25results about How to "Good biological control effect" patented technology

The invention belongs to the technical field of biological prevention and control of postharvest diseases, and particularly relates to biocontrol serratia rubra and application thereof to postharvest disease control, storage and fresh keeping of pear fruits. The method comprises the following steps: activating and culturing serratia rubra, centrifuging after culturing to obtain thalli, and re-suspending by using sterile normal saline to obtain a bacterial suspension; the method comprises the following steps: punching holes in the surfaces of pears, injecting the holes, and then injecting penicillium expansum spore suspension with the same volume, thereby realizing effective control on penicilliosis caused by penicillium expansum; or uniformly spraying the serratia rubra suspension on the surfaces of the pears, and naturally airing, so as to realize the purposes of disease control, storage and fresh-keeping after the pears are picked. According to the method, the occurrence of penicilliosis after the pears are picked can be remarkably reduced, the natural rotting rate after the pears are picked is reduced, the method is safe and environmentally friendly, no remarkable adverse effect is generated on main quality indexes of the picked pears, the storage quality of the pears can be kept, a remarkable technical effect is achieved, and the application prospect is wide.

The invention relates to the technical field of microorganisms, in particular to paenibacillus polymyxa ZR-4 and application of the paenibacillus polymyxa ZR-4, the paenibacillus polymyxa ZR-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is May 17, 2024, and the preservation number is CGMCC No.30589. The paenibacillus polymyxa ZR-4 is named as Paenibacillus polymyxa ZR-4. The paenibacillus polymyxa ZR-4 provided by the invention not only can promote the growth of plants such as strawberries, corns and rice, but also has a biological control effect on agricultural diseases such as strawberry gray mold and corn southern leaf blight, has an excellent antagonistic effect on pathogenic bacteria of various plant diseases, and has a wide application prospect and development space.

The invention relates to the technical field of storage of tobacco powder borers, and particularly discloses a method for killing stored tobacco powder borers by feeding adult cotesia testis in batches, which comprises the following steps of: adding bee sweet water into a feeding box of host larvae of the cotesia testis for feeding; meanwhile, the temperature, the humidity and the bee sugar water liquid level in the feeding box are subjected to time sequence analysis by adopting a data processing technology based on deep learning, so that the time sequence consumption trend of the bee sugar water liquid level and a time sequence correlation mode of the temperature and the humidity in the box are extracted; the dynamic association relationship between bee sugar water consumption and the environmental condition in the box is disclosed by carrying out interactive analysis on the time sequence association characteristics of the temperature and humidity in the box and the time sequence consumption characteristics of the bee sugar water, so that the intelligent recommendation of the bee sugar water flow under the current environmental condition is realized. By means of the mode, optimal control over bee sugar water supply can be achieved, the breeding conditions of the adult cotesia gigas can be improved, and therefore the survival rate of the cotesia gigas and the quality of the adult cotesia gigas are improved, and the biological control effect is enhanced.

The invention belongs to the field of microorganisms, and relates to a fungus F67 and application thereof, the fungus F67 is Lecanicillium saksenae, the fungus F67 is submitted to CGMCC (China General Microbiological Culture Collection Center) on October 9, 2024 and is preserved, and the preservation number is NO.41531. The invention further discloses a preparation method of the fungus F67. The invention provides a fungus F67 capable of effectively constructing a rhizosphere biological barrier system, promoting healthy growth of tobacco plants and providing theoretical support for green prevention and control of tobacco rhizome diseases, and application of the fungus F67.

The invention belongs to the technical field of plant cultivation, and particularly relates to a screening and fermentation method of a pepper root rot antagonistic bacterium W-5, which comprises the following steps: 1, preparing experimental materials, namely preparing a test strain, a culture medium, a reagent and an instrument; 2, separating, screening and identifying the antagonistic bacteria; 3, observing the influence of the antagonistic bacteria W-5 on the growth of the mycelia at the front end of the fusarium solani; step 4, detection of pathogenic fungus bacteriostasis spectrum by antagonistic bacteria W-5, the structure is reasonable, the main pathogenic fungus fusarium solani causing the pepper root rot disease is taken as an indicator bacterium, the pepper root rot antagonistic bacteria are obtained through separation by adopting a soil plate dilution method and a plate confrontation method, and the antagonistic bacteria are identified by combining morphology with molecular biology; meanwhile, the broad-spectrum antibacterial activity of the strain W-5 is measured, and the optimal fermentation condition of the strain W-5 is optimized based on a response surface method designed by combining a single-factor test with Box-Behnken, so that ginseng is expected to be provided for development and application of a pepper root rot antagonistic biological agent.

