The invention discloses a method for improving the purity of mogroside V in mogroside. Fresh siraitia grosvenorii or dried siraitia grosvenorii serves as raw materials. The method includes the steps of 1, extraction, 2, preconcentration, 3, enzymolysis, 4, primary filtering, 5, resin adsorption and elution, 6, concentration and secondary filtering, 7, resin column desalting, 8, resin column decoloring, 9, further activated carbon decoloring, and 10, sterilization and drying. An adsorption column is used for adsorbing main effective substances in feed liquid, eluant is used for eluting the effective substances on the adsorption column, finally, the foreign smell of the eluant is removed through desalting resin, pigment is removed through decoloring resin, impurities are further removed through activated carbon adsorption, and the product is obtained after sterilization and drying. The final product is snow white, free of foreign smells, fresh and sweet in taste, lasting in later sweetness, similar to white granulated sugar and capable of being applied to sugar substitute products and weight loss and healthcare food, the sweetness is 500-1000 times that of granulated sugar, and meanwhile the calory is almost 0.