High-efficiency isolated regeneration method of physic nut

Abstract

The present invention relates to a high-efficiency in vitro regeneration method of barbadosnut; the hypocotyls with the highest regeneration frequency are selected by comparing the regeneration frequencies of explants made of different parts of germfree seedlings of barbadosnut on the differential medium; and then the hypocotyls of the barbadosnut are taken as the explants to be separately inoculated into MS differential media of different incretion concentrations and the comparatively optimum inducement and regeneration medium prescription is that MS plus 1.0 mg / LBA plus 2.0 mg / LIAA; and among the radication results of barbodosnut tissue culture seedling induced on media of different concentrations, the radication result of the seedling induced on MS plus 1.0 mg / LIAA is the best. The establishment of the present invention of a high-efficiency in vitro regeneration method of barbadosnut can make the genetic transformation smoothly implemented and obtain a comparatively high transformation rate and effect; the method laid the foundation for barbadosnut breeding with the modern biotechnology means.

Description

Technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for efficient in vitro regeneration of Jatropha curcas. Background technique [0002] Jatropha, also known as fake peanut, belongs to the semi-succulent small tree or large shrub of Euphorbiaceae. The whole plant is poisonous. The stems, leaves, and bark are all rich in white milk, which contains a lot of toxic protein. The concentration of seed toxic protein is the highest, and its toxicity is similar to that of ricin. Jatropha seeds can be extracted with clear golden liquid fuel oil after pressing and lipidation treatment. The oil content is about 60%, which exceeds that of soybeans, rapeseed and other oil crops. This new type of fuel is suitable for all kinds of diesel engines, and is superior to domestic zero diesel in terms of sulfur content, carbon monoxide and lead emissions and other technologies. It is a low-cost and highly environmentally friendly fuel. Mixin...

AI Technical Summary

Problems solved by technology

[0005] Because Jatropha has only been paid attention to by people in recent years, the breeding of Jatropha has just started in my country. Since Jatropha is mostly in the wild state, the genetic resources are simple, and because it is a woody plant, it is quite difficult to change its genetic traits by conventional breeding.