Biological control bacillus subtilis, production method, liquid fertilizer and application

A Bacillus subtilis and liquid technology, applied in the field of antagonistic bacteria, can solve problems such as pesticide residues in agricultural products, threats to human health, and environmental pesticide pollution, and achieve the effects of reducing the amount of chemical fertilizers, reducing pesticide residues and pollution, and reducing the amount of pesticides used

Active Publication Date: 2018-03-09
SHANGHAI LVLE BIO TECH
4 Cites 6 Cited by

AI-Extracted Technical Summary

Problems solved by technology

However, a large number of chemical pesticides are used, one is to cause pesticide pollution to the environment, and the...
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Method used

The present invention is a kind of microbial fertilizer with biological control function and production method thereof, utilizes the raw material of the raw material crop fertiliz...
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Abstract

The invention provides a strain of bacillus subtilis with disease resistant activity. The bacillus subtilis with disease resistant activity is bacillus subtilis LVLE14, has the preservation date of June 20, 2016, and has the preservation number of CGMCC No.12639. The invention also provides a method for producing a liquid fertilizer by using the bacillus subtilis. The bacillus subtilis is used forfermentation production. The fermentation liquid is added into the liquid organic raw materials according to the mass ratio of 0.1 to 3 percent to obtain the liquid biological fertilizer; meanwhile,the production method of the biological organic fertilizer is provided. The invention also provides application of the bacillus subtilis to the effects of inhibiting the crop diseases and particularlyinhibiting powdery mildew of cucumber and gray mold of cucumber. The bacillus subtilis and the prepared fermentation liquid and fertilizer have the effective biological control effect; the preventionand treatment effect on the powdery mildew of cucumber and the gray mold of cucumber is better.

Application Domain

BacteriaAlkali orthophosphate fertiliser +8

Technology Topic

ChemistryTreatment effect +10

Image

  • Biological control bacillus subtilis, production method, liquid fertilizer and application
  • Biological control bacillus subtilis, production method, liquid fertilizer and application
  • Biological control bacillus subtilis, production method, liquid fertilizer and application

Examples

  • Experimental program(3)
  • Effect test(1)

Example Embodiment

[0050] Example 1-1
[0051] 1. Microbial liquid: The formula of strain fermentation medium is soluble starch 30g, soybean powder 15g, sucrose 5g, peptone 5g, soybean meal 3g, K 2 HPO 4 3g, MgSO 4 1g, soak enemy 0.5g, make up to 1000ml. The number of effective viable bacteria in the microbial liquid is 9.8×10 9 Pcs/ml.
[0052] The fermentation conditions for the liquid fermentation of Bacillus subtilis LVLE14 are as follows: pH 6.5-7.5, culture temperature 20-40°C, initial rotation speed 100-200 rpm, aeration rate 0.5-1 vvm, culture 35-40 hours, fermentation The spores are formed in the later stage, the spore rate is as high as 95%, and the effective number of viable bacteria in the fermentation broth is more than 10 billion/ml.
[0053] 2. Raw material formula: add according to the following formula. The trace elements are borax and chelated zinc, the ratio is 2:3; the protective agent is sodium benzoate and molasses, the ratio is 1:1.
[0054] Table 4 Ingredients for adding liquid compound microbial fertilizer
[0055]
[0056] 3. Mix the inorganic nutrients with 40% (total mass) water; add the protective agent to the concentrated solution, stir evenly, then add the bacteria solution; finally mix the fully dissolved inorganic nutrients and the concentrated solution containing bacteria, and add the remaining water , Made into liquid compound microbial fertilizer. The technical indicators of the finished fertilizer are as follows.
[0057] Table 5 Main technical indicators of liquid compound microbial fertilizer
[0058]
[0059] 4. The liquid compound microbial fertilizer is packed, sealed and put into storage through the liquid filling equipment.

Example Embodiment

[0060] Example 1-2
[0061] 1. Preparation of solid microbial inoculants: Fermented according to the method of Example 1, and the bacteria liquid after fermentation of biocontrol Bacillus subtilis LVLE14 is adsorbed with peat at the ratio of bacteria liquid: peat = 1:4, and the mixed bacteria are combined The effective number of live bacteria of the agent reaches 1.8×10 9 Pieces/g or more, keep the moisture within 30%.
[0062] 2. Sampling. The hydrolyzed residue produced during the production of amino acids was taken from Henan Lianhua Monosodium Glutamate Co., Ltd.;
[0063] 3. pH adjustment: crush the large hydrolyzed slag into powder or small particles, mix it with powdered lime according to hydrolyzed slag: lime=4:1, smash it with a chain mill, and stack it for 8-12 hours to allow it to fully react;
[0064] 4. Overturning: Use a forklift or manual overturning to let the temperature of the reacted material cool to normal temperature;
[0065] 5. Crushing: The hydrolyzed slag cooled to room temperature is measured. The basic properties are shown in Table 6. The content of heavy metals is far lower than the industry standard of bio-organic fertilizer, so no measurement has been made;
[0066] Table 6: Basic properties of hydrolyzed residue after treatment
[0067]
[0068] 6. Ingredients. The powdery hydrolysis residue is slowly added into the mixer, and the prepared composite microbial inoculant is added in a proportion of 6% by weight.
[0069] 7. Mix. The blender stirs for 3-5 minutes to make the materials fully mixed evenly.
[0070] 8. Smash. The fineness of the material can reach 60-80 mesh through the crusher.
[0071] 9. Packing, measurement and storage.

Example Embodiment

[0072] Example 1-3
[0073] 1. Preparation of solid microbial inoculum: Fermented according to the method of Example 1, and the biocontrol Bacillus subtilis LVLE14 fermented bacteria liquid is adsorbed with peat at the ratio of bacteria liquid: peat = 1:5, and the mixed bacteria are combined The effective number of live bacteria of the agent reaches 1.2×10 9 Pieces/g or more, keep the moisture within 30%.
[0074] 2. Sampling. The sludge produced by adsorption during the salad oil production process was taken from Shanghai Lichang Environment Co., Ltd.;
[0075] 3. pH adjustment: crush large pieces of oil residue into powder or small particles, mix with powdered lime according to oil residue: lime=4:1, smash with a chain grinder and stack for 8-12 hours, let it fully react;
[0076] 4. Overturning: Use a forklift or manual overturning to let the temperature of the reacted material cool to normal temperature;
[0077] 5. Crushing: The oil residue cooled to room temperature is measured. The basic properties are shown in Table 3. The content of heavy metals is far lower than the industry standard of bio-organic fertilizer, so no measurement was made;
[0078] 6. Ingredients. Add the powdered oil residue slowly into the mixer, and add the prepared composite microbial inoculant at a weight ratio of 6%.
[0079] 7. Mix. The blender stirs for 3-5 minutes to make the materials fully mixed evenly.
[0080] 8. Smash. The fineness of the material can reach 60-80 mesh through the crusher.
[0081] 9. Packing, measurement and storage.
[0082] Table 7: Quality test results of bio-organic fertilizer products produced from different raw materials
[0083]
[0084] As can be seen from the above table, from the perspective of antibacterial rate, among solid raw materials, hydrolyzed residues are more suitable for Bacillus subtilis as raw materials.

PUM

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Description & Claims & Application Information

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