181results about How to "Good biological control effect" patented technology

The invention relates to Bacillus velezensis with broad-spectrum disease resistance and application of Bacillus velezensis. The Bacillus velezensis BCP6 is preserved in the China General Microbiological Culture Collection Center in May 15, 2019, and the preservation number is CGMCC No. 17805. The Bacillus velezensis provided by the invention has a remarkable inhibition effect on bacterial soft rot and sclerotinia sclerotiorum of lettuces, and meanwhile has broad-spectrum antibacterial activity on plant pathogenic fungi such as Botrytis cinerea, Fusarium solani, F.oxysporum, Penicillium sp. and Rhizoctonia solani and plant pathogenic bacteria such as Acidovorax citrulli, Pseudomonas syringae, Xanthomonas oryzae, Ralstonia solanacearum and P. marginalis. Meanwhile, the Bacillus velezensis has salt and alkali resistance and has significant biocontrol application prospects.

The invention relates to a trichoderma harzianum composite bacteria culture and application of the same in the aspect of plant protection, and belongs to the technical field of biology. The strain of the trichoderma harzianum composite bacteria culture is preserved in the China General Microbiological Culture Collection Center (CGMCC), and classified and named as Trichoderma harzianum MDCGTH 18, with a preservation number of CGMCC NO.2965. The Trichoderma harzianum MDCGTH 18 composite bacteria culture utilizes antagonistic microbes for biological prevention and treatment, simultaneously has the functions of inducing the disease resistance of plants and increasing the yield, has obvious effect of controlling aspen root rot, apple root rot, citrus yellow shoot, watermelon fusarium wilt, tobacco bacterial wilt, wheat scab, full rot, root rot, banded sclerotial blight, cucumber powdery mildew, downy mildew, vegetable gray mold, fusarium wilt and various other epidemic diseases, can be independently taken as a biological control microbial inoculum for plant diseases, and can also be mixed with other biological antiseptics, plant antibiotics, plant growth regulators and the prior antiseptics to prepare various biological antiseptics for controlling different plant diseases.

The invention discloses a bacillus velezensis strain CSQXDZ26, which is bacillus velezensis (Bacillus velezensis) with the preservation number of CGMCC NO. 17935. The bacillus velezensis CSQXDZ26 hasremarkable antagonistic activity on bacterial plant diseases such as Xanthomonas oryzae pv. Oryzae, Xanthomonas oryzae pv. oryzicola, and xanthomonasaxonopodis pv. glycines AND THE LIKE; meanwhile, the bacillus velezensis CSQXDZ26 also has antibacterial activity on fungal plant diseases such as dithiorella gregaria, Fusarium graminearum, Fusarium oxysporum f.sp.Cucumerinum, Fusarium oxysporum f.sp. momordicae, Alternaria solani or Alternaria alternate, and also has excellent inhibition effect on common food spoilage bacteria such as bacillus subtilis, proteusbacillus vulgaris and staphylococcus aureus.

The invention relates to bacillus subtilis Pc3 and a use of bacillus subtilis Pc3 in preparation of a fermentation supernatant for preventing and controlling plant pathogenic fungi, and relates to bacilli. The bacillus subtilis Pc3 has a preservation number of CCTCC NO: M2013470. The bacillus subtilis Pc3 can prevent and control plant pathogenic fungi such as gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani and fusaria. A rape sclerotinia sclerotiorum prevention and control pot experiment proves that the bacillus subtilis Pc3 has good biological-prevention and control effects and has prevention and control efficiency of 95.6%. The fermentation supernatant of the bacillus subtilis Pc3 can be prepared by simple processes and has a wide fungus inhibition spectrum and good biological prevention effects.

