30 results about "Psylla" patented technology

The invention relates to the technical field of pest catching, and particularly discloses a phylloxera catching device for lycium barbarum trees, comprising: an annular frame, the annular frame comprises a semi-ring sleeve, the semi-ring sleeve is provided with a first mounting port, and the first mounting port forms a closed annular structure through a closing member mounted on the semi-ring sleeve; the insect catching cover comprises two semi-ring covers, the diameter of the semi-ring covers is larger than that of the annular frame, and the two semi-ring covers are detachably spliced to form an annular structure; the insect catching cover is arranged on the surface of the annular frame in a sleeving mode. Through the assembling mode of the annular frame and the insect catching cover relative to the Chinese wolfberry trees, the tree shapes of the existing planted Chinese wolfberry trees are effectively utilized, and the farmland installation space is saved. The wind direction is sensed through the wind direction sensing assembly, and the insect catching cover can rotate along with the wind direction in cooperation with the driving assembly, so that the second insect inlet hole in the insect catching cover is located on the leeward side all the time, and the phylloxera can be effectively caught in windy weather through the wind sheltering performance of the phylloxera.

The invention belongs to the technical field of genetic engineering, and particularly relates to application of diaphorina citri transferrin Tsf in prevention and control of citrus huanglongbing. The invention provides an application of diaphorina citri transferrin Tsf in prevention and control of citrus huanglongbing. A nucleotide sequence for coding a gene of the diaphorina citri transferrin Tsf is shown as SEQ ID NO.1. The invention further provides a preparation method of the diaphorina citri transferrin Tsf. According to the invention, the expression of transferrin Tsf in diaphorina citri can be interfered, so that the infection ability of Candidatus Liberobacter asiaticum can be reduced, the bacterium carrying amount can be reduced, a new strategy is provided for effective prevention and control of the Candidatus Liberobacter asiaticum, and a new prevention and control idea is provided for controlling propagation of bacterial pathogens by means of vector insects.

The invention provides a navel orange disease and insect pest comprehensive prevention and control method based on a full-net-covering facility and application thereof, and relates to the technical field of agricultural disease and insect pest prevention and control. Comprising the following steps that a net shed is built in a navel orange planting area in a full net covering mode, and a 30% spirodiclofen and SYP-9625 suspending agent is selected for preventing and treating citrus red mites. According to the method, the net shed of a full-net-covering facility is built for physically preventing and controlling the diaphorina citri, the diaphorina citri is completely prevented from entering the net, the transmission medium of the Candidatus Liberobacter asiaticum is cut off, the dangerous disease of the Candidatus Liberobacter asiaticum is stably and completely eradicated, and the harm of the Candidatus red mites in the net shed is low by controlling the Candidatus red mites through efficient chemical agents. Through the synergistic effect of the two prevention and control, diseases and insect pests seriously harmful to citrus can be effectively controlled, the comprehensive prevention and control effect is remarkably improved, the yield and quality of citrus fruits are improved, and remarkable economic, social and ecological benefits are achieved.

The application discloses a kind of citrus psylla intelligent detection method and device based on deep learning, method includes the following steps: S1, acquisition citrus psylla color image under natural orchard scene, pre-treatment is carried out to acquisition image, and citrus psylla dataset is made;S2, improvement YOLOv5-s network model, using citrus psylla dataset is iteratively trained to improved YOLOv5-s network model, and optimal model is obtained and used as psyllid detection model;S3, using 2 pairs of servo steering engine is formed rotatable holder and carries variable zoom camera and forms image collector;Psyllid detection model is deployed to embedded device, and using the equipment control image collector;S4, the image obtained by image collector is input into psyllid detection model and is identified, determines whether there is citrus psylla in the image photographed and its position.The method and device of the application can be deployed in natural orchard, and the citrus plants in a certain distance range are efficiently and intelligently monitored to determine whether there is citrus psylla.

The application discloses DcPDF The application discloses application of a gene and a dsRNA in preventing and treating citrus psylla DcPDF The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The application can significantly inhibit the development of the ovary of a female citrus psylla and significantly reduce the oviposition amount by feeding the citrus psylla with a dsRNA targeting a nucleotide sequence shown as SEQ ID NO. 13 to reduce the expression level of a neuropeptide pigment dispersing factor gene. The application provides a new efficient target for green prevention and treatment of citrus psylla based on RNA interference technology, and helps to realize pesticide reduction and efficiency increase, and delay development of pest resistance.

