133 results about "Jatropha" patented technology

The present invention provides methods for increasing oil content in Jatropha Curcas L. plants. The invention further provides the cDNA and protein sequences of Jatropha acetyl CoA carboxylase (ACCase) and methods for cloning and expressing the Jatropha ACCase gene to produce transgenic plants with increased oil content.

The invention relates to the bio-polyol prepared by the Jatropha Curcasl oil by the following methods: the Jatropha Curcasl oil is added with the epoxidizing agent to conduct epoxidation reaction to produce epoxy Jatropha Curcasl oil under the condition that the catalyst exists; the produced epoxy Jatropha Curcasl oil conducts the epoxy bond addition reaction with the ring opening agent containing active hydrogen to produce the mixed hydroxyl fatty acid glyceride; after that, the alcohol or the alcohol amine are added and heated to carry out the alcoholysisreaction to produce the mixed hydroxyl fatty acid ester, namely the bio-polyol. The invention has the advantages that the inventions is high in reaction degree, wide in operation process range, convenient in control, higher in production functionality, convenient in raw material purchase and lower in cost, and does not fight for food with the people and is not affected by the petroleum resource, which is a green product optimizing the ecology. In addition, the invention also has the advantages of strong technology collectiveness and high product conversion.

The present invention relates to methods of reducing chemical fertilizer usage and greenhouse gas nitrous oxide emission and to methods of improving plant growth rate and seed productivity in agriculture through the application of a novel artificially manufactured formula containing a nitrogen-fixing bacterium that efficiently colonizes non-legume plants in aerial parts and the root system. The bacteria inocula and methods are particularly suitable for plants in the genera Jatropha, Sorghum, Gossypium, Elaeis, Ricinus, Oryza and Manihot.

The invention discloses a special growth regulating agent for jatropha and application thereof. The growth regulating agent is prepared according to the following steps: dissolving cytokinin into a sodium hydroxide solution; then, using distilled water for diluting the solution into mother liquid with the cytokinin concentration between 0.02 mg / mL and 100 mg / mL; and uniformly mixing 2 to 9999 parts of mother liquid, 1 to 10 parts of surface active agents and 0 to 9997 parts of distilled water to obtain the growth regulating agent. The growth regulating agent is uniformly sprayed on the jatropha whole strain until liquid drops drip down before or after 10 days of the occurrence of the blossom buds. After the growth regulating agent is sprayed on the jatropha plants for 1 to 3 times during each flowering period, the generated total flower number, the female flower proportion, the fruit number and the seed yield are respectively 3.6 times, 4.3 times, 4.5 times and 3.9 times of those of untreated jatropha. At the same time, the oil content of the seed is also improved from 31.67 percent to 34.76 percent, and is improved by about 9.8 percent. The invention has the advantages of simple and convenient operation, low cost and obvious economic benefit, and has good application prospects.

The present invention relates to biological diesel oil and method for preparing same. The technical program is the biological diesel oil contains Jatropha crucas L. seed oil; in said Jatropha crucas L. seed oil, stearic acid content is not more than 0.4 (wt) percents. The method for preparing biological diesel oil comprises production art of the biological diesel oil and refining, improving art of the biological diesel oil; said refining, improving art comprises steps of: a, hydrating and degumming; b, alkali refining and acid removing; c, dehydrating; d intermittent deodorizing; e, emulsification and esterification. The invention has good effect of recucing stearic acid content in the Jatropha crucas L. seed oil, eliminating aromatic compound such as phthalic acid etc., improving burning ability of the biological diesel oil, reducing carbon accumulation in motor cylinder, improving power ability of motor, and discharging index. And the invention has simple producing process and low producing cost.

The present invention relates to a high-efficiency in vitro regeneration method of barbadosnut; the hypocotyls with the highest regeneration frequency are selected by comparing the regeneration frequencies of explants made of different parts of germfree seedlings of barbadosnut on the differential medium; and then the hypocotyls of the barbadosnut are taken as the explants to be separately inoculated into MS differential media of different incretion concentrations and the comparatively optimum inducement and regeneration medium prescription is that MS plus 1.0 mg / LBA plus 2.0 mg / LIAA; and among the radication results of barbodosnut tissue culture seedling induced on media of different concentrations, the radication result of the seedling induced on MS plus 1.0 mg / LIAA is the best. The establishment of the present invention of a high-efficiency in vitro regeneration method of barbadosnut can make the genetic transformation smoothly implemented and obtain a comparatively high transformation rate and effect; the method laid the foundation for barbadosnut breeding with the modern biotechnology means.

