141 results about "Phosphorus fertilizer" patented technology

The invention relates to the field of agricultural fertilizer and soil improvement, in particular to a biochar-based slow release fertilizer for improving acidified soil and a preparation method thereof.The biochar-based slow release fertilizer is prepared from, by mass, 60 parts of a biochar system, 5-12 parts of nitrogen fertilizer, 10-18 parts of phosphate fertilizer, 5-12 parts of potash fertilizer, 5-10 parts of an acid soil modifier and 2-5 parts of a slow release film; graded release of nutrients is achieved through a multi-aperture biochar system, the release rhythm is dynamically adjusted according to soil acidity in combination with a three-layer pH response type slow-release film, the problems that existing fertilizer nutrient release is not matched with crop requirements, and the persistence of acidified soil improvement is poor are solved, the pH and buffer capacity of acidified soil can be improved, the nutrient utilization rate is increased, and the soil quality is improved. And most of the raw materials are agricultural and forestry wastes and industrial byproducts, so that the soil conditioner meets agricultural requirements, is suitable for acidified soil, and has a wide application prospect.

The invention discloses application of a phosphorus-solubilizing fungus solid bacterial fertilizer in promoting crop growth, and belongs to the field of environment and the technical field of agriculture. The solid bacterial fertilizer comprises penicillium deserticola and / or penicillium fistulosum, the penicillium deserticola and / or penicillium fistulosum are compounded according to the proportion of 1: 1, agricultural waste mushroom bran serves as a main carrier, and the inoculum size of a bacterial solution is 9 wt%. The bacterial fertilizer can effectively activate insoluble phosphorus in soil, significantly improve absorption and utilization of phosphorus by crops, promote plant growth, increase root length and biomass, increase leaf chlorophyll content, enhance photosynthesis capability, reduce dependence on chemical phosphate fertilizer and realize agricultural waste recycling, and has the advantages of high efficiency, environmental protection and low cost.

The invention provides an organic-inorganic-microbial composite water and fertilizer retention soil conditioner and a preparation method thereof. The organic-inorganic-microbial composite water and fertilizer retention soil conditioner comprises an organic material, a microbial agent and composite phosphate fertilizer slow-release carboxylated modified hydrogel microbeads (inorganic material), the compound phosphate fertilizer slow-release carboxylated modified hydrogel microbeads are mixed with organic materials such as humic acid and a microbial agent, so that the phosphorus slow-release effect and the soil water absorption and retention effect are further improved, and the compound phosphate fertilizer slow-release hydrogel microbeads have high water absorption and retention and fertilizer slow-release functions, can effectively improve soil, increase the soil water retention and phosphate fertilizer effectiveness, improve the enzyme activity of the soil and improve the fertilizer utilization rate. Soil moisture and fertility loss is reduced; the raw materials are wide in source, low in cost, safe and harmless, the preparation process is simple, and the prepared soil conditioner is harmless to the soil environment, has high water absorption and water retention and a fertilizer slow release function, can effectively improve soil and can be used for enhancing the water retention and physicochemical properties of the soil.

The invention discloses a high-calcium suspension fertilizer as well as a preparation method and application thereof, and relates to the technical field of chemical fertilizer production, the high-calcium suspension fertilizer comprises the following components in parts by weight: 20-30 parts of a macroelement fertilizer, 10-15 parts of a medium element fertilizer, 3-5 parts of a microelement fertilizer, 0.5-3 parts of an auxiliary agent and 30-50 parts of deionized water; the macroelement fertilizer is prepared from 30-50 parts of nitrogen fertilizer, 20-30 parts of phosphate fertilizer and 15-20 parts of potash fertilizer; the medium element fertilizer is modified nano calcium carbonate and magnesium carbonate; the trace element fertilizer is prepared from zinc amino acid, sodium tetraborate, ferric sodium ethylenediaminetetraacetate, molybdenum sodium ethylenediaminetetraacetate, manganese sulfate monohydrate and copper sulfate pentahydrate. Therefore, the nano calcium carbonate can be quickly absorbed by the plants, so that calcium ions can be supplemented in time in the growth process of the plants, and the growth of the plants is accelerated.

