The invention discloses an efficient
acclimatization and
transplantation method for sweet potato
virus-free test-tube plantlets, which comprises the following steps: removing fibrous roots from sweet potato
virus-free test-tube plantlets which are subcultured for 20-30 days through
virus detection, treating the sweet potato virus-free test-tube plantlets for 24 hours through an
acclimatization nutrient solution, directly planting the sweet potato virus-free test-tube plantlets into a
transplantation matrix, keeping the temperature of a culture area at 28 + / -2 DEG C, keeping the
relative humidity at 75-80%, and culturing for 3 days under weak light, and supplementing
carbon dioxide to the culture area until the concentration is 1000-1200ppm, culturing for 20-30 days under normal illumination, transferring the sweet potato virus-free test-tube
plantlet with the survival zone
transplanting matrix into a hole tray, filling a
seedling culture matrix to ensure that each hole of the matrix is full, watering, carrying out shading treatment for 3-5 days, and transferring to a normal illumination condition for culturing, so as to obtain the sweet potato virus-free test-tube
plantlet. According to the method, the
seedling age of virus-free
seedling transplanting is reduced to 20-30 days, the cost is saved, the
transplanting survival rate is remarkably increased, the key technical obstacle from test-tube plantlets to propagation seedlings is broken through, and the sweet potato virus-free test-tube plantlets can be quickly applied to production.