3158 results about "Lysine" patented technology

Aspects of the disclosure relate to compositions comprising a plurality of complexes comprising an antibody (e.g., anti-TfRI antibody) covalently linked to one or more oligonucleotides (e.g. a DMPK targeting oligonucleotide), each oligonucleotide being covalently linked at a linkage site represented by a lysine (K) residue of the antibody. In some embodiments, the antibody comprises a heavy chain comprising a heavy chain variable region (VH) and a heavy chain constant region, and a light chain comprising a light chain variable region (VL) and a light chain constant region, wherein at least 80% (e.g., 80%-98%, 80%-95%, 80%-90%, 85%-98%, 85%-95%, 85%-90%, 90%-98%, 90%-95%, 95%-97%, or more) of the light chain constant regions of the antibodies of the complexes in the composition are independently covalently linked to an oligonucleotide at a linkage site represented by K188 (based on Kabat numbering) and / or a linkage site represented by K190 (based on Kabat numbering) of the light chain constant regions of the antibodies.

The present disclosure describes pharmaceutically stable high concentration liquid formulations of antibody. Such formulations comprise, in addition to the antibody, at least one anti-aggregating agent selected from arginine or lysine, buffer and poloxamer 188. In addition, the present disclosure provides high concentrated antibody formulation having high monomer, low aggregates and desirable viscosity.

The invention discloses a phytophthora plant pathogenic oomycete histone lysine methyl transfer protein as well as a coding gene and application thereof. Wherein the phytophthora sojae histone lysine methyltransferase KMT3-1 is as shown in a sequence 2 in the specification, and the phytophthora sojae histone lysine methyltransferase KMT3-1 is as shown in a sequence 2 in the specification; the coding gene is shown as a sequence 1. Experiments prove that the KMT3-1 protein plays an important role in the growth and development process of phytophthora sojae, specifically, after the protein is deleted, the growth rate of phytophthora hyphae is slowed down, the sporangium yield is remarkably reduced, the release of zoospores is stopped, the oospore yield is reduced, the pathogenicity is reduced, and the like. Corresponding histone methyltransferase inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora sojae, phytophthora capsici and phytophthora infestans. The gene provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed on the basis of the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention belongs to the technical field of protein engineering, and discloses a Brazilian sweet protein mutant and application thereof. According to the invention, lysine is used for replacing aspartic acid at the 29th site of Brazilian sweet protein, and / or arginine is used for replacing glutamic acid at the 36th site, and / or alanine is used for replacing aspartic acid at the 50th site, amino acid mutation at the sites is carried out to construct a Brazilian sweet protein mutant, and the Brazilian sweet protein mutant is subjected to heterologous expression in pichia pastoris GS115, so that the Brazilian sweet protein mutant is obtained. And carrying out sweetness performance evaluation on the expressed Brazilian sweet protein mutant. The sweetness property of the double mutant Asp29Lys-Asp50Ala protein is improved by 240% compared with the sweetness property of the Brazilian sweet protein before mutation.

The invention discloses an antibacterial nylon material as well as a preparation method and application thereof, relates to the technical field of antibacterial high polymer materials, and solves the problems of poor antibacterial performance and difficulty in industrial production of existing antibacterial nylon 6. The preparation method comprises the following steps: mixing a seven-membered cyclic lysine derivative monomer with lactam, and carrying out hydrolytic polymerization by taking water as an initiator to obtain copolyamide; and placing the copolyamide in an acid solution, heating and stirring to carry out protonation reaction, and purifying the reaction product to obtain the antibacterial nylon material. The seven-membered cyclic lysine derivative monomer is an unprotected cyclic lysine monomer (aminocaprolactam), a single protected cyclic lysine monomer or a salified cyclic lysine monomer. The method can be widely applied to the fields with antibacterial requirements, such as fabrics, plates, pipes, packaging and various panels.