The invention provides a bio-control bacterium foliage fertilizer, and preparation and application thereof. The bio-control bacterium foliage fertilizer is prepared from the following materials in parts by weight: 1-3 parts of core bio-control bacteria, 35-85 parts of biochemical fulvic acid potassium, 10-50 parts of active SiO2, 2-6 parts of an enzyme activity substance, and 2-6 parts of a plant surfactant. The core bio-control bacteria contain bio-control promoting bacteria and protein decomposing bacteria; the bio-control promoting bacteria comprise bacillus subtilis and trichoderma harzianum. The core bacteria in the bio-control bacteria foliage fertilizer can rapidly grow on the surface of a plant in a field planting manner and form a 'bio-control membrane' so as to prevent infection of pathogenic bacteria at the surface of the plant; the substances, such as antibiotics and phytohormone, are generated in the processes of interaction with the pathogenic bacteria and hyperparasitism, and a plurality of diseases of crops can be prevented, resisted or directly killed; the bio-control bacteria foliage fertilizer enables the crops to have solid plant cell wall tissue, so that the ability of plant epidermis for directly resisting incursion of the pathogenic bacteria and insect pests is enhanced, and healthy growth of the crops is facilitated; the target of using a little of pesticide or not is achieved, and the environmental pollution is alleviated. Thus, the yield and the quality of the crops are improved.

The invention discloses bacillus methylotrophicus F7 and an application thereof. The invention aims at providing a strain which has a plurality of beneficial properties, namely producing siderophore, cellulase and protease, producing auxin, producing ammonia and the like; the strain not only can be used for effectively inhibiting various pathogenic bacteria such as ralstonia solanacearum, fusarium oxysporum f.sp.cubense, colletotrichum gloeosporioides, fusarium oxysporum f.sp cucumerinum but also can promote plant growth. In accordance with the technical scheme, the bacillus methylotrophicus F7 is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 1, 2016, with preservation number of CGMCC No.12124; in addition, five surfactin homologs, six fengycin homologs and three iturin homologs, which have an antibacterial effect, are extracted from fermentation liquid of the bacillus methylotrophicus F7; and the bacillus methylotrophicus F7 belongs to the field of agricultural biotechnology.

The invention provides bacillus amyloliquefaciens cd5bc. The strain is classified and named as Bacillus amyloliquefaciens cd5bc and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO:M 2016232, and the preservation time is April 28, 2016. The invention further provides application of the strain in the aspect of preventing and controlling crop diseases and a biocontrol preparation prepared from the strain. The strain has a very significant preventing and controlling effect on red pepper phytophthora blight, the biocontrol effect reaches up to 100% after inoculation is conducted for 15 days, and the biocontrol effect reaches up to 82.36% after inoculation is conducted for 60 days. The bacillus amyloliquefaciens cd5bc has excellent disease-resistant effects on multiple crop diseases, the bacteriostasis rates of the strain on pathogenic bacteria of red pepper sclerotinia blight, bitter gourd wilt, cucumber wilt, cucumber scabs, rice blast, a rice sheath blight disease, broad bean chocolate spots and broad bean brown spots all reach up to 70% or above, and the bacteriostasis rate of the strain on the broad bean chocolate spots and broad bean brown spots reaches up to 100%.

The invention discloses a compound biological preservative. The compound biological preservative comprises the components as follows: 0.1-5 g of component B is added in each 1L of component A, the component A is fermentation liquor of Bacillusamyloliquefacienssubsp.plantarum BGP20 strains, and the component B is natamycin. The compound biological preservative is used for preventing and controlling diseases of fruits and vegetables in a storage period and particularly can be used for simultaneously preventing and treating bacterial soft rot and various fungal diseases (for example, phytophthora capsici, rhizopus soft rot, pythium blight and the like) of fruits and vegetables in the storage period, avoiding or reducing residual chemical pesticides and agricultural antibiotics and maintaining the quality of the fruits and vegetables in the storage period, so that the compound biological preservative has good economic and social benefits.