The invention relates to the technical field of soil ecological restoration and farmland cultivation management, in particular to an ecological regulation and control method for synergistically reducing compound obstacles of yellow soil rice-oil rotation cropland. The method is implemented by stages according to agricultural time: S1, mixing crushed straw, a composite functional microbial agent, a decomposed organic fertilizer and a calcium magnesium phosphate fertilizer into 0-15cm before rice transplanting; s2, after the rice is harvested, deep scarification is conducted by 20-25 cm, and a ridge culture structure is constructed; s3, interplanting astragalus sinicus after the ridges are built and before the oilseed rape is sown; s4, after the oilseed rape is harvested, the oilseed rape straw and the astragalus smicus are turned and pressed by 20-25 cm, lime is applied, and high water holding capacity is maintained in a short time; and S5, interannual circulation. Through microorganism-structure-nutrient coupling, thin, plate and sticky yellow soil is relieved synergistically, aggregate and air permeability are improved, root growth is promoted, and stable yield is increased.

A system of integrated water and fertilizer planting and industrial collaboration for a terraced field in an ecologically fragile area includes a hardware part, a software part, a water balance and osmosis model, a water and fertilizer optimization and adjustment model, and an industry collaboration model. The hardware part includes a terraced field intelligent irrigation equipment, soil moisture sensors, a meteorological station, a fertilizer application device, an underground water storage system, and a solar power supply. The system introduces the water balance and osmosis model to effectively manage water resources in terraced field. By integrating data from the soil sensors and meteorological station, it automatically optimizes the methods of irrigation and fertilization, preventing water waste and soil erosion. The water and fertilizer optimization and adjustment model can dynamically adjust the application of nitrogen, potassium, and phosphorus fertilizers based on real-time environmental data and crop requirements.

The invention belongs to the technical field of water-soluble composite bio-organic fertilizers, and discloses a micromolecular carbon total-nutrient composite bio-organic fertilizer for conditioning soil and a preparation method of the micromolecular carbon total-nutrient composite bio-organic fertilizer. The organic fertilizer is prepared from the following raw materials in parts by mass: 40 to 48 parts of coal, 4.3 to 7 parts of nitric acid, 8.5 to 13 parts of potassium hydroxide, 20 to 35 parts of nitrogen fertilizer, 6.5 to 10 parts of phosphate fertilizer, 0.3 to 0.6 part of dispersing agent, 1.2 to 2.0 parts of amino acid, 0.04 to 0.08 part of trace element and 0.25 to 0.55 part of fungus bag. The prepared organic water-soluble fertilizer is high in humic acid content which is more than 4 times of the existing industrial standard NY1106-2010, good in water solubility, convenient to use, capable of being applied in a flushing mode, a drip irrigation mode, a spray irrigation mode, a flying prevention mode and the like, simple in technological process, free of dust, waste water, waste gas and waste residues in the production process, environmentally friendly, efficient, easy to operate and suitable for large-scale industrial production.

This invention relates to the field of fertilizer technology, specifically to a disease-resistant microbial compound fertilizer and its preparation method. The disease-resistant microbial compound fertilizer comprises the following raw materials in parts by weight: 10-15 parts nitrogen fertilizer, 10-20 parts phosphorus fertilizer, 6-10 parts potassium fertilizer, 50-70 parts fermented organic matter, 8-12 parts fertilizer efficiency enhancer, 5-8 parts disease-resistant composition, and 6-10 parts chitosan oligosaccharide. The disease-resistant composition is a mixture of sodium selenite and potassium humate in a weight ratio of 1:1.5-2.5. The microbial compound fertilizer prepared by this invention not only achieves a combination of rapid and long-lasting effects, improving fertilizer utilization, but also increases organic matter, activates fixed phosphorus and potassium elements in the soil, and improves the micro-ecological environment. Furthermore, through the synergistic effect of Trichoderma harzianum inoculum, Paecilomyces lilacinus, the disease-resistant composition, and chitosan oligosaccharide, it exhibits good inhibitory effects on soil-borne diseases and some foliar diseases, promoting robust crop growth and improving both crop yield and quality.

