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Saccharomyces cerevisiae BY23 for controlling postharvest diseases of fruits and preparation and use methods thereof

A technology of fruit postharvest disease and Saccharomyces cerevisiae, which is applied in the field of biological control of fruit postharvest disease, can solve the problems that the biocontrol effect is only verified on a small number of fruits, and the lack of antibacterial spectrum strains, etc., achieves significant social and ecological benefits, Stable properties and high safety effects

Active Publication Date: 2018-04-06
北京华康信使科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although there are nearly a hundred species of antagonistic yeasts reported at home and abroad, the biocontrol effects of most antagonistic yeasts have only been verified on a few fruits.
However, since the biocontrol effects of different strains of the same yeast are very different (Filonow et al., 1996), most of the antagonistic yeasts lack strains with broad antibacterial spectrum and stable effect.

Method used

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  • Saccharomyces cerevisiae BY23 for controlling postharvest diseases of fruits and preparation and use methods thereof
  • Saccharomyces cerevisiae BY23 for controlling postharvest diseases of fruits and preparation and use methods thereof
  • Saccharomyces cerevisiae BY23 for controlling postharvest diseases of fruits and preparation and use methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Biological properties of Saccharomyces cerevisiae BY23

[0016] 1. Morphological characteristics

[0017] (1) YPDA medium (1% yeast extract powder, 2% peptone, 2% glucose, 1.8% agar, sterilized at 121°C for 20 minutes) was cultured at 26°C for 48h, and the colonies were round and white with smooth and round edges. The cell shape is ellipsoidal.

[0018] (2) After culturing in YPDA liquid medium for 24 hours, no mold was formed, the bacterial solution was turbid, and there was precipitation. Microscopically, the yeast cells were oval and budded.

[0019] 2. Molecular biological identification

[0020] The general forward primer NL-1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and the reverse primer NL-4 (5'-GGTCCGTGTTTCAAGACGG-3') were used to amplify the nucleic acid sequence of Saccharomyces cerevisiae BY23 26S rDNA D1 / D2 region by PCR. The sequencing results of the PCR products were entered into the website www.NCBI.nlm.nih.gov for BLAST, the homologous sequences w...

Embodiment 2

[0022] The inhibitory effect of implementation example 2 Saccharomyces cerevisiae BY23 on apple penicillium and botrytis cinerea

[0023] 1. Experimental protocol

[0024] Take Saccharomyces cerevisiae BY23 out of the -80°C refrigerator, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and pick a single colony Put it into YPD liquid medium, cultivate it under the conditions of 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on the hemocytometer and prepare a concentration of 1×10 8 cells / mL of Saccharomyces cerevisiae BY23 suspension.

[0025] Activate Penicillium expansum, Botrytis cinerea or Alternaria tenuissima on PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, wash with sterile water Prepared to a...

Embodiment 3

[0035] Implementation example 3 Saccharomyces cerevisiae BY23 is to the inhibitory effect of pear fruit gray mold

[0036] 1. Experimental protocol

[0037] Take Saccharomyces cerevisiae BY23 out of the -80°C refrigerator, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and pick a single colony Put it into YPD liquid medium, cultivate it under the conditions of 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on the hemocytometer and prepare a concentration of 1×10 8 cells / mL of Saccharomyces cerevisiae BY23 suspension.

[0038] Activate Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Botrytis cinerea spore susp...

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Abstract

The invention discloses Saccharomyces cerevisiae BY23, with broad bacteriostasis spectrum and stable effect, for controlling postharvest diseases of fruits and a use method and use thereof. The strainhas a collection strain serial number of CGMCC No. 14905 in General Microorganisms Center of China Committee for Culture Collection of Microorganisms. The use method of the saccharomyces cerevisiae comprises the steps: activating the strain, carrying out fermentation culture with YPD, carrying out centrifugation, and preparing a 1*10<8>cell / mL bacterial suspension from thalli and sterile water; placing the fruits such as apples, pears, grapes or oranges into the bacterial suspension, carrying out soaking for 30 seconds, then, taking out the fruits, and drying the fruits in the air; and placing the fruits into a fresh-keeping box, and carrying out storage at normal temperature. The saccharomyces cerevisiae strain can be used for simultaneously controlling blue molds, Botrytis cinerea and black spot of apples, Botrytis cinerea of pears, black spot, Fusarium molds, anthrax and Phoma glomerata of grapes and blue molds of oranges, and loss caused by the postharvest diseases is reduced, sothat the saccharomyces cerevisiae strain has a very good application prospect.

Description

technical field [0001] The invention relates to the field of biological control of postharvest diseases of fruits, in particular to a strain of Saccharomyces cerevisiae used for biological control of postharvest diseases of fruits. Control effect. Background technique [0002] Although the deterioration of fresh fruit quality is affected by many factors, disease is the main reason. Among them, rot and deterioration caused by fungal diseases is the most serious factor in postharvest fruit loss. Although postharvest diseases of fruits can be prevented and controlled in many ways such as agricultural control, physical control, chemical control and biological control, the main measure at present is chemical control (Eckert & Ogawa, 1985, 1988). However, long-term use of chemical pesticides not only leads to the development of drug resistance of pathogenic bacteria but also reduces the bactericidal effect (Prusky et al., 1985; as et al., 1991; Holmes&Eckert, 1999), and the fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23B7/155A01N63/04A01P3/00C12R1/865
CPCA23B7/155A23V2002/00A01N63/30C12N1/16C12N1/185C12R2001/865A23V2250/762
Inventor 王友升姚婷李丽萍任向峰黄津津王颖
Owner 北京华康信使科技有限公司
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