The invention discloses streptomyces albidoflavus Actin-1. The streptomyces albidoflavus Actin-1 can grow and propagate with apple tree rot pathogenic bacterium mycelium as nutrition, long-term colonization of biocontrol bacteria on apple tree rot scabs is promoted, a long-term biological prevention and treatment function is achieved, meanwhile, the streptomyces albidoflavus Actin-1 is induced to generate multiple ectoenzyme cell wall hydrolytic enzymes, and pathogenic bacterium cells disintegrate in combination with the enzyme dissolving function; high antibacterial active matter can be generated, the bacteriostasis rate on the apple rot pathogenic bacteria is 89.82%, a good antibacterial effect is achieved on botryosphaeria berengeriana and other pathogenic bacteria, the bacteriostasis rate ranges from 76.08% to 87.10%, and broad spectrum bacteriostasis performance is achieved. The streptomyces albidoflavus Actin-1 is adopted as main biocontrol bacteria for preventing and treating the apple tree rot and other fruit and vegetable pathogenic bacteria, and the advantages of being good in prevention and treatment effect (100%), high in efficiency, low in recurrence rate (0), high in environment adaption capacity, high in stability, not likely to generate resistance to drugs and the like are achieved. Important significance is achieved on improving the prevention and treatment effect on the apple tree rot and other fruit and vegetable pathogenic bacteria, preventing pathogenic bacterium relapse and protecting the environment.

The invention provides acid-resistant pseudomonas protegens CLP-6 and application thereof. According to the acid-resistant pseudomonas protegens CLP-6 and the application thereof, the bacterial strainof the pseudomonas protegens CLP-6 is preserved in China General Microbiological Culture Collection Center on October 27th, 2016, and the biological preservation number of the pseudomonas protegens CLP-6 is CGMCC No.13205; the bacterial strain of the pseudomonas protegens CLP-6 has acid resistance, is good in disease prevention and plant growth promotion effects and high in stress resistance, hasthe antagonistic activity of pathogenic bacteria, is wide in soil acidity tolerance range and wide in antibacterial spectrum, and has application and development potential in prevention and treatmentof bacterial wilt, black shanks and brown spots; and meanwhile, under the general background that the acidification conditions in China is increasingly severe, the research on the bacterial strain ofthe pseudomonas protegens CLP-6 has reference significance in prevention and treatment of plant diseases under various soil qualities, a microbial resource is provided for green prevention and control over the bacterial wilt, the black shanks and the brown spots in acid soil, and secondary metabolites of microorganisms are excavated, so that an important foundation can be laid for the biocontrolefficacy on the bacterial wilt, the black shanks and the brown spots in the acid soil.

The invention provides a method for breeding aphidiidae through a flue-cured tobacco seedling cultivation greenhouse. The method includes the steps of regarding a flue-cured tobacco seedling cultivation greenhouse which is conventionally used as a foundation, setting up a stud aphid and stud bee cultivation base matched with the flue-cured tobacco seedling cultivation greenhouse, cultivating bee breeding tobacco seedlings through cultivating and reserving tobacco seedlings in a bee breeding chamber of the flue-cured tobacco seedling cultivation greenhouse, and conducting aphid inoculation and bee inoculation on the tobacco seedlings through stud aphids and stud bees till bee collecting and bee releasing are conducted. The operation is easy to conduct, the bee breeding effect is good, the cost is low, the method plays an overall role in preventing and controlling a whole tobacco planting area and myzus persicae of other corps in the whole tobacco planting area, therefore, a good biological control effect is achieved, and the method is suitable for popularization.

The invention discloses a pseudomonas nitroreducens and application thereof in degradation of DSF (diffusible signal factor) and / or DSF analogues. The pseudomonas nitroreducens disclosed by the invention is named as HS-18, and is preserved in China Center for Type Culture Collection on May 12, 2017 with a preservation number of CCTCC NO:M2017257. The strain has stable activity, can take DSF as thesole carbon source for growth, can completely degrade DSF within 24h, has a significant and rapid degradation effect on DSF, and has high application value in quorum quenching direction, black rot disease inhibition, and control of the harm of DSF mediation dependent pathogenic bacteria. The pseudomonas nitroreducens provided by the invention can reduce the pesticide abuse problem, and at the same time provides a new idea for biological control of the harm of phytopathogen.