The application discloses a carvyl composition for preventing and treating psyllospora, and belongs to the technical field of disease and pest control. The composition comprises the following components in parts by weight: carvyl 1-10 parts, neem essential oil 1-20 parts, alkyl polyglycoside 1-15 parts, cationic surfactant 1-15 parts, and stabilizer 1-15 parts. The carvyl and the neem essential oil in the application are both natural insecticides, and as main insecticidal components, the two are combined together to have a synergistic effect on preventing and treating psyllospora, and achieve excellent insecticidal effect. The specific cationic surfactant and the non-ionic surfactant alkyl polyglycoside are added, and the two have a synergistic effect, are dissolved in water during use, can form a micellar state in water, wrap the carvyl and the neem essential oil therein, make the carvyl and the neem essential oil have a slow-release effect, can improve the killing effect of the overall composition on psyllospora adults, can also wrap and kill the eggs of the psyllospora, and prevent continuous outbreak of the psyllospora.

The invention discloses an application of a DcPDF gene and dsRNA thereof in prevention and treatment of diaphorina citri. By feeding diaphorina citri with dsRNA with a targeting nucleotide sequence as shown in SEQ ID NO.13, the expression level of the neuropeptide pigment dispersing factor gene DcPDF (the nucleotide sequence is as shown in SEQ ID NO.1) is reduced, development of ovary of female diaphorina citri can be remarkably inhibited, and the egg laying amount is remarkably reduced. The invention provides a new efficient target for green prevention and control of diaphorina citri based on an RNA interference technology, is beneficial to realizing pesticide reduction and synergism, and delays the development of pest resistance to drugs.

The invention discloses a RanGAP1 gene and an application of the RanGAP1 gene in regulating and controlling the reproduction of female diaphorina citri. The nucleotide sequence of the DcRanGAP1 gene is shown as SEQ ID NO.9. After female diaphorina citri subjected to eclosion for 8 days down-regulates expression of the DcRanGAP1 gene, the early stage of egg laying is remarkably prolonged, the egg laying period is remarkably shortened, the egg laying amount is remarkably reduced, development of an ovary is remarkably inhibited, and the titer of the Huanglongbing in the ovary is remarkably reduced, so that the purpose of preventing and treating the diaphorina citri is achieved, and the diaphorina citri can be used for preventing and treating the diaphorina citri. A new thought is provided for preventing and treating the Huanglongbing.

The utility model discloses a citrus psylla attractant slow -release device, solve the problem that present device cannot switch sealed and release state. The device includes bottle body, the cover plate of hinged in the bottle mouth and the connecting cylinder, and the connecting cylinder is sealed through the cooperation of first sealing protrusion and bottom first limit slot when being inserted into bottle body completely, and is matched with middle part second limit slot when being half inserted, and its aeration structure and volatile hole are aligned to release attractant. The bottle mouth is equipped with volatile groove, and the cover plate connecting seat and the bottle mouth interference fit enhance the sealing property. The device switches state through the insertion depth of connecting cylinder, and is sealed and prevents leakage when transporting, and is uniformly slow -released when using, avoids the defect that traditional empty bottle transportation and release are uncontrollable, realizes "transportation seal -use release " automatic switching, promotes the operation convenience, accurately control release rate, enhances the trapping effect, is applicable to citrus psylla control.

The invention relates to the technical field of agriculture, and particularly discloses a method for preventing citrus canker and phylloxera and application. According to the method, different prevention agents are sprayed in the spring shoot period, the flowering period, the late summer shoot period and the autumn shoot period of plants respectively, so that the purpose of efficiently preventing and treating citrus canker and phylloxera is achieved. By means of the method for preventing the citrus canker and the phylloxera, the citrus canker and the phylloxera can be effectively prevented, plants suffering from the citrus canker and the phylloxera can be treated, and the method is easy and convenient to operate and free of toxic and side effects. The invention effectively solves the problems that a single chemical agent is easy to generate drug resistance in the prior art, the research and development cost of a biological agent is high, and the prevention and treatment effects of the chemical agent and the biological agent are limited.