The invention discloses lysine cross-linked hyaluronic acid gel as well as a preparation method and application thereof. The preparation method of the lysine cross-linked hyaluronic acid gel comprises the following steps: mixing a hyaluronic acid substance, DMTMM and lysine, and carrying out a cross-linking reaction to prepare the lysine cross-linked hyaluronic acid gel, the hyaluronic acid substance is added in the form of a hyaluronic acid substance solution, and the solution comprises a hyaluronic acid substance with the weight-average molecular weight of 70WDa-180WDa and a buffer solution; the hyaluronic acid substance accounts for 3-5% of the hyaluronic acid substance solution in percentage by mass; the mass ratio of the DMTMM to the hyaluronic acid substance is (0.74-1.1): 1. The cross-linked hyaluronic acid gel is prepared by taking lysine as a cross-linking agent, so that the prepared gel is ensured to have ideal biocompatibility and use safety. The gel prepared by the method also has ideal mechanical properties, and the degradation speed meets the requirements of injection filling materials.

The invention discloses a high-performance carbonyl reductase mutant and application thereof in synthesis of a series of chiral alcohols. The high-performance carbonyl reductase mutant is obtained by performing the following mutations on an amino acid sequence as shown in SEQ ID NO.2: the 69th alanine mutates into any one of lysine, leucine, aspartic acid or asparagine, and the 69th alanine mutates into any one of lysine, leucine, aspartic acid or asparagine; and / or the 100th glycine is mutated into any one of glutamic acid, serine, lysine or asparagine. Compared with a wild type enzyme, the thermal stability and the catalytic activity of the obtained mutant are remarkably improved, particularly, single point mutants Mut-A69N, Mut-G100E and Mut-G100N and a combined mutant Mut-A69N-G100N show excellent relative enzyme activity, thermal stability and substrate tolerance. The mutant has high catalytic activity and stability to various chiral alcohols, and the contradiction that the traditional carbonyl reductase is easy to inactivate at high temperature and the catalytic efficiency is difficult to achieve at the same time is solved. The invention provides an efficient and stable enzyme catalysis tool for green biological preparation of chiral drug intermediates.

The invention relates to the technical field of nylon 6, and discloses a carbon nanotube grafted ABS modified nylon 6 composite material, acylating chlorinated carbon nanotubes and lysine are subjected to an amidation reaction, double bonds on ABS and double bonds on maleic anhydride are combined, anhydride modified ABS and amino modified carbon nanotubes are subjected to a ring-opening reaction, and the carbon nanotube grafted ABS modified nylon 6 composite material is obtained. The carbon nanotubes are grafted to the ABS, agglomeration of the carbon nanotubes is avoided, the carbon nanotubes can form a uniform network structure under the action of external force, a large amount of external force is absorbed, the toughness of a matrix is improved, a large amount of carboxyl introduced to the surface of the ABS plastic and amide groups in the nylon 6 matrix are subjected to hydrogen-bond interaction, a three-dimensional hydrogen-bond network is formed, and the toughness of the matrix is improved. According to the present invention, the compatibility and the interfacial force between the nylon 6 and the carbon nano-tube grafted ABS are improved, such that the nylon 6 and the carbon nano-tube grafted ABS can be uniformly distributed so as to form the sea island structure, and can buffer more stress effects under the action of the external force, such that the obtained composite material has excellent toughness.

The invention provides a recombinant collagen ionic liquid composition as well as a preparation method and application thereof, and relates to the technical field of cosmetics. The recombinant collagen ionic liquid composition provided by the invention is prepared from the following components in parts by mass: 19.8 to 79.99 parts of lysine citric acid ionic liquid, 20 to 80 parts of glycerol and 0.01 to 0.2 part of recombinant collagen, the lysine citric acid ionic liquid is mainly composed of lysine and citric acid, and the molar ratio of the lysine to the citric acid is (4-1): (2-1). The recombinant collagen ionic liquid composition is good in solubility, high in transdermal efficiency, small in irritation, short in preparation period, simple in process and capable of being continuously produced.