The invention discloses paecilomyces lilacinus wettable powder, a preparation method and application thereof. The paecilomyces lilacinus wettable powder comprises the following components of paecilomyces lilacinus spore powder or fermentation liquor, a vector, a wetting agent and a dispersing agent, wherein the wetting agent is selected from any one or more of D1002, NNO or sodium lignin sulfonate; and the paecilomyces lilacinus wettable powder also comprises an ultraviolet protection agent or / and a synergist. According to the paecilomyces lilacinus wettable powder, the traditional wetting agents and dispersing agents are screened and optimized to obtain a specific species and a dosage having a smaller influence on the growth of the paecilomyces lilacinus; in addition, the ultraviolet protection agent for preventing paecilomyces lilacinus spores from being damaged by ultraviolet and the synergist for promoting plants to take roots or increasing the meloidogyne killing effect are further screened and obtained. The invention further provides the preparation method of the paecilomyces lilacinus wettable powder and the application of the wettable powder in preventing and controlling plant meloidogyne.

The invention belongs to the technical field of biocontrol on plant diseases and particularly relates to separation and identification of a rice sheath blight biocontrol strain RS-BC, and a microbial agent containing the rice sheath blight biocontrol strain RS-BC, a preparation method of the microbial agent and application of the microbial agent to control of rice sheath blight. A separated strain is Burkholderia (Burkholderia vietnamiensis) RS-BC, which is collected in the China Center for Type Culture Collection with the collection number of CCTCC NO: M2011049. The invention further relates to the microbial agent prepared from the rice sheath blight biocontrol strain RS-BC, the preparation method of the microbial agent and the application of the microbial agent to the control of the rice sheath blight.

The invention discloses a novel chitinase gene of nematode bio-control fungi lilac paecilomyces with a gene sequence table as seen in SEQ ID NO: 1, and meanwhile discloses a method for cloning the chitinase gene of the lilac paecilomyces. The method comprises the steps of using lilac paecilomyces RNA with highest enzyme activity as a template, designing and expanding a pair of degenerate primers to get the chitinase gene fragments of the lilac paecilomyces, obtaining gene 5' terminal and 3' terminal sequences from a lilac paecilomyces cDNA chain through SMART RACE RT-PCR technology, and connecting the three sequences to obtain a novel cDNA full-length sequence of the chitinase gene (PL Chil). The invention provides important technological base for transforming the chitinase gene of the lilac paecilomyces to other nematode bio-control fungi by using bioengineering technology, realizing the heterologous expression of the gene, and improving the expression quantity of the chitinase gene of the nematode bio-control fungi and the bio-control ability of plant nematodes.

The invention provides a streptomyces sp. strain YT027 which is preserved in China General Microbiological Culture Collection Center with the address of 3#, No.1 Yard, Beichen West Road, Chaoyang District, Beijing (100101) on January 23rd, 2013, and the preservation number is CGMCC No. 7196. The streptomyces YT027 provided by the invention has higher antagonistic activity to Phytophthora colocasiae Racib., the fermentation culture liquid of the streptomyces YT027 has good biocontrol effects on sweet potato diseases in the preservation period, can avoid or reduce chemical pesticide residue, remarkably prolong the storage period of sweet potatoes and maintain the quality of the sweet potatoes, and has better economic and social benefits.

The invention relates to bacillus halotolerans SY1836 and application thereof. The bacillus halotolerans SY1836 has an accession number of CGMCC No. 20080 and is a biocontrol bacterium separated and obtained from saline-alkaline soil. According to the application, verified by experiments, the bacillus halotolerans SY1836 has a relatively good effect of antagonizing various botanical pathogenic bacteria, can be used for effectively lowering a disease index of capsicum when the bacillus halotolerans SY1836 is applied to a growth process of the capsicum, and has a remarkable phytophthora blight control efficiency. The bacillus halotolerans SY1836 simultaneously can be used for remarkably improving plant height and fresh weight of the capsicum during growth and has an important significance inphytophthora blight prevention and control and planting of the capsicum.

