57 results about "Vernicia fordii" patented technology

The invention discloses a method for regeneration plant of tung oil tree leaf, and belongs to the technology field of rapid propagation of tung oil tree. The method comprises the steps of adventitious bud induction directly by tung oil tree leaf, subculture, multiplication culture, strong seedlings culture, rooting culture, acclimatization and transplantation, which not only provides a path for rapid breeding of excellent tung oil tree plants, and lays a solid foundation for improving resistance of the tung oil tree, tung oil tree output and properties of oil quality later on, and for building a tung oil tree genetic system.

The invention discloses a plant source pesticide for efficiently preventing and controlling main golden autumn pear diseases and insect pests (psylla chinensis, pear yellow meal aphid, schizaphis piricola, fruit piercing moth and pear black spot). The plant source pesticide is mainly prepared from ginkgo leaf, stem and leaf of tung tree, oleander leaf, arborvitae leaf, Chinese red pine leaf, litsea cubeba fruit, tea-oil tree residue cake, marigold root and sabina vulgaris leaf by extracting and processing. Meanwhile, the invention discloses a preparation method and application of the plant source pesticide, and belongs to the technical field of plant protection. The plant source pesticide can efficiently prevent and control the main golden autumn pear diseases and insect pests such as psylla chinensis, pear yellow meal aphid, schizaphis piricola, fruit piercing moth and the like, can effectively inhibit the growth of pathogenic bacteria of the pear black spot of the golden autumn pears at the same time, and has good environmental safety and good development and application prospects.

The invention discloses an agrobacterium-mediated jatropha curcas gene transformation method. A material after 1/3 to 1/4 of base is cut from the cotyledon of a mature seed of jatropha curcas is used as an agrobacterium infection receptor. The method comprises the following steps of: after co-culturing, inoculating the transformed receptor and the agrobacterium to a culture medium without a screening agent to perform callus induced culture for 4 weeks; and succeeding the receptor and the agrobacterium to a selective culture medium and screening resistant buds by using kanamycin sulfate as a screening agent, sub-culturing the resistant buds obtained by screening to obtain resistant plants, and obtaining transgenic jatropha curcas plants by molecular detection. Compared with the conventional jatropha curcas transformation method, the method can obtain high regenerative bud induction rate, simplifies the transformation program, shortens the transformation period, greatly improves the transformation efficiency and has good application prospect.

The invention discloses a method for carrying out asexual cuttage propagation on vernicia fordii, belonging to the technical field of asexual propagation of plants. The method comprises the following steps of: (1) branch collecting time; (2) seedbed preparation and cuttage environment; (3) selection of cuttings and treatment of cutting branches; (4) pretreatment of a growth regulator; (5) cuttage; and (6) management after cuttage. The method provided by the invention is simple and feasible, has the advantages of low cost and short cycle and can conduce to realization of effective and rapid asexual propagation of the vernicia fordii. The method has the beneficial effect of solving the problem that the vernicia fordii can not root during cuttage, and has very important effects on and significance for accelerating popularization of excellent clone of the vernicia fordii and promoting construction of biodiesel raw material forests.

The invention discloses a Vernicia fordii planting device and relates to the technical field of crop planting. The Vernicia fordii planting device comprises a base and is characterized in that a screwis rotatably connected to the top of the base, a first bevel gear is fixedly mounted on the surface of the screw, a first motor located on the left side of the screw is fixedly mounted at the top ofthe base, a second bevel gear is fixedly mounted at the right end of the output shaft of the first motor, one side of the second bevel gear meshes with the first bevel gear, a guide column located onthe right side of the screw is fixedly mounted at the top of the base, a motor box is slidably connected to the surface of the guide column through a slide sleeve. The Vernicia fordii planting devicehas the advantages that the first motor drives the second bevel gear to rotate, the screw is driven to rotate by the first bevel gear meshing with the second bevel gear to allow a threaded sleeve to drive the motor box to move vertically, a second motor drives a drill bit to rotate at the same time to drill a pit, which is used for planting a Vernicia fordii seedling later, in the ground; the Vernicia fordii planting device is simple and convenient to operate and is unaffected by soil hardness, the drilled pits are consistent in size, and planting is facilitated.