The invention relates to the technical field of microorganisms, in particular to Pseudomonas S11 and application thereof, the Pseudomonas S11 is preserved in China Center for Type Culture Collection, the preservation address is No. 299, eight road, Wuchang District, Wuhan City, Hubei Province, the preservation date is March 21, 2022, and the preservation number is CCTCC NO: M2022294. The pseudomonas S11 provided by the invention not only can promote the growth of plants such as strawberries, corns and rice, but also has a biological control effect on agricultural diseases such as strawberry gray mold and corn southern leaf blight, has an excellent antagonistic effect on pathogenic bacteria of various plant diseases, and has a wide application prospect and development space.

The invention discloses paecilomyces lilacinus M-2 for degrading fosthiazate in greenhouse vegetable soil and a preparation method of the paecilomyces lilacinus M-2. The bacterial strain is paecilomyces lilacinus M-2, and the preservation number of the bacterial strain is GDMCC No: 65294. The invention further discloses a preparation method of the paecilomyces lilacinus strain. The paecilomyces lilacinus M-2 provided by the invention is domesticated and prepared into the fosthiazate degrading microbial inoculum, and experiments prove that the degrading microbial inoculum has a rapid degradation effect on pesticides in greenhouse vegetable soil polluted by fosthiazate, and meanwhile, the control effect of fosthiazate on root-knot nematode is not influenced; meanwhile, enhancement of drug resistance of pathogenic bacteria caused by the action of chemical pesticides is effectively reduced, and the balance of a soil micro-ecosystem is protected.

The invention relates to the technical field of Siamese bacillus pesticide preparations, and discloses a mineral source-containing Siamese bacillus pesticide preparation, which comprises the following ingredients: a main active component, a mineral source component, a synergist, a carrier material, a stabilizer, an antioxidant, a nutritional supplement, a wetting agent, a dispersing agent, an adhesive, a film-forming agent, a pH regulator, a filler and water. According to the siamensis siamensis pesticide preparation containing the mineral source, by using the pH buffering agent and the stabilizing agent, it is ensured that the preparation keeps microbial activity within the wide range of pH 5-9, so that the preparation can adapt to more types of soil including acidic, neutral and alkaline soil, the application range of the preparation is expanded, the preparation is prepared through the freeze-drying technology, and the application range of the preparation is widened. The preparation can effectively protect the activity of microorganisms and reduce damage to cells in the drying process, so that the high survival rate of siamensis amensis in the preparation is ensured, and compared with a traditional preparation, a higher biological control effect is provided.

The invention discloses a microbial agent for preventing and treating typical diseases of tomatoes, and belongs to the technical field of microorganisms. The microbial agent comprises the following components: trichoderma evergreen, trichoderma hamatum, bacillus licheniformis and azotobacter chroococcum in a volume ratio of 1: 1: 1: 1. Fungi and bacteria with disease-resistant and growth-promoting effects are reasonably combined and compounded for use, various typical tomato diseases are effectively improved, through combined and compounded use of the fungi and bacteria of the microbial agent, the practical problems that prevention and control of a single biocontrol strain are unstable and the persistence is weak are solved, the biocontrol effect is greatly improved, and the method is suitable for large-scale popularization and application. Particularly, the composition has a remarkable prevention and treatment effect on typical tomato diseases such as root rot, gray mold, fusarium wilt and early blight; in addition, the microbial agent can be harmonious and compatible with soil ecology, the advantages of strains can be brought into full play, and the tomato yield and quality are effectively guaranteed and improved.

The invention discloses a fertilizer for overcoming continuous cropping obstacles of pinellia ternata, which is prepared by mixing traditional Chinese medicine residues and animal waste and composting, so that on one hand, the anti-pathogen characteristic of clearing heat and drying dampness or clearing heat and removing toxicity and the medicinal components for sterilization are fully exerted; on the other hand, the organic matter content of the fertilizer is enriched, the PH value and structure of soil are improved, the porosity and matrix volume weight of the soil are increased, a microenvironment is provided for inhibiting pathogenic bacteria in the soil through a microbial agent, and the problems of soil degradation, disease accumulation and the like which are prone to occurring in continuous cropping planting of pinellia ternate are solved through cooperation of multiple aspects; by improving the soil environment, enhancing the biological control ability and providing comprehensive and balanced nutrition, continuous cropping obstacles are effectively relieved, and a powerful guarantee is provided for continuous high yield of pinellia ternate.