The invention relates to a method for preparing a soil composite biological repairing agent by using maize straws and the application of the repairing agent in soil property improvement. According to the method, maize straws which are low in cost and renewable are adopted as raw materials, treated trichodermahamatums T22 and T8-2 are taken as strains, and then liquid-liquid two-phase mixed strainfermentation technology is carried out to prepare the soil composite biological repairing agent. The repairing agent has a good repairing effect on soil, a good degradation effect on pesticide residues in soil, a strong restraining or killing effect on soil spreading pathogenic bacteria and a promoting effect on crop growth and is harmless to ecological environment.

The invention discloses Trichoderma viride engineering bacteria, which comprises endochitinase genes, was named as green Trichoderma viride R65 and was preserved in 'CCTCC' on December 29, 2008. The preserving number is CCTCC NO: M 208265. The invention also discloses the applications of the strains in controlling vegetables. The highest vigor of the chitinase of the strains can achieve 77.4U / ml, which is 3.67 times of the strains of an original host. The strains have stronger antagonism on a plurality of plant pathogenic fungi like cucumber gray mold, Aspergillus niger, rhizoctonia solani kuhn, wheat red mould, cotton Phytophthora, and the like, and is an engineering bacteria which have more application values in controlling vegetable.

The invention discloses an antibiological inoculant for controlling apple tree canker, and application of the antibiological inoculant. The antibiological inoculant can grow and propagate directly by using pathogenic mycelium as nutrition, and can induce the self to produce multiple extracellular enzyme capable of degrading cell walls of pathogenic bacteria; a streptomyces albidoflavus Actin-1 strain, colonized at disease spots of the apple tree canker for a long time, is activated and subjected to spore production, so that spore suspension is prepared; after that, a fermented seed solution is prepared by continuously carrying out liquid fermentation; finally, the fermented seed solution is inoculated into a solid fermentation medium, and is then fermented, dried in the air, crushed and screened so as to be prepared into spore raw powder; the spore raw powder is then scientifically screened and compounded by using auxiliary agents such as an ultraviolet protective agent, a dispersing agent and a carrier. The antibiological inoculant provided by the invention has the multiple advantages of being good in control effect, high in efficiency, low in recurrence rate and high in environmental adaptability and stability, not easy in producing drug-resistance, and the like, thus being a first choice for biological control; the antibiological inoculant has a great significance for improving the preventing and treating effect of fruit and vegetable pathogenic bacteria such as the apple tree canker, preventing pathogen recurrence and protecting environment.

The invention provides bacillus subtilis LX13 with the collection number of CGMCC No. 8805. The bacillus subtilis LX13 can be used for preventing and treating crown rot, southern blight and root rot of peanuts. Compared with the prior art, the bacillus subtilis LX13 has the advantages that the bacillus subtilis LX13 has obvious antagonism on pathogenic bacteria of the peanuts, and can serve as a potential biocontrol strain; the biocontrol effect is good, and the bacillus subtilis LX13 has obvious prevention and treatment effect on the crown rot, the southern blight and the root rot of the peanuts; growth and yield of the peanuts can be promoted remarkably; the bacillus subtilis LX13 is convenient to use, has quick action and long action time, and is easy to prepare and store and convenientto apply and popularize.