The invention discloses a method for extracting genomic DNA from mature and old tung oil tree leaves, relating to the separation and extraction technology of genomic DNA from plants in the molecular biology experiments. The method mainly comprises the steps of leaf tissue sample pretreatment, cell disruption, non-DNA component extraction, DNA precipitation, ion removal and tissue sample cyclic extraction. The method has the following advantages and positive effects: polyphenols, polysaccharides and other secondary metabolites in which the leaves are rich can be effectively removed by using washing liquid to pretreat the tissue samples of the mature and old tung oil tree leaves once; the step of tissue sample cyclic extraction is absent in the traditional methods for extracting genomic DNA; one tissue sample can undergo genomic DNA extraction four times, thus obviously increasing the yield of the genomic DNA; and the genomic DNA with high quality and high yield can be extracted from the mature and old leaves stored at low temperature for a long time by the method. The method is suitable for extraction of genomic DNA from mature and old tung oil tree leaves.

The invention discloses a bacillus pumilus strain. The bacillus pumilus strain is preserved in the Guangdong culture collection center on June 5, 2018, is named as bacillus pumilus CFC-5, and has a preservation number of GDMCC NO:60397. The bacillus pumilus CFC-5 strain is able to degrade protein in tung cake, has a broad spectrum inhibition effect for soil-borne diseases of crops, has a growth promoting effect and provides a strain source to tung cake degrading. The invention further discloses application of the strain in degrading protein in tung cake and inhibiting crop diseases.

The invention discloses an olive xenoplastic grafting method comprising the following steps of (1) preparing tung tree stock, (2) selecting scions,(3) grafting and (4) managing. After grafting, soil has to be maintained moisty and moisture is prevented from invading a joint; when the olive sprouts on the stock under a grafting joint, the sprouts have to be immediately eliminated; terminal buds on main branches and side branches on the stock over the grafting joint have to be eliminated; and the stock has to be cut immediately when scions sprouts. The olive xenoplastic grafting method further comprises step (5) in which, when grafting seedlings grow to one meter, the top is broken; and two to three pairs of buds are remained for continuously growing into main branches. In the method, tung trees are employed for stock grafting olive; the tung trees has fast growth speed, can prevent diseases and pests and resist drought and waterlogging; high grafting rate can be achieved with more than 88%; a new method is provided for olive grafting; and the olive xenoplastic grafting method is simple, easy to operate and suitable for large-scale promotion and use.

The invention discloses a method for directly inducing plant regeneration by using Vernicia fordii hypocotyl as an explant, comprising the following steps: using hypocotyl of a Vernicia fordii seed aseptic seedling as an explant, directly inducing an adventitious bud, proliferating and elongation culturing the adventitious bud, and performing rooting culturing, domestication and transplanting to obtain a robust regenerated plant of Vernicia fordii. Compared with the prior art, the method has the advantages such as regeneration process simplicity, hormone addition singleness, operation convenience and relatively short regeneration cycle. A new technical means to rapidly propagate Vernicia fordii is provided, and also certain technical support is provided for genetically improving Vernicia fordii by genetic engineering means in future.

The present invention discloses a silkworm excrement cultivation media and a preparation method thereof, and belongs to the technical field of agriculture, wherein silkworm excrement, plant ash, vernicia fordii bran and sand-soil are uniformly mixed according to a volume ratio of 50:15:15:20, stacking retting fermentation is performed for usually 30-40 days, screening is performed after fermentation composting, and then uniform mixing is performed to obtain the silkworm excrement cultivation media. According to the present invention, the main raw material is the silkworm excrement and is rich in the mulberry planting and sericulture area, the silkworm excrement cultivation media can be widely used for planting of vegetables, flowers and other crops, can provide a rich nutrition for crops, and has effects of soil-borne disease reducing, quality and yield improving, solving of the environmental problems of agricultural waste, resource recycling achieving, a certain economic benefit, and a certain ecological benefit.