The application discloses DcFoxO A gene and application thereof in preventing and treating Toxoptera citricola. DcFoxO The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The application discloses a gene of Toxoptera citricola DcFoxO The gene is highly expressed in the midgut of Toxoptera citricola, and the gene is targeted by feeding dsRNA to Toxoptera citricola DcFoxO It is found that the oviposition period of a female Toxoptera citricola is shortened, the oviposition amount is reduced, the ovary development is abnormal, and the titre of Candidatus Liberibacter asiaticum is reduced, the prevention and treatment effect on Toxoptera citricola is remarkable, and the gene has a good application prospect.

The present application belongs to the technical field of fruit tree disease prevention and control, and particularly relates to a method for removing sporadic citrus Huanglongbing trees in citrus orchards. According to the epidemiological characteristics of citrus Huanglongbing, the control strategy of 'completely removing diseased plants, strictly preventing and controlling citrus psyllids, and comprehensively preventing and controlling' is implemented at sporadic disease points in the diffusion front area. The sporadic disease point orchards are promptly cleaned of diseased trees, and the diffusion front area is comprehensively prevented and controlled to prevent the disease from occurring again. In this way, disease information and risk warnings can be promptly released, the removal of diseased trees and the strict prevention and control of citrus psyllids can be achieved, the invasion and reproduction of exogenous psyllids can be hindered, the risk of healthy seedlings getting sick can be reduced, and the spread of citrus Huanglongbing can be blocked. The present application provides technical support for delaying the further spread of citrus Huanglongbing and saves human and material resources.

The invention relates to the technical field of pesticides, and discloses an insecticidal composition containing ethiprole and flonicamid, which is characterized in that the ethiprole and the flonicamid are embedded in biodegradable polylactic acid-glycolic acid copolymer microcapsules; the microcapsules are suspended and dispersed in an aqueous medium containing an acrylic resin film-forming agent, an ultraviolet light absorber and agriculturally acceptable auxiliary materials. Through multi-layer protection and controlled release, the pesticide effect lasting period is effectively prolonged to 15-20 days, the application frequency and the dosage are reduced, and long-acting, efficient and environment-friendly pest control is achieved. The composition disclosed by the invention has excellent suspension stability, redispersibility, wettability and biological activity, and is suitable for being applied by adopting conventional spraying equipment. The insecticidal composition is suitable for preventing and treating various piercing-sucking mouthpart pests, such as aphids, plant hoppers, phylloxera and aleyrodid, and underground pests, such as grub cockroaches and wireworms. The composition can be widely applied to various crops such as rice, cotton, vegetables, fruit trees and tea trees.

This invention provides the application of the citrus psyllid salivary gland gene DcPS2 in the control of citrus psyllids and Huanglongbing (HLB). The nucleotide sequence of the citrus psyllid salivary gland gene DcPS2 is shown in SEQ ID NO. 1. This invention introduces dsDcPS2 into psyllids and measures the effect of DcPS2 gene silencing on psyllid survival. Experimental results show that after DcPS2 gene silencing, the mortality rate of citrus psyllids significantly increased compared to the control dsGFP, which has significant value for the control of citrus psyllids and the cultivation of psyllid-resistant plants.

The invention belongs to the technical field of biological control of agricultural pests, and discloses a method for controlling diaphorina citri through beauveria bassiana based on efficient targeting spore powder and application of the method, and the method comprises the steps that the efficient targeting spore powder is prepared, the collection number of a beauveria bassiana strain is CGMCC No.40957, the spore content is larger than or equal to 10 billion / g, the viable spore rate is larger than or equal to 90%, the beauveria bassiana strain is mixed with a functional auxiliary agent according to the weight ratio of 100: (1-5), and the efficient targeting spore powder is prepared; the functional additive comprises a biochemical permeation promoter, an environment shielding and targeting adhesive and an insect behavior synergist; applying the spore powder to the diaphorina inhabitation area of the citrus plant in a targeted manner by using an electrostatic powder spraying device; and arranging a light insect trapping plate to induce the phylloxera to be in contact with the spore powder. According to the method, the pathogenicity and stress resistance of the beauveria bassiana conidial powder are improved by adding the permeation promoter and the protective agent, and the electrostatic powder spraying and light inducing technologies are combined to realize precise targeting and efficient prevention and treatment of diaphorina citri and reduce the use of chemical pesticides.