The invention discloses a ruminant feed capable of replacing soybean meal as well as a preparation method and application of the ruminant feed. The ruminant animal feed is prepared from the following raw material matrixes in parts by weight through compound probiotic fermentation: 45-55 parts of corn sugar residues, 15-25 parts of corn steep liquor, 10-20 parts of corn germ meal, 3-8 parts of extruded soybeans, 3-8 parts of corn protein powder, 2-4 parts of urea and 0.5-1.5 parts of lysine. The compound probiotics are prepared from saccharomyces cerevisiae, lactobacillus plantarum and bacillus subtilis. The invention further discloses a preparation method of the ruminant feed and application of the ruminant feed in completely replacing soybean meal in daily ration of ruminants. The ruminant feed disclosed by the invention can completely replace soybean meal in daily ration of ruminants, and even if the ruminants are in a fattening period, the soybean meal does not need to be additionally added to maintain protein supply, so that the feed cost is effectively reduced, and multiple targets of saving cost, improving efficiency and protecting environment are achieved.

The preparation method of the composite soybean protein gel comprises the following steps: adjusting the pH value of an egg white protein solution, carrying out heat preservation, adding protein glutaminase to carry out hydrolysis reaction, centrifuging, collecting supernate, and freeze-drying to obtain deamidated egg white protein; adding TG enzyme into the soybean protein isolate and deamidated egg white protein solution for enzymatic cross-linking reaction, centrifuging after enzyme deactivation, collecting supernate, and freeze-drying to obtain compound protein powder; dissolving the composite protein powder in water, stirring, and heating to form composite soybean protein gel; according to the preparation method disclosed by the invention, glutamine in the egg white protein is hydrolyzed by using the protein glutaminase, so that only the site of lysine is reserved, and then TG enzyme treatment is performed, so that the crosslinking of the egg white protein is avoided, and the content of disulfide bonds in the soybean protein is increased, thereby remarkably improving the gel strength and compression resistance of the composite soybean protein thermal gel.

The invention discloses functional caprolactam, an intrinsic flame-retardant nylon material as well as a preparation method and application thereof, and relates to the technical field of flame-retardant nylon materials. The preparation method comprises the following steps: preparing an alpha-amino-epsilon-caprolactam intermediate by taking lysine as an initial raw material through one-step efficient cyclization, then reacting with a reagent containing a flame-retardant group to obtain functional caprolactam, and finally introducing the flame-retardant group into a side chain of nylon 6 by utilizing ring-opening copolymerization of the functional caprolactam and caprolactam to prepare the intrinsic flame-retardant nylon material. The flame-retardant nylon material can effectively avoid the migration and loss of the flame retardant, improve the dispersity of the flame retardant and enhance the flame-retardant effect, can keep the main chain structure of the nylon 6 unchanged, reduces the influence on the inherent performance of the nylon 6, is expected to directly replace the existing nylon 6 material, and is applied to the fields of automobiles, textiles, electronics and electrics, buildings, packaging and the like. In addition, a renewable lysine resource is used for partially replacing a petroleum resource, so that the method has the advantages of resource conservation, environmental friendliness, simple process, low cost and the like.

The invention discloses a nutritional composition. The nutritional composition comprises vitamin D, basic protein and lysine, wherein in the nutritional composition, the mass ratio of the vitamin D to the basic protein to the lysine is (0.005 to 0.020) to (70 to 200) to (1000 to 3000). The invention also discloses an application of the nutritional composition and a formula food containing the nutritional composition. According to the nutritional composition disclosed by the invention, the vitamin D, the basic protein and the lysine can be matched with one another, so that linear growth of bones of children and teenagers can be effectively promoted, bone health and bone strength can be enhanced, and height development can be promoted.

