The invention discloses a method for preparing biological diesel by converting 
biology to 
grease in a non-
solvent system, belonging to the biological diesel composition technical field. In the non-
solvent system, the invention converts all 
grease to produce the biological diesel by using 
Rhizopus chinensis CCTCC M201021 full 
cell lipase; all 
grease can be converted by batching fed-batch short chain 
alcohol, which prepares the biological diesel and the 
by product-glycerin, the yield of the biological diesel is 95 percent. The invention uses low cost grease which are mainly waste resources such as the unedible 
Jatropha curcas seed oil, the waster oil in the restaurant industry and the 
acid value oil, etc. as raw materials, thereby enlarging the material sources; because the 
Rhizopus chinensis CCTCC M201021 full 
cell lipase which is extremely low in cost is used as the catalyst, the problem of high price catalyst is effectively resolved; the toxic action of the 
alcohol on the biological catalyst is resolved by the batching fed-batch the short chain 
alcohol, thereby improving the conversion rate of the short chain alcohol. On the basis of the product quality reaching the 
international standard of the biological diesel, the cost of producing the biological diesel by the biological 
enzyme method is greatly lowered.