The invention discloses a method for extracting genomic DNA from young tung oil tree leaves, relating to the separation and extraction technology of genomic DNA from plants in the molecular biology experiments. The method mainly comprises the steps of leaf tissue sample pretreatment, cell disruption, non-DNA component extraction, DNA precipitation, ion removal and tissue sample cyclic extraction.The method has the following advantages and positive effects: proteins, polyphenols, polysaccharides and other secondary metabolites in which the leaves are rich can be effectively removed by using washing liquid to pretreat the tissue samples of the young tung oil tree leaves three times; the step of tissue sample cyclic extraction is absent in the traditional methods for extracting genomic DNA;one tissue sample can undergo genomic DNA extraction four times, thus obviously increasing the yield of the genomic DNA; and the genomic DNA with high yield and high quality can be extracted from theyoung leaves stored at low temperature for a long time and from fresh young leaves acquired at different growth temperatures by the method. The method is suitable for extraction of genomic DNA from young tung oil tree leaves.

The invention discloses a method for efficiently and directly reproducing plants by virtue of vernicia fordii petioles, and belongs to the field of plant cell engineering. The method mainly comprises the processes of direct induction of adventitious buds by virtue of the vernicia fordii petioles, secondary reproduction culturing, strong seedling culturing, rooting culturing, acclimatization and transplantation and the like. According to the method, means is provided for the rapid reproduction of high-quality vernicia fordii plants, a solid foundation is also laid for improving the resistance, the yield of vernicia fordii and characters such as oil quality and the like by virtue of a genetic engineering method and establishing a vernicia fordii genetic system, and many means and technical support are provided for the culturing of transgenic vernicia fordii.

The invention discloses a tissue culture and rapid propagation method suitable for tung trees. The method comprises the following processes: selecting explants, treating the explants, carrying out primary bud induction culture, carrying out propagation culture, culturing strong buds and carrying out rooting culture; the method comprises the concrete steps of collecting semi-lignified tung tree twigs, which grow robustly in the very year, as the explants, carrying out pruning and sterilizing treatment on the explants, and then inoculating an initial bud induction medium with the explants so as to obtain initial buds; inoculating a propagation medium with the initial buds, and carrying out the propagation culture for 3-4 cycles to form multiple shoots; inoculating a strong bud culture medium with the multiple shoots for culturing; finally, inserting a single bud with the height of more than or equal to 2cm into a rooting medium for inducing a root. After the tissue culture and rapid propagation method is used, the seedling raising period of the tung trees is shortened, and seedling raising materials are saved; the method solves the problems that the traditional seedling raising method needs more seedling raising materials, is long in cycle, and the like, greatly lowers the production cost, increases the production efficiency, and has better economic benefit, social benefit and ecological benefit.

The invention discloses a soil modifying fertilizer comprising the following components, by weight, 30-50 parts of biogas residue, 10-20 parts of soybean dregs, 5-10 parts of bone meal, 5-10 parts of a residue from oil extraction by Vernicia fordii, 10-15 parts of stable manure, 1-2 parts of a bio-inoculant, 7-12 parts of brewing residue of cassava and 1-5 parts of plant ash. The invention also provides a preparation method of the soil modifying fertilizer. The soil modifying fertilizer, compared with the prior art, can effectively improve properties of soil and improve fertility of soil. The raw materials of the soil modifying fertilizer are low in cost and easy to obtain. The fertilizer is simple in process and is easy to popularize.