The present application relates to the technical field of corn fertilization, and provides a corn whole growth period precise fertilization method, which comprises the following steps: S1, applying base fertilizer containing nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer, brown algal polysaccharide, calcium ammonium nitrate, zinc citrate, potassium humate and gamma-polyglutamic acid between corn rows when corn is sowed; S2, spraying leaf fertilizer containing potassium dihydrogen phosphate, magnesium sulfate, alginic acid and polyaspartic acid during the corn filling stage; the base fertilizer is slow-release fertilizer, and after granulation, the base fertilizer is coated with a polyester composite layer and a wax layer in sequence. According to the present application, the base fertilizer is made into double-layer coated slow-release particles and applied with sowing, the outer layer of furfural / wax forms a hydrophobic barrier to inhibit the burst release and salt damage during the seedling stage, and the inner layer of polyester composite layer forms controllable water channels and slowly hydrolyzes with time, so that nutrients are supplied in stages in a platform mode during the corn growth period, and precise fertilization and efficient utilization are achieved during the whole growth period.

The invention discloses a method for recovering vegetation of a mildly-degraded Coilin grassland. The method comprises the following steps: S1, investigating plant community characteristics and biomass; s2, determining the quality of the forage grass in the plant community; s3, collecting a soil sample; s4, reseeding: grass seeds are reseeded on the mildly degraded Coilin grassland, the reseeded grass seeds are agropyron perennioides and adsura adsura, and the mass ratio of the agropyron perennioides to the adsura adsura is (6-8): (2.5-3.5); the reseeding amount is 10-20 kg / hm < 2 >; s5, fertilization: applying a fertilizer bag, the fertilizer bag being a nitrogen-phosphorus-potassium mixed fertilizer, the nitrogen-phosphorus-potassium mixed fertilizer comprising a nitrogen fertilizer, a phosphorus fertilizer and a potash fertilizer, according to the plant community conditions of the mildly degraded Coilsein grassland, through reseeding and reasonable fertilization, the monoennial and biennial plants, perennial gramineae, hybrid grass and perennial leguminous plants of the Coilsein grassland can all grow, the yield of the Coilsein grassland is increased, and the yield of the Coilsein grassland is increased. And the pH value of the soil tends to be neutral, so that the mildly degraded Coilin grassland vegetation is recovered.

The application belongs to the field of plant genetic engineering, and particularly relates to application of a rice-derived OsDREB1 gene in enhancing the low-phosphorus stress tolerance of soybeans. A recombinant expression vector of the OsDREB1 gene is constructed, and the soybean plants are transformed by using an agrobacterium-mediated method to obtain a transgenic soybean line with stable heredity. It is found through experiments that overexpression of the OsDREB1 gene in soybeans can enhance the low-phosphorus tolerance of the soybeans, and under the same conditions, the number of pods per plant and the weight of 100 seeds of the transgenic plants are higher than those of a wild control group. The growth performance of soybeans in low-phosphorus soil is improved by using genetic engineering technology, and a new strategy for reducing the dependence of agriculture on phosphorus fertilizer is provided.

A selenium-rich Chinese wolfberry planting method comprises the steps that Chinese wolfberry tree planting pits are dug in a selected selenium sand melon planting area, and soil layers and gravel and gravel layers near the Chinese wolfberry tree planting pits are classified and collected in the length extension direction of Chinese wolfberry tree plant spacing, so that a fertilizer and soil mixing platform is formed on one side of each Chinese wolfberry tree planting pit; putting the Chinese wolfberry tree plants into the Chinese wolfberry tree planting holes, scattering a predetermined amount of decomposed organic fertilizer and phosphate fertilizer on the fertilizer-soil mixing platform, scattering the collected soil on the organic fertilizer and phosphate fertilizer on the fertilizer-soil mixing platform, and then backfilling the organic fertilizer, phosphate fertilizer and soil on the fertilizer-soil mixing platform into the Chinese wolfberry tree planting holes; root fixing water is poured into the lycium barbarum tree planting pits; the gravel and gravel layer is backfilled on the lycium barbarum tree planting holes and the fertilizer and soil mixing platform, so that lycium barbarum tree plants are stably planted, the air permeability of soil around the lycium barbarum trees is guaranteed, and the soil moisture conservation effect is improved; during field management of the Chinese wolfberry trees, organic selenium fertilizer is sprayed on leaf surfaces in the growing season of the Chinese wolfberry trees.