The invention discloses a flame-retardant nylon material as well as a preparation method and application thereof, relates to the technical field of flame-retardant high polymer materials, and solves the problems that a flame-retardant nylon material prepared by a blending method is unstable in flame-retardant effect and a flame-retardant nylon material prepared by a copolymerization method is complex in process. The preparation method comprises the following steps: carrying out ring-opening copolymerization on a seven-membered ring lysine derivative monomer formed by ring formation of lysine and other lactam monomers to obtain copolyamide with a functional group on a side chain, and reacting a reagent containing a flame-retardant group with the functional group to prepare the flame-retardant nylon material. The flame-retardant nylon material prepared by the invention can effectively avoid migration and loss of the flame retardant, improve the dispersity of the flame retardant and enhance the flame-retardant effect. Meanwhile, a renewable lysine resource is used for partially replacing a petroleum resource, so that the method has the advantages of resource conservation, environmental friendliness, simple process, low production cost and the like. The flame-retardant nylon material disclosed by the invention has lasting and stable flame-retardant performance and can be widely applied to the fields of automobiles, textiles, electronics and electricity, buildings, packaging and the like.

The invention discloses a ganoderma lucidum culture solution and a culture method thereof. Comprising the following raw materials in parts: 30-40 parts of water, 10-20 parts of corn flour, 1-5 parts of ammonium nitrate, 1-5 parts of monopotassium phosphate, 1-5 parts of potassium sulfate, 1-5 parts of boric acid, 1-5 parts of manganese sulfate, 1-5 parts of zinc sulfate, 1-5 parts of copper sulfate, 1-5 parts of ferric sulfate, 1-5 parts of a short-acting growth regulator, 1-5 parts of amino acid, 1-5 parts of vitamins, 1-5 parts of a seaweed extract, 1-5 parts of honey and 1-5 parts of peaches and oranges. The short-acting growth regulator is prepared from gibberellin, auxin and cytokinin according to the ratio of (2-5): (1-8): (2-9), and the amino acid is prepared by mixing isoleucine, lysine, methionine and phenylalanine according to the ratio of (1-5): (2-8): (4-9): (5-7). The nutrient purity of the culture solution can be greatly increased, the fatality rate is reduced, cell elongation and rooting are promoted, plant metabolism and growth can be greatly promoted, and the quality of ganoderma lucidum is improved.

The invention discloses a high-efficiency lysine decarboxylase mutant and an application of the high-efficiency lysine decarboxylase mutant in synthesis of pentamethylene diamine. A series of mutants including a single mutant (such as N218G, T222V, C244P and the like), a double mutant and a three mutant are obtained by carrying out multi-site mutation on wild type lysine decarboxylase (SEQ ID NO.2) from Klebsiella grimontii. The catalytic efficiency of the mutant is remarkably improved, and the activity of the mutant is improved by 5.0-10.2 times compared with that of a wild type enzyme. According to the method disclosed by the invention, the substrate conversion rate of 99% or above can be realized within 2 hours through an optimized process of constructing an expression vector and a genetically engineered bacterium, inducing expression of lysine decarboxylase and synthesizing 1, 5-pentanediamine by utilizing a whole-cell catalysis technology. Compared with a traditional chemical method and a natural enzyme method, the method disclosed by the invention has the advantages of simplified process, high catalytic efficiency, low production cost and the like, and is suitable for producing bio-based 1, 5-pentamethylene diamine and nylon 56 salt.