The invention discloses a method for improving disease resistance, lodging resistance and yield of vernicia fordii. According to the method, vernicia fordii is grafted by adopting side grafting, split grafting with top bud and other methods, so that variety improvement of vernicia fordii is quickly realized, and high quality, high yield, disease resistance, lodging resistance and yield increase of vernicia fordii can be realized. By adopting the method, the improved variety has excellent resistance to fusarium oxysporum schl, root rot, giant mealy bug and other diseases; the life of grafted vernicia fordii pure plantation is over 30 years, which is improved by 5-6 times that of conventional pure plantation; the lodging resistance of vernicia fordii is increased by over 30 percent in comparison with that of conventional varieties; and the yield is increased by about 10 percent. The method is applicable to large-scale field cultivation, and construction of about 10,000mu demonstration base is completed by adopting the method.

The invention discloses a pruning and planting method of Chinese prickly ash. The steps are as follows: 1) soil preparation; 2) seedbed selection; 3) seedling cultivation; 4) pruning management: (1) pruning and fixed poles in the first year; (2) second year Pruning and cultivating secondary main branches, pinching and cultivating fruiting branches; (3) pruning and cultivating the extended branches and side branches of the main branches in the second year, cultivating fruiting branches and fruiting groups; After the pepper insecticide composed of tung tree juice water and rice washing water is proportioned, it is blended with clean water at a ratio of 1:100, and sprayed once a day with a sprayer at the place where the pests and diseases occur on a cloudy day, and sprayed continuously for 2 days. God; the invention is beneficial to the firm skeleton of the peppercorns, robust branches, strong photosynthesis, good ventilation, spraying peppercorn pesticides, reducing pests and diseases, increasing the yield of peppercorns, and increasing the yield by more than 30%.

The invention discloses a tung tree cultivation method. The method comprises the steps that 1, seedling raising is conducted; 2, when planting is conducted in spring, gentle slopes which are loose in soil, exposed to sun, good in drainage and high in content of organic matter are selected, and the height above the sea level is controlled to range from 300 m to 900 m; 3, soil preparation and planting are conducted; 4, when planting is conducted, lifting is conducted gently after oil covering is conducted twice, covering is conducted till soil exceeds the portion 2-3 cm away from a mud door heart, and watering is conducted in time; 5, crops such as soybeans and peanuts are planted between the lines of planted tung trees; 6, when the height of oil tree seedlings reaches one meter, apical dominance is removed, first level branching is promoted at about one meter, the centremost branch is selected as a trunk of second level branches, when the height reaches 60 cm to 80 cm, second-level branching is promoted, the centremost branch is reserved to serve as a third level branch, when third level branching is completed, topping is conducted on all the branches, and the branches are controlled to longitudinally grow; 7, according to growth vigor and land masses of different plants, before flowering, a nitrogenous fertilizer and a potash fertilizer are mainly applied, and an appropriate amount of micro-element fertilizers are matched.

The invention relates to a preparation method for a biomass charcoal anti-schistosoma cercaria slow-release pharmaceutical preparation. The preparation method comprises the following procedures: selecting the biomass of duckweed, poplar, vernicia fordii, bamboo powder and styphnolobium japonicum as carbonization materials; carrying out carbonization at 350-1000 DEG C to obtain appropriate biomass charcoal materials of which the specific surface area is 5-500 m<2>/g and the pore diameter is 0.5-10 nm; conducting functional group, surface appearance and specific surface area representation on the biomass charcoal materials; carrying out synthesis to obtain an anti-schistosoma cercaria pharmaceutical polyether benzamide; selecting the carbonized materials of bamboo powder, poplar and vernicia fordii as slow-release carriers of the anti-schistosoma cercaria pharmaceutical polyether benzamide, and measuring the release kinetic curve of polyether benzamide with the ultraviolet absorption spectrum; carrying out cercaria killing experiments. The preparation method has the advantages that the carbonized materials of bamboo powder, poplar and vernicia fordii are selected as the slow-release carriers of the anti-schistosoma cercaria pharmaceutical, so that the release speed of the pharmaceutical polyether benzamide can be slowed down, the slow-release period lasts for 4 days or longer, and the cercaria killing effect reaches 95% or above.