This invention discloses a method for controlling bacterial wilt and soil antibiotic resistance genes based on a bio-liquid-microorganism-silicon synergy, belonging to the fields of agricultural microbial technology and soil remediation technology. The method includes the following steps: S1, using straw from fields frequently affected by bacterial wilt as raw material, a renewable antibacterial bio-liquid rich in phenolic and ketone compounds is prepared; S2, Bacillus subtilis is subjected to stress-adaptive screening and continuous domestication culture to obtain domesticated strains; S3, the domesticated strains are expanded to prepare a biocontrol agent with enhanced antagonistic ability and environmental tolerance; S4, under pot conditions, the optimal combination of silicon fertilizer, antibacterial bio-liquid, and stress-adaptive biocontrol agent is screened to clarify its role in reducing the spread of antibiotic resistance genes in the soil-plant continuum; S5, under field conditions, silicon fertilizer, antibacterial bio-liquid, and stress-adaptive biocontrol agent are synergistically applied to inhibit soil-borne diseases and simultaneously reduce the spread of antibiotic resistance genes in the soil-plant continuum. This invention has the advantages of stable and sustained control effect and high ecological safety. It can effectively protect the healthy growth of crops and reduce the risk of the spread of resistance genes.

The utility model discloses an oviposition collecting device suitable for large-scale breeding of cantheconidea furcellata, which comprises a nymph breeding net cage, a connecting pipe, an adult breeding net cage, an oviposition suspension line, an oviposition frame, a feed basin and oviposition cloth, one end of the connecting pipe is communicated with the nymph breeding net cage, and the other end of the connecting pipe is communicated with the adult breeding net cage; the oviposition suspension line is arranged at the top of the adult feeding net cage, and the oviposition frame and the feed basin are arranged in the adult feeding net cage; and the spawning cloth is suspended on the spawning suspension line and / or the spawning frame. The device has the advantages that the difficulty of manual pairing and manual egg taking can be greatly reduced, the labor cost in the breeding process is remarkably reduced, meanwhile, the frequency of manual water replenishing is reduced, the breeding efficiency and the egg taking efficiency are remarkably improved, and the device is very suitable for large-scale breeding and egg taking of cantheconidea furcellata. The resource guarantee is provided for further improving the biological control effect and efficiency.

This invention discloses a strain of *Bacillus amyloliquefaciens*, JDF630, and its application in controlling *Fusarium wilt* of *Cotinus coggygria*, belonging to the field of microbial technology. The *Bacillus amyloliquefaciens* strain JDF630 described in this invention was deposited on July 13, 2022, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC NO. 25289. This invention demonstrates that strain JDF630 exhibits broad-spectrum inhibitory activity against various plant pathogens. Treatment with JDF630 significantly reduces the damage caused by *Fusarium wilt* to *Cotinus coggygria*, providing a new microbial strain and control strategy for the prevention and control of plant pathogens, demonstrating excellent biological control effects.

The invention relates to a method for preparing a composite antibiological inoculant by using Shuanghuanglian herb residues. The method comprises the following steps: 1) preparing a seed solution; 2) preparing a Shuanghuanglian medicine residue solid fermentation culture medium; and (3) preparing the biocontrol inoculant: uniformly inoculating the seed solution prepared in the step (1) into a Shuanghuanglian decoction dreg solid fermentation culture medium according to the inoculum size of 8-12%, fermenting at 33-37 DEG C for 9-11 days, and drying at room temperature to obtain the composite biocontrol inoculant. The preparation method is simple, low in cost and convenient to use, the Shuanghuanglian medicine residues with bacteriostatic activity serve as a solid culture medium to conduct solid fermentation on antagonistic strains F-1 and HT-9 to prepare the composite biocontrol inoculant, the obvious biocontrol effect is achieved, the obvious prevention and treatment effect on the peanut southern blight is achieved, and the social and economic benefits are obvious.

This invention discloses a method for enhancing the ability of Trichoderma to control plant diseases, belonging to the field of biocontrol technology. This invention is the first to identify the rice black spore divirus NoPV1-COTG-19 carried by *Trichoderma guiyuan* COTG-19, and confirms that this virus can enhance the inhibitory ability of *Trichoderma* against plant pathogens. Plate confrontation experiments show that the virus-carrying COTG-19 strain has a significantly higher inhibition rate against *Pseudomonas aeruginosa* than the virus-free COTG-19-FV strain. Pot and field trials show that the NoPV1-COTG-19 strain has excellent control effects against *Tetracentron sinense* canker, and the NoPV1-COTG-19 virus can be horizontally transmitted to other *Trichoderma* strains, significantly enhancing the sporulation capacity of infected *Trichoderma* strains. This invention provides a novel technical approach to improving the biocontrol efficacy of *Trichoderma*, and has broad application prospects in the field of green control of plant diseases.

