89 results about "Hexose" patented technology

The present disclosure relates to processes for producing hydrocarbon fuels from lignocellulosic biomass. A process may include introducing biomass to a pretreatment system, and a first separation system forming a pentose-rich stream and a pentose-lean stream. The pentose-lean stream may be introduced to a hydrolysis system forming a hydrolysate and the hydrolysate introduced to a second separation system forming a hexose-rich stream and a lignin stream. Additionally, at least one of the pentose-rich stream or the hexose-rich stream may be introduced to a bioreactor containing microorganisms configured to produce hydrocarbon fuels. Additionally, the present disclosure also relates to systems for the production of hydrocarbon fuels. A system may include a pretreatment system, a first separation system, a hydrolysis system, a second separation system, and one or more bioreactors. Alternatively a system may include a pretreatment system, a hydrolysis system, a sugar separation system, and one or more bioreactors.

The invention provides an application of a peach transport protein PpSWEET11 in regulating and controlling the hexose content of fruits. According to the invention, the function of the PpSWEET11 gene is cloned and verified, and through overexpression of the PpSWEET11 in peach fruits and tomatoes, the glucose and fructose levels in an overexpression group are obviously increased compared with those in a control group, which indicates that the PpSWEET11 has a wide sugar transport function. The invention not only expounds the effect of the PpSWEET11 in the aspect of sugar accumulation and provides a key molecular insight in the aspect of improving sugar accumulation of peaches and other economically important fruit crops, but also can be applied to nutritional quality improvement and germplasm innovation of peach fruits.

The invention discloses an alpha-glucan phosphorylase (alpha GP) mutant. The amino acid sequence of the alpha-glucan phosphorylase mutant is as shown in SEQ ID NO. 3. The invention also discloses an application of the mutant in enzymatic synthesis of hexose, especially mannose. The alpha-glucan phosphorylase mutant provided by the invention has higher catalytic activity, can significantly improve the production efficiency of mannose, and can be used for industrial production of mannose.

The present invention relates to a non-invasive test for the detection of deep endometriosis. Particularly, although not exclusively, aspects of the present invention relate to one or more glycolysis-associated biomarkers (e.g., phosphoglycerate mutase 1 (PGAM1), Enolase-1 (ENO1), phosphofructokinase-1 (PFKP) and / or Hexokinase) and / or cytokines associated with aerobic glycolysis (e.g., IL-6 and / or TGF-β) which can be used to determine the likelihood of a subject having deep endometriosis based on a sample obtained from the subject.

The present invention provides immunogenic compositions and vaccines comprising isolated gonococcal outer membrane vesicles (OMVs). Said isolated gonococcal OMVs display lipooligosaccharide (LOS) glycan structures comprising an oligosaccharide alpha-chain elongating from Hep I having at least four hexose monosaccharides (4Hex or 5Hex) and wherein said LOS is detoxified. The present invention relates to the use of said immunogenic compositions and vaccines in medicine and, more particularly, the use of said immunogenic compositions and vaccines in immunizing a subject against Neisseria gonorrhoeae infection.

This invention provides a method for the co-production of organic acids and lignin from lignocellulose, comprising the following steps: Step 1, pretreating the lignocellulose raw material to obtain a pretreated material; Step 2, enzymatically hydrolyzing the pretreated material to obtain an enzymatic hydrolysate containing pentoses, hexoses, and lignin; Step 3, performing solid-liquid separation on the enzymatic hydrolysate to obtain an enzymatic hydrolysate containing pentoses and hexoses and a solid phase containing lignin; Step 4, subjecting the enzymatic hydrolysate containing pentoses and hexoses to acid-producing fermentation to obtain a fermentation broth containing a mixture of organic acids; Step 5, extracting lignin from the lignin-containing solid phase to obtain lignin. The method provided by this invention can efficiently separate lignin and obtain various organic acids through a simple process.

The present application relates to starch production technical field, especially disclose a kind of green preparation method for modified starch for replacing hydroxypropyl distarch phosphate.The green preparation method, cassava starch is used as raw material, specifically includes the following steps: (1) cassava starch is mixed with water uniformly, and starch suspension is prepared;(2) adding alpha-amylase to starch suspension for glycosidic bond enzymolysis, then passivate enzyme activity;(3) the enzymatic hydrolysate is used to hexose oxidase and oligomeric glucan is subjected to lactonization reaction, then passivate enzyme activity;(4) the final enzymatic hydrolysate is centrifuged, washed with water, the precipitate is freeze-dried, and enzyme modified cassava starch is obtained.The paste viscosity, freeze-thaw stability, shear resistance, anti-aging and other chemical properties of the enzyme modified cassava starch prepared by the present application are close to or better than hydroxypropyl distarch phosphate, and the preparation process is simple, short cycle, green and safe, low in production cost, simple in equipment, suitable for industrial production, and safe and pollution-free during production.