The present invention relates to a commercially viable process for in vitro mass culture of Jatropha curcas. The process for in vitro mass culture of Jatropha curcas is simple, faster, and suitable for production of disease-free root tubers of uniform quality and employs media with a reduced concentration of phytohormones.

The invention discloses a leprosy tree bacteria root nursery sock growing method, comprising the procedures of bacteria root agent preparation, seedling process, bacteria root agent inoculation, replanting and vaccination of seedling tubes. The invention is vaccinated with mycorrhizal fungi, which improves stress resistance and adaptability of leprosy trees. And the bacteriorhiza agent vaccination method has the advantages of little energy consumption, convenient employment, excellent production increase and extensive application scope, promotes the growing of leprosy trees and provides large numbers of high grade root bacteria seedlings for industrialized plantation of leprosy trees.

The invention discloses a high-viscosity fuel preheating system for a diesel engine, relating to the technical field of diesel engines of directly using high-viscosity fuel. The high-viscosity fuel preheating system comprises a heat exchanger (11), a temperature sensor (8), an exhaust control valve (12) and a controller (13), wherein an exhaust hole of an engine (1) is connected with an exhaust pipe (2) for exhausting waste gas, the exhaust pipe (2) is provided with a branch pipe and connected with the heat exchanger (11) through a heat exchanger air inlet pipe (4), and the exhaust control valve (12) is arranged on the heat exchanger air inlet pipe (4); a heat exchanger gas outlet pipe (5) is connected with a tail gas treatment device; and an oil tank (10) is connected with an oil inlet of the heat exchanger (11) through an oil pipe (6), and an oil outlet of the heat exchanger (11) is connected with an oil spraying pump (9) through the oil pipe (6) and introduced into the engine (1). In the invention, oils such as jatropha curcasl oil and the like are heated by using the heat exchanger with a heat energy exhausting function in a combustion chamber to ensure that viscosity and flowability as well as spraying condition of the oil spraying nozzle are improved.

The invention relates to a method for carrying out gene transformation on manioca by using Agrobacterium tumefaciens mediated transformation, which comprises the following steps: preparing a manioca explant and agrobacterium liquid, and coincubating the mainoca explant after being soaked by the agrobacterium; inserting the coincubated explant into a screening culture medium C13PC for culture to obtain a resistance callus, wherein the screening culture medium C13PC contains 1-3mg / L of Glufosinate ammonium or 1-10mg / L of hygromycin; transferring the obtained resistance callus into a differential medium, and differentiating adventitious buds; and obtaining a transgenosis plant through rooting culture. The method can successively lead in functional genes to carry out genetic improvement on a wild manioca. The conversion efficiency can be about 10% or even more than 10%; the transgenosis inheritance is stable, and the cost is low; a manioca in vitro has good regenerative system stability; and the method has simple operation and high regeneration efficiency, and the time from the induction of the callus to the obtain of the regeneration plant is only 80-90 days.

The jatropha yield increasing and stabilizing cultivation process includes the following steps: growing seedling; transplanting; pruning and shaping through topping-off and shaping to form turned umbrella tree after the jatropha tree grows to 1-1.5 m height and maintaining the tree height during blossom of 2-2.5 m and fruit bearing all the year round; and applying balancing nitrogen fertilizer, phosphate fertilizer and potash fertilizer. The cultivation process has fast growth, increased and stabilized yield, low consumption and easy picking of jatropha fruit.

The invention discloses a bio-organic fertilizer with shrimp shell powder as a main material. The bio-organic fertilizer is composed of, by weight, 57-60 parts of the shrimp shell powder, 10-12 parts of urea, 8-10 parts of jatropha leaf powder, 1-2 parts of distillers' grains, 20-23 parts of slaughter scraps, 6-8 parts of rape seed cakes, 2-3 parts of esliptae herba, 2-3 parts of sesbania, 4-5 parts of stevia rebaudiana, 1-2 parts of corundum powder, 3-4 parts of ditch mud, 6-7 parts of pepper stalk powder, 55-60 parts of swill, 9-13 parts of brown sugar and 72-80 parts of faeces of human and animals. The bio-organic fertilizer produced by the method has high organic matter content, and can improve soil quality and soil structure, thereby keeping ecological balance. Besides, the bio-organic fertilizer is comprehensive in nutrition and contains macroelements and microelements required by crops and a large amount of active substances produced during a microbial fermentation process.

