717 results about "Citrus volkameriana" patented technology

The invention provides bacillus methylotrophicus with preservation number-CCTCC NO. M2013500. The invention also provides a microorganism bacterium agent. The microorganism bacterium agent contains a culture medium and thallus, wherein the thallus comprise the bacillus methylotrophicus with preservation number-CCTCC NO. M2013500, bacillus subtilis, bacillus lincheniformis and bacillus amyloliquefaciens. The invention also provides an application of the bacillus methylotrophicus and the above microorganism bacterium agents to improvement of saline lands, improvement of plant drought tolerance, prevention of bacterial wilt of pepper and prevention of Citrus anthracnose.

Disclosed is a high-efficiency lycoris and citrus interplanting method. The high-efficiency lycoris and citrus interplanting method includes interplanting lycoris under fruit trees such as citrus, covering the lycoris by rotten animal manure straws, managing by processes of fertilizing, weeding, insecticiding and the like, and harvesting after five-year planting. By the high-efficiency lycoris and citrus interplanting method, two kinds of economic plants can be produced on the same field, multiple cropping index is increased, labor cost is saved, production cost is reduced, yield is increased, and economic benefits are doubly increased.

The invention discloses a method for producing bacterial cellulose by taking citrus pomace as a raw material, which belongs to the technical field of microbial fermentation and production. The method comprises the following steps: (1) pretreatment of the citrus pomace; (2) yeast fermentation of pretreatment liquid of the citrus pomace; (3) preparation of production and fermentation culture liquid; and (4) production and treatment of the bacterial cellulose, and the like. The citrus pomace is taken as the raw material, intermediate gluconacetobacter CIs26 CGMCC No.4663 which is autonomously selected and bred, the two-stage type fermentation process is adopted for producing the bacterial cellulose, the yield is as high as 13.5g/L, the production cycle is shortened from the conventional about half a month to 5-10 days, a product is semitransparent and good in flexibility, and the single situation of taking coconut milk as the raw material in the past is overcome. The method can be popularized and applied in bacterial cellulose production enterprises.

The invention provides a preparation for orange bud picking and tip control. The preparation consists of the following components in part by weight: 1 to 20 parts of lactofen, 20 to 40 parts of solvent 1 xylene, 10 to 20 parts of solvent 2 propyl alcohol and/or benzene, and 30 to 60 parts of synergist one or any combination of more of triton X-100, lignosulfonate, agricultural organic silicon surfactant, calcium dodecylbenzene sulfonate, alkylphenol ethoxylate and octachlorodipropyl ether. The invention also provides a preparation method and a using method of the preparation. The preparation has good effect of killing orange buds and delicate tips; the death rate of the buds is 100 percent, the death rate of the delicate tips and half-mature new tips is over 95 percent, and the yield of the orange fruits is increased by 18.2 to 52.3 percent; moreover, the preparation does not have any side effect on other tissues or organs such as the orange fruits, mature branches and leaves, and thelike, and is convenient to use, and the work efficiency is improved by more than 15 times.

The invention specifically discloses a method for preventing and treating candidatus liberibacter asiaticus by heat treatment. The method comprises the specific steps as follows: a citrus tree body infected with candidatus liberibacter asiaticus is subjected to continuous heat treatment for 2-5 hours in the environment at the temperature of 45 DEG C-52 DEG C; then the tree body is cultivated at the environment temperature; and the operation is repeated in the next week, and continuous treatment is performed for 3-4 weeks. The method is economical and environment-friendly, the content of pathogenic bacteria in the infected tree can be greatly reduced or eliminated, the tree vigor of the experimental tree is recovered, the tree grows normally, and production and yield of citrus are obviously improved.

The invention belongs to the field of citrus genomics and discloses a co-dominance InDel molecular marker for identifying single embryo and multiple embryos of citrus and application thereof. By carrying out depth re-sequencing on whole genome DNA of 107 materials including tangelos and oranges, whole genome sequence information is obtained, by means of comparing and screening, the gene sequence information in single-embryo and multi-embryo candidate areas is obtained, the sequence with the highest relevance degree to phenotype is screened out, and the sequence is an InDel sequence of 6bp. Accordingly, a set of simple, fast and co-dominant PCR markers are developed, and primers are used for carrying out PCR amplification on CK F/R, INS F/R and DEL F/R, so that the co-dominance InDel molecular marker for identifying the single embryo and the multiple embryos of the citrus is obtained. Finally, the marker is utilized for testing loci in 115 single-plant natural groups and 100 single-plant hybridized groups. The marker has the biggest advantages that cost is low, accuracy is high, and embryonic early selection can be achieved during the citrus seedling stage.