The invention discloses an anti-scale cross-linked polymer fracturing fluid and a preparation method thereof, and relates to the technical field of petroleum fracturing fluids. When the anti-scale cross-linked polymer fracturing fluid is prepared, allylamine hydrochloride and epoxy chloropropane react to prepare a double-bond chlorinated intermediate; reacting the double-bond chlorinated intermediate with lauramidopropyl dimethylamine to prepare a cationic monomer; copolymerizing a cationic monomer and acrylamide to prepare a cationic polymer; the preparation method comprises the following steps: reacting L-lysine with formaldehyde and phosphorous acid to prepare phosphoric acid modified lysine; reacting the phosphoric acid modified lysine with allyl glycidyl ether to prepare a phosphoric acid modified monomer; acrylamide and an anionic monomer are copolymerized to prepare an anionic polymer; the anionic polymer and the cationic polymer are dissolved in water to prepare the anti-scale cross-linked polymer fracturing fluid. The anti-scale cross-linked polymer fracturing fluid prepared by the invention has the advantages of scale prevention, good temperature resistance, good shear resistance and self-crosslinking.

The invention belongs to the field of skin care products, and particularly relates to an anti-wrinkle and anti-aging composition as well as a preparation method and application thereof, and the anti-wrinkle and anti-aging composition comprises neurotransmitter inhibition peptide, signal peptide, madecassoside, inositol and glycosylglycerol. The neurotransmitter inhibition type peptide is at least three of acetyl hexapeptide-8, arginine / lysine polypeptide, dipeptide diaminobutyrylbenzylamide diacetate and acetyl octapeptide-3, and the signal type peptide is at least two of palmitoyl pentapeptide-4, decapeptide-4 and acetyl tetrapeptide-9. Compared with the prior art, all the components of the anti-wrinkle and anti-aging composition act on different anti-aging pathways and can be matched with one another from multiple aspects of oxidation resistance, inflammation resistance, moisturizing, cell viability enhancement, wrinkle reduction and the like, a more comprehensive and better anti-aging effect is provided, skin aging is comprehensively resisted, the skin is in a more young state, and the anti-wrinkle and anti-aging composition has the advantages that the anti-wrinkle and anti-aging effects are achieved. And the anti-wrinkle and anti-aging composition is small in irritation and good in stability.

The invention belongs to the technical field of biological medicine, and discloses an engineering bionic exosome with an anti-aging effect and a preparation method and application thereof, and the preparation method comprises the following steps: (S1) preparing a bionic cell membrane modified targeting ligand: adding collagen, mitochondrial targeting polypeptide, a condensing agent and 4-dimethylaminopyridine into an organic solvent, and stirring to obtain the bionic cell membrane modified targeting ligand; activating under a protective atmosphere, and then adding lysine into the system to react, so as to obtain a bionic cell membrane modified targeting ligand; and (S2) embedding the bionic cell membrane modified targeting ligand into the engineered exosome bionic membrane, and entrapping an active substance to form the engineered bionic exosome. The exosome provided by the invention has better skin permeability, mitochondrial targeting effect and cell endocytosis effect, has better protection effect on mitochondria, and can realize efficient delivery of active substances.

The invention discloses a full D-type antibacterial peptide with high enzymolysis stability and broad-spectrum antibacterial activity and application thereof. The antibacterial peptide is obtained by sequentially and repeatedly arranging D-type tryptophan, D-type arginine and D-type lysine for four times and carrying out C-terminal amidation; the amino acid sequence of the gene is (D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-NH2, and is marked as DWRK-12. The antibacterial peptide shows broad-spectrum antibacterial activity and excellent stability, can effectively resist clinically separated multidrug resistant strains, and keeps high cell selectivity at the same time. Due to the characteristics, the antibacterial peptide has important application value in the aspect of developing novel antibacterial drugs, especially in the field of treatment of infection of multiple drug-resistant bacteria.