The invention discloses a small molecule RNA (Ribonucleic Acid) and application thereof in preventing and treating diaphorina citri. The nucleotide sequence of the micromolecule RNA miR-176 provided by the invention is 5 '-UGACGUGAUGAGAUUUAUGUG-3', and the micromolecule RNA miR-176 is highly expressed in the midgut and ovary of diaphorina citri. By feeding the miR-176 agonist, the diaphorina citri is shortened before oviposition, the oviposition amount is remarkably reduced, the ovary development is abnormal, the titer of the Candidatus Liberibacter asiaticum in the ovary is remarkably reduced, and the miR-176 has a good application prospect in prevention and treatment of female diaphorina citri and prevention of the Candidatus Liberibacter asiaticum.

This invention provides a citrus psyllid salivary gland gene, DcPS4, and its application in the control of citrus psyllids. The nucleotide sequence of the citrus psyllid salivary gland gene, DcPS4, is shown in SEQ ID NO. 1. This invention introduces dsDcPS4 into psyllids and measures the effect of DcPS4 gene silencing on psyllid survival. Experimental results show that after DcPS4 gene silencing, the mortality rate of citrus psyllids significantly increased compared to the control dsGFP, which has significant value for the control of citrus psyllids and the cultivation of psyllid-resistant plants.

The invention discloses a Candidatus Liberobacter asiaticum morbidity prediction method and system based on multi-source data fusion, and relates to the technical field of agricultural disease and pest monitoring, and the method comprises the steps: obtaining the morbidity of the last growth year and day-by-day meteorological data, and completing unit matching; according to a spring shoot period, a summer shoot period, an autumn shoot period and a late autumn shoot period, phenological time windows are divided, and meteorological sequences are aligned; phenological characteristics are extracted in the corresponding time windows; setting the morbidity of the last year as an overwintering medium cardinal number agent and incorporating the morbidity into features; establishing a diaphorina citri ecological mechanism sub-model and calculating a medium population pressure index; fusing the features, the agents and the indexes to construct a morbidity prediction model, and completing regional parameter determination; and generating features and indexes according to the target annual observation or forecast data, and outputting a prediction result. Through phenological segmentation and medium mechanism coupling, proxy variables are introduced and regionalization parameter determination is carried out so that meteorological, medium-to-disease full-link quantification and early warning are realized and prediction accuracy, interpretability and cross-regional applicability are increased.

The application provides a control method for boxwood wood lice by combining nitrogen regulation and visual-olfactory signals, and belongs to the technical field of agricultural ecological control and plant protection. ‑ By controlling the nitrogen application amount to 100-150 kgN per mu , the main peak of the reflectance spectrum of the boxwood leaves is blue-shifted to 510-520 nm, and the volatilization amount of alpha ionone and beta ionone is reduced, so that the visual and olfactory tropism of the wood lice is weakened; meanwhile, a visual-olfactory dual-mode trapping device is arranged above the boxwood canopy, the device is provided with an odor luring core and a color plate with a specific reflection peak (530-560 nm), the dual signals are cooperatively trapped, and in addition, the nitrogen application amount is accurately regulated based on real-time monitoring of the reflectance spectrum, volatiles and wood lice density. Through the method, the wood lice feeding density in the field is greatly reduced, and the amount of chemical pesticides can be reduced by more than 40%.

This invention discloses RanGAP1 Genes and their application in regulating the reproduction of female citrus psyllids. DcRanGAP1 The nucleotide sequence of the gene is shown in SEQ ID NO.9. The female citrus psyllid, eight days after emergence, showed a downregulation. DcRanGAP1 After gene expression, the pre-oviposition period is significantly prolonged, the oviposition period is significantly shortened, the number of eggs laid is significantly reduced, and ovarian development is significantly inhibited and the Huanglongbing titer in the ovary is significantly reduced, thereby achieving the goal of controlling citrus psyllids and providing a new approach to the prevention and control of Huanglongbing.