The invention discloses a biological bacteriostatic agent, a preparation method and application in prevention and control of postharvest diseases of fruits and vegetables, the bacteriostatic agent is bacillus coagulans metabolite VOCs, the preservation number of the bacillus coagulans is CGMCC NO.9951, bacteriostatic substances in the metabolite VOCs include but are not limited to benzaldehyde, acetic acid, isobutyric acid and isovaleric acid, and the bacteriostatic substances in the metabolite VOCs include benzaldehyde, acetic acid, isobutyric acid and isovaleric acid. The preparation method of the biological bacteriostatic agent comprises the following steps: diluting activated bacillus coagulans, coating the surface of an LB culture medium with the diluted bacillus coagulans, and culturing at 37 DEG C for 48 hours. The application method of the biological bacteriostatic agent in germ control in fruit and vegetable storage comprises the following steps: putting sweet potatoes and a bacillus coagulans agent in a packaging carton together, and storing in a 28 DEG C environment. The bacillus coagulans CGMCC 9951 is a generally considered safe (GRAS) strain, metabolites are safe and non-toxic, and the bacillus coagulans CGMCC 9951 is environment-friendly, convenient to produce and capable of being applied on a large scale. Chemical agents can be partially replaced for application, pesticide residues are reduced, and the storage quality of sweet potatoes is improved.

The present invention discloses a strain of Fusarium oxysporum and its application, and belongs to the technical field of plant endophytic fungi. The present invention screens and obtains a strain of plant endophytic fungi: Fusarium oxysporum G3‑23, with a deposit number of CGMCC No. 41495. Experiments have found that Fusarium oxysporum G3‑23 has excellent inhibitory effects on multiple pathogens such as Helicoverpa spp., Helicoverpa zeae, Fusarium graminearum, and Fusarium asiatica, indicating that Fusarium oxysporum G3‑23 has broad-spectrum antibacterial activity. The Fusarium oxysporum G3‑23 has significant biological control effects on leaf spot of gramineous grass, leaf spot of corn, and fusarium head blight of wheat. The present invention provides new strain resources for biological control of plant diseases and lays a theoretical foundation for the effective prevention and control of plant diseases.

This invention discloses a biocontrol bacterium for bacterial wilt, YL-Ste-01evo (accession number CCTCC NO: M2022939); a biocontrol bacterium for bacterial wilt, YL-Ste-01 (accession number CCTCC NO: M2022324); and a lytic bacteriophage, YL-Ste-01P (accession number CCTCC NO: M2022460). This invention discloses the application of biocontrol bacterium YL-Ste-01evo, or biocontrol bacterium YL-Ste-01, or biocontrol bacterium YL-Ste-01evo in synergy with bacteriophage YL-Ste-01P in the control of soil-borne bacterial wilt in tomato. The biocontrol bacterium YL-Ste-01 can significantly inhibit the growth of Ralstonia solanacearum and reduce the incidence of bacterial wilt in tomato plants. Through phage stress, YL-Ste-01 evolved into a biocontrol bacterium YL-Ste-01evo with stronger phage resistance and better antibacterial effect.

The invention belongs to the field of pest attractants. The invention discloses a method for trapping and killing winged aphis gossypii glover by using an attractant, which comprises the following steps: preparing DL-limonene, m-xylene, styrene, 2-decanol and dibutyl phthalate into the attractant in proportion, and embedding the attractant into preheated agar gel; when the attractant is applied, the agar gel embedded with the attractant is placed in the natural rubber lure core head and sealed to prepare lure cores, the lure cores are attached to the two sides of the yellow sticky trap, the yellow sticky trap is placed in a cotton field, and the winged aphis gossypii glover is trapped. The components of the attractant are all plant volatile components, so that the attractant is green, safe and environment-friendly, and does not cause environmental pollution; the attractant components are low in cost, and the lure and the trap device are easy to manufacture. By trapping the first-generation parthenogenetic winged aphis gossypii gossypii immigrated into the cotton field in the cotton seedling stage, the cardinal number of the cotton field aphis gossypii gossypii gossypii gossypii gossypii gossypii gossypii gossypii gossypii gossypii gossypii gossypii gossypii gossypii gossypii gossypii