The invention relates to a grafting method of soft hollow stalk plants. The scion and the rootstock in which the size and maturity are basically the same are selected. The grafting ends of the scion and rootstock are respectively cut into the slopes with the same gradient. One head of the sticker is used to insert into the scion hollow pith part, and the other head is inserted into the rootstock hollow pith part. After the slopes of the scion and rootstock are in tight anastomosis, the grafting opening is closely packed and fastened with bandages and the fracture surface of the scion upper end is well sealed. The survival rate of the grating method of the invention is more than 90%. The method of the invention can be promoted and applied in soft hollow stalk plants such as cassava, castor, jatropha, papaya to meet the goal of the improvement of product yield and product quality.

The invention discloses a jatropha curcas dedicated composite fertilizer, a preparation method and applications thereof. The dedicated composite fertilizer is prepared from the following components in parts by weight: 30 to 35 parts of banana stalk, 35 to 40 parts of manure of livestock and poultry, 5 to 12 parts of liquid monosodium glutamate dreg, 10 to 13 parts of peanut bran powder, 1 to 3 parts of jatropha bran powder, 0.02 to 2 parts of plant insecticide, 1 to 2 parts of water-retaining agent, 0.002 to 0.010 part of microbial inoculant, 1 to 2 parts of urea with a nitrogen content not less than 46.4%, 3 to 4 parts of calcium superphosphate with a P2O5 content not less than 12%, and 2 to 3 parts of potassium chloride with a K2O content not less than 60%. The provided fertilizer is suitable for being used in the seedling period and topdressing period and can be used as a base fertilizer. The jatropha curcas dedicated composite fertilizer has comprehensive and abundant nutrients, can provide enough nutrients for jatropha curcas, and is capable of preventing diseases and insects. Jatropha curcas is usually planted in water-deficient areas such as hillside, high mountains, and the like, and the provided fertilizer can relieve water deficiency. Moreover, the raw material source is wide, the cost is low, and the preparation technology is simple. So the fertilizer can promote the sustainable development of rock desertification region and generates good economic benefit, social benefit, and ecological benefit.

The invention relates to high-performance cyhalothrin pesticide aqueous emulsion and a preparation method thereof. The aqueous emulsion is prepared by mixing the following raw materials by weight percent: 2.5 to 10 percent of high-performance cyhalothrin, 10 to 70 percent of Jatropha seeds biodiesel, 3 to 10 percent of emulphor, 0.5 to 4 percent of high-performance cyhalothrin solvent and the balance of water. The pesticide aqueous emulsion has the advantages that the environment pollution is small, the characteristic is stable, the pesticide aqueous emulsion can be diluted by 2000 to 2500 times of water, can tolerate the temperature of 0 to 65 DEG C, the aqueous emulsion is not layered, the combination of the high-performance cyhalothrin and the Jatropha seeds biodiesel has synergism to the high-performance cyhalothrin, and the aqueous emulsion is suitable for large-scale generalization and application.

The present invention provides a delayed coking process comprising a step of subjecting a mixed feed comprises residual heavy hydrocarbon feedstock and bio oil obtained from fast pyrolysis of lignocellulosic biomass of one or more of Jatropha, Cashew nut, Karanjia and Neem to a delayed coking process and a system for the delayed coking process.

The present invention provides a novel glycerol-based heterogeneous solid acid catalyst by simultaneous partial carbonization and sulfonation of crude glycerol obtained as a by-product during the biodiesel process. Solid acid catalyst with similar activity is also prepared from glycerol pitch (by-product of fat splitting) and technical grade glycerol. These glycerol-based solid acid catalysts are employed for esterification of fatty acids and fatty acid present in the high and low free fatty acid (FFA) containing vegetable oils like rice bran, karanja and jatropha; fatty acid distillate; deodorizer distillate and acid oil which are being used as raw materials for the preparation of biodiesel. These catalysts are highly active, reusable and simplify the biodiesel process particularly for fatty acids or high FFA containing vegetable oils by replacing the traditional homogeneous mineral acid catalysts.

The invention relates to an Aspergillus versicolor for fermenting a Jatropha cake to manufacture biological bacterial manure, belonging to the field of manure. The Aspergillus versicolor provided by the invention is named as Aspergillus versicolor A003. The stain is preserved in a preservation unit designated by State Intellectual Property Office, the preservation date is 18th, July 2011, the name of the preservation unit is China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2011240. When the Aspergillus versicolor is inoculated on a culture medium mainly prepared from the Jatropha cake, the Jatropha cake can be quickly fermented, toxic components in the Jatropha cake can be eliminated, the detoxified Jatropha cake and live thalli growing on the Jatropha cake can be used as biological bacterial manure and can promote the growth of various crops.