The invention belongs to the plant protection subject of the agricultural category, and particularly relates to citrus yellow shoot candidatus liberibacter asiaticus detection test paper. The detection test paper is prepared through the following steps that pathogenic bacteria antigen is extracted and purifed from catharanthus roseus host with citrus yellow shoot candidatus liberibacter asiaticus antigen and used for injecting immunized rabbits to obtain yellow shoot candidatus liberibacter asiaticus specific antiserum, the yellow shoot candidatus liberibacter asiaticus specific antiserum is purified to obtain the citrus yellow shoot candidatus liberibacter asiaticus antigen, immune colloidal gold is prepared, a combined pad which is soaked with the immune colloidal gold and a chromatography membrane which is coated with a detection line T-citrus yellow shoot candidatus liberibacter asiaticus antigen and a quality control line C-goat anti-rabbit antibody are further prepared, and finally a detection test paper product is assembled. Against for the requirement of the detection of a great amount of citrus yellow shoot samples, the citrus yellow shoot candidatus liberibacter asiaticus detection test paper which is convenient, rapid and high in specificity is provided. The test paper has following advantages that 1, convenience in use and simpleness in operation are realized; 2, a detection result can be obtained within a short period of time; 3, the stability is good; and 4, the cost is low, and the test paper is particularly suitable for the discrimination of citrus yellow shoot plants of a fruit garden.

The invention relates to a citrus breed standard DNA fingerprint spectrum library which is obtained according to the steps of extracting total DNA of various breeds of citrus, screening sequences of SSR (Simple Sequence Repeats) characteristic primer pairs, obtaining a citrus DNA characteristic fingerprint spectrum and constructing a citrus standard DNA fingerprint spectrum library. The citrus breed standard DNA fingerprint spectrum library has the advantages of good repeatability, stability and reliability and rapid detection when applied to distinguishing the citrus breeds, provides the basis to distinguish the citrus breeds by using an SSR molecule marker technology, can be directly applied to the true and false distinguishing of the citrus breeds, solves the difficulty of early distinguishing for the citrus nursery stock for a long term, and ensures that the citrus breeds are not limited by space-time conditions.

The invention provides a citrus fruit wine brewing saccharomycete with a conservation number of CCTCCNO: 2012472, and a production method of using the saccharomycetes to brew high-quality citrus fruit wine. The method comprises the following steps: raw material processing, juice clarifying, pectolase processing, fermentation seed preparation, primary fermenting, filtering and slag removing to obtain a raw wine liquid, fermenting at the 2 DEG C, and storing for 2 months to get the finished product of the citrus fruit wine. The citrus fruit wine has a straw colour, is clear, transparent and glossy, has pure and unique aroma of citrus fruit, has refreshing and delicate taste, is full-bodied and harmonic, has lingering finish and also has the typical characteristics of the citrus fruit wine. According to the invention, the way to wealth for fruit grower is opened in the aspect of deep processing of citrus.

The invention relates to the technical field of pesticides, and particularly relates to a bactericide composition which takes zhongshengrnycin and copper oxychloride as effective ingredients, has an obvious and enlarged bacteriocidal spectrum and no phytotoxicity, and is used for treating plant bacterial diseases as well as fungal diseases. The weight proportion of the zhongshengrnycin to the copper oxychloride is 1:5-60. The bactericide composition has obvious synergistic effect, high field prevention effect and a wide bacteriocidal spectrum, is used for preventing and treating cucumber bacterial angular leaf spot, cucumber downy mildew, tomato bacterial macula disease, tomato leaf mold, pumpkin soft rot, watermelon bacterial angular leaf spot, watermelon sponge, apple alternaria leaf spot, apple brown blotch, pear scabs, grape downy mildew, grape powdery mildew, anthracnose of grape, jujube rust diseases, jujube anthracnose, citrus canker, citrus scab, citrus anthracnose, peanut leaf spot diseases and peronophythora litchii, and has the advantages of good effect and reduction of prevention and treatment cost.

The invention relates to the technical field of orange insect disease prevention and treatment and discloses a composition for trapping diaphorina citri and a trapping method using the same. The composition comprises gamma-terpinene, linalool, beta-caryophyllene and alpha-copaene. The trapping method comprises the following steps of putting the composition into a transparent plastic insect-trapping bottle, covering the bottom with a bottle cover, spraying an insect binder to the outer part of the insect-trapping bottle to obtain an insect binding bottle, hanging the insect binding bottle on a diaphorina citri host plant, and carrying out diaphorina citri trapping and binding. The composition can improve a trapping amount by 62 times in the case of high diaphorina citri density, has substantial trapping effects, can be used for preventing, treating and forecasting diaphorina citri, and can also be used for preventing citrus Huanglongbing propagated by diaphorina citri.

