The invention discloses a marine bios sterilization solution. The effective sterilization ingredients of the sterilization solution are prepared from beta-chitosan quaternized hyaluronic acid derivative, algal polysaccharide derivative, alpha-spiral cecropin serving as a marine organism, biological enzyme and cationic polyguanidine by a special technology. The sterilization solution is suitable for sterilization of restaurants, hotels, hospitals, marketplaces, banks, schools, kindergartens, households, offices and journey, can be used as a remedial measure after hand washing in hospitals, nursing centers, nursery centers, snack bars, kitchens and bathrooms, or can be used for sterilizing skin wounds and cleaning vulva and the like, can effectively control the propagation of typhoid fever, flu, dysentery, pinkeye, gonorrhea and various skin diseases, and has no stimulus or corrosion to the skin. The marine bios sterilization solution is characterized by comprising the following components in percentage by weight: 1.0 to 2.0 percent of beta-chitosan quaternized hyaluronic acid derivative, 0.5 to 1.0 percent of algal polysaccharide derivative, 6.0 to 8.0 percent of alpha-spiral cecropin serving as a marine organism, 0.5 to 1.0 percent of biological enzyme, 3.0 to 4.0 percent of cationic polyguanidine and the balance of deionized water.