adopting a solid-phase synthesis method, then treating the Mortudotide full-protection peptide resin by adopting a cracking reagent to prepare crude Mortudotide. According to the present invention, during the chromatographic purification of the Macerdotide crude product peptide, the chromatographic column can be adopted to obtain the Macerdotide pure product peptide, the trithioacetone can be added to the cracking reagent, and the yield and the purity of the Macerdotide can be improved by using the trithioacetone. The solid-phase synthesis method of Macervide has the advantages of high yield, high purity, reduction of [-Tyr] and [-Thr] deletion impurities, and reduction of [+ Gly] increase impurities.

The invention provides a solid-phase synthesis method of tilpotitide, and belongs to the technical field of polypeptide synthesizing.According to the preparation method, a small amount of lysate is adopted for lysing, a small amount of poor solution composed of methyl tert-butyl ether and n-heptane with the volume ratio of 1-2: 1 is used for crude peptide precipitation, precipitated particles are uniform and large and are not prone to caking, and the solid-phase synthesis method is suitable for industrial production. Compared with the prior art, the method has the advantages that the crude peptide impurity level is lower, the subsequent purification step is easy, the amount of generated waste liquid is small, the activating agent 7-(1H-pyrrolo [2, 3-b] pyridine-1-yl)-1H-benzo [d] [1, 2, 3] triazole-1-alcohol is used for activating amino acid, the coupling reaction degree is high, and the target peptide yield is high. The solid-phase synthesis method of the tilpotide has the advantages of being simple, economical, capable of achieving mass production, low in impurity content, high in purification yield, high in yield and the like.

The invention relates to a method for preparing Fmoc-Lys (R)-OH fragment peptide by a solid phase method, and belongs to the technical field of peptide synthesis. According to the method, a solid-phase synthesis process route is adopted, 2-CTC lipid is taken as a solid-phase carrier, an initial material Dde-Lys-FMOC is adopted, any amino acid can be coupled to side chain amino groups in sequence, after coupling is completed, hydrazine hydrate is adopted for reduction removal of Dde and FMOC-0su cap exposure of amino groups, and finally, weak acid is adopted for cutting off full-protection peptide from resin, so that a target product crude peptide is obtained. The purity of the crude peptide prepared by the method reaches 95%, the purity can reach 99% or above through one-step extraction, the method avoids the problems of long liquid phase synthesis time and tedious procedures, and the product yield and the process stability are greatly improved.

This invention discloses a rhodamine B-modified lysine derivative, its synthesis method, and its applications. The structural formula of the rhodamine B-modified lysine derivative is shown below. The invention first synthesizes Fmoc-Lys(Boc)-OtBu using commercially available Fmoc-Lys(Boc)-OH as a starting material. Then, after selectively removing the Boc protecting group from the side chain amino group, it condenses with rhodamine B to generate Fmoc-Lys(Rho)-OtBu. Finally, the tBu protecting group is removed to obtain Fmoc-Lys(Rho)-OH. The Fmoc-Lys(Rho)-OH synthesized by this invention can be loaded onto peptides using Fmoc solid-phase peptide synthesis technology for rapid and automated synthesis of rhodamine B-modified peptides.

The invention discloses a liquid-phase synthesis method of acyl cyclic ester peptide antibiotic Cilagicin, which is prepared and synthesized through a label-assisted liquid-phase synthesis strategy capable of synthesizing cyclic peptide in batches, and compared with the traditional solid-phase polypeptide synthesis and liquid-phase polypeptide synthesis technologies, the liquid-phase synthesis method has the advantages that the use of amino acid raw materials and chemical reagents can be reduced to the maximum extent, and the cost is reduced. And chemical wastes such as polymer resin are prevented from being discharged.