The present disclosure provides genetically engineered microorganisms for the simultaneous fermentation of pentose and hexose sugars, for example, glucose and xylose. The microorganisms can be modified to express AtSWEET polypeptides, LST1 polypeptides, mutants thereof, homologs thereof or combinations thereof. Also provided are methods of co-fermenting hexose and pentose sugars, methods of increasing the conversion of lignocellulosic biomass via microbial fermentation, and methods of generating biofuel.

The invention discloses an indole pyridine chalcone compound and application thereof in medicine for treating colitis, and belongs to the technical field of biological medicine. The compound has a structure as shown in a formula I, wherein R1 is selected from C1-C8 alkyl, C1-C8 alkoxy, halogen, cyano, nitryl and morpholinyl, and R2 is selected from nitryl. According to the invention, multiple excellent pharmacophores such as indole, pyridine, chalcone, sulfonamide and the like are fused in the same molecule for the first time, the preparation method does not need pre-activation of a substrate, does not need a metal catalyst or an additive, and has the advantages of mild reaction conditions, high yield, good selectivity, low cost and environmental friendliness. The compound has strong inhibitory activity on hexokinase 2 (HK2), can significantly inhibit inflammatory response and regulate macrophage polarization, shows an excellent colitis treatment effect in in-vivo and in-vitro experiments, provides a new choice for developing novel colitis treatment drugs, and has a wide medical application prospect.

To provide an immobilized enzyme composition for hexose production or an improved process for hexose production. [Solution] The present invention relates to an immobilized enzyme composition for the preparation of hexoses. Examples of hexoses include tagatose, psicose, fructose, allose, mannose, galactose, altrose, talose, sorbose, gross, idose, and inositol. The present invention also relates to an enzymatic process for preparing hexoses from sugars by contacting starch derivatives with the immobilized enzyme composition of the present invention.

Disclosed is an isoform of hexokinase 1 (HK1), namely hexokinase 1b (HK1b), for use as a prognosis marker and as a specific target for use in treatment of cancer.

The application uses cheap and easily available hexose acid as a starting material, first obtains two precursors of 2,5-furan dicarboxylic acid synthesis, namely hexahydroxy acid and 4-deoxy-5-dehydro-hexahydroxy acid through enzyme catalytic reaction, and then 4-deoxy-5-dehydro-hexahydroxy acid is dehydrated under acidic conditions to obtain 2,5-furan dicarboxylic acid.

The invention belongs to the technical field of plant genetic engineering, and relates to an application of a Xanthomonas oryzae hexose phosphate mutase encoding gene in the aspect of adjusting the susceptibility of cruciferous plant arabidopsis thaliana. According to the present invention, the recombinant plasmid is constructed, the Xanthomonas oryzae pv.oryzae (Xoo) PXO99A hexose phosphate mutase coding gene PXO03174 is adopted as the target gene to be introduced into the cruciferous plant arabidopsis thaliana, such that the condition that the plant with the overexpression of the gene is sensitive to the infection of the pathogenic bacterium Pseudomonas syringae pv.tomato (Pst) DC3000 is found, and the significantly enhanced disease infection characteristic is represented; according to the present invention, the discovery can be used for developing the sensitive bacterial disease detection method so as to improve the accuracy of bacterial disease detection; the method can also be used for identifying toxic genes of plant pathogenic bacteria such as pathogenic bacteria sensitive plants and pseudomonas syringae and researching the mechanism of the infected plants, and can also be used for researching the disease resistance of the plants to the pseudomonas syringae and developing antibacterial agents.

The present invention provides a hexokinase, a preparation method thereof and an application. Among them, the hexokinase is obtained by mutating the hexokinase gene of wild-type Saccharomyces cerevisiae; the 52nd position of the amino acid sequence of the hexokinase is replaced and modified to E52V, and the 254th position is replaced and modified to S254P; the amino acid sequence is as shown in SEQ ID N0.1. The technical problem solved by the present invention is that the cost of the kit is high and the stability is poor during the detection of glucose by the hexokinase method.

This invention provides compositions for treating diseases, disorders, and symptoms related to ketohexokinase (KHK) activity. [Solution] The present invention provides RNAi agents, such as dsRNA agents, that target the ketohexokinase (KHK) gene. The present invention also provides a method of using RNAi agents to inhibit KHK gene expression in a target, and a method for treating or preventing KHK-related diseases.