This invention discloses a high-calcium suspension fertilizer, its preparation method, and its application, relating to the field of fertilizer production technology. By weight, it comprises 20-30 parts of macro-element fertilizer, 10-15 parts of medium-element fertilizer, 3-5 parts of micro-element fertilizer, 0.5-3 parts of auxiliary agent, and 30-50 parts of deionized water. The macro-element fertilizer consists of 30-50 parts of nitrogen fertilizer, 20-30 parts of phosphorus fertilizer, and 15-20 parts of potassium fertilizer. The medium-element fertilizer is modified nano-calcium carbonate and magnesium carbonate. The micro-element fertilizer consists of zinc amino acid, sodium tetraborate, sodium iron ethylenediaminetetraacetate, sodium molybdenum ethylenediaminetetraacetate, manganese sulfate monohydrate, and copper sulfate pentahydrate. Through this method, nano-calcium carbonate can be rapidly absorbed by plants, enabling timely replenishment of calcium ions during plant growth and accelerating plant growth.

The present application provides a sludge treatment process for synchronously recycling carbon source and phosphorus. The sludge treatment process of the present application comprises: adding sludge to be treated into a pretreatment tank to adjust the sludge to be treated to be acidic or alkaline, pretreating the sludge to be treated to obtain pretreated sludge, and then performing anaerobic fermentation. The sludge treatment process of the present application can simultaneously realize effective recycling and utilization of the carbon source and phosphorus in the sludge. The recycled anaerobic fermentation liquid can be used for carbon source required for denitrification and phosphorus removal in a biological tank. The use of the anaerobic fermentation liquid as carbon source can obviously improve the abundance of short-cut denitrifying bacteria and denitrifying bacteria in the biological tank, obviously reduce the abundance of filamentous bacteria, and increase the types of microorganisms. The biological carbon prepared from the sludge after fermentation can be used as landscape phosphorus fertilizer, and good economic benefits can be achieved. The sludge treatment process of the present application realizes zero solid waste discharge, and completely reduces the residual sludge, thereby solving the problems of high disposal cost and insufficient disposal capacity of residual sludge in a sewage plant.

The invention relates to the technical field of soil fertility improvement, and discloses a soil fertility improvement method for supplementing cultivated land soil. The soil fertility improvement method comprises the following steps: S1, applying a base fertilizer I in a wheat season, and dressing a quick-acting inorganic nitrogen fertilizer or a phosphorus-potassium compound fertilizer; s2, after wheat is harvested, straws are separated from the field, and wheat stubbles are ploughed after a decomposition agent is added; s3, base fertilizer II is applied in the soybean season, and quick-acting inorganic nitrogen fertilizer and phosphorus-potassium compound fertilizer are topdressed; s4, after the soybeans are harvested, straw is separated from the field, and stubbles are smashed and returned to the field; wherein in the step S1, the base fertilizer I comprises decomposed manure and a potassium sulfate type compound fertilizer; in the step S2, the base fertilizer II comprises decomposed manure, a quick-acting inorganic nitrogen fertilizer, a water-soluble phosphate fertilizer and a potash fertilizer; in the base fertilizer I and the base fertilizer II, the decomposed manure is prepared by fermenting animal manure, straws, mushroom residues and a complex microbial inoculant. By means of the soil fertility improving method, the nutrient indexes such as soil organic matter and total nitrogen can be obviously improved, and the good soil fertility improving effect is achieved.

The invention provides a preparation method and application of biochar immobilized phytase, and relates to the technical field of biological enzyme immobilization. The method comprises the following steps: firing collected pineapple leaves into biochar for later use; adding pectin into water to obtain a pectin aqueous solution; adding the charcoal component into the pectin aqueous solution, uniformly mixing, filtering, washing and drying to obtain pectin charcoal; adding a cross-linking agent into the pectin biochar for cross-linking, then stirring and mixing with a phytase solution, filtering, and freeze-drying to obtain the biochar immobilized phytase. The biochar immobilized phytase can effectively overcome the defects that exogenous phytase is prone to inactivation and loss and difficult to store in soil, the phosphorus dissolving effect of phytase is enhanced, meanwhile, the biological activity and richness of indigenous phosphate-solubilizing bacteria are increased, soil phosphorus circulation is accelerated, soil phosphorus pool mineralization is promoted in the biochemical aspect, and the phosphorus-solubilizing effect is improved. The soil conditioner is a more environment-friendly and efficient soil conditioner and phosphate fertilizer synergist.