The invention discloses Jatropha bio-aviation fuel refined oil, which is characterized in that in the refined oil, the unsaponifiable matter content is less than or equal to 0.2 percent, the metal ion content is less than or equal to 10 ppm, the phosphorus content is less than or equal to 3 ppm, the acid value is less than 5 ImgKOH / g, the moisture content is less than or equal to 1000 ppm, and the appearance is clear and transparent. The invention further discloses a preparation method of the Jatropha bio-aviation fuel refined oil. Due to the situations that on one hand, the method adopts an acidic modifying agent to convert nonhydratable phospholipid into hydratable phospholipid, and on the other hand, the method selects activated carbon with the aperture matched with the size of phospholipid and larger specific surface area and magnesium silicate, and additionally adopts succimer MEA, the nonhydratable phospholipid and metal ions and the like in Jatropha crude oil or in conventional Jatropha refined oil processed by the prior art can be effectively removed, and various performance indexes of the obtained refined oil achieve the standard for the production of the bio-aviation fuel refined oil, so that the possibility of large-scale production of Jatropha bio-aviation fuel is realized; and the method is simple and mature, has low investment cost, and is particularly suitable for industrial application.

The method of the present invention comprises: Obtaining an explant from the seeds of Jatropha curcas; Putting the explant derived from the seed of Jatropha curcas in a culture medium; Breaking the intercellular unions of the explants tissue, which generates individuals cells; Incubating for a determined time the culture medium with the generated individual cells, that were multiplied; and, Extracting oil from the cells that multiplied from the individual cells generated from the explants derived from the Jatropha curcas seed.

The present invention provides an improved process for the preparation of biodiesel from crude vegetable oils especially Jatropha (Jatropha curcas) and Karanja (Pongamia pinnata). In the preset invention the bio-active constituents, fatty acids and polar materials are selectively removed from the crude oils by liquid-liquid extraction with a polar solvent into the solvent phase in a non-destructive manner in specially designed equipment either in a batch mode or continuous mode and the oil left as residue is amenable for direct transesterification. After transesterification, the glycerol phase is separated and the crude biodiesel is neutralized, purified by adsorption of undesirable constituents like the residual glycerol, gums, soap, salts etc over a bed of adsorbent like Celite thereby circumventing the conventional water washing step. An alternative to the adsorbent method, the crude ester obtained after transesterification is purified using partition with aqueous solvent to biodiesel which meets the International specifications.

The invention relates to a mesotrione oil suspension agent composition. The composition is characterized in that, the composition comprises, by mass: 8-15% of mesotrione, 10-20% of an emulsifying dispersant, 1-3% of a suspension stabilizer, 1-5% of water, 0.1-0.2% of an organosilicon defoaming agent, and balance of a green and environment-friendly solvent. The green and environment-friendly solvent mainly comprises a mixture of dimethyl carbonate and at least one of jatropha pine nut oil and castor oil. According to the mesotrione oil suspension agent provided by the invention, non-edible plant oil with wide source and the non-toxic green solvent are adopted as suspension carriers, such that the composition is green and environment-friendly, and has a characteristic of high safety to plants. Also, a crystal transition inhibiting agent is added, such that mesotrione original drug crystal transition and decomposition can be effectively inhibited.

The invention discloses a shaping and pruning method of jatropha curcas, which comprises two steps of sapling pruning and cultivation pruning, wherein the saplings before planting are removed from the top and pruned when taking the seedlings, and during the 2-4 years of management and maintenance after cultivation Shaping and pruning should be carried out step by step. The method of the invention can make the main pole of Jatropha curcas single, dwarf the tree body to 2.5-3 meters, increase the number of branches to 5-6 levels, and form an "umbrella" structure, which is beneficial to sufficient photosynthesis, increases flowering and fruiting branches, Increase the amount of results by about 25-30%. Therefore, the present invention can provide technical guidance for tree body dwarfing, reasonable structure tree shape, and improvement of jatropha seed yield, and can generate huge ecological and economic benefits.

The invention relates to the technical field of plant clonal propagation, in particular to a method for raising seedlings by cutting of Jatropha curcas Linn, which comprises the steps of strips picking, seedbed preparation, lopping, cuttage, management after cuttage and management before afforestation, and is characterized in that the upper layer of the seedbed is a coarse river sand layer, and the lower layer thereof is a soil layer. When in cuttage, the part of the branch buried in the ground is cut into the river sand matrix of the upper layer, but is not suitable for rising over the river sand to penetrate into the soil layer. As the entire strip is in the coarse river sand, the good permeability of the strip is guaranteed, simultaneously the strip rotting probability caused by waterlogging is also greatly reduced, after taking root, the strip penetrates into the soil so as to absorb the soil nutrient well, thus ensuring the quality of nursery stock root system; simultaneously the rootage powder processing is not needed in strip cutting, and only the immersion and disinfection by using carbendazim is needed, thus reducing the cost and being a set of method which has simple operation and comparatively extensive management.

