The invention discloses a construction method and application of an
aspongopus characteristic
peptide spectrum, and the method comprises the following steps: preparing an
aspongopus test sample solution and an
aspongopus control medicinal material reference substance solution, analyzing by an ultra-
high performance liquid chromatography-
mass spectrometer, and determining the following six characteristic
ion peaks with double-charge parent ions from
a peptide spectrogram of the
test sample, the characteristic
peptide spectrum of the aspongopus is as follows: m / z is 469.263 to 522.312, 617.811 to 731.402, 559.780 to 760.400, 649.800 to 785.350, 623.840 to 430.260, and 789.926 to 1167.596. The characteristic
peptide spectrum of the aspongopus can be used for preparing the aspongopus. According to the method, true and false identification of the aspongopus medicinal material, the standard decoction and the formula granules can be simultaneously realized, and adulterants such as
jatropha curcas,
lysimachia plicata and amblycoris nuda can be effectively distinguished. The method is high in specificity, good in reproducibility, easy and convenient to operate and high in detection efficiency, the technical problem that a traditional identification method is not suitable for a
water extraction preparation is solved, and reliable guarantee is provided for
quality control of the aspongopus formula granules.