9 results about "Malic enzyme" patented technology

The invention belongs to the technical field of biology, and discloses an exogenous cofactor enhanced yarrowia lipolytica strain, a method and application in efficient synthesis of EPA (eicosapentaenoic acid) and lipids.The chassis strain of the yarrowia lipolytica strain is yarrowia lipolytica Po1f, desaturase is partially prolonged in an overexpression fatty acid synthesis route in the strain, and the EPA and the lipids.The yield of the EPA and the lipids.The yield of the EPA and the lipids.The yield of the EPA and the lipids. The desaturase gene mainly comprises a delta 9 elongase gene, a delta 8 desaturase gene, a delta 5 desaturase gene and a delta 17 desaturase gene, and G6PDH and malic enzyme are overexpressed at the same time on the basis. The strain provided by the invention improves the EPA and lipid yield of yarrowia lipolytica; the method is suitable for constructing a genetic engineering strain with high yield of EPA and lipid, provides a basis for producing grease rich in EPA from yarrowia lipolytica, and solves the problems of non-sustainability, high cost and the like caused by obtaining EPA from fish.

The invention discloses a modified aureobasidium pullulans strain based on reduction of malic acid pathway consumption and application of the modified aureobasidium pullulans strain. Gene for coding NADPH dependent malic enzyme and phosphoenolpyruvate carboxykinase in an aureobasidium pullulans genome is directionally knocked out. The transformation blocks a key consumption branch for synthesis and accumulation of the cytoplasmic malic acid, and effectively guides a central carbon metabolic flow to be more efficiently guided to a target product. Experimental results show that the polymalic acid yield and the saccharic acid conversion rate of the double-knockout engineering strain D3N5-delta3617 / deltapepck in fermentation are remarkably improved compared with those of an original strain, and the biomass is also increased. The invention provides a high-performance strain with important application value for industrial efficient fermentation production of polymalic acid.

The invention relates to the technical field of enzyme engineering, in particular to a malic enzyme mutant and application thereof. Specifically, the malic enzyme derived from Escherichia coli is subjected to site mutation screening, and results show that after methionine at the 144th site is mutated into isoleucine (M144I), glutamine at the 253rd site is mutated into isoleucine (Q253I), and threonine at the 327th site is mutated into phenylalanine (T327F), the catalytic efficiency and the catalytic activity are obviously improved, which is of great significance to industrial application.

The invention discloses application of ME1 in enhancing the anti-tumor function of CAR-T cells, and relates to a preparation method of a chimeric antigen receptor plasmid structure of overexpressed malic enzyme 1 for enhancing the killing activity of the CAR-T cells, in particular to the malic enzyme 1 serving as a gene for enhancing the functions of the CAR-T cells. When the compound is combined with a CAR-T cell treatment product for application, the anti-tumor effect can be remarkably improved.

The invention relates to the technical field of enzyme engineering, in particular to a malic enzyme mutant and application thereof. Specifically, the malic enzyme derived from Escherichia coli is subjected to site mutation screening, and the result shows that after valine at the 7th site is mutated into lysine (V7K), the catalytic efficiency and the catalytic activity are obviously improved, which is of great significance to industrial application.

Methods and materials for increasing expression of malic enzyme 1 (ME1) in T cells are provided herein, as are compositions containing ME1 coding sequences or ME1 mRNA, and methods and materials for using T cells with increased ME1 levels to treat mammals having cancer.

The invention discloses a high-yield R-HPPA recombinant beauveria bassiana engineering bacterium, a construction method and application, an NADPH metabolic pathway is enhanced based on a multi-gene synergistic overexpression strategy, and efficient dynamic coupling of cofactor NADPH supply and hydroxylation reaction is realized by systematically regulating and controlling a phosphopentose pathway and a malic enzyme pathway, so that the yield of R-HPPA is increased, and the yield of R-HPPA is increased. According to the present invention, the beauveria bassiana hydroxylation reaction efficiency is improved, the efficient biosynthesis of the R-2-(4-hydroxyphenoxy) propionic acid is achieved, the fermentation process is optimized so as to promote the industrial application, and the new path is provided for breaking through the industrial technology bottleneck of the R-2-(4-hydroxyphenoxy) propionic acid biosynthesis.

The application belongs to the technical field of biology, and discloses an exogenous cofactor enhanced Yarrowia lipolytica strain, a method and application in high-efficiency synthesis of EPA and lipids. The chassis strain of the Yarrowia lipolytica strain is Yarrowia lipolytica Po1f, and part of elongated desaturase in a fatty acid synthesis pathway, mainly including Δ9 elongase, Δ8 desaturase, Δ5 desaturase and Δ17 desaturase genes, is overexpressed in the strain. On this basis, G6PDH and malic enzyme are simultaneously overexpressed. The strain improves the EPA and lipid yield of Yarrowia lipolytica. The application is suitable for constructing a gene engineering strain with high EPA and lipid yield, and provides a basis for Yarrowia lipolytica to produce EPA-rich oil, so as to solve the problems of unsustainability and high cost caused by obtaining EPA from fish.

The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel NADP-dependent malic enzyme variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The NADP-dependent malic enzyme variant according to the present invention is obtained by substituting one or more amino acids in the amino acid sequence constituting NADP-dependent malic enzyme to change the enzymatic activity of the NADP-dependent malic enzyme, and a recombinant microorganism comprising the NADP-dependent malic enzyme variant is capable of efficiently producing L-glutamic acid.