The invention belongs to the field of biological medicine, and particularly relates to a respiratory syncytial virus F protein mutant and application thereof. The respiratory syncytial virus F protein mutant is characterized in that at least one pair of amino acid residues in an F1 subunit and / or an F2 subunit of F protein polypeptide are substituted by cysteine, and / or an F protein signal peptide part is substituted by a human immunoglobulin light chain signal peptide, and / or alanine A at the 107 site of a multi-base sequence is mutated into arginine R, lysine K at the 134 site is mutated into arginine R, and the amino acid residues in the F1 subunit and / or the F2 subunit of the F protein polypeptide are mutated into arginine. According to the invention, more neutralizing antibody epitopes can be exposed, the stable pre-fusion conformation is maintained, effective neutralizing antibody reaction and antibody binding reaction to the RSV subtypes A and B can be ensured, and the vaccine is suitable for various vaccine forms taking the RSV F protein as the antigen.

The invention discloses a macromolecular antibacterial agent, an antibacterial nylon material as well as a preparation method and application thereof, and relates to the technical field of antibacterial macromolecular materials. According to the invention, lysine is used as a raw material, the polymer antibacterial agent is prepared through reactions such as dehydration ring formation and re-ring opening polymerization, the polymer antibacterial agent has the characteristics of high safety, biodegradability and good biocompatibility, and has the same polyamide main chain structure as nylon, the polymer antibacterial agent and nylon have good compatibility, and the polymer antibacterial agent and nylon 6 are fused and blended to form a nylon 6 sample. Through amide exchange reaction and hydrogen bond interaction between the macromolecular antibacterial agent and nylon 6 molecular chains, the antibacterial nylon material with excellent mechanical property and lasting and stable antibacterial effect is prepared, and the requirements of people for safety and environmental friendliness are met. The prepared antibacterial nylon material can be applied to antibacterial products such as textiles, daily necessities and packaging materials. Meanwhile, the macromolecular antibacterial agent and the antibacterial nylon material are simple in preparation process, low in raw material cost and suitable for industrial production.

The invention relates to nanoparticle-loaded antibacterial composite hydrogel as well as a preparation method and application thereof, a hydrogel network main body is formed by grafting phenylboronic acid to methacrylated I-type collagen rCol1MA-PBA and methacrylated polylysine EPLMA and adding glucose oxidase GoX for crosslinking, and is loaded with core-shell nano-enzyme C (at) Z (at) T at the same time, and the core-shell nano-enzyme C (at) Z (at) T is loaded with the core-shell nano-enzyme C (at) Z (at) T and the core-shell nano-enzyme C (at) Z (at) T; the core-shell nano-enzyme C (at) Z (at) T is composed of a spherical CeO2 nano-particle core, a zeolite imidazate framework material TIF-8 shell and tannic acid TA coating the shell. The raw materials of the hydrogel are easy to obtain, the preparation method is simple, the price is low, and the components are safe and reliable; in addition, multiple functions of resisting bacteria, resisting inflammation, resisting oxidation, promoting angiogenesis and the like are integrated, a more comprehensive treatment effect is provided, and the dressing can be used for healing the wound surface of diabetes mellitus.

The invention relates to the field of polypeptide compositions, in particular to a polypeptide metal chelate and application thereof in the field of cosmetics, and the polypeptide metal chelate is formed by chelating a polypeptide compound and a metal ion compound. The structure of the polypeptide compound is [gamma-Glu] n-AA, n is greater than or equal to 1, and AA is selected from glycine, alanine, valine, leucine, isoleucine, methionine (methionine), proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, asparaginic acid, glutamic acid, lysine, arginine or histidine; the polypeptide chelated zinc prepared by the invention has remarkable oil control and anti-inflammatory effects, and can be compounded with other raw materials to form cosmetics with oil control and acne removal effects.

The invention discloses a hybrid sturgeon feed and a preparation method thereof, and relates to the technical field of sturgeon feeds, and the key point of the technical scheme is that methionine and lysine are supplemented in a feed in which soybean protein concentrate replaces fish meal, so that the influence of methionine and lysine on the growth performance, liver and intestinal health and muscle quality of hybrid sturgeons is improved. The soybean protein concentrate is used for replacing the fish meal, so that the dosage and production cost of the fish meal are greatly reduced, and meanwhile, the methionine and the amino acid are added, so that the negative influence caused by replacing the fish meal with the soybean protein concentrate is avoided.