The invention discloses a trenching device for planting tung oil trees. The trenching device for planting the tung oil trees comprises a bottom plate, wherein a motor box is fixedly connected at the top of the bottom plate, a first motor is fixedly connected at the bottom of the inner wall of the motor box through a support plate, the outer surface of an output shaft of the first motor is sleevedwith a first pulley, a fixed block is fixedly connected at the bottom of the bottom plate, a rotating shaft is rotationally connected on one side of the fixed block through a bearing, the outer surface of the rotating shaft is sleeved with a second pulley, the outer surface of the second pulley is in transmission connection with that of the first pulley through a belt, one end of the rotating shaft penetrates through the fixed block and extends to one side of the fixed block, and the outer surface, extending to one side of the fixed block, of the rotating shaft is sleeved with a rotating gear.The invention relates to the technical field of planting equipment. The trenching device for planting the tung oil trees, disclosed by the invention, does not need manual trenching, improves the planting efficiency of the tung oil trees, reduces the labor force and labor time, and has very good practicability.

The invention relates to a complex insecticide of a tung tree leaf extractive and Metarhizium anisopliae and a preparation method thereof. The complex insecticide is prepared by blending the following raw materials by mass percent: 25%-65% of tung tree leaf extractive, 8%-32% of Metarhizium anisopliae conidial powder, 8%-20% of filling, 8%-20% of surfactant, and 4%-20% of insecticide adjuvant. The invention also comprises the preparation method of the complex insecticide. The complex insecticide provided by the invention has the advantages of high activity, stable effect, small influence on environment, rapidness of insecticidal efficacy and convenience in use, and can not only overcome the defect of poor fast-acting property of microbial pesticides, but also improve the insecticidal efficacy of a botanical insecticide. Experiments prove that after the complex insecticide diluted in 1000 times of water is sprayed, the average insecticidal rate can be over 84% after 72 hours.

The invention discloses a high-yield vernicia fordii seedling cultivation method. The cultivation method comprises the following steps of storage: selecting seed tree seeds with high oil yield for storage; seed selection: selecting large and full seeds without mildew spots and wormholes; soaking: selecting a room, putting the seeds into warm water with the temperature of 30-40 DEG C, adding hymexazol powder into the warm water, diluting the hymexazol powder by 400-800 times, soaking for one day per day, replacing the water, putting rooting powder, soaking for one day per day again, diluting the rooting powder to one thousandth, humidifying the surfaces of seeds by a humidifier when the rooting powder is exposed at night, keeping the indoor temperature at 30-40 DEG C, and finally, selecting the seeds sinking to the water bottom; and sowing, wherein 1-2 immersed seeds are put into each bag of nutrient soil. According to the method, large and full seeds without mildew spots and wormholes are screened and soaked, so that the budding time difference of different seeds is shortened, management is convenient, the quality of vernicia fordii seedlings is effectively improved, the transplanting survival rate is high, the vernicia fordii seedlings grow fast, the oil yield is high, and the method is relatively economical.

The invention discloses vernicia fordii seed husking equipment, and belongs to the field of deep processing of vernicia fordii seeds. The equipment comprises a hull breaking box, a feeding pipe is fixedly connected in a straight hole of the hull breaking box, the hull breaking box is provided with a hull breaking structure below the feeding pipe, and the hull breaking structure comprises a drivingmotor, a belt, a driving belt pulley, a driven belt pulley, a hull breaking roller and a fixed roller. The output end of the driving motor is fixedly connected with the driving belt wheel, the driving belt wheel is movably connected with the belt, the belt is movably connected with the driven belt wheel, the left end and the right end of the hull breaking roller are fixedly connected with a connecting shaft, and the connecting shaft is fixedly connected with the driven belt wheel. The front end of the hull breaking roller is provided with the fixed roller used in cooperation with the hull breaking roller, the fixed roller is connected with a width adjusting structure, and an extrusion structure is arranged above the hull breaking roller. The equipment is suitable for hull breaking treatment of vernicia fordii of different sizes by adjusting the distance between the fixed roller and the hull breaking roller, and subsequent use is facilitated.