The invention discloses a preparation method of a compost for ganoderma lucidum cultivation. The preparation method comprises the steps of 1, smashing one or a combination of branches, leaves and shells of jatropha, and then performing biological detoxification, or performing biological detoxification on one of a combination of branches, leaves and shells of jatropha, and then smashing the one or the combination of the branches, the leaves and the shells of jatropha; 2, uniformly mixing and stirring 25-65 percent of the one or the combination of the branches, the leaves and the shells of jatropha subjected to biological detoxification, 20-50 percent of miscellaneous wood chips, cottonseed hulls or xylitol residues, 8-25 percent of wheat bran or rice bran, 0.05-1 percent of monopotassium phosphate and 0.2-3 percent of gypsum powder in water, and controlling the pH value to be in a range of 5.5-7.0 and the water content to be in a range of 65-75 percent, thus obtaining the compost. The compost prepared by the method disclosed by the invention utilizes resources fully, the growth of infectious microbes can be effectively controlled, and insect pests can be controlled; the internal quality of ganoderma lucidum cultured by the compost is higher than that of a product cultured in a field through other composts.

The invention discloses a bacteriostat from Jatropha curca, which contains Jatropha curcas extractive, wherein the Jatropha curcas extractive is prepared by using the method comprising the following steps: collecting syrup by choosing and cutting a strong luxuriant Jatropha curcas branch; preserving the syrup for 6 hours at 3-7 DEG C, and clarifying the syrup for 12-24 hours; taking the emulsion at lower layer, thereby acquiring original pulp of a Jatropha curcas tree; or washing and disinfecting fresh leaves of the Jatropha curcas tree; squeezing or smashing the leaves, adding water and soaking the leaves for 12-72 hours; and then filtering and concentrating the filtrate, thereby acquiring a water extract of Jatropha curcas leaves. The invention also discloses a bacteriostasis application of the bacteriostat in planting edible mushrooms. The bacteriostat from Jatropha curcas provided by the invention can be used for restraining fungus which is fostered by bacteria, has ultra-strong sterilization feature, and is characterized by full nutriments. The bacteriostat powder agent from Jatropha curcas which is scientifically prepared has excellent effect of restraining mildew and bacteria in planting edible mushrooms, is free from undesirable impact on the growth of fungus mycelium, and has excellent selective bacteriostasis.

The invention relates to a jatropha polyploidy induction method in the condition of isolated culture and is divided into such steps as jatropha aseptic seedling cultivation, polyploidy induction, polyploidy regenerated bud cultivation, selecting stable variants and rooting induction of variants in rooting cultivation medium containing active carbon, seedling adaptation in matrix and transport of seedling to land after survival. By adopting colchicines to induce the polyploidy of chromosome under isolated condition, the invention can easily control test conditions and repeat test result, thereby improving working efficiency, obtaining a plurality of plants with polyploidy chromosomes and reducing or avoiding heteroploidy cell chimerism. The invention also has actual significance in improving stress resistance of jatropha and can promote the development of renewable resources of jatropha.

The invention relates to a method for efficiently producing jatropha M_2 seeds processed by EMS (methanesulfonic acid ethyl ester), which comprises the following steps: a preprocessing step: collecting jatropha seeds, and preprocessing the jatropha seeds; a mutagenizing step: mutagenizing the jatropha seeds for an appropriate time by using an EMS solution (with appropriate concentration), and in the process of chemical mutagenizing, simultaneously mixing the seeds with the solution so as to ensure that the EMS solution is uniform in concentration and the jatropha seeds can be fully contacted with the EMS solution; a seedling raising and transplanting step: carrying out seedling raising and timely transplanting on the jatropha M_1 seeds subjected to mutagenizing so as to cultivate jatropha M_1 plants; and a self-pollination step: after the cultivated jatropha M_1 plants reach the flowering stage, carrying out self-pollination on the jatropha M_1 plants, after the fruits of the jatropha M_1 plants get ripe, obtaining the jatropha M_2 seeds. The method in the invention has the advantages that an ethylmethane sulfonate is used as a mutagen for mutagenizing the jatropha seeds, the preprocessing step and the mixing and self-pollination are adopted in the process of mutagenizing, the jatropha M_2 seeds processed by EMS can rapidly be obtained.