The invention discloses a nutrient signal substance synergistic controlled-release fertilizer for promoting efficient absorption of phosphorus in rice, and belongs to the technical field of controlled-release fertilizer production. Three technical breakthroughs are realized by constructing a'microbial response outer membrane layer-enzyme response inner membrane layer 'dual biological response controlled release system and cooperating with an'organelle targeted phytic acid synthesis signal guide chain substance': firstly, phosphorus release is synchronized with a rhizosphere activity period through the microbial response characteristic of an outer membrane; secondly, accurate release of signal substances in the filling stage is ensured through an inner layer film laccase triggering mechanism; 3, an endogenous signal channel of the plant is activated by utilizing an IP3 analogue precursor molecule, so that the synthesis of phytic acid is enhanced from a metabolism level, and a'grain bank strong 'is created to pull the absorption and transport of phosphorus, thereby systematically improving the utilization efficiency of the phosphate fertilizer and realizing normal form transformation from'passive supply' to'active demand '.

The application discloses a kind of full solid waste mine repair soil and preparation method thereof, it is related to soil repair technical field, the full solid waste mine repair soil includes the following weight content of raw material composition: titanium gypsum 50~80%, converter steel slag 15~30%, titanium extraction tailings 5~15%, sintering machine head ash 0~5%.In the application, titanium gypsum contains fine-grained calcium sulfate dihydrate, iron hydroxide, etc., has good water retention effect;Converter steel slag contains P, can provide phosphorus fertilizer effect for soil, and the alkalinity of steel slag can adjust the acidity and alkalinity of soil;Secondly, steel slag and titanium extraction tailings jointly act, play the role of solidifying soil and preventing water and soil loss;The pozzolanic activity of titanium extraction tailings is good, and forms strength matrix with steel slag, can solidify soil;Sintering machine head ash contains K, can provide potassium fertilizer effect for soil.

The application provides a method for preventing early decline of densely-planted double-layered circular table-shaped macadamia nut trees, and belongs to the technical field of fruit tree cultivation. The method for preventing early decline comprises hierarchical nutrient supply and flower and fruit protection management. The hierarchical nutrient supply comprises: deep application of calcium, magnesium and phosphorus fertilizer in the lower layer of more than 60cm soil, and covering with a root control device; application of organic fertilizer and biological bacteria agent in the middle layer of 30-60cm soil; and application of a large amount of elements, humic acid and seaweed extract water-soluble fertilizer in the upper layer of 0-30cm soil. The flower and fruit protection management comprises: spraying ABA and paclobutrazol during flower bud differentiation period; spraying brassinolide and fluid boron during full flowering period; spraying forchlorfenuron and amino acid during young fruit period; and spraying NAA and potassium dihydrogen phosphate during fruit swelling period. The application supplies nutrients hierarchically and protects flowers and fruits for densely-planted double-layered circular table-shaped macadamia nut trees, prevents early decline of the densely-planted macadamia nut trees, promotes the development of lower branches, balances tree vigor and maintains high yield.

The invention discloses a low-cost high-purity potassium fluoborate preparation method based on ammonium fluoride, and belongs to the field of inorganic salt chemical industry, the method comprises the following steps: S1, reacting by-product ammonium fluoride with the purity of more than or equal to 99% with boric acid to synthesize an ammonium fluoborate solution; s2, at 60-80 DEG C, precisely dropwise adding a potassium chloride solution into the ammonium fluoborate solution at a rate of 1-2 mL / (min.L), and carrying out a replacement reaction; s3, performing crystallization and purification by adopting a two-stage gradient cooling program from fast to slow; s4, a low-temperature saturated potassium fluoborate solution and absolute ethyl alcohol are sequentially adopted for targeted washing, and impurities such as chloride ions are effectively removed; and S5, performing vacuum drying to obtain a product. According to the method, the phosphorus fertilizer industrial byproduct ammonium fluoride is originally adopted to replace expensive hydrogen fluoride or fluoboric acid, so that the raw material cost is reduced by 90% or above; meanwhile, impurity inclusion is effectively inhibited, the problem of impurity introduction caused by byproduct raw materials is solved, and the electronic-grade high-purity potassium fluoborate with the main content larger than or equal to 99.95% and the chloride ion smaller than or equal to 10 ppm can be stably prepared.

