The invention discloses a tree
peripheral bark callus inducer as well as a preparation method and a use method thereof, and belongs to the technical field of
plant tissue
hyperplasia. The
inducer is prepared by mixing the following components in parts by
mass: 900 to 1100 parts of water, 90 to 130 parts of a
humectant, 0.01 to 0.5 part of a proliferation agent and 0 to 15 parts of a protective agent. When the
inducer is prepared, firstly, water is heated to 50-60 DEG C, under the condition of heat preservation, the
humectant weighed according to the proportion is added and fully stirred to be dissolved, then the protective agent weighed according to the proportion is added and fully stirred to form a homogeneous
system, then the temperature is reduced to the
room temperature, the inducer weighed according to the proportion is added, and the homogeneous
system is formed after the inducer is fully stirred again. When in use, the inducer is prepared according to the formula proportion and the method; the composition is sprayed on a fresh
wound surface of a peeled
bark, the dosage is 0.06-0.10 ml / cm < 2 >, and then the
wound surface is wrapped by a degradable film. The inducer can effectively shorten the
callus time of peridermis, promote the formation of hyperplastic tissues, increase the growth amount of
cork and improve the quality uniformity of the
cork.