The invention relates to the technical field of agricultural planting, in particular to a composite microbial agent for citrus tristeza virus, a preparation method thereof and a special biological agent for the citrus tristeza virus prepared by using the same. According to the invention, bacillus thuringiensis, beauveria bassiana and trichoderma harzianum are used to control the pathogen of citrustristeza virus pathogens on the ground and kill a spreading source wood louses and eggs thereof so as to effectively control the occurrence of the citrus tristeza virus, and four kinds of microorganism bacteria underground can effectively control the invasion of pathogenic microorganisms, effectively control the spread of pathogenic bacteria by nematodes, and simultaneously regulate the soil structure, decompose organic matters and improve the soil fertility. By promoting the growth of citrus roots with citrus specific AM fungi, citrus can grow new roots in clusters, the number of the new roots is enlarged, the tree vigor is enhanced, the immunity and disease resistance of trees are improved, a nitrogen fixation system is formed, and the pathogen control is combined with the improvement of the ecological environment for the growth of the citrus to achieve the effect of preventing and treating the citrus tristeza virus.

The invention provides highly sensitive and specific assays for the major citrus pathogens Xylella fastidiosa and Xanthomonas axonopodis, including a field deployable multiplexed assay capable of rapidly assaying for both pathogens simultaneously. The assays are directed at particular gene targets derived from pathogenic strains that specifically cause the major citrus diseases of citrus variegated chlorosis (Xylella fastidiosa 9a5c) and citrus canker (Xanthomonas axonopodis pv citri). The citrus pathogen assays of the invention couple a highly efficient isothermal amplification technique with lateral flow nucleic acid hybridization detection, thereby providing an inexpensive and facile means of rapidly detecting pathogen nucleic acid targets while circumventing hardware requirements for fluorescence detection and PCR thermocycling. The citrus pathogen assays of the invention offer femtomole sensitivity, excellent linear dynamic range, and rapid and specific detection.

The invention belongs to the field of plant new species breeding technique, specifically to a method of transferring citrus cytoplasm male sterility trait, which is characterized in that based on the phenomenon that the citrus cell symmetrical amalgamation can regenerate diploid diachyma parent-type cybrid and its constituting features at molecule level, the cytoplasm male sterility trait controlled by the mitochondrial genome is transferred by amalgamation compounding matching and the cybrid obtained by culturing, thereby improving the diploid nuclear or multi-core citrus breeds. The invention discloses the symmetric amalgamation between cytoplasm male sterility Wenzhou Satsuma and Chinese characteristic nuclear or multi-core citrus, successfully invents a batch of diploid cybrids, realizes the effective transfer of Wenzhou Satsuma mitochondrial genome, and provides a novel method and new germplasm materials for diploid Satsuma breeding.

The invention discloses an intelligent citrus disease and pest diagnosis method and system based on deep learning. The intelligent citrus disease and pest diagnosis method comprises the steps: 1, establishing an image data set of six citrus diseases including Huanglongbing, anthracnose, canker, black spot, sand paper rust and scabis based on expert experience; 2, expanding the training set and the test set by using five data enhancement methods; using the enhanced training set and verification set to train the simplified DenseNet network, and storing the model in the system; evaluating the performance of the model by using the test set; and 3, establishing a citrus disease diagnosis system on the basis of the WeChat applet, photographing/uploading images by a user through a mobile phone by using the applet, performing diagnosis through the uploaded trained convolutional network model, and returning an intelligent diagnosis result and pest control suggestions to the user to realize intelligent diagnosis of citrus diseases and pests.

The invention discloses a bactericidal composition, a pesticide and application thereof. The bactericidal composition comprises the following components of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole-5-formamide and cuppric nonyl phenolsulfonate in a weight ratio of (1:50)-(50:1). The bactericidal composition is used as an active component and combined with agromedically acceptable auxiliary components together so as to form a pesticide with a sterilization effect; the pesticide can effectively prevent crop diseases, such as rice bacterial leaf blight, citrus canker, pseudomonas solanacearum, tobaccomosaivirus, pepper virus disease, tomato virus disease and the like; the effect of the pesticide is obviously higher than that of a double-component single agent; and a remarkable synergetic effect is achieved, the application dosage of each single agent can be effectively reduced, the better effects of enlarging sterilization scope and delaying plant pesticide-resistance are achieved, the duration is prolonged, and a remarkable promotional value is obtained.