The invention belongs to the technical field of soil improvement, and particularly relates to a layered granular phosphate fertilizer for acid soil as well as a preparation method and application of the layered granular phosphate fertilizer. The outer-layer fertilizer source is wrapped outside the inner-layer fertilizer particles; the inner-layer fertilizer particles are prepared from a phosphorus source, a biostimulant, nano silicon dioxide and cellulose; the mass ratio of the phosphorus source to the biostimulant to the nano silicon dioxide to the cellulose is 95: 5: (1-2): (2-4); the outer-layer fertilizer source is prepared from a soil conditioner, polylactic acid and cellulose; the mass ratio of the soil conditioner to the polylactic acid to the cellulose is 200: (1-2): (1-2). Through layered structure design and a multi-component synergistic effect, the technical bottleneck of acid soil phosphate fertilizer application is broken through from multiple dimensions of physical properties, nutrition supply, soil improvement and the like, and the acid soil phosphate fertilizer has high efficiency, environmental protection property and practicability and has remarkable popularization value.

The invention provides lodging-resistant pesticide-fertilizer granules for sugarcanes and a preparation method of the lodging-resistant pesticide-fertilizer granules, and relates to the technical field of pesticide-fertilizer granules. The medical fertilizer granule provided by the invention is prepared from the following components in percentage by weight: 0.8 to 1.2 percent of cyfluthrin-clothianidin complex, 0.4 to 0.6 percent of synergist, 1 to 3 percent of binder and the balance of slow-release chemical fertilizer, the slow-release chemical fertilizer comprises a slow-release matrix and a lodging-resistant chemical fertilizer filled in the slow-release matrix, and the lodging-resistant chemical fertilizer at least comprises a nitrogen fertilizer, a phosphorus fertilizer, a potassium fertilizer and a silicon fertilizer. According to the invention, pests in sugarcane growth can be inhibited, the absorption capability of sugarcanes on nutrients of the chemical fertilizer is improved, and the lodging resistance of sugarcanes in severe weather is effectively improved.

This invention discloses a differentiated fertilization method for Scutellaria baicalensis, belonging to the field of traditional Chinese medicinal herb cultivation technology. This method selects a corresponding ratio from multiple predetermined nitrogen, phosphorus, and potassium fertilizer application ratios based on the accumulation requirements of the target medicinal components, thereby achieving targeted regulation of different medicinal components. The multiple predetermined nitrogen-phosphorus-potassium ratios include a first ratio that increases baicalin, a second ratio that increases baicalein, a third ratio that increases wogonin, and a fourth ratio that increases wogonein. This invention allows for flexible selection of ratios according to production targets, achieving precise regulation of different medicinal components while simultaneously increasing Scutellaria baicalensis yield, and has good prospects for widespread application.

The invention discloses an application of a ZmWRKY18 gene and an encoding protein thereof in improving phosphorus absorption efficiency under corn nutrient stress. The nucleotide sequence of the ZmWRKY18 gene is as shown in SEQ ID No: 1, and the amino acid sequence of the protein of the ZmWRKY18 gene is as shown in SEQ ID No: 2. Experiments prove that the ZmWRKY18 gene plays an important regulation and control role in phosphate absorption of corn root systems under the conditions of low phosphorus and zinc deficiency, and the utilization rate of phosphate fertilizer is increased.

The invention relates to a complete-element bio-organic fertilizer suitable for loess plateau rain-fed agriculture. The complete-element bio-organic fertilizer is prepared from a composted material, a composite plant growth-promoting microbial agent, coated urea, potassium sulfate, boron and zinc trace elements, an anionic polyacrylamide-bentonite compound water-retaining agent and ammonium humate. The rotten material is prepared by fermenting and rotting sheep manure, shiitake mushroom residues, modified mushroom residue biochar and phosphate fertilizer through a composite high-temperature degradation bacterial agent; the compound plant growth promoting bacterial agent is prepared from bacillus subtilis LDB01, bacillus mucilaginosus and trichoderma harzianum Trichoderma harzianum T-22, and the compound plant growth promoting bacterial agent is prepared from bacillus subtilis LDB01, bacillus mucilaginosus and trichoderma harzianum T-22, and the compound plant growth promoting bacterial agent is prepared from bacillus subtilis LDB01, bacillus mucilaginosus and trichoderma harzianum T-22. Meanwhile, the invention further discloses a preparation method of the complete-element bio-organic fertilizer. The complete-element bio-organic fertilizer is comprehensive and balanced in nutrition, the survival rate of bacteria can be increased, and drought stress is avoided.