The invention discloses broad-spectrum fungus-resistant bacillus and application thereof to preventing wheat scab and relates to bacillus. The preservation number of the bacillus (Bacillus sp.)DY26-010 is CCTCC NO:M2015407. Supernatant liquid of the bacillus is antagonistic to fusarium spp, gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia, mutualism alternaria, botrytis cinerea and colletotrichum dematium. Fermentative supernatant liquid of the bacillus DY26-010 has high antagonism on pathogenic bacteria and fusarium spp of wheat scab, the preventing effect can be 57.1% in a potted experiment of wheat scab, and the bacillus can be used for preparing the fermentative supernatant liquid applied to preventing wheat scab. A biological prevention method of wheat scab is provided, no chemical pesticide is used, the bacillus has the advantages of being non-toxic, not prone to forming a pesticide-resistant environment, biologically safe and the like, and application prospects are good.

The invention discloses bacillus velezensis HY19 and application thereof. The strain Bacillus velezensis HY19 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is January 4, 2021, and the preservation number is CGMCC No.21590. The strain can generate various water-soluble and volatile antibacterial substances, can effectively antagonize various plant pathogenic fungi, is wide in antibacterial spectrum and strong in antibacterial effect, has a relatively good biological prevention and treatment effect on flue-cured tobacco brown spot, is harmless to the ecological environment, and is not easy to cause drug resistance of pathogens. The strain has the characteristics of high breeding speed, high environmental adaptability, simple nutritional requirements, wide disease-resistant spectrum and the like, and is a novel biocontrol microorganism which is safe, efficient and capable of preventing and treating fungal diseases of plants in a broad-spectrum manner. The biocontrol microbial agent of the invention is simple in preparation method, easy for industrial production, and has good development and application prospects.

The invention discloses a novel beta-1,6-glucanase, an encoding gene thereof, and applications of the encoding gene. The invention provides the beta-1,6-glucanase gene belonging to the glucoside hydrolase system, wherein the nucleotide sequence of the beta-1,6-glucanase gene is shown in SEQ ID NO.1, and the protein (amino acid) sequence of the encoded outer membrane glucoside hydrolase is shown in SEQ ID NO.2. The beta-1,6-glucanase can effectively prevent the infection of plant pathogenic fungi to plants. An engineered strain established by the gene realizes the prokaryotic expression of the beta-1,6-glucanase gene, and verifies the function of the glucoside hydrolase of the beta-1,6-glucanase gene, the result shows that GluM can effectively inhibit the germination of rice blast spores, and decompose the rice blast spores. After the beta-1,6-glucanase gene is transplanted into the dicotyledonous model plant arabidopsis thaliana and the monocotyledonous model plant rice, the result shows that the obtained transgenic plants have good anti-infection effects for grey mould and rice blast.

The invention relates to Antarctic-derived Bacillus sp.N311 and an application of Bacillus sp.N311 for preventing and treating phytopathogenic fungi, which relate to Bacillus sp. A preservation number of the Bacillus sp.N311 is CCTCC NO: M 2015607. The Bacillus sp.N311 can be used for preparing a fermentation supernatant for preventing and treating phytopathogenic fungi. The phytopathogenic fungi comprise fusarium, gloeosporium musarum, Paecilomyces variotii, sclerotinia sclerotiorum, Alternaria longipes, Trichoderma viride, Rhizoctonia solani, alternaria alternate, botrytis cinerea and Colletotrichum gloeosporioides. The Bacillus sp.N311 has good biological prevention and treatment effects in Rape Sclerotinia Rot pot culture prevention and treatment tests, and prevention and treatment effects can reach more than 50%. The Bacillus sp.N311 fermentation supernatant has wide fungus-inhibiting spectrum and good biocontrol effect, has advantages of no toxicity, difficult generation of drug resistance, environment safety and biosafety, and has wide application prospect.