The present application belongs to the field of microbial technology, and particularly relates to a strain of controvirus VP-1 and a fermentation liquor and application thereof. The present application provides a strain of controvirus VP-1, which has been biologically preserved with a preservation number of CCTCC NO: M20221633. The controvirus VP-1 of the present application can be used for preventing and treating plant diseases and promoting plant growth. The results of examples show that the controvirus VP-1 obtained by the present application can promote the growth of rape roots and has a good biocontrol effect on sclerotinia stem rot of rape, thereby providing a new and beneficial biocontrol resource for biological control of crop fungal diseases.

This invention belongs to the field of microbial technology and discloses a strain of *Bacillus zeylanus* and its application in the control of plant diseases, particularly its application in the control of root rot in wolfberry. The *Bacillus zeylanus* strain (… Paenibacillus zeae This strain, isolated from the rhizosphere soil of wolfberry plants suffering from root rot, is designated R2 and has been preserved under the CGMCC No. 36137. It inhibits the growth of various fungal pathogens, including *Lycium chinense*, the causal agent of wolfberry root rot, produces cellulase and amylase, and can induce plant... PR1 , CAT2 The strain expressed defensive genes. Pot experiments showed that it achieved a 66.66% control effect against wolfberry root rot, significantly reducing the incidence and disease index, and providing an effective biological resource for the green control of plant diseases, especially wolfberry root rot.

The invention relates to the technical field of microorganisms, in particular to Burkholderia pyrrocinia ZR-28 and application of the Burkholderia pyrrocinia ZR-28, the Burkholderia pyrrocinia ZR-28 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, the preservation date is May 17, 2024, and the preservation number is CGMCC NO.30588. The invention further discloses a preparation method of the Burkholderia pyrrocinia ZR-28 and application of the Burkholderia pyrrocinia ZR-28. The Burkholderia pyrrocinia ZR-28 provided by the invention not only can promote the growth of plants such as strawberries and corns, but also has a biological control effect on agricultural diseases such as strawberry gray mold and corn southern leaf blight, has an excellent antagonistic effect on pathogenic bacteria of various plant diseases, and has wide application prospects and development space.

The invention discloses fusarium oxysporum and application thereof, and belongs to the technical field of plant endophytic fungi. According to the invention, a plant endophytic fungus, namely fusarium oxysporum G3-23, is obtained through screening, and the preservation number is CGMCC No. 41495. Experiments discover that the fusarium oxysporum G3-23 has an excellent inhibition effect on a plurality of pathogenic bacteria such as wheat root rot bipolaris, maize bipolaris, fusarium graminearum and fusarium asiaticum, which means that the fusarium oxysporum G3-23 has broad-spectrum antibacterial activity, and the fusarium oxysporum G3-23 has an excellent inhibition effect on a plurality of pathogenic bacteria such as wheat root rot bipolaris, maize bipolaris, fusarium graminearum and fusarium asiaticum. The fusarium oxysporum G3-23 has a remarkable biological control effect on the gramineous elymus dahuricus leaf spot disease, the corn leaf spot disease and the wheat scab. The invention provides a new strain resource for biological control of plant diseases, and lays a theoretical foundation for effective control of plant diseases.

The invention discloses a plant extract for preventing and treating walnut black spot disease, a preparation method and application thereof, and belongs to the technical field of plant protection and biological control; the plant extract for preventing and treating walnut black spot disease is an Artemisia macrophylla extract, and its antibacterial components include cypermethrin. The plant extract or cypermethrin is used for preventing and treating walnut black spot disease, which can effectively inhibit the growth of pathogenic bacteria, reduce the incidence of walnut black spot disease, and reduce the size of walnut black spot disease spots.

This invention discloses a strain of *Chlorella vulgaris* TR-6 without phospholipase and its application in the control of rice blast, relating to the field of microbial technology. The preservation number of *Chlorella vulgaris* TR-6 is CCTCC NO: M 2026492. *Chlorella vulgaris* TR-6 significantly inhibits the growth of rice blast fungus and significantly inhibits the formation of appressoria of the pathogen. Its application in the control of rice blast shows good biocontrol efficacy and high safety, with no negative effects on rice germination and growth, and can even promote rice seedling growth. The application of this invention can significantly reduce the use of biochemical agents in the treatment of rice diseases.