A drug conjugate, composition, and method for delivering active antimicrobials based on existing antibiotics through a hexose phosphate transporter (UhpT) by conjugating the antimicrobials with non-metabolizable hexose phosphates. Methods of co-administering antibiotics with non-metabolizable hexose phosphates as antimicrobials are also disclosed. Non-metabolizable hexose phosphates can constitutively and strongly induce expression of UhpT which significantly improves the efficacy and / or antimicrobial spectrum of antibiotics. This drug conjugate, composition and method will permit reuse of many FDA approved antibiotics that have been abandoned or fallen into disuse due to their current low efficacy and / or resistance to these antibiotics by pathogens.

The invention belongs to the technical field of Baijiu brewing, and particularly relates to a method for directionally enhancing the flavor of high-temperature yeast for making hard liquor. In order to break through the bottlenecks of single substrate utilization and uncontrollable reaction path in the existing yeast flavor regulation and control technology, the yeast flavor regulation and control method disclosed by the invention comprises the following steps: accurately proportioning pentose, hexose, disaccharide, sulfur-containing amino acid and aromatic amino acid, pretreating, preparing a solution, treating yeast-making raw materials, accurately controlling the temperature in the whole fermentation process, turning over yeast and the like. The Maillard reaction path in the high-temperature yeast preparation process is directionally regulated and controlled. Experiments show that the content of pyrazine and terpene compounds in the high-temperature Daqu can be remarkably increased, the characteristic flavors such as wood fragrance, baking fragrance and scorch fragrance are synchronously enhanced, and a systematic technical scheme is provided for regulating and controlling the flavor quality of Baijiu.

The invention discloses a novel medical application of low-medium glycans in American ginseng and a medicinal preparation of the low-medium glycans in American ginseng. The invention provides low-medium glycans separated from American ginseng. The low-medium glycans are mainly formed by polymerizing 2-15 hexose molecules. The hexose molecule is composed of glucose and fructose. The American ginseng low-medium glycan component provided by the invention not only can down-regulate the expression level of PD-L1 in tumor cells, but also can reduce the expression level of PD-1 in immune cells such as T cells, effectively activate the T cells and hinder tumor immune escape, has the application of preparing a PD-L1 inhibitor, a PD-1 inhibitor, a PD-L1 and PD-1 dual inhibitor or an anti-tumor drug, and can be used for preparing the anti-tumor drug. The compound can be used for immunotherapy of various cancers such as lung cancer, breast cancer, colon cancer, melanoma and the like, and has an application prospect in the aspect of tumor immunotherapy.

Disclosed herein are methods of producing hexoses from saccharides by enzymatic processes. The methods utilize fructose 6-phosphate and at least one enzymatic step to convert it to a hexose.

The present invention aims to provide a novel sugar compound. 【Solution means】A sugar compound or a salt thereof containing a chemical structure derived from an amino acid and a chemical structure derived from a sugar, wherein the chemical structure derived from the amino acid is a 2-amino-3-phenylpropyl group or the like, and the chemical structure derived from the sugar contains a repeating unit derived from one or more hexoses and / or a repeating unit derived from one or more uronic acids, the hexose being glucose or the like; the uronic acid being glucuronic acid, a sugar compound or a salt thereof (however, the sugar compound or a salt thereof excludes compound (A): a sugar compound or a salt thereof in which the chemical structure derived from the amino acid is a phenylalanine residue, a 2-amino-3-phenylpropyl group, or a tyrosine residue, and the chemical structure derived from the sugar is only the repeating unit derived from the hexose).

The application relates to a stone moss polysaccharide cleavage enzyme JpPL40A and application thereof, and belongs to the technical field of functional enzymes. The amino acid sequence of the stone moss polysaccharide cleavage enzyme JpPL40A is shown as SEQ ID NO. 1, and the coding gene is shown as SEQ ID NO. 2 or SEQ ID NO. 3. The stone moss polysaccharide cleavage enzyme JpPL40A can hydrolyze stone moss to obtain unsaturated stone moss disaccharide, tetrasaccharide, hexose and / or octose.

This invention relates to a process, a plant, and a biofuel for integrated biofuel production, such as with butanol, biodiesel, and / or sugar product. The integrated process includes the step of removing hexose from a feedstock to form a lignocellulosic material. The process also includes the step of converting the hexose to butanol and / or a biodiesel material, and the step of depolymerizing lignocellulosic material to form pentose and a residue. The process also includes the step of converting the pentose to butanol and / or a biodiesel material.

