The invention discloses a method for simultaneously determining multiple wooden plug pollutants in grape wine, and belongs to the technical field of grape wine detection. The method mainly comprises the following steps: derivatization treatment of a target object and an internal standard, determination of characteristic ions of the target object and the internal standard, establishment of a gas chromatography-tandem mass spectrometry selective reaction monitoring method, determination of a standard curve and detection of the cork pollutants in the grape wine. The method can be used for simultaneously determining nine wooden plug pollutants such as 2, 4, 6-trichloroanisole, 2, 3, 4, 5-tetrachloroanisole, 2, 3, 4, 6-tetrachloroanisole, pentachloroanisole, 2, 4, 6-trichlorophenol, 2, 3, 4, 6-tetrachlorophenol, pentachlorophenol, 2, 4, 6-tribromoanisole and 2, 4, 6-tribromophenol. The detection method is good in linear correlation and standard recovery rate, the detection limit is 0.1 ng / L-0. 5 ng / L, and a technical guarantee can be provided for detection of the cork pollutants in the wine.