Method for synthesizing phenylalanyl-lysine dipeptide

Abstract

A method for synthesizing phenylalanyl-lysine dipeptide belongs to the technical field of peptide synthesis. The method comprises the following steps: preparing an aqueous solution of phenylalanine and lysine from commercially available phenylalanine and lysine, preparing a non-aqueous reaction medium of ethylene glycol, preparing a phenylalanine-lysine dipeptide reaction solution, preparing a resin column for purifying loaded phenylalanine-lysine dipeptide, preparing a phenylalanyl-lysine dipeptide eluent, preparing phenylalanyl-lysine dipeptide freeze-dried powder, preparing an aqueous solution of recovered phenylalanine and lysine, and preparing a regenerated resin column in order to prepare the phenylalanyl-lysine dipeptide with the content being 95-98%. The method using chymotrypsin as a catalyst in the non-aqueous medium of ethylene glycol to synthesize the phenylalanyl-lysine dipeptide has the advantages of mild conditions, high specificity and high synthesis efficiency, and isa typical green production technology; and the phenylalanyl-lysine dipeptide can be widely applied to the fields of medicines, textiles, foods and the like, and has considerable social values.

Description

1. Technical field

[0001] The invention belongs to the technical field of peptide synthesis, and in particular relates to a method for synthesizing phenylalanyl-lysine dipeptide. 2. Background technology

[0002] Short peptides are chain compounds composed of 2-9 amino acid residues. Many short peptides have important biological functions and are new members of biomedicine. They are also widely used in food, health products and daily chemicals. middle. For example, aspartyl-phenylalanine dipeptide is an important peptide sweetener, which is widely used in food, cosmetics and other fields; The decomposition of phenylalanine and lysine, phenylalanine is one of the essential amino acids, is the intermediate for the synthesis of amino acid anticancer drugs such as phenylalanine and formic acid sarcoma, and is also used for the production of adrenaline and thyroxine In the field of food, phenylalanine is one of the raw materials for the synthesis of health-care dipeptide sweete...