The invention discloses a split-bin-type anti-aging cosmetic composition, and relates to the technical field of cosmetics. The split-bin-type anti-aging cosmetic composition comprises a composition Aand a composition B; the composition A is an oily liquid and is independently sealed; the composition B is an aqueous liquid and is independently sealed; the composition A contains retinyl palmitate,coated retinol, tocopherol and compound vegetable oil; and the composition B contains nicotinamide, a citrus peel extract, soluble proteoglycan, a myrothamnus flabellifolia leaf/stem extract, a hylocereus undatus fruit extract, sodium hyaluronate, coated ferulic acid, carnosine and 3-o-ethyl ascorbic acid. According to the split-bin-type anti-aging cosmetic composition provided by the invention, the substances in the composition A and the composition B are compounded according to a proper ratio and have synergistic interaction, so that the cosmetic composition has the anti-oxidation and anti-aging effects of improving skin elasticity, improving skin smoothness and reducing skin wrinkles.

The invention discloses Saccharomyces cerevisiae BY23, with broad bacteriostasis spectrum and stable effect, for controlling postharvest diseases of fruits and a use method and use thereof. The strainhas a collection strain serial number of CGMCC No. 14905 in General Microorganisms Center of China Committee for Culture Collection of Microorganisms. The use method of the saccharomyces cerevisiae comprises the steps: activating the strain, carrying out fermentation culture with YPD, carrying out centrifugation, and preparing a 1*10<8>cell/mL bacterial suspension from thalli and sterile water; placing the fruits such as apples, pears, grapes or oranges into the bacterial suspension, carrying out soaking for 30 seconds, then, taking out the fruits, and drying the fruits in the air; and placing the fruits into a fresh-keeping box, and carrying out storage at normal temperature. The saccharomyces cerevisiae strain can be used for simultaneously controlling blue molds, Botrytis cinerea and black spot of apples, Botrytis cinerea of pears, black spot, Fusarium molds, anthrax and Phoma glomerata of grapes and blue molds of oranges, and loss caused by the postharvest diseases is reduced, sothat the saccharomyces cerevisiae strain has a very good application prospect.

The invention discloses a method for rapidly calculating citrus yield through pictures. The method comprises the following steps that S1, an intelligent terminal obtains a single citrus picture; S2, recognizing the single-plant citrus picture obtained in the step S1 through a network model, and returning a single-plant recognized fruit grain number n, a k (m) tree age coefficient and a k (w) treeform coefficient; S3, obtaining the citrus number through the following formula: P = n * [e [k (n) + k (m) + k (w)]- 1}]*[ln(x+1)]*y*z; Wherein P is the citrus yield, n is the number of fruit grains identified by a single plant, k (n) is the fruit grain coefficient, k (m) is the tree age coefficient, k (w) is the tree coefficient, x is the average single grain weight, y is the average plant numberper mu, and z is the number per mu. According to the method, pictures of single citrus trees in the fruiting stage are shot through the intelligent terminal, the number of citrus fruits is intelligently recognized, the fruit grains of the single citrus trees can be scientifically estimated according to the fruit grain coefficient, the tree age, the tree shape and the weight characteristics of thesingle citrus fruits, and the citrus yield in the planting area is accurately calculated.

The invention relates to the technical field of aquatic product culture, in particular to a method for inhibiting iridovirus proliferation in a micropterus salmoides culture process of an RAS system.The method comprises the step of adding citrus flavone extract and black Chinese wolfberry anthocyanin extract into the RAS system. According to the method, the citrus flavone extract and the black Chinese wolfberry anthocyanin extract have a synergistic effect, so that the proliferation of iridovirus is inhibited, and the technical problem that the iridovirus in an RAS culture system causes fishto die due to illness is solved. By implementing the scheme, cultured fishes can be prevented from being infected by iridovirus, the survival rate of aquatic products is increased, the quality of theaquatic products is improved, and economic losses are reduced.

The invention discloses Saccharomyces ellipsoideus BY36, with broad bacteriostasis spectrum and stable effect, for preventing and treating postharvest diseases of fruits and vegetables and a use method and use thereof. The strain has a collection strain serial number of CGMCC No. 14910 in General Microorganisms Center of China Committee for Culture Collection of Microorganisms. The use method of the Saccharomyces ellipsoideus comprises the steps: activating the strain, carrying out fermentation culture with YPD, carrying out centrifugation, and preparing a 1*10<8>cell/mL bacterial suspension from thalli and sterile water; placing the fruits and vegetables such as apples, pears, grapes, strawberries, oranges or cherry tomatoes into the bacterial suspension, carrying out soaking for 30 seconds, then, taking out the fruits and vegetables, and drying the fruits and vegetables in the air; and placing the fruits and vegetables into a fresh-keeping box, and carrying out storage at normal temperature. The Saccharomyces ellipsoideus strain can be used for simultaneously controlling blue molds and gray molds of apples, gray molds of pears, gray molds, aspergillosis, black spots, anthrax andpink rot of grapes, gray molds of strawberries, blue molds of oranges and gray molds and aspergillosis of cherry tomatoes, and loss caused by the postharvest diseases is reduced, so that the Saccharomyces ellipsoideus strain has a very good application prospect.