This invention provides a method for controlling the olive psyllid, belonging to the field of olive cultivation technology. The method includes the following steps: S1: Applying organic fertilizer, superphosphate compound fertilizer, urea, and ammonium bicarbonate to the soil to promote the growth of new shoots and leaves; S2: Collecting adult psyllids, eggs, larvae, or pupae of natural enemies of the psyllid manually and releasing them to fully utilize natural control; simultaneously, spraying with highly effective and low-toxicity pesticides that have low toxicity to natural enemies; S3: Checking for pests each time new shoots emerge and spraying with pesticides, namely acetamiprid wettable powder, milbemycin, and spirotetramat; S4: Winter orchard cleanup. This invention, by spraying acetamiprid wettable powder, milbemycin, and spirotetramat during the control of the olive psyllid, achieves a synergistic effect, synergistically improving the control efficacy of the olive psyllid.

The invention discloses a DcFoxO gene and application of the DcFoxO gene in prevention and treatment of diaphorina citri. The nucleotide sequence of the DcFoxO is as shown in SEQ ID NO. 1. The invention discloses high expression of DcFoxO of diaphorina citri in midgut of diaphorina citri, and by feeding dsRNA targeting DcFoxO, it is found that the egg laying period of female diaphorina citri is shortened, the egg laying amount is reduced, ovarian development is abnormal, the titer of Candidatus Liberibacter asiaticum is reduced, the prevention and treatment effect on diaphorina citri is remarkable, and the application prospect is good.

The application discloses a wood louse killing device for loquat orchard, and belongs to the technical field of wood louse killing devices, and solves the problem that the killing range of the wood louse is small due to the fixed electric net in the prior art wood louse killing device; the device comprises a device main body, the device main body comprises a fixing box, at least one insect inlet is formed in the outer wall of the fixing box; a trapping assembly for attracting wood louses is arranged in the fixing box, a rotating rod is rotatably arranged on the lower surface of the top plate of the fixing box, an electric net assembly is fixed on the rotating rod, and the rotating rod drives the electric net assembly to rotate around the trapping assembly. In the scheme, the wood louses are attracted into the fixing box through the trapping assembly, the electric net assembly is driven by the rotating rod to rotate around the trapping assembly, so that the electric net assembly can kill the wood louses in the whole annular area in the fixing box, the killing range is improved, the wood louses on the electric net assembly can fall off due to gravity, the wood louses cannot be retained on the electric net assembly, and therefore a cleaning assembly does not need to be additionally arranged.

The invention provides a method for preventing and controlling phylloxera in combination with nitrogen regulation and visual-olfactory signals, and belongs to the technical field of agricultural ecological prevention and control and plant protection. According to the method, the nitrogen application amount is controlled to be 100-150kgN. Mu <->, so that the main peak blue of the reflection spectrum of the Chinese wolfberry leaves is shifted to 510-520nm, and the volatilization amount of alpha-ionone and beta-ionone is reduced, so that the visual and olfactory taxis of phylloxera is weakened; meanwhile, a visual and olfactory dual-mode trapping device is arranged above the lycium barbarum canopy, the device is provided with an odor trapping core and a color trapping plate with a specific reflection peak (530-560 nm), dual-signal collaborative trapping is achieved, and in addition, the nitrogen application amount is accurately regulated and controlled based on real-time monitoring of the reflection spectrum, volatile matter and phylloxera density. By means of the method, the feeding density of field phylloxera can be greatly reduced, and the use amount of chemical pesticides can be reduced by 40% or above.

The invention discloses an Alternania iridiaustrialis JY-4 strain and an application of the Alternania iridiaustrialis JY-4 strain in prevention and treatment of Kallkala pomelo, and belongs to the technical field of microbial prevention and treatment of plant insect pests. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 11, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.42162. According to the invention, an entomogenous fungus separated from a stiff aphid body is comprehensively judged through indoor toxicity determination and field control effect tests, and the alternaria alternata JY-4 has a control effect on the adult Kallkala and provides a theoretical support for comprehensive treatment and scientific prevention and control of Kallkala.

