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Anti bacteria natural silk composite nano-fiber material and its preparation method

A technology of composite nanofibers and antibacterial silkworms, which is applied in the fields of rayon manufacturing, fiber chemical characteristics, cellulose/protein conjugated artificial filaments, etc. Wound healing and other issues, to achieve excellent bactericidal effect, excellent bactericidal, and maintain the effect of persistence

Inactive Publication Date: 2007-01-31
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also nanofiber non-woven membranes made of silk protein by electrospinning, but they have no antibacterial properties, and the scope of use is limited to a certain extent.
[0004] The Chinese invention patent with the publication number CN1348820A "An Antibacterial Silk Fibroin Wound Protective Film and Its Preparation Method" discloses that silk is used as the main film-forming raw material, antibacterial agents and additives are added, and the salivation method is applied through layered coating and hot air drying. The prepared antibacterial silk fibroin wound protective film is a dense film formed by hot air drying. Although it has a good antibacterial effect, the flexibility of the silk fibroin wound protective film will be affected by hot air drying; Pores affect its water permeability and air permeability, which is not conducive to wound healing, and the effect is affected to a certain extent

Method used

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  • Anti bacteria natural silk composite nano-fiber material and its preparation method
  • Anti bacteria natural silk composite nano-fiber material and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Put 0.1 kg of leftover silk into 3 liters of 0.05% sodium carbonate aqueous solution by mass fraction, boil for 0.5 hour, repeat the treatment three times, remove all the sericin around the silk, and obtain silk fibroin;

[0023] 2. After drying the obtained silk fibroin, use 0.6 liter of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 to heat and dissolve at 78±2°C to form a silk protein solution;

[0024] 3. Pour the above-mentioned silk protein solution into the cellulose semi-permeable membrane, dialyze with tap water first, and then dialyze with deionized water to remove ethanol and calcium chloride small molecules in the solution, and then filter with multi-layer degreasing gauze to prepare Obtain pure silk fibroin solution;

[0025] 4. Take 100ml of pure silk fibroin solution and pour it into an area of ​​20×20cm 2 In a polystyrene (ABS) tray, dry film under 25 ℃, RH65% constant temperature and humidity conditions, take off the regene...

Embodiment 2

[0034] 1. Put 0.2 kg of waste mulberry silk into 6 liters of aqueous sodium carbonate solution with a mass fraction of 0.05%, boil for 0.5 hour, repeat the treatment three times, remove all the sericin around the silk, obtain silk fibroin, and dry at room temperature;

[0035] 2. Dissolve the dried silk fibroin with 1.2 liters of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 at 78±2°C to form a silk protein solution;

[0036] 3. Pour the fibroin solution prepared in step 2 into a cellulose dialysis bag, first dialyze with tap water, then dialyze with deionized water to remove ethanol and calcium chloride in the solution, and then filter with degreasing gauze to obtain Pure silk fibroin solution;

[0037] 4. Pour 100ml of pure silk fibroin protein solution into a stainless steel plate, freeze at -25°C for 12 hours, and then vacuum-dry it in a freeze dryer for 24 hours to obtain a spongy silk fibroin film, which is bagged for use;

[0038] 5. 6.7 gram...

Embodiment 3

[0046] 1. Put 0.2 kg of waste mulberry silk into 6 liters of aqueous sodium carbonate solution with a mass fraction of 0.05%, boil for 0.5 hour, repeat the treatment three times, remove all the sericin around the silk, obtain silk fibroin, and dry at room temperature;

[0047] 2. Dissolve the dried silk fibroin with 1.2 liters of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 at 78±2°C to form a silk protein solution;

[0048] 3. Pour the fibroin solution prepared in step 2 into a cellulose dialysis bag, first dialyze with tap water, then dialyze with deionized water to remove ethanol and calcium chloride in the solution, and then filter with degreasing gauze to obtain Pure silk fibroin solution;

[0049] 4. Pour 100ml of pure silk fibroin solution into a stainless steel plate, freeze at -18°C for 8 hours, and then vacuum-dry it in a freeze dryer for 18 hours to obtain silk fibroin powder, which is bagged for use;

[0050] 5. Join 16.2 grams of silk ...

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Abstract

This invention discloses a compound antibacterial silk nanometer fibrous material, accounting for weight its component proportion are: Sericin protein 58-90%; chitosan or chitin 0-40%, antibacterial agent 0-5%, electric conduction 1-4%,crossing linking 1-4%. Its process is: dissolve the pure sericin protein, antibiotics separately in polar solvent, join electric conduction medicinal preparation and crosslinking agent, using the static electricity spinning technique, making the average aperture for 0.5-5.0 micron, factor of porosity 60-90%, the mean diameter is 40-100nm and the material is nanometer textile fiber adhesive-bonded membrane. This invention has good organization compatibility with human body, Antibiotics and sericin insoluble is in homogeneous solvent, massive holes among the nanometer textile fiber adhesive-bonded membrane, which has outstanding aqueous percolation permeability, antibacterial agent distributes evenly in the nanometer textile fiber, and the medicine release effect is perfect, so it can be a kind of ideal resist bacteria injured medical material.

Description

technical field [0001] The invention relates to a medical biological material and a preparation method thereof, in particular to a nanofiber antibacterial material composed of silk fibroin and an antibacterial agent and a preparation method thereof, belonging to the technical field of polymer materials. Background technique [0002] Before the present invention was made, there were reports on the use of natural or synthetic high polymers as main raw materials to make nanofiber non-woven membranes through electrospinning, such as: raw materials are polyacrylonitrile, polyamide, polyvinyl alcohol, cellulose And its derivatives, etc., but some of the above materials have poor compatibility with the human body, and some spun fibers are relatively thick, with an average diameter of several hundred to several thousand nanometers, which are not suitable for medical materials. [0003] Silk protein is a fairly pure natural protein, which is composed of 18 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F8/02D01F8/18D01F1/10D01D5/06
Inventor 张幼珠尹桂波王立新何莉
Owner SUZHOU UNIV
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