The application discloses an application of Medicago sativa MsCEPR1 gene and protein coded by the same in low-phosphorus stress tolerance of Medicago sativa and belongs to the technical field of plant genetic engineering. The Medicago sativa MsCEPR1 gene provided in the application has a nucleotide sequence as shown in SEQ ID NO. 1. The application preliminarily reveals the regulation of CEPR1 on phosphorus absorption related genes by exploring the function of CEPR1 in phosphorus absorption, provides a basis for constructing a molecular network of CEPR1 regulating phosphorus absorption, and provides a direction for cultivating Medicago sativa varieties with high phosphorus absorption, which has important significance for reducing the dependence on phosphorus fertilizer and sustainable agricultural development.

The present application comprises a plurality of microbial treatment tanks as a primary treatment aquaculture tail water stage, and a water plant purification tank as a secondary treatment aquaculture tail water stage; wherein the aquaculture tail water enters different microbial treatment tanks through a plurality of tail water inlet pipes; an active sludge and an aeration mechanism for water purification are arranged at the bottom of the microbial treatment tank; and the water plant purification tank is connected with the plurality of microbial treatment tanks through a pipeline. In the present application, the microbial treatment tank is provided with a cover of a sandwich cover on the active sludge layer, and the aeration efficiency is improved by narrowing the space, so as to improve the water purification efficiency of the microorganisms. Meanwhile, the nitrogen and phosphorus substances after the microbial treatment can be recycled through the adsorbent filled in the sandwich cover, and used as resourceful nitrogen and phosphorus fertilizers. In addition, the water quality after the primary treatment aquaculture tail water stage is further adsorbed and treated by the water plants, so that the recycling and reuse after the water quality purification can be met.

The invention discloses a fertilizer composition and a use method thereof, and belongs to the technical field of rape planting. The fertilizer composition comprises a nitrogen fertilizer, a phosphate fertilizer and a potash fertilizer which are respectively applied to a shallow soil area and a deep soil area. The invention further provides an application method of the fertilizer composition. The application method comprises the following steps: applying the nitrogen fertilizer, the phosphate fertilizer and the potash fertilizer to a shallow area and a deep area of soil after direct seeding of rape seeds in a layered manner. The fertilizer composition provided by the invention can significantly improve the lodging resistance of rape.

The application discloses application of an ERF15 gene in regulating and controlling plant arbuscular mycorrhizal symbiosis and belongs to the technical field of biotechnology.The nucleotide sequence of the protein coding region of the ERF15 gene is shown in SEQ ID NO.1 or has at least 70% homology with the sequence shown in SEQ ID NO.1 and the coded protein is functionally equivalent.The application discloses, for the first time, positive regulation of the ERF15 gene in the plant arbuscular mycorrhizal symbiosis, analyzes the molecular mechanism of the ERF15 in promoting phosphorus absorption through the mycorrhizal symbiosis pathway, and provides gene resources and a new idea for plant molecular stress resistance genetic breeding and the like.The application has very important practical value for improving the absorption and utilization efficiency of plants on phosphorus elements, reducing the use of phosphorus fertilizer, and improving the utilization of infertile soil and the like.

The invention discloses an animal polypeptide-containing organic phosphate fertilizer and a preparation method thereof, and belongs to the technical field of organic fertilizers. The method takes animal tissue waste as a raw material, and sequentially comprises the following steps: destroying a structure through alkali pretreatment, carrying out mild acidolysis with phosphoric acid, carrying out precise enzymolysis with compound enzyme, mixing and coating a modified montmorillonite / sodium carboxymethyl cellulose gel compound with concentrated enzymatic hydrolysate and citric acid, and finally, carrying out spray drying to obtain the product. According to the method, macromolecular proteins in the wastes are efficiently converted into micromolecular active polypeptides through a multi-stage synergistic process, and coating and slow release of nutrients are realized by utilizing a functional gel carrier. The polypeptide content of the prepared organic phosphate fertilizer is greater than or equal to 2%, the nutrient release period is as long as 60 days or more, the stability is good, caking and mildewing are not easily caused, resource utilization of wastes can be realized, comprehensive and continuous nutrients can be provided for crops, and the requirements of modern agriculture on efficient and environment-friendly fertilizers are met.