The invention provides a strain of bacillus subtilis with disease resistant activity. The bacillus subtilis with disease resistant activity is bacillus subtilis LVLE14, has the preservation date of June 20, 2016, and has the preservation number of CGMCC No.12639. The invention also provides a method for producing a liquid fertilizer by using the bacillus subtilis. The bacillus subtilis is used forfermentation production. The fermentation liquid is added into the liquid organic raw materials according to the mass ratio of 0.1 to 3 percent to obtain the liquid biological fertilizer; meanwhile,the production method of the biological organic fertilizer is provided. The invention also provides application of the bacillus subtilis to the effects of inhibiting the crop diseases and particularlyinhibiting powdery mildew of cucumber and gray mold of cucumber. The bacillus subtilis and the prepared fermentation liquid and fertilizer have the effective biological control effect; the preventionand treatment effect on the powdery mildew of cucumber and the gray mold of cucumber is better.

The invention discloses an aspergillus flavus biocontrol strain realizing resistance on bactericides without producing aflatoxin and application of the aspergillus flavus biocontrol strain. The aspergillus flavus biocontrol strain is named as aspergillus flavus AF054; the preservation number is CGMCC NO.9860; and the preservation date is November 20, 2014. The aspergillus flavus obtained through separation does not generate the aflatoxin; the formation of the aflatoxin in agricultural products can be effectively inhibited; the aflatoxin pollution in the agricultural products is reduced; the agricultural product quality is improved; the resistance on common-use bactericides such as carbendazol, tebuconazole and amistar is realized; the problem of sensitivity of the existing biocontrol strain on the bactericides in a farmland system is solved; and the colonizing capacity and the adaptability of the biocontrol strain in the field are effectively improved, so that the biocontrol effect of biocontrol bacteria is improved.

The invention relates to a biocontrol strain KMXU22 capable of preventing and treating soft rot of konjak and an antibiological inoculant of the biocontrol strain KMXU22, and belongs to the technical field of biopesticide. The preservation number of the strain KMXU22 provided by the invention is CGMCC NO. 6597. The antibiological inoculant is obtained by the following steps of: transplanting the strain KMXU22 into a test tube, carrying out one-cycle activation, and culturing for 48 hours at the temperature of 32 DEG C, wherein LB liquid culture medium in the test tube contains 10g / L tryptone, 5g / L yeast extract and 5g / L sodium chloride, and the pH value is 7.0; transferring an activated bacterial lawn into 600ml of LB culture solution which is filled in a 1000ml conical flask and has the same formula as a., and culturing for 72 hours under the conditions that the rotating speed is 120r / min and the temperature is 32 DEG C, so that zymogeneous bacteria solution is obtained; inoculating the zymogeneous bacteria in a seed tank in the ratio of 1: 200 by inoculation amount, and fermenting for 18-20 hours at the temperature of 32 DEG C, so that fermentation seed strains are obtained; and inoculating the fermentation seed strains into a fermentation tank in the ratio of 1: 20 by the inoculation amount, fermenting for 72-96 hours at the temperature of 32 DEG C, and storing the fermented seed strains as the antibiological inoculant when strain content is more than 2*10<10>-2*10<11> CFU / ml and the pH value is 7.0-7.6. The antibiological inoculant provided by the invention has the advantages that cost is low, no position or pollution is produced, and biocontrol effect is good.

The invention discloses a crystal pear biological antistaling agent based on mixed application of cryptococcus laurentii and antagonistic bacillus, a preparation method and application thereof. 12 strains of antagonistic bacilli are respectively employed for mixed application with the cryptococcus laurentii, they all can improve the postharvest biocontrol effect of crystal pears, and four strains can reach the best effect. Finally, the application concentration and ratio of the antagonistic bacillus and cryptococcus laurentii for a best mixed application effect are determined. Every 1ml of a control agent contains 106-109 antagonistic bacilli and 106-109 cryptococcus laurentii, and the best control effect can be ensured when the bacterial number ratio is 1:1. The cryptococcus laurentii and antagonistic bacillus involved in the biological antistaling agent both have the characteristics of safety and high efficiency, and have the advantages of environmental friendliness, and outstanding biocontrol effects, etc. the mixed bacteria antistaling agent provided by the invention can further reduce the application of chemical bactericides, and has important significance to food safety, environmental protection, agricultural income increase and synergy, and sustainable social development, etc.