6results about How to "Good biological control effect" patented technology

This invention discloses a method for controlling bacterial wilt and soil antibiotic resistance genes based on a bio-liquid-microorganism-silicon synergy, belonging to the fields of agricultural microbial technology and soil remediation technology. The method includes the following steps: S1, using straw from fields frequently affected by bacterial wilt as raw material, a renewable antibacterial bio-liquid rich in phenolic and ketone compounds is prepared; S2, Bacillus subtilis is subjected to stress-adaptive screening and continuous domestication culture to obtain domesticated strains; S3, the domesticated strains are expanded to prepare a biocontrol agent with enhanced antagonistic ability and environmental tolerance; S4, under pot conditions, the optimal combination of silicon fertilizer, antibacterial bio-liquid, and stress-adaptive biocontrol agent is screened to clarify its role in reducing the spread of antibiotic resistance genes in the soil-plant continuum; S5, under field conditions, silicon fertilizer, antibacterial bio-liquid, and stress-adaptive biocontrol agent are synergistically applied to inhibit soil-borne diseases and simultaneously reduce the spread of antibiotic resistance genes in the soil-plant continuum. This invention has the advantages of stable and sustained control effect and high ecological safety. It can effectively protect the healthy growth of crops and reduce the risk of the spread of resistance genes.

This invention discloses a strain of *Bacillus amyloliquefaciens*, JDF630, and its application in controlling *Fusarium wilt* of *Cotinus coggygria*, belonging to the field of microbial technology. The *Bacillus amyloliquefaciens* strain JDF630 described in this invention was deposited on July 13, 2022, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC NO. 25289. This invention demonstrates that strain JDF630 exhibits broad-spectrum inhibitory activity against various plant pathogens. Treatment with JDF630 significantly reduces the damage caused by *Fusarium wilt* to *Cotinus coggygria*, providing a new microbial strain and control strategy for the prevention and control of plant pathogens, demonstrating excellent biological control effects.

This invention discloses a method for enhancing the ability of Trichoderma to control plant diseases, belonging to the field of biocontrol technology. This invention is the first to identify the rice black spore divirus NoPV1-COTG-19 carried by *Trichoderma guiyuan* COTG-19, and confirms that this virus can enhance the inhibitory ability of *Trichoderma* against plant pathogens. Plate confrontation experiments show that the virus-carrying COTG-19 strain has a significantly higher inhibition rate against *Pseudomonas aeruginosa* than the virus-free COTG-19-FV strain. Pot and field trials show that the NoPV1-COTG-19 strain has excellent control effects against *Tetracentron sinense* canker, and the NoPV1-COTG-19 virus can be horizontally transmitted to other *Trichoderma* strains, significantly enhancing the sporulation capacity of infected *Trichoderma* strains. This invention provides a novel technical approach to improving the biocontrol efficacy of *Trichoderma*, and has broad application prospects in the field of green control of plant diseases.

This invention discloses a biocontrol bacterium for bacterial wilt, YL-Ste-01evo (accession number CCTCC NO: M2022939); a biocontrol bacterium for bacterial wilt, YL-Ste-01 (accession number CCTCC NO: M2022324); and a lytic bacteriophage, YL-Ste-01P (accession number CCTCC NO: M2022460). This invention discloses the application of biocontrol bacterium YL-Ste-01evo, or biocontrol bacterium YL-Ste-01, or biocontrol bacterium YL-Ste-01evo in synergy with bacteriophage YL-Ste-01P in the control of soil-borne bacterial wilt in tomato. The biocontrol bacterium YL-Ste-01 can significantly inhibit the growth of Ralstonia solanacearum and reduce the incidence of bacterial wilt in tomato plants. Through phage stress, YL-Ste-01 evolved into a biocontrol bacterium YL-Ste-01evo with stronger phage resistance and better antibacterial effect.

The present application belongs to the field of microbial technology, and particularly relates to a strain of controvirus VP-1 and a fermentation liquor and application thereof. The present application provides a strain of controvirus VP-1, which has been biologically preserved with a preservation number of CCTCC NO: M20221633. The controvirus VP-1 of the present application can be used for preventing and treating plant diseases and promoting plant growth. The results of examples show that the controvirus VP-1 obtained by the present application can promote the growth of rape roots and has a good biocontrol effect on sclerotinia stem rot of rape, thereby providing a new and beneficial biocontrol resource for biological control of crop fungal diseases.

This invention belongs to the field of microbial technology and discloses a strain of *Bacillus zeylanus* and its application in the control of plant diseases, particularly its application in the control of root rot in wolfberry. The *Bacillus zeylanus* strain (… Paenibacillus zeae This strain, isolated from the rhizosphere soil of wolfberry plants suffering from root rot, is designated R2 and has been preserved under the CGMCC No. 36137. It inhibits the growth of various fungal pathogens, including *Lycium chinense*, the causal agent of wolfberry root rot, produces cellulase and amylase, and can induce plant... PR1 , CAT2 The strain expressed defensive genes. Pot experiments showed that it achieved a 66.66% control effect against wolfberry root rot, significantly reducing the incidence and disease index, and providing an effective biological resource for the green control of plant diseases, especially wolfberry root rot.