The application provides application of biomarkers in preparation of products for diagnosing systemic lupus erythematosus, and belongs to the field of biological medicine. The biomarkers include C3_N85-HexNAc(2)Hex(1); C3 is complement C3, the amino acid sequence of which is shown as SED ID NO.3; N is an amino acid residue, and the number after N is a site; HexNAc is a hexosamine residue, Hex is a hexose residue, NeuAc is a sialic acid residue, and the number in the bracket represents the number of corresponding monosaccharide residues. The biomarkers also include at least one of F2_N121-HexNAc(2)Hex(12), VTN_N169-HexNAc(2)Hex(4) and APOB_N1523-HexNAc(3)Hex(4)NeuAc(1). The application proposes that the above protein N-glycopeptide is used as a biomarker for diagnosing SLE, has the advantages of high accuracy, high sensitivity and high specificity, and provides a new target for diagnosis and intervention improvement of SLE patients.

The invention belongs to the field of molecular biology, and particularly relates to application of an MdHT13.2 gene in regulation and control of plant growth. According to RNA-seq (Ribonucleic Acid-Seq) data analysis, MdHT13.2 with high expression quantity in apple leaves is screened out. Mutant yeast sugar absorption complementation experiments show that MdHT13.2 not only has transport activity on hexose such as glucose, fructose and the like, but also has stronger transport capacity on cane sugar. The results of in-situ hybridization of apple leaves and GUS staining experiments of arabidopsis show that MdHT13.2 has a high expression level in the phloem of the vein. The MdHT13.2 as a member in a hexose transporter gene family can regulate and control sugar accumulation in plants and promote growth of the plants.

The invention discloses a recombinant bacterium and an application of the recombinant bacterium in production of a tanshinone precursor 11, 20-dihydroxy ferruginol. The recombinant bacterium contains or expresses diterpenoid synthase KSL1 and TPS1, and galactokinase, difunctional UDP glucose 4-isomerase / aldose 1-isomerase and hexose-1-phosphate uridine transferase are knocked out or inhibited. According to the invention, de novo synthesis of 11, 20-dihydroxy ferruginol is realized through a plurality of strategies, and the yield of 11, 20-dihydroxy ferruginol is obviously improved. The recombinant strain provided by the invention can be used for efficiently producing 11, 20-dihydroxy ferruginol and other tanshinone precursors, and has an important application value.

The invention discloses an alpha-glucan phosphorylase (alpha GP) mutant. The amino acid sequence of the alpha-glucan phosphorylase mutant is as shown in SEQ ID NO. 3. The invention also discloses an application of the mutant in enzymatic synthesis of hexose, especially inositol. The alpha-glucan phosphorylase mutant provided by the invention has higher catalytic activity, and the yield of inositol can be remarkably increased and is far greater than the yield disclosed in the prior art.

The application discloses a kind of multistage series reaction and constant boiling rectification water removal coupling 2,5-furan dicarboxylic acid continuous preparation device and method.The continuous preparation device is by 2-6 gap reaction kettle of coupling constant boiling rectification water removal series connection composition.The steps of the method are as follows:1) single gap reaction kettle stable operation;2) multistage series reaction kettle stable continuous operation;3) continuously adding hexahydroxydicarboxylic acid and / or hexahydroxydicarboxylic acid salt to the first stage gap reaction kettle, reaction at 100~140 ℃;4) the reaction product from the last stage gap reaction kettle is obtained after cooling, crystallization, recrystallization 2,5-furan dicarboxylic acid product.The application realizes 2,5-furan dicarboxylic acid continuous production, substrate concentration can be increased to 30%, reaction selectivity is good, space-time yield is high, product uniformity is good, suitable for large-scale industrial application, with good industrial application prospect.

The invention discloses a metabolic antagonist solution which is obtained by dissolving a metabolic antagonist in water, the metabolic antagonist is L-2-enol hexolactone and / or 2-deoxy-D-glucose, and the invention also discloses an application of the metabolic antagonist solution in storage of crude rosin. The metabolism antagonist solution provided by the invention aims at glycolysis and energy metabolism of impurities such as pine needles and barks, and discoloration and degradation of the crude rosin in the storage process are blocked from the source; the L-2-enol hexanoic acid lactone oxidation product enters cells through glucose transporters such as GLUT1 / 3 / 4, competes for binding sites with glucose, and interferes with glycolysis and energy metabolism of impurities such as pine needles and barks; the 2-deoxy-D-glucose has a pyranoid ring conformation highly similar to that of glucose, is acidified into 2-deoxyglucose-6-phosphoric acid by hexokinase, and hijacks a glycolytic pathway, resulting in metabolic blocking and energy depletion.