The invention discloses a cyclic nonapeptide with repairing and anti-oxidation effects and application of the cyclic nonapeptide, the amino acid sequence of the cyclic nonapeptide is cyclic (arginine-glycine-aspartic acid-serine-arginine-lysine-valine-lysine-serine), the prepared cyclic nonapeptide has the repairing and anti-oxidation effects, and the cyclic nonapeptide has the repairing and anti-oxidation effects. The composition can be applied to preparation of cosmetics with repairing and / or anti-oxidation effects.

The invention provides a plant lactobacillus TH13, and the preservation number of the plant lactobacillus TH13 is CGMCC (China General Microbiological Culture Collection Center) NO.32112. The plant lactobacillus TH13 provided by the invention can be used for fermenting folium cortex eucommiae and enzymatically hydrolyzed bone meal, and the fermentation product of the plant lactobacillus TH13 has high content of chlorogenic acid, proline, glycine and lysine. The fermentation product can significantly improve the content of alkaline phosphatase (ALP) and osteocalcin (OCN) in mouse serum and reduce the content of tartrate-resistant acid phosphatase (TRACP). Therefore, the fermentation product can significantly improve the bone mineral density of the femur, increase the number of bone trabecula and significantly reduce the separation degree of the bone trabecula, thereby promoting bone formation of a mouse with senile osteoporosis, inhibiting bone resorption and improving senile bone loss and bone microstructure deterioration. The preparation method of the probiotic preparation containing the plant lactobacillus and the probiotic fermentation product is simple, and large-scale production can be realized.

The invention relates to a multi-effect bionic liposome transdermal repair carrier and a preparation method and application thereof.The multi-effect bionic liposome transdermal repair carrier comprises active ingredients and a nano-carrier, and the active ingredients comprise sialic acid, saccharomycetes / rice fermentation product filtrate, arginine / lysine polypeptide and vitamin E in the mass ratio of (1-10): (2-8): (0.1-1): (0.5-4); the nano-carrier is prepared from the following raw materials: phospholipid, an emulsifier, polyhydric alcohol and water. Compared with the prior art, the anti-aging, whitening and moisturizing composition has the advantages of enhancing skin moisturizing and anti-oxidation effects, promoting collagen synthesis and inhibiting tyrosinase to achieve remarkable anti-aging, whitening and moisturizing effects and the like.

The invention provides a recombinant humanized elastin with high biological activity as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering and biological materials. 100% of the amino acid sequence of the elastin is derived from human elastin and is prepared by expression of genetically engineered bacteria; the protein has a typical beta-spiral hydrophobic structural domain and a lysine-rich hydrophilic structural domain, has a good bionic structure and functional characteristics, can significantly promote proliferation, adhesion and migration of human skin fibroblasts, also has excellent antioxidant, anti-wrinkle and anti-aging activities, and can be used for preparing a biomimetic protein. The hydrogel can be widely applied to the fields of skin repair dressings, injection implants, artificial skin, biomedical materials, functional skin care products and the like; in addition, the elastin is good in solubility, high in stability, mature in expression system and suitable for large-scale production.

The invention relates to the field of oncology and diagnosis, in particular to a target spot for treating pancreatic cancer and application of the target spot. Experiments discover that the 176-site lysine lactylation modification (HNRNPC K176la) level of the HNRNPC protein is remarkably increased in pancreatic cancer, existence of site modification is confirmed through a pan-lactylation modification antibody for the first time, and meanwhile, the K176 site is confirmed to be a key site for carrying out lactylation modification on the HNRNPC in the pancreatic cancer. Furthermore, in-vivo and in-vitro functional experiments prove that HNRNPC K176 lactic acid modification can inhibit proliferation of pancreatic cancer cells and increase of tumor volume.