The invention belongs to the technical field of biological control, and particularly relates to bacillus amyloliquefaciens and application thereof in pest control. The technical problem to be solved by the invention is to develop new application of bacillus amyloliquefaciens in the field of plant disease control. According to the technical scheme of the invention, the bacillus amyloliquefaciens SCAU-JM13-2 is adopted, and the preservation number of the bacillus amyloliquefaciens SCAU-JM13-2 is GDMCC (China General Microbiological Culture Collection Center) No: 67158. The strain has prevention and treatment effects on diaphorina citri, bean aphids, bemisia tabaci, citrus canker and the like.

The application belongs to the technical field of citrus psylla control, and particularly relates to a high-efficiency citrus psylla control method. The high-efficiency citrus psylla control method comprises the following steps: during the cultivation and growth of citrus, when citrus psylla appears on the citrus, a synergistic insecticide is sprayed, wherein the synergistic insecticide is prepared by compounding cycloprothrin, sulfluramid, spirodien, or ethyl spinosyn as active ingredients, and by adding auxiliary ingredients. In the high-efficiency citrus psylla control method, the active ingredients of the synergistic insecticide sprayed are compounded by cycloprothrin, sulfluramid, spirodien, or ethyl spinosyn. Through indoor joint action tests, it is found that the compounded active ingredients show a joint synergistic effect on killing citrus psylla pests within a certain mass ratio range, can improve the control effect on citrus psylla, and can greatly block the occurrence and spread of citrus Huanglongbing, thereby promoting the healthy development of the citrus industry.

The application discloses DcEZH2 The application discloses a gene and a dsRNA for preventing and treating citrus psylla. The application first clones a citrus psylla DcEZH2 The nucleotide sequence of the gene is shown as SEQ ID NO. 1, and a dsDcEZH2 with a nucleotide sequence shown as SEQ ID NO. 2 is synthesized based on the sequence; the dsDcEZH2 is introduced into the citrus psylla through a feeding method, can efficiently silence the expression of the DcEZH2 gene, thereby causing the mortality of the citrus psylla to be significantly increased, the oviposition amount to be significantly decreased, and the ovary development to be inhibited. The application shows that the decrease DcEZH2 The expression can be used for preventing and treating the citrus psylla, and the provided dsDcEZH2 can be used for preparing a biological pesticide for preventing and treating the citrus psylla, and is suitable for green prevention and control of insect vectors of citrus Huanglongbing.

The invention discloses an insect-derived antibacterial protein. The amino acid sequence of the insect-derived antibacterial protein is shown as SED NO.1. Relates to the technical field of prevention and control of plant diseases and insect pests, and further discloses application of the insect-source antibacterial protein in prevention and control of Candidatus Liberobacter asiaticum. Research finds that in-vivo screening of diaphorina citri which does not carry germs after continuous feeding of plants infected with Candidatus Liberobacter asiaticum finds that a Waprin gene is remarkably up-regulated; protein coded and translated by the gene has a remarkable splitting effect on Candidatus Liberibacter asiaticum. The invention also finds that the insect-source antibacterial protein directly acts on the pathogenic bacteria or the disease-transmitting mediator of the Candidatus Liberobacter asiaticum on the living body level, avoids the ecological risk of chemical agents and the physical control blind area of the pathogenic bacteria, and also makes up the defect that the mediator insect-pathogenic bacteria interaction mechanism cannot be deeply explored depending on natural-source active substances due to the limitation of research materials in the prior art. Further, the blank of efficient targeted prevention and control means is developed, and a new path is provided for green prevention and control and fundamental research breakthrough of the citrus huanglongbing.

The invention discloses a Fusarium parceramosum JY-12 strain and an application of the Fusarium parceramosum JY-12 strain in prevention and treatment of halamias halamiana, and belongs to the technical field of microbial prevention and treatment of plant insect pests. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 11, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.42161. According to the fusarium JY-12 disclosed by the invention, an entomogenous fungus separated from a stiff aphid body is comprehensively judged through indoor toxicity determination and field control effect tests, and the fusarium JY-12 has a control effect on the adult Kallka pomelo, so that a theoretical support is provided for comprehensive treatment and scientific prevention and control of Kallka pomelo.